Durania virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Durania phlebovirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F4ZCL0|F4ZCL0_9VIRU Nucleoprotein OS=Durania virus OX=1006585 GN=NP PE=3 SV=1
MM1 pKa = 7.42TSYY4 pKa = 11.29LFDD7 pKa = 3.42TPVIVRR13 pKa = 11.84PNGYY17 pKa = 9.24FDD19 pKa = 4.06RR20 pKa = 11.84EE21 pKa = 4.37VYY23 pKa = 7.9VTYY26 pKa = 9.66MAHH29 pKa = 6.72NKK31 pKa = 9.15YY32 pKa = 10.21CEE34 pKa = 4.27HH35 pKa = 6.27TVSVHH40 pKa = 5.22NCMEE44 pKa = 3.96IPVRR48 pKa = 11.84NFGVSMLHH56 pKa = 6.51KK57 pKa = 10.34EE58 pKa = 4.3SFSDD62 pKa = 4.96FYY64 pKa = 11.18ILGLFPFRR72 pKa = 11.84WGDD75 pKa = 3.05KK76 pKa = 9.13MGNSRR81 pKa = 11.84VTRR84 pKa = 11.84EE85 pKa = 3.97SFPFMKK91 pKa = 10.31EE92 pKa = 3.61LLADD96 pKa = 3.34ICKK99 pKa = 8.72MHH101 pKa = 7.25DD102 pKa = 3.37SEE104 pKa = 5.08FFRR107 pKa = 11.84SFLPNIKK114 pKa = 9.94KK115 pKa = 10.41ALSWPLGYY123 pKa = 9.73PSLDD127 pKa = 4.15FIRR130 pKa = 11.84MTDD133 pKa = 3.65PDD135 pKa = 3.22NSMPHH140 pKa = 5.74FRR142 pKa = 11.84EE143 pKa = 3.55ISNNATLIMQMGQPAIDD160 pKa = 4.12LDD162 pKa = 3.78QGFVNAHH169 pKa = 5.65KK170 pKa = 10.11RR171 pKa = 11.84IVMEE175 pKa = 4.48SITRR179 pKa = 11.84GFGHH183 pKa = 7.36KK184 pKa = 10.29DD185 pKa = 3.34FPGKK189 pKa = 10.25NLIFEE194 pKa = 4.48VASLQCVRR202 pKa = 11.84MLNAVPADD210 pKa = 3.79LLYY213 pKa = 11.03CQITNSLTSTLMKK226 pKa = 10.25HH227 pKa = 4.81RR228 pKa = 11.84AEE230 pKa = 4.54MEE232 pKa = 4.14SHH234 pKa = 6.04PQGPLGNQKK243 pKa = 9.93WIPDD247 pKa = 3.95PDD249 pKa = 3.33SWLLDD254 pKa = 3.87DD255 pKa = 5.16QSSTLHH261 pKa = 6.5EE262 pKa = 4.5EE263 pKa = 3.93FNLIISDD270 pKa = 4.83SEE272 pKa = 4.73DD273 pKa = 3.27EE274 pKa = 4.93DD275 pKa = 3.93EE276 pKa = 4.77

Molecular weight:
31.89 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F4ZCL1|F4ZCL1_9VIRU Glycoprotein OS=Durania virus OX=1006585 GN=GP PE=4 SV=1
MM1 pKa = 7.94ADD3 pKa = 3.97FEE5 pKa = 4.33RR6 pKa = 11.84LAIEE10 pKa = 4.29FSEE13 pKa = 4.5AGVNIADD20 pKa = 3.43VVAWVNEE27 pKa = 3.79FAYY30 pKa = 10.49QGFDD34 pKa = 2.91AKK36 pKa = 10.46RR37 pKa = 11.84VIEE40 pKa = 4.12LLQQRR45 pKa = 11.84GGTSWQEE52 pKa = 4.17DD53 pKa = 3.64ARR55 pKa = 11.84KK56 pKa = 9.14MIVLALTRR64 pKa = 11.84GNKK67 pKa = 7.82IEE69 pKa = 4.12KK70 pKa = 9.33MIMKK74 pKa = 9.73MSEE77 pKa = 3.91EE78 pKa = 4.12GKK80 pKa = 8.59KK81 pKa = 8.63TVLSLKK87 pKa = 10.13KK88 pKa = 10.14RR89 pKa = 11.84YY90 pKa = 7.5QLKK93 pKa = 10.3SGNPGRR99 pKa = 11.84DD100 pKa = 3.45DD101 pKa = 3.54LTLSRR106 pKa = 11.84VAAALAGWTCQALPHH121 pKa = 6.37VEE123 pKa = 3.94NFLPVTGEE131 pKa = 3.97AMDD134 pKa = 4.46ALSPSFPRR142 pKa = 11.84CMMHH146 pKa = 6.97PSFAGLVDD154 pKa = 3.48TTLPPEE160 pKa = 4.23TQDD163 pKa = 4.51AILAAHH169 pKa = 6.45SLFLVQFSKK178 pKa = 11.16VINPGLRR185 pKa = 11.84GKK187 pKa = 10.37AKK189 pKa = 10.37SEE191 pKa = 3.98VVASFKK197 pKa = 10.94QPMLAAVNGGFIGPEE212 pKa = 3.92KK213 pKa = 9.54RR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 8.28MLQSLGVVDD225 pKa = 4.19VNGIPSEE232 pKa = 4.11AVKK235 pKa = 10.75VAAQKK240 pKa = 10.28FRR242 pKa = 11.84ASQQ245 pKa = 2.95

Molecular weight:
26.78 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3926

245

2085

981.5

110.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.323 ± 1.088

2.878 ± 0.852

5.96 ± 0.487

6.623 ± 0.378

4.712 ± 0.366

5.935 ± 0.276

2.598 ± 0.291

6.47 ± 0.528

6.393 ± 0.346

8.839 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.184 ± 0.64

3.49 ± 0.341

3.948 ± 0.424

3.082 ± 0.32

5.527 ± 0.214

9.883 ± 0.726

4.992 ± 0.275

6.342 ± 0.411

1.248 ± 0.124

2.573 ± 0.343

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski