Sphingorhabdus sp. GY_G

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingorhabdus; unclassified Sphingorhabdus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371BFI1|A0A371BFI1_9SPHN 6-phosphogluconolactonase OS=Sphingorhabdus sp. GY_G OX=2292257 GN=pgl PE=3 SV=1
MM1 pKa = 7.79AEE3 pKa = 3.63PFKK6 pKa = 10.61RR7 pKa = 11.84YY8 pKa = 9.42RR9 pKa = 11.84CTYY12 pKa = 9.79CGQEE16 pKa = 3.83YY17 pKa = 10.81DD18 pKa = 4.32EE19 pKa = 5.65AEE21 pKa = 4.09GWPDD25 pKa = 4.01DD26 pKa = 4.65GIAPGTRR33 pKa = 11.84WEE35 pKa = 4.66DD36 pKa = 3.87VPDD39 pKa = 3.5DD40 pKa = 4.76WYY42 pKa = 11.48CPTCGAEE49 pKa = 4.01KK50 pKa = 10.67ADD52 pKa = 3.75FEE54 pKa = 4.44MVEE57 pKa = 4.12IGG59 pKa = 4.15

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371BHX6|A0A371BHX6_9SPHN Delta-aminolevulinic acid dehydratase OS=Sphingorhabdus sp. GY_G OX=2292257 GN=DXH95_07245 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTAAGRR28 pKa = 11.84AILNGRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.04KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3125

0

3125

992764

40

4340

317.7

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.303 ± 0.057

0.843 ± 0.017

5.874 ± 0.039

5.755 ± 0.041

3.788 ± 0.03

8.646 ± 0.042

1.936 ± 0.023

5.483 ± 0.029

3.934 ± 0.039

9.66 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.025

3.019 ± 0.032

4.989 ± 0.027

3.222 ± 0.022

6.404 ± 0.044

5.684 ± 0.033

5.167 ± 0.054

6.918 ± 0.031

1.45 ± 0.02

2.343 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski