California sea lion adenovirus 1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Mastadenovirus; Sea lion mastadenovirus A

Average proteome isoelectric point is 4.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5NTE2|A0A7D5NTE2_9ADEN IX OS=California sea lion adenovirus 1 OX=943083 PE=3 SV=1
MM1 pKa = 7.67PLITLHH7 pKa = 6.5FSKK10 pKa = 10.74EE11 pKa = 3.95LLEE14 pKa = 4.19MVANCLPEE22 pKa = 4.56DD23 pKa = 3.85ARR25 pKa = 11.84PPHH28 pKa = 7.01PYY30 pKa = 10.61DD31 pKa = 4.72LLDD34 pKa = 4.86LEE36 pKa = 5.1HH37 pKa = 6.6QPSLHH42 pKa = 7.02DD43 pKa = 3.57MFDD46 pKa = 3.12IVLDD50 pKa = 3.76SDD52 pKa = 4.13VGGEE56 pKa = 4.14TPEE59 pKa = 4.62IEE61 pKa = 4.57LDD63 pKa = 3.8FPEE66 pKa = 4.47TLVNDD71 pKa = 3.92ALDD74 pKa = 3.96YY75 pKa = 10.17FASSPISIPSDD86 pKa = 3.5SSPVSPDD93 pKa = 3.52LCCHH97 pKa = 6.61EE98 pKa = 5.09SPLSSVSEE106 pKa = 4.29AEE108 pKa = 4.23NEE110 pKa = 4.03DD111 pKa = 3.59TSVEE115 pKa = 4.66KK116 pKa = 10.81EE117 pKa = 4.04NQDD120 pKa = 4.2FKK122 pKa = 11.37LDD124 pKa = 3.75CPDD127 pKa = 3.58YY128 pKa = 11.06PGVNCTSCDD137 pKa = 3.43YY138 pKa = 10.81HH139 pKa = 7.89RR140 pKa = 11.84QANPEE145 pKa = 4.17AVCSLCYY152 pKa = 9.87MRR154 pKa = 11.84ATSHH158 pKa = 7.22LIFEE162 pKa = 4.88PVSPPPVSEE171 pKa = 4.68DD172 pKa = 3.35VFDD175 pKa = 5.22FDD177 pKa = 5.14VDD179 pKa = 4.0TVLDD183 pKa = 4.01DD184 pKa = 6.22LIAEE188 pKa = 4.61KK189 pKa = 11.01DD190 pKa = 3.4PFLSFEE196 pKa = 4.38EE197 pKa = 4.16VDD199 pKa = 5.46ASLQCLANGANSLFTLDD216 pKa = 3.89SAPASTLKK224 pKa = 10.52RR225 pKa = 11.84KK226 pKa = 9.79HH227 pKa = 6.98DD228 pKa = 5.03DD229 pKa = 3.79DD230 pKa = 5.56DD231 pKa = 3.95QLEE234 pKa = 4.36PLDD237 pKa = 4.43LSLPKK242 pKa = 10.23KK243 pKa = 10.53SCC245 pKa = 3.34

Molecular weight: 27.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059XN88|A0A059XN88_9ADEN E1A OS=California sea lion adenovirus 1 OX=943083 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 4.25QLKK5 pKa = 10.59DD6 pKa = 3.34YY7 pKa = 11.33SFFKK11 pKa = 10.69EE12 pKa = 4.46VVEE15 pKa = 4.23HH16 pKa = 6.98ASLRR20 pKa = 11.84TGNLYY25 pKa = 10.61RR26 pKa = 11.84FFFSSSLAKK35 pKa = 10.56VVFQVYY41 pKa = 9.71LEE43 pKa = 4.22KK44 pKa = 11.04VGEE47 pKa = 3.95FDD49 pKa = 5.36RR50 pKa = 11.84LDD52 pKa = 3.63STASALLALSQGNNFLFEE70 pKa = 4.14KK71 pKa = 10.66LIIPEE76 pKa = 4.13LPFYY80 pKa = 11.23SFGHH84 pKa = 6.11ILASLAFCSFCVNKK98 pKa = 10.38ADD100 pKa = 3.76ISATVSSGFILDD112 pKa = 4.17AVCRR116 pKa = 11.84PAWQKK121 pKa = 11.29FMMSHH126 pKa = 6.5NMMEE130 pKa = 4.25NQTGSSEE137 pKa = 4.14FSLEE141 pKa = 4.29EE142 pKa = 4.11ALTDD146 pKa = 3.58NN147 pKa = 4.96

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

966

147

418

241.5

25.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.797 ± 0.684

3.623 ± 0.951

7.557 ± 1.938

7.35 ± 0.389

4.969 ± 1.269

3.727 ± 0.701

2.484 ± 0.261

4.348 ± 0.803

4.037 ± 0.726

9.006 ± 1.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.359

4.762 ± 0.629

5.383 ± 1.433

3.106 ± 1.368

2.692 ± 0.407

9.317 ± 1.003

4.141 ± 0.241

6.936 ± 0.703

0.518 ± 0.163

2.484 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski