Bacillus phage Juan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Northropvirinae; Claudivirus; Bacillus virus Juan

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223LKJ7|A0A223LKJ7_9CAUD Uncharacterized protein OS=Bacillus phage Juan OX=2023949 PE=4 SV=1
MM1 pKa = 7.74ALQTITLDD9 pKa = 3.59FTGKK13 pKa = 9.92IAGSTTQNPNIAYY26 pKa = 9.95FNDD29 pKa = 3.43VEE31 pKa = 4.58FTQLQYY37 pKa = 11.44DD38 pKa = 3.72QIFGSEE44 pKa = 4.13LRR46 pKa = 11.84VATTDD51 pKa = 3.23VLKK54 pKa = 11.09FEE56 pKa = 4.87FPVPTEE62 pKa = 3.8AQINLQSVGIYY73 pKa = 9.97YY74 pKa = 10.4KK75 pKa = 10.76SKK77 pKa = 10.67LDD79 pKa = 3.44SGEE82 pKa = 4.08NEE84 pKa = 4.25LRR86 pKa = 11.84SSDD89 pKa = 3.28KK90 pKa = 11.04GVYY93 pKa = 10.19NDD95 pKa = 4.21DD96 pKa = 3.34TTTYY100 pKa = 9.55KK101 pKa = 10.57QKK103 pKa = 10.78LLEE106 pKa = 4.48IYY108 pKa = 10.65GIDD111 pKa = 4.54IQDD114 pKa = 3.25QTIKK118 pKa = 10.89FNITTTGDD126 pKa = 3.36YY127 pKa = 10.86GVYY130 pKa = 9.45EE131 pKa = 4.48VKK133 pKa = 10.76CDD135 pKa = 3.52VTYY138 pKa = 11.34DD139 pKa = 3.67DD140 pKa = 5.31AGLNIYY146 pKa = 9.46GGQYY150 pKa = 9.94QYY152 pKa = 11.49SDD154 pKa = 3.1NGGVVVTVPIQNKK167 pKa = 9.11KK168 pKa = 10.52LEE170 pKa = 4.68EE171 pKa = 3.95ITTEE175 pKa = 4.22LEE177 pKa = 3.94GRR179 pKa = 11.84PTNSVTNVTFAQVGTEE195 pKa = 3.92LVPP198 pKa = 4.06

Molecular weight: 22.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223LJC9|A0A223LJC9_9CAUD Upper collar connector OS=Bacillus phage Juan OX=2023949 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84RR3 pKa = 11.84NRR5 pKa = 11.84RR6 pKa = 11.84PNKK9 pKa = 9.72NKK11 pKa = 10.22CNNLMSLTNKK21 pKa = 9.3EE22 pKa = 3.94CFYY25 pKa = 11.84YY26 pKa = 10.41MLTHH30 pKa = 6.33HH31 pKa = 6.94MKK33 pKa = 10.73RR34 pKa = 11.84NEE36 pKa = 3.78NKK38 pKa = 9.98INGLIKK44 pKa = 10.47DD45 pKa = 3.75NKK47 pKa = 8.54EE48 pKa = 4.14SKK50 pKa = 10.43RR51 pKa = 11.84LMGILEE57 pKa = 4.39KK58 pKa = 10.88EE59 pKa = 4.49LFSKK63 pKa = 10.91

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

6900

46

726

202.9

23.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.42 ± 0.434

1.145 ± 0.194

6.551 ± 0.309

8.145 ± 0.719

4.551 ± 0.388

6.58 ± 0.555

1.536 ± 0.165

6.797 ± 0.373

9.478 ± 0.611

6.783 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.333 ± 0.297

7.116 ± 0.448

2.435 ± 0.263

3.333 ± 0.232

4.043 ± 0.337

5.246 ± 0.266

6.348 ± 0.564

6.232 ± 0.406

1.188 ± 0.159

4.739 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski