Streptococcus satellite phage Javan54

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUU5|A0A4D5ZUU5_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan54 OX=2558733 GN=JavanS54_0005 PE=3 SV=1
MM1 pKa = 7.78ADD3 pKa = 3.36KK4 pKa = 11.02NFNLAEE10 pKa = 3.96ARR12 pKa = 11.84AEE14 pKa = 4.43NFGKK18 pKa = 9.7WLDD21 pKa = 3.76EE22 pKa = 4.59AYY24 pKa = 9.05EE25 pKa = 4.18TMLDD29 pKa = 4.38FSLEE33 pKa = 4.24DD34 pKa = 4.04KK35 pKa = 10.56FDD37 pKa = 4.04CYY39 pKa = 11.02SIEE42 pKa = 4.16EE43 pKa = 4.09QNQLDD48 pKa = 3.89RR49 pKa = 11.84VLEE52 pKa = 4.21TLCDD56 pKa = 4.5FSDD59 pKa = 3.36MWEE62 pKa = 3.95KK63 pKa = 10.94GQIIVSSKK71 pKa = 8.93EE72 pKa = 3.94RR73 pKa = 11.84EE74 pKa = 3.95VTEE77 pKa = 4.09

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZV16|A0A4D5ZV16_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan54 OX=2558733 GN=JavanS54_0021 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84NAKK5 pKa = 7.83EE6 pKa = 3.85LRR8 pKa = 11.84EE9 pKa = 4.66GISMLFFVLIVADD22 pKa = 4.12GAGKK26 pKa = 10.43SILILARR33 pKa = 11.84GMLAVSAKK41 pKa = 10.31RR42 pKa = 11.84YY43 pKa = 8.54INKK46 pKa = 9.4HH47 pKa = 4.28

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

4249

41

498

146.5

17.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.554 ± 0.336

0.871 ± 0.203

5.531 ± 0.342

7.625 ± 0.584

4.636 ± 0.321

4.848 ± 0.42

1.718 ± 0.234

7.296 ± 0.509

9.414 ± 0.554

9.932 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.224

5.625 ± 0.499

3.036 ± 0.497

3.836 ± 0.292

5.06 ± 0.501

5.743 ± 0.564

5.978 ± 0.373

4.731 ± 0.338

1.224 ± 0.171

4.895 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski