Amino acid dipepetide frequency for Mycobacterium phage Lorde

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.174AlaAla: 14.174 ± 1.53
1.029AlaCys: 1.029 ± 0.293
6.287AlaAsp: 6.287 ± 0.632
7.658AlaGlu: 7.658 ± 0.755
2.629AlaPhe: 2.629 ± 0.381
9.373AlaGly: 9.373 ± 1.219
2.515AlaHis: 2.515 ± 0.427
3.944AlaIle: 3.944 ± 0.497
3.886AlaLys: 3.886 ± 0.515
7.944AlaLeu: 7.944 ± 0.756
2.629AlaMet: 2.629 ± 0.401
3.429AlaAsn: 3.429 ± 0.452
5.144AlaPro: 5.144 ± 0.639
3.429AlaGln: 3.429 ± 0.493
7.258AlaArg: 7.258 ± 0.627
5.487AlaSer: 5.487 ± 0.691
6.915AlaThr: 6.915 ± 0.697
6.801AlaVal: 6.801 ± 0.769
2.629AlaTrp: 2.629 ± 0.372
2.286AlaTyr: 2.286 ± 0.309
0.0AlaXaa: 0.0 ± 0.0
Cys
1.086CysAla: 1.086 ± 0.31
0.114CysCys: 0.114 ± 0.08
1.143CysAsp: 1.143 ± 0.332
0.514CysGlu: 0.514 ± 0.186
0.286CysPhe: 0.286 ± 0.153
1.315CysGly: 1.315 ± 0.315
0.286CysHis: 0.286 ± 0.127
0.171CysIle: 0.171 ± 0.094
0.514CysLys: 0.514 ± 0.193
0.686CysLeu: 0.686 ± 0.23
0.114CysMet: 0.114 ± 0.083
0.4CysAsn: 0.4 ± 0.146
1.257CysPro: 1.257 ± 0.278
0.457CysGln: 0.457 ± 0.19
0.972CysArg: 0.972 ± 0.269
0.972CysSer: 0.972 ± 0.305
0.857CysThr: 0.857 ± 0.262
0.686CysVal: 0.686 ± 0.186
0.229CysTrp: 0.229 ± 0.11
0.229CysTyr: 0.229 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
6.973AspAla: 6.973 ± 0.641
0.914AspCys: 0.914 ± 0.219
4.858AspAsp: 4.858 ± 0.481
3.143AspGlu: 3.143 ± 0.399
1.372AspPhe: 1.372 ± 0.256
6.287AspGly: 6.287 ± 0.512
1.315AspHis: 1.315 ± 0.251
2.629AspIle: 2.629 ± 0.47
1.715AspLys: 1.715 ± 0.367
5.487AspLeu: 5.487 ± 0.601
1.257AspMet: 1.257 ± 0.271
2.0AspAsn: 2.0 ± 0.388
5.715AspPro: 5.715 ± 0.594
2.229AspGln: 2.229 ± 0.376
5.601AspArg: 5.601 ± 0.757
3.201AspSer: 3.201 ± 0.485
3.829AspThr: 3.829 ± 0.393
4.229AspVal: 4.229 ± 0.579
1.772AspTrp: 1.772 ± 0.268
1.829AspTyr: 1.829 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
6.058GluAla: 6.058 ± 0.698
1.029GluCys: 1.029 ± 0.29
3.258GluAsp: 3.258 ± 0.416
3.143GluGlu: 3.143 ± 0.435
2.286GluPhe: 2.286 ± 0.358
3.315GluGly: 3.315 ± 0.417
1.543GluHis: 1.543 ± 0.424
2.743GluIle: 2.743 ± 0.385
2.115GluLys: 2.115 ± 0.354
5.258GluLeu: 5.258 ± 0.697
1.429GluMet: 1.429 ± 0.287
2.057GluAsn: 2.057 ± 0.309
2.572GluPro: 2.572 ± 0.423
3.258GluGln: 3.258 ± 0.44
4.629GluArg: 4.629 ± 0.48
3.486GluSer: 3.486 ± 0.398
3.944GluThr: 3.944 ± 0.601
3.772GluVal: 3.772 ± 0.531
1.143GluTrp: 1.143 ± 0.221
1.372GluTyr: 1.372 ± 0.265
0.0GluXaa: 0.0 ± 0.0
Phe
2.972PheAla: 2.972 ± 0.399
0.4PheCys: 0.4 ± 0.14
2.572PheAsp: 2.572 ± 0.358
1.543PheGlu: 1.543 ± 0.334
0.914PhePhe: 0.914 ± 0.289
3.486PheGly: 3.486 ± 0.58
0.4PheHis: 0.4 ± 0.143
1.257PheIle: 1.257 ± 0.312
1.2PheLys: 1.2 ± 0.277
1.943PheLeu: 1.943 ± 0.332
0.857PheMet: 0.857 ± 0.242
1.257PheAsn: 1.257 ± 0.323
1.657PhePro: 1.657 ± 0.341
0.857PheGln: 0.857 ± 0.308
1.715PheArg: 1.715 ± 0.279
1.943PheSer: 1.943 ± 0.292
2.286PheThr: 2.286 ± 0.338
2.057PheVal: 2.057 ± 0.298
0.629PheTrp: 0.629 ± 0.187
0.8PheTyr: 0.8 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
9.202GlyAla: 9.202 ± 1.122
1.143GlyCys: 1.143 ± 0.322
6.344GlyAsp: 6.344 ± 0.576
3.601GlyGlu: 3.601 ± 0.48
2.915GlyPhe: 2.915 ± 0.456
10.059GlyGly: 10.059 ± 2.115
2.115GlyHis: 2.115 ± 0.323
4.229GlyIle: 4.229 ± 0.634
2.686GlyLys: 2.686 ± 0.38
5.83GlyLeu: 5.83 ± 0.576
2.343GlyMet: 2.343 ± 0.45
3.086GlyAsn: 3.086 ± 0.361
4.058GlyPro: 4.058 ± 0.485
1.657GlyGln: 1.657 ± 0.439
4.687GlyArg: 4.687 ± 0.639
5.315GlySer: 5.315 ± 0.829
7.087GlyThr: 7.087 ± 0.745
6.458GlyVal: 6.458 ± 0.605
2.4GlyTrp: 2.4 ± 0.314
2.057GlyTyr: 2.057 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
2.172HisAla: 2.172 ± 0.352
0.514HisCys: 0.514 ± 0.208
1.486HisAsp: 1.486 ± 0.301
1.257HisGlu: 1.257 ± 0.271
0.4HisPhe: 0.4 ± 0.121
2.0HisGly: 2.0 ± 0.301
1.029HisHis: 1.029 ± 0.279
1.372HisIle: 1.372 ± 0.329
0.743HisLys: 0.743 ± 0.229
1.829HisLeu: 1.829 ± 0.289
0.514HisMet: 0.514 ± 0.153
0.572HisAsn: 0.572 ± 0.16
1.486HisPro: 1.486 ± 0.255
0.629HisGln: 0.629 ± 0.167
1.657HisArg: 1.657 ± 0.375
0.972HisSer: 0.972 ± 0.227
1.429HisThr: 1.429 ± 0.363
1.2HisVal: 1.2 ± 0.328
0.4HisTrp: 0.4 ± 0.132
0.972HisTyr: 0.972 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
5.087IleAla: 5.087 ± 0.566
0.629IleCys: 0.629 ± 0.24
3.486IleAsp: 3.486 ± 0.389
3.258IleGlu: 3.258 ± 0.377
1.2IlePhe: 1.2 ± 0.303
3.715IleGly: 3.715 ± 0.464
0.972IleHis: 0.972 ± 0.234
1.543IleIle: 1.543 ± 0.316
1.257IleLys: 1.257 ± 0.303
2.172IleLeu: 2.172 ± 0.419
0.457IleMet: 0.457 ± 0.163
2.0IleAsn: 2.0 ± 0.284
2.8IlePro: 2.8 ± 0.367
1.886IleGln: 1.886 ± 0.301
2.572IleArg: 2.572 ± 0.372
2.229IleSer: 2.229 ± 0.372
3.086IleThr: 3.086 ± 0.43
3.029IleVal: 3.029 ± 0.428
0.914IleTrp: 0.914 ± 0.2
1.029IleTyr: 1.029 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
4.115LysAla: 4.115 ± 0.498
0.343LysCys: 0.343 ± 0.152
1.886LysAsp: 1.886 ± 0.27
1.543LysGlu: 1.543 ± 0.268
1.029LysPhe: 1.029 ± 0.193
3.086LysGly: 3.086 ± 0.371
1.257LysHis: 1.257 ± 0.267
1.029LysIle: 1.029 ± 0.269
1.429LysLys: 1.429 ± 0.393
3.029LysLeu: 3.029 ± 0.508
0.857LysMet: 0.857 ± 0.213
0.914LysAsn: 0.914 ± 0.237
2.743LysPro: 2.743 ± 0.43
1.543LysGln: 1.543 ± 0.28
2.115LysArg: 2.115 ± 0.383
1.543LysSer: 1.543 ± 0.293
1.886LysThr: 1.886 ± 0.331
2.343LysVal: 2.343 ± 0.407
0.686LysTrp: 0.686 ± 0.217
1.029LysTyr: 1.029 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
7.258LeuAla: 7.258 ± 0.658
0.743LeuCys: 0.743 ± 0.209
5.144LeuAsp: 5.144 ± 0.619
3.886LeuGlu: 3.886 ± 0.478
2.343LeuPhe: 2.343 ± 0.333
5.83LeuGly: 5.83 ± 0.649
1.086LeuHis: 1.086 ± 0.309
3.143LeuIle: 3.143 ± 0.424
2.572LeuLys: 2.572 ± 0.393
4.515LeuLeu: 4.515 ± 0.523
1.6LeuMet: 1.6 ± 0.329
2.515LeuAsn: 2.515 ± 0.442
4.572LeuPro: 4.572 ± 0.586
3.029LeuGln: 3.029 ± 0.426
5.43LeuArg: 5.43 ± 0.683
5.372LeuSer: 5.372 ± 0.523
5.772LeuThr: 5.772 ± 0.531
5.43LeuVal: 5.43 ± 0.592
1.086LeuTrp: 1.086 ± 0.268
1.886LeuTyr: 1.886 ± 0.374
0.0LeuXaa: 0.0 ± 0.0
Met
1.943MetAla: 1.943 ± 0.336
0.057MetCys: 0.057 ± 0.05
1.315MetAsp: 1.315 ± 0.235
0.857MetGlu: 0.857 ± 0.202
0.857MetPhe: 0.857 ± 0.254
1.886MetGly: 1.886 ± 0.345
0.171MetHis: 0.171 ± 0.105
1.2MetIle: 1.2 ± 0.315
0.8MetLys: 0.8 ± 0.251
1.486MetLeu: 1.486 ± 0.196
0.572MetMet: 0.572 ± 0.256
1.086MetAsn: 1.086 ± 0.245
1.257MetPro: 1.257 ± 0.263
0.114MetGln: 0.114 ± 0.074
1.486MetArg: 1.486 ± 0.257
3.201MetSer: 3.201 ± 0.48
2.229MetThr: 2.229 ± 0.349
1.315MetVal: 1.315 ± 0.284
0.457MetTrp: 0.457 ± 0.163
0.343MetTyr: 0.343 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.372AsnAla: 3.372 ± 0.39
0.286AsnCys: 0.286 ± 0.146
1.829AsnAsp: 1.829 ± 0.29
1.943AsnGlu: 1.943 ± 0.337
0.857AsnPhe: 0.857 ± 0.27
3.543AsnGly: 3.543 ± 0.58
0.972AsnHis: 0.972 ± 0.251
1.486AsnIle: 1.486 ± 0.479
0.972AsnLys: 0.972 ± 0.277
2.458AsnLeu: 2.458 ± 0.382
0.8AsnMet: 0.8 ± 0.162
1.657AsnAsn: 1.657 ± 0.363
3.429AsnPro: 3.429 ± 0.395
1.143AsnGln: 1.143 ± 0.355
2.115AsnArg: 2.115 ± 0.415
1.715AsnSer: 1.715 ± 0.343
2.229AsnThr: 2.229 ± 0.307
1.6AsnVal: 1.6 ± 0.279
0.857AsnTrp: 0.857 ± 0.176
0.857AsnTyr: 0.857 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
6.344ProAla: 6.344 ± 0.523
0.686ProCys: 0.686 ± 0.225
4.458ProAsp: 4.458 ± 0.568
4.401ProGlu: 4.401 ± 0.487
2.172ProPhe: 2.172 ± 0.41
6.458ProGly: 6.458 ± 0.646
1.486ProHis: 1.486 ± 0.326
2.057ProIle: 2.057 ± 0.329
2.286ProLys: 2.286 ± 0.477
3.886ProLeu: 3.886 ± 0.544
1.315ProMet: 1.315 ± 0.332
2.515ProAsn: 2.515 ± 0.337
4.344ProPro: 4.344 ± 0.712
2.515ProGln: 2.515 ± 0.415
2.972ProArg: 2.972 ± 0.497
2.858ProSer: 2.858 ± 0.511
3.029ProThr: 3.029 ± 0.476
4.801ProVal: 4.801 ± 0.567
1.2ProTrp: 1.2 ± 0.301
1.715ProTyr: 1.715 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
4.744GlnAla: 4.744 ± 0.511
0.343GlnCys: 0.343 ± 0.188
1.543GlnAsp: 1.543 ± 0.289
1.886GlnGlu: 1.886 ± 0.352
1.086GlnPhe: 1.086 ± 0.253
2.057GlnGly: 2.057 ± 0.385
0.8GlnHis: 0.8 ± 0.213
1.715GlnIle: 1.715 ± 0.344
1.543GlnLys: 1.543 ± 0.218
3.372GlnLeu: 3.372 ± 0.42
0.514GlnMet: 0.514 ± 0.164
0.686GlnAsn: 0.686 ± 0.196
2.0GlnPro: 2.0 ± 0.454
1.943GlnGln: 1.943 ± 0.491
2.572GlnArg: 2.572 ± 0.383
2.572GlnSer: 2.572 ± 0.376
1.543GlnThr: 1.543 ± 0.33
2.286GlnVal: 2.286 ± 0.392
0.686GlnTrp: 0.686 ± 0.172
1.029GlnTyr: 1.029 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
6.287ArgAla: 6.287 ± 0.65
1.257ArgCys: 1.257 ± 0.336
4.744ArgAsp: 4.744 ± 0.616
5.201ArgGlu: 5.201 ± 0.757
2.229ArgPhe: 2.229 ± 0.395
4.115ArgGly: 4.115 ± 0.515
1.257ArgHis: 1.257 ± 0.255
3.658ArgIle: 3.658 ± 0.5
2.115ArgLys: 2.115 ± 0.291
4.915ArgLeu: 4.915 ± 0.605
2.458ArgMet: 2.458 ± 0.384
2.0ArgAsn: 2.0 ± 0.418
3.258ArgPro: 3.258 ± 0.454
2.343ArgGln: 2.343 ± 0.34
4.972ArgArg: 4.972 ± 0.649
4.401ArgSer: 4.401 ± 0.466
3.143ArgThr: 3.143 ± 0.444
4.858ArgVal: 4.858 ± 0.675
1.829ArgTrp: 1.829 ± 0.322
2.057ArgTyr: 2.057 ± 0.318
0.0ArgXaa: 0.0 ± 0.0
Ser
5.944SerAla: 5.944 ± 0.684
0.457SerCys: 0.457 ± 0.202
3.658SerAsp: 3.658 ± 0.462
4.001SerGlu: 4.001 ± 0.518
2.458SerPhe: 2.458 ± 0.474
6.458SerGly: 6.458 ± 0.871
1.315SerHis: 1.315 ± 0.216
2.343SerIle: 2.343 ± 0.429
2.458SerLys: 2.458 ± 0.386
3.486SerLeu: 3.486 ± 0.508
1.429SerMet: 1.429 ± 0.248
2.286SerAsn: 2.286 ± 0.502
3.543SerPro: 3.543 ± 0.38
1.6SerGln: 1.6 ± 0.311
3.944SerArg: 3.944 ± 0.434
3.944SerSer: 3.944 ± 0.631
3.601SerThr: 3.601 ± 0.423
4.972SerVal: 4.972 ± 0.533
1.543SerTrp: 1.543 ± 0.312
1.143SerTyr: 1.143 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
6.115ThrAla: 6.115 ± 0.714
0.686ThrCys: 0.686 ± 0.21
3.715ThrAsp: 3.715 ± 0.53
3.601ThrGlu: 3.601 ± 0.384
1.943ThrPhe: 1.943 ± 0.339
5.772ThrGly: 5.772 ± 0.663
1.657ThrHis: 1.657 ± 0.287
3.601ThrIle: 3.601 ± 0.473
2.4ThrLys: 2.4 ± 0.399
5.029ThrLeu: 5.029 ± 0.557
0.857ThrMet: 0.857 ± 0.219
2.343ThrAsn: 2.343 ± 0.402
5.029ThrPro: 5.029 ± 0.748
1.829ThrGln: 1.829 ± 0.289
3.772ThrArg: 3.772 ± 0.452
3.944ThrSer: 3.944 ± 0.461
4.687ThrThr: 4.687 ± 0.566
5.83ThrVal: 5.83 ± 0.618
1.143ThrTrp: 1.143 ± 0.243
1.943ThrTyr: 1.943 ± 0.272
0.0ThrXaa: 0.0 ± 0.0
Val
7.316ValAla: 7.316 ± 0.694
1.2ValCys: 1.2 ± 0.27
5.029ValAsp: 5.029 ± 0.649
4.172ValGlu: 4.172 ± 0.569
2.172ValPhe: 2.172 ± 0.428
5.601ValGly: 5.601 ± 0.629
1.372ValHis: 1.372 ± 0.267
2.858ValIle: 2.858 ± 0.428
2.172ValLys: 2.172 ± 0.42
5.887ValLeu: 5.887 ± 0.65
1.429ValMet: 1.429 ± 0.224
2.458ValAsn: 2.458 ± 0.348
4.058ValPro: 4.058 ± 0.437
2.8ValGln: 2.8 ± 0.386
4.572ValArg: 4.572 ± 0.659
4.858ValSer: 4.858 ± 0.662
4.858ValThr: 4.858 ± 0.403
5.601ValVal: 5.601 ± 0.631
1.657ValTrp: 1.657 ± 0.383
1.372ValTyr: 1.372 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
2.057TrpAla: 2.057 ± 0.298
0.343TrpCys: 0.343 ± 0.14
1.6TrpAsp: 1.6 ± 0.33
1.086TrpGlu: 1.086 ± 0.242
0.686TrpPhe: 0.686 ± 0.19
0.857TrpGly: 0.857 ± 0.205
0.572TrpHis: 0.572 ± 0.202
1.257TrpIle: 1.257 ± 0.239
0.8TrpLys: 0.8 ± 0.188
2.0TrpLeu: 2.0 ± 0.328
0.8TrpMet: 0.8 ± 0.22
0.457TrpAsn: 0.457 ± 0.204
1.372TrpPro: 1.372 ± 0.303
1.086TrpGln: 1.086 ± 0.246
1.715TrpArg: 1.715 ± 0.275
1.315TrpSer: 1.315 ± 0.259
1.543TrpThr: 1.543 ± 0.275
1.715TrpVal: 1.715 ± 0.466
0.857TrpTrp: 0.857 ± 0.206
0.4TrpTyr: 0.4 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.229TyrAla: 2.229 ± 0.386
0.114TyrCys: 0.114 ± 0.081
2.0TyrAsp: 2.0 ± 0.379
1.715TyrGlu: 1.715 ± 0.328
0.914TyrPhe: 0.914 ± 0.218
2.057TyrGly: 2.057 ± 0.349
0.514TyrHis: 0.514 ± 0.17
1.086TyrIle: 1.086 ± 0.222
0.743TyrLys: 0.743 ± 0.205
2.0TyrLeu: 2.0 ± 0.375
0.343TyrMet: 0.343 ± 0.118
0.4TyrAsn: 0.4 ± 0.134
1.2TyrPro: 1.2 ± 0.253
0.572TyrGln: 0.572 ± 0.178
2.4TyrArg: 2.4 ± 0.43
1.257TyrSer: 1.257 ± 0.235
1.943TyrThr: 1.943 ± 0.334
2.458TyrVal: 2.458 ± 0.355
0.4TyrTrp: 0.4 ± 0.141
0.857TyrTyr: 0.857 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 106 proteins (17498 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski