Amino acid dipepetide frequency for Lactococcus phage c2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.145AlaAla: 0.145 ± 0.123
0.145AlaCys: 0.145 ± 0.146
3.479AlaAsp: 3.479 ± 0.592
5.943AlaGlu: 5.943 ± 0.976
2.464AlaPhe: 2.464 ± 0.572
3.479AlaGly: 3.479 ± 0.765
0.29AlaHis: 0.29 ± 0.187
4.348AlaIle: 4.348 ± 0.837
5.508AlaLys: 5.508 ± 0.863
6.088AlaLeu: 6.088 ± 1.313
1.449AlaMet: 1.449 ± 0.385
4.493AlaAsn: 4.493 ± 0.66
1.015AlaPro: 1.015 ± 0.424
2.899AlaGln: 2.899 ± 1.011
2.029AlaArg: 2.029 ± 0.573
3.189AlaSer: 3.189 ± 0.664
4.204AlaThr: 4.204 ± 1.048
3.914AlaVal: 3.914 ± 1.1
1.16AlaTrp: 1.16 ± 0.499
3.334AlaTyr: 3.334 ± 0.792
0.0AlaXaa: 0.0 ± 0.0
Cys
0.29CysAla: 0.29 ± 0.191
0.145CysCys: 0.145 ± 0.158
0.145CysAsp: 0.145 ± 0.179
0.29CysGlu: 0.29 ± 0.217
0.29CysPhe: 0.29 ± 0.244
0.87CysGly: 0.87 ± 0.35
0.58CysHis: 0.58 ± 0.535
0.145CysIle: 0.145 ± 0.143
0.725CysLys: 0.725 ± 0.386
1.015CysLeu: 1.015 ± 0.515
0.145CysMet: 0.145 ± 0.183
0.58CysAsn: 0.58 ± 0.38
0.29CysPro: 0.29 ± 0.192
0.29CysGln: 0.29 ± 0.231
0.58CysArg: 0.58 ± 0.315
0.725CysSer: 0.725 ± 0.298
0.725CysThr: 0.725 ± 0.32
0.145CysVal: 0.145 ± 0.135
0.29CysTrp: 0.29 ± 0.201
0.435CysTyr: 0.435 ± 0.24
0.0CysXaa: 0.0 ± 0.0
Asp
3.334AspAla: 3.334 ± 0.676
0.29AspCys: 0.29 ± 0.211
3.189AspAsp: 3.189 ± 0.752
5.218AspGlu: 5.218 ± 0.84
4.204AspPhe: 4.204 ± 0.786
5.653AspGly: 5.653 ± 1.551
0.0AspHis: 0.0 ± 0.0
3.914AspIle: 3.914 ± 1.057
5.218AspLys: 5.218 ± 0.822
4.928AspLeu: 4.928 ± 0.733
2.319AspMet: 2.319 ± 0.692
3.334AspAsn: 3.334 ± 0.669
1.16AspPro: 1.16 ± 0.283
0.87AspGln: 0.87 ± 0.321
1.449AspArg: 1.449 ± 0.417
3.334AspSer: 3.334 ± 0.576
3.914AspThr: 3.914 ± 0.77
2.319AspVal: 2.319 ± 0.684
1.449AspTrp: 1.449 ± 0.498
3.624AspTyr: 3.624 ± 0.905
0.0AspXaa: 0.0 ± 0.0
Glu
3.479GluAla: 3.479 ± 0.547
1.16GluCys: 1.16 ± 0.479
3.769GluAsp: 3.769 ± 0.715
6.088GluGlu: 6.088 ± 1.133
4.348GluPhe: 4.348 ± 0.892
3.044GluGly: 3.044 ± 0.562
1.594GluHis: 1.594 ± 0.515
4.348GluIle: 4.348 ± 0.633
4.638GluLys: 4.638 ± 0.872
6.523GluLeu: 6.523 ± 0.783
4.059GluMet: 4.059 ± 0.709
4.348GluAsn: 4.348 ± 0.853
1.015GluPro: 1.015 ± 0.366
4.204GluGln: 4.204 ± 0.901
4.059GluArg: 4.059 ± 0.847
2.609GluSer: 2.609 ± 0.57
5.218GluThr: 5.218 ± 0.821
5.508GluVal: 5.508 ± 0.851
0.87GluTrp: 0.87 ± 0.359
2.754GluTyr: 2.754 ± 0.872
0.0GluXaa: 0.0 ± 0.0
Phe
2.174PheAla: 2.174 ± 0.484
0.87PheCys: 0.87 ± 0.321
2.899PheAsp: 2.899 ± 0.569
2.899PheGlu: 2.899 ± 0.667
1.305PhePhe: 1.305 ± 0.494
3.334PheGly: 3.334 ± 0.564
0.435PheHis: 0.435 ± 0.212
4.928PheIle: 4.928 ± 1.057
5.218PheLys: 5.218 ± 1.195
2.174PheLeu: 2.174 ± 0.507
1.305PheMet: 1.305 ± 0.463
4.348PheAsn: 4.348 ± 0.663
1.305PhePro: 1.305 ± 0.503
0.58PheGln: 0.58 ± 0.224
2.029PheArg: 2.029 ± 0.409
2.754PheSer: 2.754 ± 0.595
2.899PheThr: 2.899 ± 0.744
2.754PheVal: 2.754 ± 0.537
0.58PheTrp: 0.58 ± 0.263
2.319PheTyr: 2.319 ± 0.581
0.0PheXaa: 0.0 ± 0.0
Gly
4.638GlyAla: 4.638 ± 1.07
0.29GlyCys: 0.29 ± 0.208
3.624GlyAsp: 3.624 ± 0.828
2.174GlyGlu: 2.174 ± 0.652
4.348GlyPhe: 4.348 ± 1.039
5.073GlyGly: 5.073 ± 0.717
0.87GlyHis: 0.87 ± 0.3
4.059GlyIle: 4.059 ± 0.866
5.363GlyLys: 5.363 ± 0.88
6.378GlyLeu: 6.378 ± 1.147
2.899GlyMet: 2.899 ± 0.707
3.914GlyAsn: 3.914 ± 0.691
0.58GlyPro: 0.58 ± 0.274
2.899GlyGln: 2.899 ± 0.712
2.464GlyArg: 2.464 ± 0.579
5.073GlySer: 5.073 ± 0.924
5.798GlyThr: 5.798 ± 1.025
5.218GlyVal: 5.218 ± 0.905
1.015GlyTrp: 1.015 ± 0.427
3.624GlyTyr: 3.624 ± 0.855
0.0GlyXaa: 0.0 ± 0.0
His
1.015HisAla: 1.015 ± 0.412
0.145HisCys: 0.145 ± 0.169
0.87HisAsp: 0.87 ± 0.348
0.58HisGlu: 0.58 ± 0.318
0.87HisPhe: 0.87 ± 0.404
0.29HisGly: 0.29 ± 0.159
0.145HisHis: 0.145 ± 0.169
1.015HisIle: 1.015 ± 0.326
1.015HisLys: 1.015 ± 0.392
1.449HisLeu: 1.449 ± 0.516
0.29HisMet: 0.29 ± 0.202
0.87HisAsn: 0.87 ± 0.476
0.145HisPro: 0.145 ± 0.147
0.435HisGln: 0.435 ± 0.276
0.29HisArg: 0.29 ± 0.19
0.725HisSer: 0.725 ± 0.312
0.58HisThr: 0.58 ± 0.279
0.58HisVal: 0.58 ± 0.315
0.435HisTrp: 0.435 ± 0.19
1.16HisTyr: 1.16 ± 0.439
0.0HisXaa: 0.0 ± 0.0
Ile
3.624IleAla: 3.624 ± 0.579
0.435IleCys: 0.435 ± 0.239
6.088IleAsp: 6.088 ± 0.803
6.523IleGlu: 6.523 ± 0.839
2.464IlePhe: 2.464 ± 0.615
4.928IleGly: 4.928 ± 0.661
0.87IleHis: 0.87 ± 0.364
4.638IleIle: 4.638 ± 0.851
5.653IleLys: 5.653 ± 1.025
4.204IleLeu: 4.204 ± 0.9
1.449IleMet: 1.449 ± 0.444
5.073IleAsn: 5.073 ± 0.761
2.029IlePro: 2.029 ± 0.39
2.029IleGln: 2.029 ± 0.49
2.029IleArg: 2.029 ± 0.592
2.609IleSer: 2.609 ± 0.528
4.059IleThr: 4.059 ± 0.615
2.754IleVal: 2.754 ± 0.57
0.725IleTrp: 0.725 ± 0.31
2.609IleTyr: 2.609 ± 0.49
0.0IleXaa: 0.0 ± 0.0
Lys
7.682LysAla: 7.682 ± 1.032
1.015LysCys: 1.015 ± 0.413
5.653LysAsp: 5.653 ± 1.135
8.117LysGlu: 8.117 ± 1.211
3.189LysPhe: 3.189 ± 0.659
6.813LysGly: 6.813 ± 0.959
1.305LysHis: 1.305 ± 0.444
4.493LysIle: 4.493 ± 0.97
7.537LysLys: 7.537 ± 1.199
6.668LysLeu: 6.668 ± 1.072
2.609LysMet: 2.609 ± 0.593
4.493LysAsn: 4.493 ± 0.74
2.609LysPro: 2.609 ± 0.604
4.059LysGln: 4.059 ± 0.571
3.334LysArg: 3.334 ± 0.801
3.624LysSer: 3.624 ± 0.597
3.479LysThr: 3.479 ± 0.835
6.378LysVal: 6.378 ± 1.362
1.449LysTrp: 1.449 ± 0.533
3.769LysTyr: 3.769 ± 0.903
0.0LysXaa: 0.0 ± 0.0
Leu
4.348LeuAla: 4.348 ± 1.071
1.015LeuCys: 1.015 ± 0.34
3.769LeuAsp: 3.769 ± 0.61
7.392LeuGlu: 7.392 ± 1.245
3.334LeuPhe: 3.334 ± 0.706
5.798LeuGly: 5.798 ± 1.517
1.594LeuHis: 1.594 ± 0.538
5.363LeuIle: 5.363 ± 1.151
7.392LeuLys: 7.392 ± 0.924
6.813LeuLeu: 6.813 ± 1.072
2.754LeuMet: 2.754 ± 1.083
5.943LeuAsn: 5.943 ± 1.014
2.029LeuPro: 2.029 ± 0.479
3.914LeuGln: 3.914 ± 0.821
2.029LeuArg: 2.029 ± 0.612
5.363LeuSer: 5.363 ± 0.832
4.783LeuThr: 4.783 ± 0.894
4.493LeuVal: 4.493 ± 0.738
0.725LeuTrp: 0.725 ± 0.36
3.189LeuTyr: 3.189 ± 0.63
0.0LeuXaa: 0.0 ± 0.0
Met
3.189MetAla: 3.189 ± 0.591
0.145MetCys: 0.145 ± 0.179
1.16MetAsp: 1.16 ± 0.391
2.174MetGlu: 2.174 ± 0.706
1.305MetPhe: 1.305 ± 0.511
1.449MetGly: 1.449 ± 0.412
0.0MetHis: 0.0 ± 0.0
2.754MetIle: 2.754 ± 0.736
3.334MetLys: 3.334 ± 0.53
2.464MetLeu: 2.464 ± 0.643
0.435MetMet: 0.435 ± 0.211
1.305MetAsn: 1.305 ± 0.439
0.87MetPro: 0.87 ± 0.386
1.015MetGln: 1.015 ± 0.36
0.725MetArg: 0.725 ± 0.252
1.884MetSer: 1.884 ± 0.424
1.739MetThr: 1.739 ± 0.581
1.594MetVal: 1.594 ± 0.427
0.29MetTrp: 0.29 ± 0.207
0.87MetTyr: 0.87 ± 0.371
0.0MetXaa: 0.0 ± 0.0
Asn
4.059AsnAla: 4.059 ± 0.965
0.725AsnCys: 0.725 ± 0.315
3.479AsnAsp: 3.479 ± 0.959
5.218AsnGlu: 5.218 ± 0.848
3.189AsnPhe: 3.189 ± 0.778
5.508AsnGly: 5.508 ± 0.873
0.435AsnHis: 0.435 ± 0.252
3.479AsnIle: 3.479 ± 0.849
6.233AsnLys: 6.233 ± 0.999
5.363AsnLeu: 5.363 ± 0.784
1.305AsnMet: 1.305 ± 0.399
4.204AsnAsn: 4.204 ± 0.974
2.754AsnPro: 2.754 ± 0.859
2.174AsnGln: 2.174 ± 0.567
2.029AsnArg: 2.029 ± 0.68
2.899AsnSer: 2.899 ± 0.537
4.493AsnThr: 4.493 ± 1.006
4.059AsnVal: 4.059 ± 0.591
1.015AsnTrp: 1.015 ± 0.395
2.754AsnTyr: 2.754 ± 0.552
0.0AsnXaa: 0.0 ± 0.0
Pro
0.87ProAla: 0.87 ± 0.392
0.0ProCys: 0.0 ± 0.0
1.739ProAsp: 1.739 ± 0.463
1.16ProGlu: 1.16 ± 0.341
1.449ProPhe: 1.449 ± 0.556
0.145ProGly: 0.145 ± 0.135
0.0ProHis: 0.0 ± 0.0
1.594ProIle: 1.594 ± 0.473
2.464ProLys: 2.464 ± 0.61
2.899ProLeu: 2.899 ± 0.438
0.29ProMet: 0.29 ± 0.208
2.029ProAsn: 2.029 ± 0.675
0.725ProPro: 0.725 ± 0.362
1.884ProGln: 1.884 ± 0.606
0.58ProArg: 0.58 ± 0.318
2.029ProSer: 2.029 ± 0.83
1.16ProThr: 1.16 ± 0.412
1.739ProVal: 1.739 ± 0.508
0.29ProTrp: 0.29 ± 0.223
1.16ProTyr: 1.16 ± 0.381
0.0ProXaa: 0.0 ± 0.0
Gln
3.334GlnAla: 3.334 ± 0.818
0.29GlnCys: 0.29 ± 0.212
1.739GlnAsp: 1.739 ± 0.611
1.594GlnGlu: 1.594 ± 0.397
1.594GlnPhe: 1.594 ± 0.511
3.044GlnGly: 3.044 ± 0.45
0.435GlnHis: 0.435 ± 0.227
2.899GlnIle: 2.899 ± 0.425
3.624GlnLys: 3.624 ± 0.855
2.754GlnLeu: 2.754 ± 0.477
1.884GlnMet: 1.884 ± 0.503
2.174GlnAsn: 2.174 ± 0.71
0.29GlnPro: 0.29 ± 0.168
2.029GlnGln: 2.029 ± 0.666
1.884GlnArg: 1.884 ± 0.577
2.464GlnSer: 2.464 ± 0.609
2.464GlnThr: 2.464 ± 0.402
1.739GlnVal: 1.739 ± 0.431
0.435GlnTrp: 0.435 ± 0.24
1.739GlnTyr: 1.739 ± 0.684
0.0GlnXaa: 0.0 ± 0.0
Arg
1.884ArgAla: 1.884 ± 0.473
0.29ArgCys: 0.29 ± 0.192
2.029ArgAsp: 2.029 ± 0.454
4.928ArgGlu: 4.928 ± 1.099
1.884ArgPhe: 1.884 ± 0.563
2.319ArgGly: 2.319 ± 0.546
0.435ArgHis: 0.435 ± 0.22
1.16ArgIle: 1.16 ± 0.471
3.479ArgLys: 3.479 ± 0.727
2.754ArgLeu: 2.754 ± 0.655
0.87ArgMet: 0.87 ± 0.344
2.029ArgAsn: 2.029 ± 0.661
1.16ArgPro: 1.16 ± 0.654
1.015ArgGln: 1.015 ± 0.332
1.16ArgArg: 1.16 ± 0.526
1.739ArgSer: 1.739 ± 0.627
2.029ArgThr: 2.029 ± 0.663
2.174ArgVal: 2.174 ± 0.713
0.725ArgTrp: 0.725 ± 0.379
1.16ArgTyr: 1.16 ± 0.461
0.0ArgXaa: 0.0 ± 0.0
Ser
3.189SerAla: 3.189 ± 0.682
0.0SerCys: 0.0 ± 0.0
3.624SerAsp: 3.624 ± 0.687
3.334SerGlu: 3.334 ± 0.86
2.609SerPhe: 2.609 ± 0.524
5.508SerGly: 5.508 ± 0.897
0.58SerHis: 0.58 ± 0.254
3.479SerIle: 3.479 ± 0.696
4.638SerLys: 4.638 ± 0.684
5.073SerLeu: 5.073 ± 0.75
1.015SerMet: 1.015 ± 0.372
3.479SerAsn: 3.479 ± 0.651
0.725SerPro: 0.725 ± 0.28
1.739SerGln: 1.739 ± 0.541
1.884SerArg: 1.884 ± 0.523
4.638SerSer: 4.638 ± 0.943
3.334SerThr: 3.334 ± 0.649
3.769SerVal: 3.769 ± 0.586
1.015SerTrp: 1.015 ± 0.464
3.479SerTyr: 3.479 ± 0.91
0.0SerXaa: 0.0 ± 0.0
Thr
3.479ThrAla: 3.479 ± 0.623
0.145ThrCys: 0.145 ± 0.152
5.218ThrAsp: 5.218 ± 1.01
3.479ThrGlu: 3.479 ± 0.927
2.754ThrPhe: 2.754 ± 0.729
5.363ThrGly: 5.363 ± 1.208
0.87ThrHis: 0.87 ± 0.363
5.218ThrIle: 5.218 ± 0.759
5.653ThrLys: 5.653 ± 0.832
6.668ThrLeu: 6.668 ± 0.897
0.87ThrMet: 0.87 ± 0.368
2.754ThrAsn: 2.754 ± 0.514
1.884ThrPro: 1.884 ± 0.509
2.319ThrGln: 2.319 ± 0.569
1.449ThrArg: 1.449 ± 0.412
3.914ThrSer: 3.914 ± 1.138
3.044ThrThr: 3.044 ± 0.586
4.348ThrVal: 4.348 ± 0.76
1.015ThrTrp: 1.015 ± 0.466
2.464ThrTyr: 2.464 ± 0.691
0.0ThrXaa: 0.0 ± 0.0
Val
3.044ValAla: 3.044 ± 0.63
0.29ValCys: 0.29 ± 0.207
4.059ValAsp: 4.059 ± 0.693
3.479ValGlu: 3.479 ± 0.724
2.464ValPhe: 2.464 ± 0.548
2.609ValGly: 2.609 ± 0.923
1.015ValHis: 1.015 ± 0.356
3.769ValIle: 3.769 ± 0.775
5.798ValLys: 5.798 ± 0.793
3.479ValLeu: 3.479 ± 0.75
1.594ValMet: 1.594 ± 0.549
4.204ValAsn: 4.204 ± 0.93
2.319ValPro: 2.319 ± 0.65
2.174ValGln: 2.174 ± 0.691
3.334ValArg: 3.334 ± 0.589
4.928ValSer: 4.928 ± 1.098
4.204ValThr: 4.204 ± 0.683
2.609ValVal: 2.609 ± 0.716
1.449ValTrp: 1.449 ± 0.362
2.464ValTyr: 2.464 ± 0.622
0.0ValXaa: 0.0 ± 0.0
Trp
2.029TrpAla: 2.029 ± 0.607
0.435TrpCys: 0.435 ± 0.269
0.725TrpAsp: 0.725 ± 0.297
0.58TrpGlu: 0.58 ± 0.32
1.015TrpPhe: 1.015 ± 0.409
1.16TrpGly: 1.16 ± 0.342
0.29TrpHis: 0.29 ± 0.2
0.58TrpIle: 0.58 ± 0.285
1.015TrpLys: 1.015 ± 0.379
2.174TrpLeu: 2.174 ± 0.606
0.29TrpMet: 0.29 ± 0.211
1.305TrpAsn: 1.305 ± 0.618
0.0TrpPro: 0.0 ± 0.0
0.435TrpGln: 0.435 ± 0.319
0.58TrpArg: 0.58 ± 0.328
0.58TrpSer: 0.58 ± 0.35
1.305TrpThr: 1.305 ± 0.506
0.87TrpVal: 0.87 ± 0.386
0.29TrpTrp: 0.29 ± 0.172
0.435TrpTyr: 0.435 ± 0.27
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.769TyrAla: 3.769 ± 0.502
0.87TyrCys: 0.87 ± 0.356
2.464TyrAsp: 2.464 ± 0.563
2.464TyrGlu: 2.464 ± 0.621
2.029TyrPhe: 2.029 ± 0.591
3.914TyrGly: 3.914 ± 0.685
1.16TyrHis: 1.16 ± 0.475
2.609TyrIle: 2.609 ± 0.811
3.769TyrLys: 3.769 ± 0.63
2.174TyrLeu: 2.174 ± 0.663
0.725TyrMet: 0.725 ± 0.322
4.493TyrAsn: 4.493 ± 0.871
1.305TyrPro: 1.305 ± 0.514
1.449TyrGln: 1.449 ± 0.335
1.305TyrArg: 1.305 ± 0.437
2.174TyrSer: 2.174 ± 0.657
3.479TyrThr: 3.479 ± 1.019
2.319TyrVal: 2.319 ± 0.658
0.87TyrTrp: 0.87 ± 0.352
2.319TyrTyr: 2.319 ± 0.807
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 39 proteins (6900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski