Vibrio phage Ares1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7QIC6|A0A2I7QIC6_9CAUD Uncharacterized protein OS=Vibrio phage Ares1 OX=2070058 GN=Ares1_0092 PE=4 SV=1
MM1 pKa = 7.66YY2 pKa = 9.96DD3 pKa = 3.41RR4 pKa = 11.84NDD6 pKa = 3.08EE7 pKa = 4.07RR8 pKa = 11.84FYY10 pKa = 10.73IQPISYY16 pKa = 10.1LNTEE20 pKa = 4.23GGVSYY25 pKa = 10.82EE26 pKa = 3.85AMTAEE31 pKa = 5.29FGDD34 pKa = 3.68GLNRR38 pKa = 11.84INTTLFEE45 pKa = 4.93DD46 pKa = 3.72KK47 pKa = 11.01VGILIFRR54 pKa = 11.84NTEE57 pKa = 4.05LEE59 pKa = 4.21PGLALDD65 pKa = 4.01IEE67 pKa = 4.88KK68 pKa = 10.81GDD70 pKa = 3.7TVPNPKK76 pKa = 9.85PGEE79 pKa = 3.8NAIALNFHH87 pKa = 6.24TLQSIDD93 pKa = 3.37AAIGRR98 pKa = 11.84LQYY101 pKa = 10.14IRR103 pKa = 11.84EE104 pKa = 4.04VFIEE108 pKa = 4.07RR109 pKa = 11.84EE110 pKa = 3.77EE111 pKa = 3.9AAKK114 pKa = 10.35KK115 pKa = 10.48GKK117 pKa = 10.41APEE120 pKa = 5.02AGDD123 pKa = 3.83DD124 pKa = 5.05CPTEE128 pKa = 4.18DD129 pKa = 4.4CEE131 pKa = 6.19GILEE135 pKa = 4.95HH136 pKa = 7.36YY137 pKa = 10.35PDD139 pKa = 5.02DD140 pKa = 4.33CQCAVVPPPCAACTNAPLSCCVCGWSEE167 pKa = 3.81EE168 pKa = 4.38DD169 pKa = 3.43EE170 pKa = 4.36

Molecular weight: 18.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7QII9|A0A2I7QII9_9CAUD Uncharacterized protein OS=Vibrio phage Ares1 OX=2070058 GN=Ares1_0108 PE=4 SV=1
MM1 pKa = 7.77DD2 pKa = 3.32QQLILGLSVLTAFILIVRR20 pKa = 11.84AVVVGFSIKK29 pKa = 10.2LNRR32 pKa = 11.84YY33 pKa = 8.79RR34 pKa = 11.84LEE36 pKa = 4.05LRR38 pKa = 11.84LKK40 pKa = 10.54HH41 pKa = 5.94LAPLAAFSGNCNSLLFSRR59 pKa = 11.84YY60 pKa = 8.86IIRR63 pKa = 11.84VSEE66 pKa = 3.88AANAEE71 pKa = 4.16DD72 pKa = 3.99FTRR75 pKa = 11.84ANALLDD81 pKa = 3.53RR82 pKa = 11.84LEE84 pKa = 4.15RR85 pKa = 11.84RR86 pKa = 11.84VRR88 pKa = 11.84AA89 pKa = 3.71

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

119

0

119

24983

40

1379

209.9

23.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.262 ± 0.34

1.277 ± 0.127

6.18 ± 0.144

6.841 ± 0.249

3.622 ± 0.139

6.196 ± 0.193

2.057 ± 0.123

6.324 ± 0.197

6.733 ± 0.308

9.138 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.122

5.148 ± 0.145

4.015 ± 0.184

3.887 ± 0.192

5.204 ± 0.178

5.948 ± 0.192

6.408 ± 0.245

6.444 ± 0.185

0.777 ± 0.07

3.314 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski