Streptococcus satellite phage Javan286

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZNC7|A0A4D5ZNC7_9VIRU Bro-N domain-containing protein OS=Streptococcus satellite phage Javan286 OX=2558608 GN=JavanS286_0003 PE=4 SV=1
MM1 pKa = 7.64LYY3 pKa = 9.76IEE5 pKa = 4.24QLEE8 pKa = 4.2EE9 pKa = 4.19EE10 pKa = 4.48IKK12 pKa = 10.9EE13 pKa = 4.06EE14 pKa = 3.98QQNSDD19 pKa = 3.7KK20 pKa = 10.9QPKK23 pKa = 8.11TMNEE27 pKa = 4.16LYY29 pKa = 10.56NLPGKK34 pKa = 9.9QEE36 pKa = 4.07GFDD39 pKa = 4.04GIAFLVFNCLGSFLMMLEE57 pKa = 4.6DD58 pKa = 6.32DD59 pKa = 4.91EE60 pKa = 5.77LHH62 pKa = 6.35CQPKK66 pKa = 10.06PIEE69 pKa = 4.38EE70 pKa = 4.85DD71 pKa = 3.12SDD73 pKa = 4.3LDD75 pKa = 3.72NEE77 pKa = 4.42ANKK80 pKa = 9.15TLAYY84 pKa = 10.5DD85 pKa = 3.95EE86 pKa = 4.69LVKK89 pKa = 10.78KK90 pKa = 10.67

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZI12|A0A4D5ZI12_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan286 OX=2558608 GN=JavanS286_0008 PE=4 SV=1
MM1 pKa = 7.05QNYY4 pKa = 6.87YY5 pKa = 8.99TPKK8 pKa = 10.23SKK10 pKa = 10.53HH11 pKa = 4.74LTLTEE16 pKa = 3.38RR17 pKa = 11.84RR18 pKa = 11.84MIEE21 pKa = 3.44RR22 pKa = 11.84WLQEE26 pKa = 3.71GLSNRR31 pKa = 11.84EE32 pKa = 3.29IARR35 pKa = 11.84RR36 pKa = 11.84LAKK39 pKa = 10.4APQTIHH45 pKa = 6.92NEE47 pKa = 4.04VKK49 pKa = 10.29RR50 pKa = 11.84GQVRR54 pKa = 11.84QQVRR58 pKa = 11.84KK59 pKa = 9.96GKK61 pKa = 10.81FEE63 pKa = 4.47VIYY66 pKa = 10.92SADD69 pKa = 3.74FAQKK73 pKa = 10.51AYY75 pKa = 10.43QNNRR79 pKa = 11.84KK80 pKa = 9.53RR81 pKa = 11.84SVKK84 pKa = 9.81QASLTKK90 pKa = 10.17GLKK93 pKa = 9.87EE94 pKa = 4.48KK95 pKa = 8.91ITHH98 pKa = 6.22YY99 pKa = 10.4IEE101 pKa = 4.27QKK103 pKa = 10.13YY104 pKa = 9.26SPEE107 pKa = 3.8MMVKK111 pKa = 10.16SKK113 pKa = 10.84GIPVPISTIYY123 pKa = 9.16YY124 pKa = 7.43WIHH127 pKa = 6.45HH128 pKa = 5.8GHH130 pKa = 6.98LGWTKK135 pKa = 11.34ADD137 pKa = 2.94ILYY140 pKa = 9.32PRR142 pKa = 11.84QEE144 pKa = 4.01KK145 pKa = 10.4AKK147 pKa = 10.11KK148 pKa = 9.6KK149 pKa = 9.53HH150 pKa = 5.94ASPNFKK156 pKa = 9.98PAGKK160 pKa = 9.89SIEE163 pKa = 4.1EE164 pKa = 4.07RR165 pKa = 11.84PEE167 pKa = 4.35SINKK171 pKa = 9.57RR172 pKa = 11.84EE173 pKa = 4.05NIGDD177 pKa = 3.79FEE179 pKa = 6.17IDD181 pKa = 3.31TVIQTRR187 pKa = 11.84AKK189 pKa = 10.38NEE191 pKa = 4.05CLLTLTDD198 pKa = 3.41RR199 pKa = 11.84KK200 pKa = 10.41SRR202 pKa = 11.84YY203 pKa = 9.43QIIRR207 pKa = 11.84LIPDD211 pKa = 3.59KK212 pKa = 11.17SAFSVNQALKK222 pKa = 10.72AILKK226 pKa = 9.3DD227 pKa = 3.54YY228 pKa = 11.14QMNSITADD236 pKa = 3.12NGVEE240 pKa = 4.25FSRR243 pKa = 11.84LAEE246 pKa = 4.26VFNPTHH252 pKa = 6.94IYY254 pKa = 9.03YY255 pKa = 10.46AHH257 pKa = 7.67PYY259 pKa = 9.47SSWEE263 pKa = 3.69RR264 pKa = 11.84GTNEE268 pKa = 3.29NHH270 pKa = 5.89NRR272 pKa = 11.84LIRR275 pKa = 11.84RR276 pKa = 11.84WLPKK280 pKa = 9.64GSKK283 pKa = 9.91NATQQQVAFIEE294 pKa = 4.16NWINNYY300 pKa = 8.73PKK302 pKa = 10.82KK303 pKa = 10.49LFNYY307 pKa = 9.38KK308 pKa = 9.99SPKK311 pKa = 10.06EE312 pKa = 3.87FLQAGG317 pKa = 3.78

Molecular weight: 37.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2658

46

389

177.2

20.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.966 ± 0.403

1.129 ± 0.333

3.913 ± 0.579

7.111 ± 0.997

4.74 ± 0.755

4.552 ± 0.564

2.972 ± 0.417

7.788 ± 0.674

9.255 ± 0.724

11.249 ± 0.933

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.34

5.944 ± 0.371

3.01 ± 0.353

4.176 ± 0.381

4.853 ± 0.565

5.982 ± 0.436

5.38 ± 0.58

4.816 ± 0.351

0.941 ± 0.154

4.703 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski