Amino acid dipepetide frequency for alpha proteobacterium IMCC14465

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.447AlaAla: 10.447 ± 0.179
1.114AlaCys: 1.114 ± 0.043
6.174AlaAsp: 6.174 ± 0.107
6.622AlaGlu: 6.622 ± 0.124
3.541AlaPhe: 3.541 ± 0.084
8.005AlaGly: 8.005 ± 0.124
2.206AlaHis: 2.206 ± 0.068
5.404AlaIle: 5.404 ± 0.108
4.302AlaLys: 4.302 ± 0.103
10.327AlaLeu: 10.327 ± 0.153
2.813AlaMet: 2.813 ± 0.071
3.03AlaAsn: 3.03 ± 0.086
3.795AlaPro: 3.795 ± 0.091
3.756AlaGln: 3.756 ± 0.078
5.352AlaArg: 5.352 ± 0.102
5.716AlaSer: 5.716 ± 0.097
4.617AlaThr: 4.617 ± 0.098
6.082AlaVal: 6.082 ± 0.117
0.834AlaTrp: 0.834 ± 0.043
2.572AlaTyr: 2.572 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.98CysAla: 0.98 ± 0.045
0.1CysCys: 0.1 ± 0.012
0.695CysAsp: 0.695 ± 0.033
0.5CysGlu: 0.5 ± 0.029
0.395CysPhe: 0.395 ± 0.027
0.907CysGly: 0.907 ± 0.047
0.265CysHis: 0.265 ± 0.022
0.485CysIle: 0.485 ± 0.034
0.324CysLys: 0.324 ± 0.02
0.99CysLeu: 0.99 ± 0.043
0.18CysMet: 0.18 ± 0.016
0.287CysAsn: 0.287 ± 0.022
0.505CysPro: 0.505 ± 0.03
0.31CysGln: 0.31 ± 0.024
0.483CysArg: 0.483 ± 0.029
0.531CysSer: 0.531 ± 0.035
0.419CysThr: 0.419 ± 0.029
0.643CysVal: 0.643 ± 0.031
0.124CysTrp: 0.124 ± 0.015
0.251CysTyr: 0.251 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
6.054AspAla: 6.054 ± 0.107
0.582AspCys: 0.582 ± 0.037
3.864AspAsp: 3.864 ± 0.103
4.287AspGlu: 4.287 ± 0.112
2.8AspPhe: 2.8 ± 0.073
4.743AspGly: 4.743 ± 0.113
1.07AspHis: 1.07 ± 0.041
4.997AspIle: 4.997 ± 0.095
3.285AspLys: 3.285 ± 0.079
5.636AspLeu: 5.636 ± 0.103
2.245AspMet: 2.245 ± 0.064
2.598AspAsn: 2.598 ± 0.064
2.589AspPro: 2.589 ± 0.073
1.529AspGln: 1.529 ± 0.049
3.0AspArg: 3.0 ± 0.077
2.791AspSer: 2.791 ± 0.076
3.34AspThr: 3.34 ± 0.082
4.534AspVal: 4.534 ± 0.098
0.904AspTrp: 0.904 ± 0.037
2.159AspTyr: 2.159 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
7.174GluAla: 7.174 ± 0.143
0.366GluCys: 0.366 ± 0.028
3.808GluAsp: 3.808 ± 0.095
4.141GluGlu: 4.141 ± 0.11
2.096GluPhe: 2.096 ± 0.063
4.209GluGly: 4.209 ± 0.104
1.107GluHis: 1.107 ± 0.047
4.7GluIle: 4.7 ± 0.083
4.222GluLys: 4.222 ± 0.088
5.462GluLeu: 5.462 ± 0.099
1.942GluMet: 1.942 ± 0.057
3.361GluAsn: 3.361 ± 0.088
1.967GluPro: 1.967 ± 0.055
2.313GluGln: 2.313 ± 0.066
3.395GluArg: 3.395 ± 0.076
2.85GluSer: 2.85 ± 0.061
4.699GluThr: 4.699 ± 0.099
3.897GluVal: 3.897 ± 0.092
0.649GluTrp: 0.649 ± 0.034
1.206GluTyr: 1.206 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.722PheAla: 3.722 ± 0.091
0.47PheCys: 0.47 ± 0.028
2.908PheAsp: 2.908 ± 0.077
2.644PheGlu: 2.644 ± 0.074
1.848PhePhe: 1.848 ± 0.07
3.363PheGly: 3.363 ± 0.082
0.795PheHis: 0.795 ± 0.033
2.555PheIle: 2.555 ± 0.075
1.857PheLys: 1.857 ± 0.056
3.795PheLeu: 3.795 ± 0.102
1.175PheMet: 1.175 ± 0.048
1.726PheAsn: 1.726 ± 0.056
1.518PhePro: 1.518 ± 0.055
1.133PheGln: 1.133 ± 0.043
1.757PheArg: 1.757 ± 0.057
3.0PheSer: 3.0 ± 0.08
2.352PheThr: 2.352 ± 0.071
2.62PheVal: 2.62 ± 0.071
0.524PheTrp: 0.524 ± 0.03
1.211PheTyr: 1.211 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
6.696GlyAla: 6.696 ± 0.132
0.77GlyCys: 0.77 ± 0.037
4.602GlyAsp: 4.602 ± 0.104
4.487GlyGlu: 4.487 ± 0.079
3.808GlyPhe: 3.808 ± 0.083
6.079GlyGly: 6.079 ± 0.127
1.789GlyHis: 1.789 ± 0.055
4.678GlyIle: 4.678 ± 0.102
3.771GlyLys: 3.771 ± 0.083
8.182GlyLeu: 8.182 ± 0.127
2.237GlyMet: 2.237 ± 0.078
2.53GlyAsn: 2.53 ± 0.075
2.452GlyPro: 2.452 ± 0.07
3.03GlyGln: 3.03 ± 0.067
4.148GlyArg: 4.148 ± 0.086
4.132GlySer: 4.132 ± 0.088
3.91GlyThr: 3.91 ± 0.085
5.613GlyVal: 5.613 ± 0.106
1.041GlyTrp: 1.041 ± 0.04
2.34GlyTyr: 2.34 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
2.042HisAla: 2.042 ± 0.059
0.261HisCys: 0.261 ± 0.02
1.189HisAsp: 1.189 ± 0.047
1.241HisGlu: 1.241 ± 0.045
0.912HisPhe: 0.912 ± 0.033
1.57HisGly: 1.57 ± 0.051
0.614HisHis: 0.614 ± 0.041
1.453HisIle: 1.453 ± 0.047
0.894HisLys: 0.894 ± 0.042
2.137HisLeu: 2.137 ± 0.067
0.548HisMet: 0.548 ± 0.033
0.85HisAsn: 0.85 ± 0.041
1.304HisPro: 1.304 ± 0.044
0.797HisGln: 0.797 ± 0.04
1.019HisArg: 1.019 ± 0.042
0.98HisSer: 0.98 ± 0.039
0.797HisThr: 0.797 ± 0.038
1.309HisVal: 1.309 ± 0.049
0.231HisTrp: 0.231 ± 0.02
0.656HisTyr: 0.656 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.576IleAla: 6.576 ± 0.122
0.841IleCys: 0.841 ± 0.037
4.448IleAsp: 4.448 ± 0.083
4.28IleGlu: 4.28 ± 0.085
2.594IlePhe: 2.594 ± 0.091
5.284IleGly: 5.284 ± 0.103
1.178IleHis: 1.178 ± 0.046
4.243IleIle: 4.243 ± 0.097
2.762IleLys: 2.762 ± 0.072
5.604IleLeu: 5.604 ± 0.106
1.585IleMet: 1.585 ± 0.052
2.584IleAsn: 2.584 ± 0.077
2.491IlePro: 2.491 ± 0.057
1.809IleGln: 1.809 ± 0.063
3.239IleArg: 3.239 ± 0.075
4.497IleSer: 4.497 ± 0.097
3.519IleThr: 3.519 ± 0.079
4.059IleVal: 4.059 ± 0.084
0.666IleTrp: 0.666 ± 0.039
1.689IleTyr: 1.689 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
4.432LysAla: 4.432 ± 0.091
0.314LysCys: 0.314 ± 0.024
2.62LysAsp: 2.62 ± 0.072
2.613LysGlu: 2.613 ± 0.075
1.68LysPhe: 1.68 ± 0.057
3.1LysGly: 3.1 ± 0.087
0.953LysHis: 0.953 ± 0.044
3.508LysIle: 3.508 ± 0.073
2.974LysLys: 2.974 ± 0.091
4.729LysLeu: 4.729 ± 0.089
1.373LysMet: 1.373 ± 0.047
2.423LysAsn: 2.423 ± 0.071
2.348LysPro: 2.348 ± 0.065
1.791LysGln: 1.791 ± 0.061
2.647LysArg: 2.647 ± 0.078
3.188LysSer: 3.188 ± 0.065
3.083LysThr: 3.083 ± 0.073
2.842LysVal: 2.842 ± 0.079
0.466LysTrp: 0.466 ± 0.028
1.131LysTyr: 1.131 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
9.718LeuAla: 9.718 ± 0.158
0.926LeuCys: 0.926 ± 0.044
6.081LeuAsp: 6.081 ± 0.108
6.269LeuGlu: 6.269 ± 0.117
3.69LeuPhe: 3.69 ± 0.094
7.178LeuGly: 7.178 ± 0.121
1.811LeuHis: 1.811 ± 0.064
5.838LeuIle: 5.838 ± 0.118
5.048LeuLys: 5.048 ± 0.11
8.507LeuLeu: 8.507 ± 0.186
2.808LeuMet: 2.808 ± 0.077
4.005LeuAsn: 4.005 ± 0.087
5.063LeuPro: 5.063 ± 0.102
3.168LeuGln: 3.168 ± 0.074
4.928LeuArg: 4.928 ± 0.102
7.585LeuSer: 7.585 ± 0.13
6.145LeuThr: 6.145 ± 0.111
6.396LeuVal: 6.396 ± 0.108
0.914LeuTrp: 0.914 ± 0.038
2.131LeuTyr: 2.131 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.881MetAla: 2.881 ± 0.07
0.185MetCys: 0.185 ± 0.016
1.565MetAsp: 1.565 ± 0.049
1.392MetGlu: 1.392 ± 0.057
0.909MetPhe: 0.909 ± 0.045
2.001MetGly: 2.001 ± 0.057
0.558MetHis: 0.558 ± 0.029
1.697MetIle: 1.697 ± 0.057
1.301MetLys: 1.301 ± 0.049
2.681MetLeu: 2.681 ± 0.062
0.889MetMet: 0.889 ± 0.041
1.087MetAsn: 1.087 ± 0.045
1.635MetPro: 1.635 ± 0.051
1.153MetGln: 1.153 ± 0.047
1.648MetArg: 1.648 ± 0.059
2.254MetSer: 2.254 ± 0.063
2.118MetThr: 2.118 ± 0.06
1.777MetVal: 1.777 ± 0.063
0.217MetTrp: 0.217 ± 0.018
0.359MetTyr: 0.359 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.591AsnAla: 3.591 ± 0.093
0.382AsnCys: 0.382 ± 0.027
2.189AsnAsp: 2.189 ± 0.067
2.194AsnGlu: 2.194 ± 0.074
1.765AsnPhe: 1.765 ± 0.059
2.942AsnGly: 2.942 ± 0.085
0.792AsnHis: 0.792 ± 0.036
2.906AsnIle: 2.906 ± 0.082
1.872AsnLys: 1.872 ± 0.052
4.105AsnLeu: 4.105 ± 0.082
1.167AsnMet: 1.167 ± 0.047
1.68AsnAsn: 1.68 ± 0.062
2.231AsnPro: 2.231 ± 0.063
1.402AsnGln: 1.402 ± 0.055
2.148AsnArg: 2.148 ± 0.063
2.087AsnSer: 2.087 ± 0.063
1.86AsnThr: 1.86 ± 0.056
2.542AsnVal: 2.542 ± 0.063
0.587AsnTrp: 0.587 ± 0.031
1.073AsnTyr: 1.073 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
3.987ProAla: 3.987 ± 0.076
0.336ProCys: 0.336 ± 0.021
3.668ProAsp: 3.668 ± 0.095
3.971ProGlu: 3.971 ± 0.083
1.889ProPhe: 1.889 ± 0.057
3.213ProGly: 3.213 ± 0.088
1.002ProHis: 1.002 ± 0.046
2.52ProIle: 2.52 ± 0.065
1.84ProLys: 1.84 ± 0.051
4.003ProLeu: 4.003 ± 0.077
1.034ProMet: 1.034 ± 0.044
1.626ProAsn: 1.626 ± 0.048
1.908ProPro: 1.908 ± 0.067
1.538ProGln: 1.538 ± 0.051
1.897ProArg: 1.897 ± 0.059
2.699ProSer: 2.699 ± 0.065
2.062ProThr: 2.062 ± 0.068
3.457ProVal: 3.457 ± 0.084
0.422ProTrp: 0.422 ± 0.03
1.177ProTyr: 1.177 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
3.919GlnAla: 3.919 ± 0.086
0.251GlnCys: 0.251 ± 0.021
1.974GlnAsp: 1.974 ± 0.063
2.108GlnGlu: 2.108 ± 0.066
1.28GlnPhe: 1.28 ± 0.043
2.387GlnGly: 2.387 ± 0.063
0.677GlnHis: 0.677 ± 0.032
2.722GlnIle: 2.722 ± 0.074
2.045GlnLys: 2.045 ± 0.063
3.088GlnLeu: 3.088 ± 0.07
0.963GlnMet: 0.963 ± 0.04
1.838GlnAsn: 1.838 ± 0.057
1.443GlnPro: 1.443 ± 0.049
1.387GlnGln: 1.387 ± 0.053
1.78GlnArg: 1.78 ± 0.062
2.404GlnSer: 2.404 ± 0.068
2.174GlnThr: 2.174 ± 0.06
2.111GlnVal: 2.111 ± 0.058
0.368GlnTrp: 0.368 ± 0.028
0.716GlnTyr: 0.716 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
4.505ArgAla: 4.505 ± 0.092
0.41ArgCys: 0.41 ± 0.026
3.364ArgAsp: 3.364 ± 0.084
3.151ArgGlu: 3.151 ± 0.078
2.365ArgPhe: 2.365 ± 0.063
3.446ArgGly: 3.446 ± 0.075
1.329ArgHis: 1.329 ± 0.046
3.164ArgIle: 3.164 ± 0.067
2.484ArgLys: 2.484 ± 0.082
6.225ArgLeu: 6.225 ± 0.121
1.535ArgMet: 1.535 ± 0.045
1.936ArgAsn: 1.936 ± 0.062
2.309ArgPro: 2.309 ± 0.062
2.25ArgGln: 2.25 ± 0.074
3.218ArgArg: 3.218 ± 0.099
2.657ArgSer: 2.657 ± 0.079
2.147ArgThr: 2.147 ± 0.07
3.58ArgVal: 3.58 ± 0.093
0.546ArgTrp: 0.546 ± 0.033
1.482ArgTyr: 1.482 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
5.538SerAla: 5.538 ± 0.101
0.548SerCys: 0.548 ± 0.032
4.019SerAsp: 4.019 ± 0.081
3.998SerGlu: 3.998 ± 0.086
2.878SerPhe: 2.878 ± 0.07
5.662SerGly: 5.662 ± 0.127
1.25SerHis: 1.25 ± 0.049
3.493SerIle: 3.493 ± 0.081
2.62SerLys: 2.62 ± 0.067
6.444SerLeu: 6.444 ± 0.128
1.599SerMet: 1.599 ± 0.05
2.082SerAsn: 2.082 ± 0.072
2.883SerPro: 2.883 ± 0.069
2.225SerGln: 2.225 ± 0.062
3.183SerArg: 3.183 ± 0.089
3.858SerSer: 3.858 ± 0.109
2.769SerThr: 2.769 ± 0.075
4.097SerVal: 4.097 ± 0.076
0.736SerTrp: 0.736 ± 0.035
1.74SerTyr: 1.74 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
5.338ThrAla: 5.338 ± 0.122
0.46ThrCys: 0.46 ± 0.032
3.549ThrAsp: 3.549 ± 0.087
3.196ThrGlu: 3.196 ± 0.08
1.899ThrPhe: 1.899 ± 0.057
4.965ThrGly: 4.965 ± 0.092
1.324ThrHis: 1.324 ± 0.046
3.173ThrIle: 3.173 ± 0.076
2.157ThrLys: 2.157 ± 0.069
5.818ThrLeu: 5.818 ± 0.095
1.212ThrMet: 1.212 ± 0.044
1.938ThrAsn: 1.938 ± 0.065
3.207ThrPro: 3.207 ± 0.082
2.133ThrGln: 2.133 ± 0.076
2.701ThrArg: 2.701 ± 0.064
3.495ThrSer: 3.495 ± 0.089
2.818ThrThr: 2.818 ± 0.085
3.627ThrVal: 3.627 ± 0.093
0.378ThrTrp: 0.378 ± 0.026
1.457ThrTyr: 1.457 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
5.984ValAla: 5.984 ± 0.104
0.619ValCys: 0.619 ± 0.032
3.973ValAsp: 3.973 ± 0.084
4.117ValGlu: 4.117 ± 0.089
2.869ValPhe: 2.869 ± 0.068
4.421ValGly: 4.421 ± 0.098
1.18ValHis: 1.18 ± 0.041
4.407ValIle: 4.407 ± 0.093
2.954ValLys: 2.954 ± 0.071
6.362ValLeu: 6.362 ± 0.12
1.994ValMet: 1.994 ± 0.059
2.694ValAsn: 2.694 ± 0.067
2.957ValPro: 2.957 ± 0.074
2.181ValGln: 2.181 ± 0.06
3.279ValArg: 3.279 ± 0.089
4.853ValSer: 4.853 ± 0.105
4.371ValThr: 4.371 ± 0.091
4.344ValVal: 4.344 ± 0.096
0.717ValTrp: 0.717 ± 0.035
1.58ValTyr: 1.58 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.794TrpAla: 0.794 ± 0.037
0.122TrpCys: 0.122 ± 0.014
0.577TrpAsp: 0.577 ± 0.035
0.505TrpGlu: 0.505 ± 0.027
0.5TrpPhe: 0.5 ± 0.035
0.76TrpGly: 0.76 ± 0.034
0.285TrpHis: 0.285 ± 0.02
0.585TrpIle: 0.585 ± 0.032
0.482TrpLys: 0.482 ± 0.024
1.375TrpLeu: 1.375 ± 0.056
0.346TrpMet: 0.346 ± 0.023
0.315TrpAsn: 0.315 ± 0.023
0.539TrpPro: 0.539 ± 0.033
0.629TrpGln: 0.629 ± 0.036
0.744TrpArg: 0.744 ± 0.031
0.675TrpSer: 0.675 ± 0.032
0.483TrpThr: 0.483 ± 0.031
0.673TrpVal: 0.673 ± 0.032
0.171TrpTrp: 0.171 ± 0.016
0.278TrpTyr: 0.278 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.332TyrAla: 2.332 ± 0.065
0.319TyrCys: 0.319 ± 0.023
1.74TyrAsp: 1.74 ± 0.06
1.848TyrGlu: 1.848 ± 0.052
1.251TyrPhe: 1.251 ± 0.054
2.062TyrGly: 2.062 ± 0.064
0.617TyrHis: 0.617 ± 0.032
1.419TyrIle: 1.419 ± 0.053
1.024TyrLys: 1.024 ± 0.04
2.738TyrLeu: 2.738 ± 0.07
0.66TyrMet: 0.66 ± 0.037
0.926TyrAsn: 0.926 ± 0.043
1.106TyrPro: 1.106 ± 0.051
1.1TyrGln: 1.1 ± 0.045
1.538TyrArg: 1.538 ± 0.045
1.368TyrSer: 1.368 ± 0.063
1.162TyrThr: 1.162 ± 0.048
1.655TyrVal: 1.655 ± 0.055
0.343TyrTrp: 0.343 ± 0.024
0.727TyrTyr: 0.727 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1890 proteins (589740 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski