Streptococcus phage P9854

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286QRV1|A0A286QRV1_9CAUD Tail chaperone protein OS=Streptococcus phage P9854 OX=1971446 GN=P9854_14 PE=4 SV=1
MM1 pKa = 6.88EE2 pKa = 4.63QYY4 pKa = 11.41YY5 pKa = 8.65DD6 pKa = 3.34TDD8 pKa = 3.61YY9 pKa = 11.57YY10 pKa = 11.45EE11 pKa = 5.79LMNILNAKK19 pKa = 8.95EE20 pKa = 4.14EE21 pKa = 4.39EE22 pKa = 4.19DD23 pKa = 4.24RR24 pKa = 11.84MVDD27 pKa = 3.51PTSLLL32 pKa = 3.62

Molecular weight: 3.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286QT32|A0A286QT32_9CAUD Uncharacterized protein OS=Streptococcus phage P9854 OX=1971446 GN=P9854_20 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84VRR5 pKa = 11.84YY6 pKa = 9.32LPSDD10 pKa = 3.79FRR12 pKa = 11.84YY13 pKa = 10.09KK14 pKa = 10.89ADD16 pKa = 3.6FGIYY20 pKa = 9.9QSTPNKK26 pKa = 8.23FTGVSVPKK34 pKa = 10.14FVKK37 pKa = 10.38QFTLHH42 pKa = 5.9YY43 pKa = 9.16KK44 pKa = 9.46PHH46 pKa = 6.1TRR48 pKa = 11.84TLNQEE53 pKa = 3.69YY54 pKa = 10.23LAIQNGEE61 pKa = 3.55NDD63 pKa = 3.11TRR65 pKa = 11.84VIVIRR70 pKa = 11.84HH71 pKa = 4.91NSKK74 pKa = 9.81VLEE77 pKa = 4.45GQVVTLNGTQYY88 pKa = 11.15DD89 pKa = 3.86IVRR92 pKa = 11.84ISPDD96 pKa = 3.02EE97 pKa = 3.9NFGFNHH103 pKa = 6.67YY104 pKa = 11.11DD105 pKa = 3.11FLTLKK110 pKa = 10.2KK111 pKa = 9.72RR112 pKa = 11.84KK113 pKa = 9.51KK114 pKa = 10.37VGG116 pKa = 3.08

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11518

32

1602

240.0

27.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.468 ± 0.422

0.651 ± 0.154

6.416 ± 0.24

6.65 ± 0.469

4.358 ± 0.225

6.824 ± 0.408

1.285 ± 0.101

6.52 ± 0.19

8.422 ± 0.624

7.987 ± 0.399

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.18

6.147 ± 0.234

3.152 ± 0.225

4.037 ± 0.216

4.098 ± 0.267

6.486 ± 0.339

6.381 ± 0.431

6.069 ± 0.186

1.476 ± 0.146

4.002 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski