Didymella rabiei (Chickpea ascochyta blight fungus) (Mycosphaerella rabiei)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Ascochyta

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A163LFD8|A0A163LFD8_DIDRA Uncharacterized protein OS=Didymella rabiei OX=5454 GN=ST47_g1231 PE=4 SV=1
MM1 pKa = 7.39SGIVRR6 pKa = 11.84RR7 pKa = 11.84LCTAVIATSAIAAFTVGCGSDD28 pKa = 3.53IEE30 pKa = 4.36PKK32 pKa = 10.53AIAEE36 pKa = 4.34SSSSVADD43 pKa = 3.48STTTSAAPTTSKK55 pKa = 9.11ITGQEE60 pKa = 4.05GSDD63 pKa = 3.77DD64 pKa = 4.21GGDD67 pKa = 3.23VDD69 pKa = 4.49IDD71 pKa = 3.89VSIGDD76 pKa = 4.12CVKK79 pKa = 11.08LGGTTTAAEE88 pKa = 4.54IDD90 pKa = 3.79NADD93 pKa = 4.46CGSKK97 pKa = 10.46DD98 pKa = 3.44SNYY101 pKa = 10.35KK102 pKa = 9.98VVAKK106 pKa = 10.8VPTSDD111 pKa = 4.18LCASDD116 pKa = 3.33VDD118 pKa = 3.91SYY120 pKa = 11.91YY121 pKa = 11.09YY122 pKa = 8.68EE123 pKa = 4.08TLAGDD128 pKa = 3.73EE129 pKa = 4.11QGAVCLDD136 pKa = 3.53VDD138 pKa = 4.07WVVGGCMDD146 pKa = 5.15LGSGMDD152 pKa = 3.35EE153 pKa = 4.21PARR156 pKa = 11.84IEE158 pKa = 4.22CSDD161 pKa = 3.4TSGTNVVEE169 pKa = 4.33VVEE172 pKa = 4.26ILQNSTSIDD181 pKa = 3.6EE182 pKa = 4.9CGSGADD188 pKa = 3.95SGFEE192 pKa = 3.77HH193 pKa = 7.72PEE195 pKa = 3.75RR196 pKa = 11.84KK197 pKa = 8.35FTVCVATLL205 pKa = 3.24

Molecular weight: 20.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A163HDM6|A0A163HDM6_DIDRA 4 iron 4 sulfur cluster binding OS=Didymella rabiei OX=5454 GN=ST47_g3628 PE=3 SV=1
MM1 pKa = 7.97PLTSSRR7 pKa = 11.84AHH9 pKa = 6.26RR10 pKa = 11.84TTATPRR16 pKa = 11.84TTRR19 pKa = 11.84TTRR22 pKa = 11.84SSTAHH27 pKa = 6.92PSLKK31 pKa = 10.46SKK33 pKa = 10.81LLGGGTNRR41 pKa = 11.84RR42 pKa = 11.84TATKK46 pKa = 9.56RR47 pKa = 11.84NPVTGSTTTTTTTTKK62 pKa = 7.41TTRR65 pKa = 11.84TTGGPHH71 pKa = 5.79TAGHH75 pKa = 6.4HH76 pKa = 4.67TTATPVHH83 pKa = 5.5HH84 pKa = 7.27HH85 pKa = 6.4KK86 pKa = 10.61RR87 pKa = 11.84HH88 pKa = 4.81ATMGDD93 pKa = 3.57KK94 pKa = 11.06VSGALTKK101 pKa = 10.88LKK103 pKa = 10.69GSLTGRR109 pKa = 11.84PGVKK113 pKa = 9.91AAGTRR118 pKa = 11.84RR119 pKa = 11.84MHH121 pKa = 5.92GTDD124 pKa = 2.72GRR126 pKa = 11.84NARR129 pKa = 11.84RR130 pKa = 11.84VYY132 pKa = 10.89

Molecular weight: 14.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10558

0

10558

5395534

66

9488

511.0

56.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.255 ± 0.023

1.146 ± 0.009

5.762 ± 0.015

6.069 ± 0.022

3.596 ± 0.014

6.794 ± 0.022

2.507 ± 0.013

4.588 ± 0.016

4.892 ± 0.019

8.689 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.01

3.624 ± 0.014

6.133 ± 0.029

4.136 ± 0.021

6.071 ± 0.022

8.085 ± 0.028

6.218 ± 0.019

6.146 ± 0.018

1.431 ± 0.01

2.729 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski