Serratia phage BF

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eneladusvirus; Serratia virus BF

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6UAB1|A0A1S6UAB1_9CAUD Uncharacterized protein OS=Serratia phage BF OX=1962671 GN=BF_0092 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84VLIMSIYY9 pKa = 10.71NDD11 pKa = 3.35YY12 pKa = 8.93KK13 pKa = 9.84TKK15 pKa = 10.32KK16 pKa = 9.77NEE18 pKa = 3.66YY19 pKa = 8.95FTFLNSNKK27 pKa = 9.79NDD29 pKa = 3.27IFNSAIEE36 pKa = 4.02EE37 pKa = 4.2LKK39 pKa = 11.19LLIPDD44 pKa = 3.98VKK46 pKa = 10.14TIGIIGYY53 pKa = 7.81TPGFNDD59 pKa = 5.37GEE61 pKa = 4.62PCTHH65 pKa = 6.61SSYY68 pKa = 11.75SFFDD72 pKa = 3.92DD73 pKa = 3.72FGEE76 pKa = 4.49LIEE79 pKa = 4.68LSDD82 pKa = 4.08YY83 pKa = 11.05NSEE86 pKa = 4.09VLSCFNIPEE95 pKa = 4.39DD96 pKa = 4.2VLDD99 pKa = 4.25EE100 pKa = 4.81DD101 pKa = 5.12EE102 pKa = 5.0SDD104 pKa = 3.43IYY106 pKa = 11.67DD107 pKa = 4.15WISDD111 pKa = 3.73NTSLKK116 pKa = 9.44LTSADD121 pKa = 3.7RR122 pKa = 11.84QLGNAIMSDD131 pKa = 4.04LEE133 pKa = 4.37ALAEE137 pKa = 4.92DD138 pKa = 3.0IWYY141 pKa = 8.92TDD143 pKa = 3.81FIIMVDD149 pKa = 3.86LSEE152 pKa = 4.16NTPVVKK158 pKa = 10.32HH159 pKa = 6.24IEE161 pKa = 4.26YY162 pKa = 9.91QCGYY166 pKa = 10.45

Molecular weight: 19.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6UAJ7|A0A1S6UAJ7_9CAUD GTP cyclohydrolase OS=Serratia phage BF OX=1962671 GN=BF_0169 PE=4 SV=1
MM1 pKa = 7.18KK2 pKa = 10.38RR3 pKa = 11.84KK4 pKa = 9.09IVKK7 pKa = 9.78RR8 pKa = 11.84NKK10 pKa = 10.33AKK12 pKa = 8.3MTFWINNPFWYY23 pKa = 9.97KK24 pKa = 10.21VVHH27 pKa = 5.58QNRR30 pKa = 11.84EE31 pKa = 3.97HH32 pKa = 7.41FEE34 pKa = 3.65QTRR37 pKa = 11.84NRR39 pKa = 11.84KK40 pKa = 9.7KK41 pKa = 8.91YY42 pKa = 10.47VEE44 pKa = 4.34VFDD47 pKa = 4.46HH48 pKa = 6.0VNAYY52 pKa = 10.42RR53 pKa = 11.84ITEE56 pKa = 3.81RR57 pKa = 11.84SEE59 pKa = 4.71RR60 pKa = 11.84IMVPILITSKK70 pKa = 10.34VKK72 pKa = 10.39RR73 pKa = 11.84SRR75 pKa = 11.84IKK77 pKa = 10.79NRR79 pKa = 11.84LHH81 pKa = 7.38AYY83 pKa = 10.1RR84 pKa = 11.84SFKK87 pKa = 10.79NLEE90 pKa = 4.0EE91 pKa = 4.21MNNEE95 pKa = 4.03CGTAYY100 pKa = 9.7TIDD103 pKa = 4.05EE104 pKa = 4.91IYY106 pKa = 10.95VGWRR110 pKa = 3.04

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

549

0

549

108066

32

3339

196.8

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.503 ± 0.148

1.147 ± 0.055

6.548 ± 0.114

6.999 ± 0.171

4.484 ± 0.088

5.987 ± 0.15

1.927 ± 0.067

7.045 ± 0.09

6.38 ± 0.153

7.873 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.825 ± 0.067

6.256 ± 0.136

3.275 ± 0.072

3.552 ± 0.071

3.92 ± 0.085

6.977 ± 0.117

6.509 ± 0.237

6.767 ± 0.098

1.172 ± 0.04

4.854 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski