Novosphingobium umbonatum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3564 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A437MXG9|A0A437MXG9_9SPHN Phosphatidylserine decarboxylase proenzyme OS=Novosphingobium umbonatum OX=1908524 GN=psd PE=3 SV=1
MM1 pKa = 7.32ATPIFVNEE9 pKa = 3.83LHH11 pKa = 6.55YY12 pKa = 11.49DD13 pKa = 3.49NAGTDD18 pKa = 3.08TGEE21 pKa = 4.68AIEE24 pKa = 4.27IAAPAGTNLAGWSIVLYY41 pKa = 10.9NGGTTGTAAVTYY53 pKa = 7.82GTIALSGTVANQGNGYY69 pKa = 8.0GTLSFAATGIQNGPVDD85 pKa = 4.53GFALVNASGTVVQFLSYY102 pKa = 10.33EE103 pKa = 4.28GVITASNGPASGMTSTDD120 pKa = 2.51IGVSQTGSQASGITLGLTGTGTTYY144 pKa = 11.36EE145 pKa = 4.04NFTWSTLTTGNFGSINNGQTFGSVAVPKK173 pKa = 10.72YY174 pKa = 10.85GEE176 pKa = 4.04LSIANASVTEE186 pKa = 4.43GNSGTQSLSFTVTRR200 pKa = 11.84ANGSDD205 pKa = 3.56GAVSASYY212 pKa = 9.62TVTLNSADD220 pKa = 4.0ASDD223 pKa = 4.23LAAGTTLTGTVSFAAGQTSANIVLGVLGDD252 pKa = 3.82TLVEE256 pKa = 4.21GDD258 pKa = 3.52EE259 pKa = 4.44TLTVTLSAPTGGATLGSVTTATGTITNDD287 pKa = 3.13DD288 pKa = 3.71TALPQAAAIAFINEE302 pKa = 3.39FHH304 pKa = 7.02YY305 pKa = 11.22DD306 pKa = 4.25DD307 pKa = 4.87IGTDD311 pKa = 2.98TGEE314 pKa = 4.54AIEE317 pKa = 4.26IAGTAGLNLAGWTLVLYY334 pKa = 10.36NGNGGAAYY342 pKa = 7.99KK343 pKa = 9.95TIALSGVIADD353 pKa = 3.76QDD355 pKa = 4.02SGFGTLSFAATDD367 pKa = 3.83IQNGSPDD374 pKa = 4.17GIALVDD380 pKa = 3.58ASGTVRR386 pKa = 11.84EE387 pKa = 4.26YY388 pKa = 10.6ISYY391 pKa = 9.48EE392 pKa = 3.88GVMTATSGAANGMVSEE408 pKa = 4.44DD409 pKa = 3.28VGVQEE414 pKa = 4.5NSAPEE419 pKa = 4.01GTSIQRR425 pKa = 11.84VGTGSDD431 pKa = 3.16PAAFTWVTGATSSFGSVNGSQDD453 pKa = 3.39FTPANPNGTLRR464 pKa = 11.84MGNASVVEE472 pKa = 4.24GDD474 pKa = 3.69SGTTAITFTVTRR486 pKa = 11.84SGGVTGAVSADD497 pKa = 3.44YY498 pKa = 11.37NVMFGTATADD508 pKa = 3.76DD509 pKa = 4.48LSGATSGTVNFATGQTSATITLHH532 pKa = 5.51VAADD536 pKa = 3.84TTGEE540 pKa = 3.95PDD542 pKa = 3.2EE543 pKa = 4.55MFSLVLSNAMGGADD557 pKa = 3.31IGRR560 pKa = 11.84SSGTGIIEE568 pKa = 4.33NDD570 pKa = 3.35DD571 pKa = 3.6KK572 pKa = 11.63TRR574 pKa = 11.84VTIMEE579 pKa = 4.11IQGEE583 pKa = 4.61SHH585 pKa = 6.17SSAYY589 pKa = 10.18AGQEE593 pKa = 3.89VTTKK597 pKa = 11.05GIVTAVDD604 pKa = 3.41GAGFYY609 pKa = 10.93LQDD612 pKa = 3.64AAGDD616 pKa = 4.22GNSRR620 pKa = 11.84TSDD623 pKa = 2.92AVFVYY628 pKa = 9.84TGTAPTVKK636 pKa = 9.72IGDD639 pKa = 4.58AIEE642 pKa = 4.07VTATVTEE649 pKa = 4.61YY650 pKa = 11.19VSSTTGSLSVTEE662 pKa = 4.01MTAPVITVEE671 pKa = 4.19SSGNALPAAVLIGPDD686 pKa = 3.6GLHH689 pKa = 6.54IPTANLEE696 pKa = 4.35DD697 pKa = 4.66DD698 pKa = 4.56GFTSFDD704 pKa = 3.97PATDD708 pKa = 4.16GLDD711 pKa = 3.25FWEE714 pKa = 4.61SLEE717 pKa = 3.84GMRR720 pKa = 11.84VEE722 pKa = 4.19IQNPVAIAPTATTYY736 pKa = 11.52GEE738 pKa = 4.42IYY740 pKa = 10.08TVASDD745 pKa = 4.3GAGHH749 pKa = 6.73TSSSGLSAEE758 pKa = 4.07STLAINGSYY767 pKa = 8.93STTGTGTNNSVNGDD781 pKa = 3.79YY782 pKa = 10.96NPEE785 pKa = 4.51KK786 pKa = 10.45IQIDD790 pKa = 3.62ADD792 pKa = 3.85VEE794 pKa = 4.54LGSGTAPLVNPGDD807 pKa = 3.74VLASVVGVVNYY818 pKa = 10.48AGGMYY823 pKa = 10.07EE824 pKa = 4.21VLATQAITIAQDD836 pKa = 3.21TTVAAQTTSLIAGTGQLAVANYY858 pKa = 10.01NVLNLDD864 pKa = 4.09PNDD867 pKa = 4.06ADD869 pKa = 4.01GDD871 pKa = 3.96TDD873 pKa = 3.78VASGRR878 pKa = 11.84FTMIAQDD885 pKa = 2.98IAFALGAPGIVVLEE899 pKa = 4.53EE900 pKa = 3.85IQDD903 pKa = 3.75NDD905 pKa = 3.17GSANTTTVSASLTLQMLVDD924 pKa = 4.95EE925 pKa = 5.65IYY927 pKa = 10.53AASGIQYY934 pKa = 10.8AWIDD938 pKa = 3.37NTYY941 pKa = 8.79ITDD944 pKa = 3.99DD945 pKa = 3.91NNGGEE950 pKa = 4.19PGGNIRR956 pKa = 11.84VAMIYY961 pKa = 10.24RR962 pKa = 11.84VDD964 pKa = 3.61TVDD967 pKa = 3.53LVEE970 pKa = 5.45GSVQTILPTSGSNPFDD986 pKa = 3.98GARR989 pKa = 11.84LPLVASFTFEE999 pKa = 4.28GEE1001 pKa = 3.98EE1002 pKa = 3.79LTVIGNHH1009 pKa = 5.3LTSKK1013 pKa = 10.7GGSTPLQGDD1022 pKa = 3.81VQPSTNAGEE1031 pKa = 4.34AARR1034 pKa = 11.84VAQADD1039 pKa = 3.83VVNDD1043 pKa = 3.74YY1044 pKa = 11.25VDD1046 pKa = 3.95SLLASDD1052 pKa = 4.88PSAHH1056 pKa = 6.57ILVSGDD1062 pKa = 3.28FNDD1065 pKa = 4.4FQFEE1069 pKa = 4.22EE1070 pKa = 4.37PLGVISGEE1078 pKa = 4.0LTLTDD1083 pKa = 3.83GVLTATDD1090 pKa = 3.91GVLTNLNTTIAAQDD1104 pKa = 3.31RR1105 pKa = 11.84YY1106 pKa = 11.36SYY1108 pKa = 10.62IFEE1111 pKa = 4.48GNAQEE1116 pKa = 4.22IDD1118 pKa = 4.05HH1119 pKa = 6.75ILVSDD1124 pKa = 3.99SLLDD1128 pKa = 3.76GAALDD1133 pKa = 3.68IVHH1136 pKa = 6.55VNTLTDD1142 pKa = 3.63RR1143 pKa = 11.84AASDD1147 pKa = 3.79HH1148 pKa = 7.01DD1149 pKa = 4.11PVVALLNLTTSLAVQTGTSGNDD1171 pKa = 3.31TLKK1174 pKa = 10.44GTDD1177 pKa = 3.21GRR1179 pKa = 11.84DD1180 pKa = 3.1RR1181 pKa = 11.84LIGGEE1186 pKa = 4.19GNDD1189 pKa = 3.69VLKK1192 pKa = 11.02GGAGADD1198 pKa = 3.3IMVGGAGDD1206 pKa = 3.48DD1207 pKa = 3.41AYY1209 pKa = 11.47YY1210 pKa = 10.85VDD1212 pKa = 3.92NAADD1216 pKa = 3.48IVTEE1220 pKa = 3.96AAGEE1224 pKa = 4.31GKK1226 pKa = 10.26DD1227 pKa = 3.17RR1228 pKa = 11.84VYY1230 pKa = 11.55ASVSYY1235 pKa = 9.51TLADD1239 pKa = 3.5TLEE1242 pKa = 4.26TLNLTGSDD1250 pKa = 3.38NLTGTGNAQDD1260 pKa = 3.82NQITGNDD1267 pKa = 4.14GDD1269 pKa = 3.91NLLYY1273 pKa = 11.03GLGGKK1278 pKa = 7.84DD1279 pKa = 3.42TLSGGAGADD1288 pKa = 3.32TLYY1291 pKa = 11.06GGEE1294 pKa = 4.73GNDD1297 pKa = 3.77TLKK1300 pKa = 11.43GEE1302 pKa = 4.62AGNDD1306 pKa = 3.73TLVGGAGADD1315 pKa = 4.02SLWGGAGADD1324 pKa = 2.89RR1325 pKa = 11.84FVFDD1329 pKa = 3.94VLEE1332 pKa = 4.32TATSKK1337 pKa = 11.09DD1338 pKa = 3.41AIGDD1342 pKa = 3.81FTSGEE1347 pKa = 4.14DD1348 pKa = 3.48KK1349 pKa = 11.15LVFDD1353 pKa = 5.23LSVFSSLSATGGVIDD1368 pKa = 4.99ASNIAFGGYY1377 pKa = 7.86ATTADD1382 pKa = 3.58QNLLYY1387 pKa = 10.22VASSGTLYY1395 pKa = 10.95YY1396 pKa = 10.32DD1397 pKa = 3.23TDD1399 pKa = 4.08GVGGADD1405 pKa = 2.61RR1406 pKa = 11.84VLIAVLTSKK1415 pKa = 10.42PVLSAADD1422 pKa = 3.83FVAAA1426 pKa = 4.98

Molecular weight: 143.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S2YAN4|A0A3S2YAN4_9SPHN Sporulation protein SsgA OS=Novosphingobium umbonatum OX=1908524 GN=EOE18_05325 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.79NLSAA44 pKa = 4.73

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3564

0

3564

1221789

41

1997

342.8

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.422 ± 0.053

0.839 ± 0.014

5.48 ± 0.03

5.141 ± 0.036

3.481 ± 0.022

8.87 ± 0.043

2.107 ± 0.019

4.693 ± 0.026

3.363 ± 0.027

10.131 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.02

2.795 ± 0.031

5.202 ± 0.03

3.69 ± 0.025

6.555 ± 0.041

5.491 ± 0.035

5.143 ± 0.037

7.099 ± 0.032

1.511 ± 0.019

2.383 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski