Verrucomicrobiae bacterium DG1235

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; unclassified Verrucomicrobiae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5JDM4|B5JDM4_9BACT 4Fe-4S binding domain protein OS=Verrucomicrobiae bacterium DG1235 OX=382464 GN=VDG1235_3466 PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.42PWQDD6 pKa = 3.62YY7 pKa = 10.57SHH9 pKa = 7.66LDD11 pKa = 3.41PSAVPGVTPWTSQLDD26 pKa = 3.55ADD28 pKa = 4.78LAGATPSAPTITNPFPGLTEE48 pKa = 3.51WRR50 pKa = 11.84YY51 pKa = 10.21DD52 pKa = 3.55LVGAGVAQFVVGNYY66 pKa = 9.45SVFDD70 pKa = 3.88SVEE73 pKa = 4.46SIALTITKK81 pKa = 10.06KK82 pKa = 9.78DD83 pKa = 3.67TQPLGVFQWTLFLAGNYY100 pKa = 8.93IYY102 pKa = 10.9NSSAPIGLPEE112 pKa = 4.64LMNVPIVNNSGVTEE126 pKa = 4.09YY127 pKa = 11.51VDD129 pKa = 3.53LAGPGFSLFGYY140 pKa = 8.51VAGVFGEE147 pKa = 4.15VDD149 pKa = 3.19TGSGSDD155 pKa = 3.94LPNLPGIISQIEE167 pKa = 4.09SGAVSFDD174 pKa = 2.95GSYY177 pKa = 9.58VAGYY181 pKa = 9.73FMKK184 pKa = 10.68QSADD188 pKa = 3.07GDD190 pKa = 4.06FIDD193 pKa = 5.66ANFNGQYY200 pKa = 9.86PIEE203 pKa = 5.0ASDD206 pKa = 3.99VPDD209 pKa = 3.86SGSTAVLAALGLVSLLFVRR228 pKa = 11.84RR229 pKa = 11.84SIKK232 pKa = 9.72WIRR235 pKa = 3.26

Molecular weight: 24.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5JDE6|B5JDE6_9BACT ATPase histidine kinase- DNA gyrase B- and HSP90-like domain protein OS=Verrucomicrobiae bacterium DG1235 OX=382464 GN=VDG1235_1967 PE=4 SV=1
MM1 pKa = 7.42SALASDD7 pKa = 4.55IIRR10 pKa = 11.84YY11 pKa = 9.09ARR13 pKa = 11.84GAKK16 pKa = 8.91IQRR19 pKa = 11.84FRR21 pKa = 11.84YY22 pKa = 8.74CRR24 pKa = 11.84RR25 pKa = 11.84PANPNSPHH33 pKa = 6.58AALSAAIAALKK44 pKa = 10.92AEE46 pKa = 4.2LRR48 pKa = 11.84LANSRR53 pKa = 11.84PICQTSAAEE62 pKa = 4.05LLKK65 pKa = 10.74EE66 pKa = 4.07LAALGHH72 pKa = 5.65QPPCQGHH79 pKa = 5.24TEE81 pKa = 4.05QKK83 pKa = 10.32LAAQIVNRR91 pKa = 11.84ALLEE95 pKa = 3.9IRR97 pKa = 4.4

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4883

0

4883

1699424

37

15483

348.0

38.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.026 ± 0.035

0.904 ± 0.017

5.882 ± 0.048

7.062 ± 0.042

4.429 ± 0.021

7.626 ± 0.053

1.904 ± 0.024

5.8 ± 0.032

4.586 ± 0.049

10.081 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.022

3.666 ± 0.03

4.461 ± 0.029

3.358 ± 0.023

5.607 ± 0.043

7.267 ± 0.04

5.265 ± 0.065

6.615 ± 0.039

1.461 ± 0.02

2.947 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski