Psychromicrobium lacuslunae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Psychromicrobium

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0D4BYY9|A0A0D4BYY9_9MICC Uncharacterized protein OS=Psychromicrobium lacuslunae OX=1618207 GN=UM93_07055 PE=4 SV=1
MM1 pKa = 7.68PKK3 pKa = 10.56ALIIVDD9 pKa = 3.88VQNDD13 pKa = 3.63FCEE16 pKa = 4.91GGSLAVSGGEE26 pKa = 3.96EE27 pKa = 4.08VATGISEE34 pKa = 4.84LLDD37 pKa = 3.34STFGAEE43 pKa = 4.06SRR45 pKa = 11.84EE46 pKa = 3.72FDD48 pKa = 4.02YY49 pKa = 11.64VLATQDD55 pKa = 2.96WHH57 pKa = 6.99IDD59 pKa = 3.55PGSHH63 pKa = 7.03FSEE66 pKa = 4.47TPDD69 pKa = 5.8FIDD72 pKa = 3.23SWPVHH77 pKa = 5.93CVAGSKK83 pKa = 10.15GAQLHH88 pKa = 6.53PNLDD92 pKa = 3.59TEE94 pKa = 4.48YY95 pKa = 10.48VDD97 pKa = 4.28AYY99 pKa = 10.22FRR101 pKa = 11.84KK102 pKa = 10.09GKK104 pKa = 9.54YY105 pKa = 7.67QAAYY109 pKa = 10.34SGFEE113 pKa = 4.02GFLAPEE119 pKa = 4.68DD120 pKa = 4.12EE121 pKa = 4.87VPTGPRR127 pKa = 11.84NPEE130 pKa = 3.51QTEE133 pKa = 3.89NPEE136 pKa = 4.03NSEE139 pKa = 4.02SSGDD143 pKa = 3.54VGYY146 pKa = 10.94DD147 pKa = 3.4GDD149 pKa = 5.37DD150 pKa = 4.48DD151 pKa = 5.5GPSLDD156 pKa = 3.46EE157 pKa = 3.92WLQEE161 pKa = 4.07HH162 pKa = 6.29EE163 pKa = 4.38VDD165 pKa = 3.68SVSIVGLATDD175 pKa = 3.58YY176 pKa = 10.89CVRR179 pKa = 11.84ATALDD184 pKa = 3.82AVSAGYY190 pKa = 10.01EE191 pKa = 4.02VTLLSEE197 pKa = 4.56LCAGIDD203 pKa = 3.64PAGSQAALTEE213 pKa = 4.12LTEE216 pKa = 4.59AGVEE220 pKa = 4.36III222 pKa = 5.11

Molecular weight:
23.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0D4BZ54|A0A0D4BZ54_9MICC HTH tetR-type domain-containing protein OS=Psychromicrobium lacuslunae OX=1618207 GN=UM93_08905 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.31VRR4 pKa = 11.84NSLRR8 pKa = 11.84ALKK11 pKa = 9.71KK12 pKa = 10.76LPGAQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 8.58NNPRR34 pKa = 11.84MKK36 pKa = 10.38ARR38 pKa = 11.84QGG40 pKa = 3.24

Molecular weight:
4.66 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2887

0

2887

921680

37

3915

319.3

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.35 ± 0.062

0.577 ± 0.01

5.123 ± 0.033

5.977 ± 0.055

3.343 ± 0.032

8.411 ± 0.042

1.884 ± 0.022

4.807 ± 0.039

3.126 ± 0.041

10.957 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.832 ± 0.019

2.75 ± 0.035

5.03 ± 0.027

4.033 ± 0.03

6.065 ± 0.047

6.783 ± 0.045

5.422 ± 0.049

7.902 ± 0.04

1.464 ± 0.024

2.162 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski