Streptomyces sp. CB01249

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5DY20|A0A1Q5DY20_9ACTN Sugar ABC transporter permease OS=Streptomyces sp. CB01249 OX=1703929 GN=AMK18_19150 PE=3 SV=1
MM1 pKa = 7.31SKK3 pKa = 10.48EE4 pKa = 3.79NPAEE8 pKa = 3.88QHH10 pKa = 5.98HH11 pKa = 6.75GGWQPTPQGGDD22 pKa = 3.21YY23 pKa = 10.94DD24 pKa = 4.31GEE26 pKa = 4.08ATAFVQLPPEE36 pKa = 4.49DD37 pKa = 4.12LADD40 pKa = 3.86IPLAAPGHH48 pKa = 6.64GYY50 pKa = 7.51TPPMILPLTPAADD63 pKa = 4.33RR64 pKa = 11.84DD65 pKa = 3.57PAAAGNWVVQTPEE78 pKa = 3.88QQPGQPAPDD87 pKa = 3.5AVHH90 pKa = 6.67WPDD93 pKa = 3.91PNQQSGYY100 pKa = 9.97GYY102 pKa = 8.45PHH104 pKa = 7.64PEE106 pKa = 4.1DD107 pKa = 3.57QQPTGSYY114 pKa = 9.58QEE116 pKa = 4.62PYY118 pKa = 9.08GTDD121 pKa = 3.4PAATGQWAVDD131 pKa = 3.74TSSWPAPAAPLSDD144 pKa = 4.64DD145 pKa = 3.67SGEE148 pKa = 4.74LYY150 pKa = 10.2TPPPVADD157 pKa = 4.22WYY159 pKa = 10.88ADD161 pKa = 3.98RR162 pKa = 11.84PATLPGGAPAPWAVPDD178 pKa = 3.8AAEE181 pKa = 4.1AAVPEE186 pKa = 4.33AAEE189 pKa = 4.18VTEE192 pKa = 4.6PEE194 pKa = 4.65AVDD197 pKa = 3.9APADD201 pKa = 3.76ADD203 pKa = 3.96EE204 pKa = 5.05PADD207 pKa = 3.9ALAGGPAEE215 pKa = 4.46SAPEE219 pKa = 3.8AAEE222 pKa = 4.1PDD224 pKa = 3.73AEE226 pKa = 4.36QPAPEE231 pKa = 4.76ADD233 pKa = 3.43ALAAADD239 pKa = 4.14PVAAEE244 pKa = 4.61PGAEE248 pKa = 3.96PAEE251 pKa = 4.33PAGEE255 pKa = 4.03AVPDD259 pKa = 3.86AEE261 pKa = 4.41VPEE264 pKa = 5.05AEE266 pKa = 3.96AVQPAEE272 pKa = 4.36VPEE275 pKa = 4.43APAPEE280 pKa = 4.9GPPLPSEE287 pKa = 4.7HH288 pKa = 6.88PAASYY293 pKa = 9.99VLHH296 pKa = 6.36VNGVDD301 pKa = 4.64RR302 pKa = 11.84PVTDD306 pKa = 2.96AWIGEE311 pKa = 4.15SLLYY315 pKa = 10.18VLRR318 pKa = 11.84EE319 pKa = 3.94RR320 pKa = 11.84LGLAGAKK327 pKa = 9.72DD328 pKa = 3.75GCSQGEE334 pKa = 4.12CGACNVQVDD343 pKa = 3.94GRR345 pKa = 11.84LVASCLVPAATTAGSEE361 pKa = 3.99VRR363 pKa = 11.84TVEE366 pKa = 3.9GLAVDD371 pKa = 4.82GEE373 pKa = 4.31PSDD376 pKa = 3.93VQRR379 pKa = 11.84ALADD383 pKa = 3.82CGAVQCGFCIPGMAMTVHH401 pKa = 7.14DD402 pKa = 5.1LLEE405 pKa = 4.95GNHH408 pKa = 6.22RR409 pKa = 11.84PSEE412 pKa = 4.25LEE414 pKa = 3.67TRR416 pKa = 11.84RR417 pKa = 11.84ALCGNLCRR425 pKa = 11.84CSGYY429 pKa = 10.39RR430 pKa = 11.84GVLDD434 pKa = 3.98AVNEE438 pKa = 4.35VIAGRR443 pKa = 11.84EE444 pKa = 3.72AAAEE448 pKa = 3.96AVAEE452 pKa = 4.28EE453 pKa = 4.48TGSGEE458 pKa = 4.07PEE460 pKa = 4.27EE461 pKa = 4.52PRR463 pKa = 11.84IPHH466 pKa = 5.36QAAPGAGSVQAHH478 pKa = 4.76QQDD481 pKa = 3.47GGMAA485 pKa = 3.59

Molecular weight: 49.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5E2J8|A0A1Q5E2J8_9ACTN 3-methyladenine DNA glycosylase OS=Streptomyces sp. CB01249 OX=1703929 GN=AMK18_12655 PE=4 SV=1
MM1 pKa = 8.03ILEE4 pKa = 4.28TVSAVVLGVALSWAALRR21 pKa = 11.84SLPGRR26 pKa = 11.84LPARR30 pKa = 11.84RR31 pKa = 11.84TVFGTGALGGLFGASLMHH49 pKa = 6.54SVLGPHH55 pKa = 6.58HH56 pKa = 6.65AAAVVVGAVLIAAVTVSLLIRR77 pKa = 11.84PRR79 pKa = 11.84SRR81 pKa = 11.84RR82 pKa = 11.84LRR84 pKa = 11.84RR85 pKa = 11.84SAAAA89 pKa = 3.76

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6533

2

6535

2242174

33

7970

343.1

36.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.807 ± 0.049

0.761 ± 0.009

6.09 ± 0.024

5.658 ± 0.028

2.739 ± 0.018

9.596 ± 0.03

2.238 ± 0.015

3.129 ± 0.022

2.219 ± 0.028

10.355 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.728 ± 0.012

1.801 ± 0.019

6.129 ± 0.026

2.658 ± 0.02

7.828 ± 0.034

5.009 ± 0.021

6.226 ± 0.027

8.396 ± 0.032

1.491 ± 0.013

2.14 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski