Ipomoea nil (Japanese morning glory) (Pharbitis nil)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3R2T1|U3R2T1_IPONI Cytochrome b6-f complex subunit 5 OS=Ipomoea nil OX=35883 GN=petG PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 5.22LPGPIHH8 pKa = 7.65DD9 pKa = 4.5FLLVFLGSGLILGSIGVVLFPNPIYY34 pKa = 10.75SAFSLGLVLVCISLFYY50 pKa = 10.63ILSNSYY56 pKa = 9.81FVAAAQLLIYY66 pKa = 10.36VGAINVLILFAVMFLNGSEE85 pKa = 4.15YY86 pKa = 11.32SNDD89 pKa = 3.22FPLWTLGDD97 pKa = 4.69GITSQVCISLFISLISTILDD117 pKa = 3.58TSWYY121 pKa = 9.51GIIWTTRR128 pKa = 11.84SNQIIEE134 pKa = 4.03QDD136 pKa = 4.35LISNSQQIGIHH147 pKa = 6.4LSTDD151 pKa = 4.05FFLPFEE157 pKa = 5.24LISIILLVALIGAIVIARR175 pKa = 11.84QQ176 pKa = 2.99

Molecular weight: 19.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B4ZB18|A0A1B4ZB18_IPONI Ribosomal protein S1 OS=Ipomoea nil OX=35883 GN=rps1 PE=4 SV=1
MM1 pKa = 7.4SLSLLQPYY9 pKa = 10.5FLMSKK14 pKa = 7.59TRR16 pKa = 11.84SYY18 pKa = 11.83AKK20 pKa = 10.06ILIGSRR26 pKa = 11.84LFLTAMAIHH35 pKa = 6.84LSLRR39 pKa = 11.84VAPLDD44 pKa = 3.69LQQGGNSRR52 pKa = 11.84ILYY55 pKa = 7.65VHH57 pKa = 6.4VPAARR62 pKa = 11.84MSILVYY68 pKa = 10.27IVTAINTFLFLLTKK82 pKa = 10.41HH83 pKa = 6.2PLFLRR88 pKa = 11.84SSGTGIEE95 pKa = 4.0MGAFSTLFTLVTGGFRR111 pKa = 11.84GRR113 pKa = 11.84PMWGTFWVWDD123 pKa = 3.63ARR125 pKa = 11.84LTSVFILFLIYY136 pKa = 10.54LGALRR141 pKa = 11.84FQKK144 pKa = 10.7LPVEE148 pKa = 4.25PAPISIRR155 pKa = 11.84AGPIDD160 pKa = 3.49IPIIKK165 pKa = 10.39SSVNWWNTLHH175 pKa = 6.14QPGSISRR182 pKa = 11.84SGTSIHH188 pKa = 6.5VPMPIPILSNFANSPFSTRR207 pKa = 11.84ILFVLEE213 pKa = 3.8TRR215 pKa = 11.84LPIPSFLEE223 pKa = 4.05SPLTEE228 pKa = 4.55EE229 pKa = 3.87IEE231 pKa = 4.1KK232 pKa = 10.51RR233 pKa = 11.84IPKK236 pKa = 9.56PSSLAEE242 pKa = 4.04SLCIHH247 pKa = 6.9GG248 pKa = 4.82

Molecular weight: 27.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

6

109

31905

29

2201

292.7

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.927 ± 0.389

1.241 ± 0.077

3.78 ± 0.172

5.112 ± 0.363

6.034 ± 0.322

6.858 ± 0.383

2.357 ± 0.158

8.215 ± 0.231

5.168 ± 0.507

10.44 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.376 ± 0.119

4.263 ± 0.285

4.454 ± 0.15

3.485 ± 0.158

6.24 ± 0.286

8.118 ± 0.311

5.225 ± 0.143

5.673 ± 0.283

1.617 ± 0.12

3.416 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski