Amino acid dipepetide frequency for Ramlibacter sp. 18x22-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.766AlaAla: 19.766 ± 0.17
1.327AlaCys: 1.327 ± 0.036
6.32AlaAsp: 6.32 ± 0.1
6.473AlaGlu: 6.473 ± 0.088
4.264AlaPhe: 4.264 ± 0.065
12.394AlaGly: 12.394 ± 0.161
2.557AlaHis: 2.557 ± 0.057
5.101AlaIle: 5.101 ± 0.068
4.093AlaLys: 4.093 ± 0.083
14.195AlaLeu: 14.195 ± 0.157
3.707AlaMet: 3.707 ± 0.067
2.961AlaAsn: 2.961 ± 0.099
6.572AlaPro: 6.572 ± 0.107
5.864AlaGln: 5.864 ± 0.075
9.476AlaArg: 9.476 ± 0.127
6.913AlaSer: 6.913 ± 0.1
6.239AlaThr: 6.239 ± 0.124
9.622AlaVal: 9.622 ± 0.094
2.165AlaTrp: 2.165 ± 0.05
2.488AlaTyr: 2.488 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
1.158CysAla: 1.158 ± 0.034
0.123CysCys: 0.123 ± 0.011
0.521CysAsp: 0.521 ± 0.02
0.544CysGlu: 0.544 ± 0.022
0.283CysPhe: 0.283 ± 0.013
1.023CysGly: 1.023 ± 0.035
0.241CysHis: 0.241 ± 0.014
0.395CysIle: 0.395 ± 0.017
0.205CysLys: 0.205 ± 0.013
0.755CysLeu: 0.755 ± 0.025
0.189CysMet: 0.189 ± 0.011
0.2CysAsn: 0.2 ± 0.013
0.449CysPro: 0.449 ± 0.02
0.213CysGln: 0.213 ± 0.012
0.595CysArg: 0.595 ± 0.023
0.481CysSer: 0.481 ± 0.024
0.481CysThr: 0.481 ± 0.019
0.717CysVal: 0.717 ± 0.024
0.139CysTrp: 0.139 ± 0.01
0.183CysTyr: 0.183 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.922AspAla: 6.922 ± 0.094
0.397AspCys: 0.397 ± 0.02
2.537AspAsp: 2.537 ± 0.049
3.171AspGlu: 3.171 ± 0.057
2.019AspPhe: 2.019 ± 0.043
4.685AspGly: 4.685 ± 0.122
1.04AspHis: 1.04 ± 0.028
2.127AspIle: 2.127 ± 0.045
1.536AspLys: 1.536 ± 0.042
5.247AspLeu: 5.247 ± 0.071
1.017AspMet: 1.017 ± 0.028
1.154AspAsn: 1.154 ± 0.026
3.017AspPro: 3.017 ± 0.049
1.543AspGln: 1.543 ± 0.037
3.639AspArg: 3.639 ± 0.053
2.248AspSer: 2.248 ± 0.061
2.497AspThr: 2.497 ± 0.075
4.111AspVal: 4.111 ± 0.06
0.964AspTrp: 0.964 ± 0.033
1.283AspTyr: 1.283 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
6.922GluAla: 6.922 ± 0.101
0.402GluCys: 0.402 ± 0.021
2.354GluAsp: 2.354 ± 0.048
2.797GluGlu: 2.797 ± 0.057
1.768GluPhe: 1.768 ± 0.039
4.151GluGly: 4.151 ± 0.068
1.273GluHis: 1.273 ± 0.037
2.306GluIle: 2.306 ± 0.047
2.068GluLys: 2.068 ± 0.051
5.912GluLeu: 5.912 ± 0.093
1.246GluMet: 1.246 ± 0.031
1.166GluAsn: 1.166 ± 0.031
2.833GluPro: 2.833 ± 0.058
2.562GluGln: 2.562 ± 0.053
4.558GluArg: 4.558 ± 0.072
2.555GluSer: 2.555 ± 0.041
2.35GluThr: 2.35 ± 0.041
4.175GluVal: 4.175 ± 0.072
0.781GluTrp: 0.781 ± 0.026
1.015GluTyr: 1.015 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.364PheAla: 4.364 ± 0.072
0.354PheCys: 0.354 ± 0.015
2.38PheAsp: 2.38 ± 0.037
2.071PheGlu: 2.071 ± 0.043
1.3PhePhe: 1.3 ± 0.043
3.481PheGly: 3.481 ± 0.06
0.754PheHis: 0.754 ± 0.026
1.369PheIle: 1.369 ± 0.036
0.982PheLys: 0.982 ± 0.032
2.979PheLeu: 2.979 ± 0.056
0.711PheMet: 0.711 ± 0.023
1.018PheAsn: 1.018 ± 0.03
1.547PhePro: 1.547 ± 0.042
1.07PheGln: 1.07 ± 0.029
2.091PheArg: 2.091 ± 0.041
1.983PheSer: 1.983 ± 0.051
2.007PheThr: 2.007 ± 0.075
2.645PheVal: 2.645 ± 0.051
0.523PheTrp: 0.523 ± 0.022
0.803PheTyr: 0.803 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.222GlyAla: 10.222 ± 0.161
0.917GlyCys: 0.917 ± 0.027
4.202GlyAsp: 4.202 ± 0.066
4.797GlyGlu: 4.797 ± 0.08
3.279GlyPhe: 3.279 ± 0.063
8.172GlyGly: 8.172 ± 0.17
1.902GlyHis: 1.902 ± 0.037
3.901GlyIle: 3.901 ± 0.059
3.205GlyLys: 3.205 ± 0.064
8.847GlyLeu: 8.847 ± 0.095
2.254GlyMet: 2.254 ± 0.041
2.336GlyAsn: 2.336 ± 0.075
3.42GlyPro: 3.42 ± 0.051
3.522GlyGln: 3.522 ± 0.061
6.17GlyArg: 6.17 ± 0.1
4.843GlySer: 4.843 ± 0.113
5.406GlyThr: 5.406 ± 0.262
6.739GlyVal: 6.739 ± 0.075
1.608GlyTrp: 1.608 ± 0.043
2.282GlyTyr: 2.282 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.763HisAla: 2.763 ± 0.056
0.254HisCys: 0.254 ± 0.014
1.14HisAsp: 1.14 ± 0.033
1.239HisGlu: 1.239 ± 0.033
0.833HisPhe: 0.833 ± 0.028
2.14HisGly: 2.14 ± 0.05
0.602HisHis: 0.602 ± 0.024
0.725HisIle: 0.725 ± 0.021
0.527HisLys: 0.527 ± 0.021
2.176HisLeu: 2.176 ± 0.05
0.444HisMet: 0.444 ± 0.016
0.436HisAsn: 0.436 ± 0.019
1.392HisPro: 1.392 ± 0.034
0.709HisGln: 0.709 ± 0.024
1.525HisArg: 1.525 ± 0.041
0.951HisSer: 0.951 ± 0.027
0.913HisThr: 0.913 ± 0.026
1.687HisVal: 1.687 ± 0.042
0.408HisTrp: 0.408 ± 0.017
0.551HisTyr: 0.551 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.803IleAla: 5.803 ± 0.072
0.376IleCys: 0.376 ± 0.018
2.594IleAsp: 2.594 ± 0.045
2.678IleGlu: 2.678 ± 0.05
1.079IlePhe: 1.079 ± 0.033
3.931IleGly: 3.931 ± 0.061
0.746IleHis: 0.746 ± 0.022
1.132IleIle: 1.132 ± 0.035
1.11IleLys: 1.11 ± 0.028
2.928IleLeu: 2.928 ± 0.057
0.623IleMet: 0.623 ± 0.025
1.143IleAsn: 1.143 ± 0.033
1.878IlePro: 1.878 ± 0.046
1.159IleGln: 1.159 ± 0.029
2.443IleArg: 2.443 ± 0.049
1.978IleSer: 1.978 ± 0.047
2.272IleThr: 2.272 ± 0.077
3.365IleVal: 3.365 ± 0.064
0.377IleTrp: 0.377 ± 0.02
0.812IleTyr: 0.812 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.182LysAla: 4.182 ± 0.077
0.169LysCys: 0.169 ± 0.012
1.684LysAsp: 1.684 ± 0.042
1.528LysGlu: 1.528 ± 0.039
0.961LysPhe: 0.961 ± 0.026
2.388LysGly: 2.388 ± 0.052
0.652LysHis: 0.652 ± 0.022
1.237LysIle: 1.237 ± 0.034
1.372LysLys: 1.372 ± 0.052
3.646LysLeu: 3.646 ± 0.063
0.735LysMet: 0.735 ± 0.026
0.832LysAsn: 0.832 ± 0.028
2.132LysPro: 2.132 ± 0.04
1.138LysGln: 1.138 ± 0.031
2.16LysArg: 2.16 ± 0.047
1.632LysSer: 1.632 ± 0.043
1.702LysThr: 1.702 ± 0.04
2.659LysVal: 2.659 ± 0.058
0.365LysTrp: 0.365 ± 0.016
0.663LysTyr: 0.663 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
15.3LeuAla: 15.3 ± 0.16
0.926LeuCys: 0.926 ± 0.025
5.65LeuAsp: 5.65 ± 0.078
5.217LeuGlu: 5.217 ± 0.085
3.245LeuPhe: 3.245 ± 0.062
8.554LeuGly: 8.554 ± 0.1
2.336LeuHis: 2.336 ± 0.045
3.32LeuIle: 3.32 ± 0.058
3.532LeuLys: 3.532 ± 0.061
11.043LeuLeu: 11.043 ± 0.143
2.335LeuMet: 2.335 ± 0.044
2.545LeuAsn: 2.545 ± 0.054
6.263LeuPro: 6.263 ± 0.083
4.292LeuGln: 4.292 ± 0.068
8.124LeuArg: 8.124 ± 0.115
5.686LeuSer: 5.686 ± 0.076
5.098LeuThr: 5.098 ± 0.119
8.356LeuVal: 8.356 ± 0.104
1.369LeuTrp: 1.369 ± 0.04
1.927LeuTyr: 1.927 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
3.101MetAla: 3.101 ± 0.05
0.169MetCys: 0.169 ± 0.011
1.206MetAsp: 1.206 ± 0.027
1.09MetGlu: 1.09 ± 0.028
0.716MetPhe: 0.716 ± 0.022
1.859MetGly: 1.859 ± 0.042
0.497MetHis: 0.497 ± 0.019
0.779MetIle: 0.779 ± 0.024
1.082MetLys: 1.082 ± 0.028
2.469MetLeu: 2.469 ± 0.052
0.503MetMet: 0.503 ± 0.018
0.787MetAsn: 0.787 ± 0.023
1.517MetPro: 1.517 ± 0.039
0.998MetGln: 0.998 ± 0.025
1.737MetArg: 1.737 ± 0.037
1.409MetSer: 1.409 ± 0.031
1.393MetThr: 1.393 ± 0.034
1.651MetVal: 1.651 ± 0.039
0.207MetTrp: 0.207 ± 0.011
0.39MetTyr: 0.39 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.24AsnAla: 3.24 ± 0.094
0.231AsnCys: 0.231 ± 0.012
1.303AsnAsp: 1.303 ± 0.073
1.221AsnGlu: 1.221 ± 0.033
0.9AsnPhe: 0.9 ± 0.028
2.342AsnGly: 2.342 ± 0.118
0.462AsnHis: 0.462 ± 0.02
1.047AsnIle: 1.047 ± 0.031
0.708AsnLys: 0.708 ± 0.026
2.581AsnLeu: 2.581 ± 0.052
0.505AsnMet: 0.505 ± 0.019
0.72AsnAsn: 0.72 ± 0.033
1.729AsnPro: 1.729 ± 0.04
0.832AsnGln: 0.832 ± 0.03
1.63AsnArg: 1.63 ± 0.034
1.131AsnSer: 1.131 ± 0.044
1.319AsnThr: 1.319 ± 0.06
2.13AsnVal: 2.13 ± 0.076
0.398AsnTrp: 0.398 ± 0.018
0.63AsnTyr: 0.63 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
8.014ProAla: 8.014 ± 0.114
0.369ProCys: 0.369 ± 0.017
3.033ProAsp: 3.033 ± 0.051
3.33ProGlu: 3.33 ± 0.063
1.813ProPhe: 1.813 ± 0.034
5.098ProGly: 5.098 ± 0.069
1.099ProHis: 1.099 ± 0.03
1.699ProIle: 1.699 ± 0.033
1.558ProLys: 1.558 ± 0.039
5.275ProLeu: 5.275 ± 0.075
1.324ProMet: 1.324 ± 0.031
1.262ProAsn: 1.262 ± 0.028
3.058ProPro: 3.058 ± 0.061
2.368ProGln: 2.368 ± 0.043
3.382ProArg: 3.382 ± 0.058
2.89ProSer: 2.89 ± 0.052
2.447ProThr: 2.447 ± 0.064
4.652ProVal: 4.652 ± 0.078
0.844ProTrp: 0.844 ± 0.026
1.076ProTyr: 1.076 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
5.828GlnAla: 5.828 ± 0.077
0.27GlnCys: 0.27 ± 0.016
1.799GlnAsp: 1.799 ± 0.04
1.752GlnGlu: 1.752 ± 0.043
1.159GlnPhe: 1.159 ± 0.029
3.171GlnGly: 3.171 ± 0.057
0.845GlnHis: 0.845 ± 0.027
1.23GlnIle: 1.23 ± 0.03
1.125GlnLys: 1.125 ± 0.033
4.522GlnLeu: 4.522 ± 0.06
0.908GlnMet: 0.908 ± 0.027
0.723GlnAsn: 0.723 ± 0.028
2.536GlnPro: 2.536 ± 0.053
1.856GlnGln: 1.856 ± 0.046
3.413GlnArg: 3.413 ± 0.066
1.932GlnSer: 1.932 ± 0.045
1.542GlnThr: 1.542 ± 0.038
3.423GlnVal: 3.423 ± 0.053
0.67GlnTrp: 0.67 ± 0.024
0.759GlnTyr: 0.759 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
8.344ArgAla: 8.344 ± 0.11
0.621ArgCys: 0.621 ± 0.021
3.624ArgAsp: 3.624 ± 0.058
4.932ArgGlu: 4.932 ± 0.074
2.686ArgPhe: 2.686 ± 0.046
5.244ArgGly: 5.244 ± 0.078
1.836ArgHis: 1.836 ± 0.043
3.307ArgIle: 3.307 ± 0.058
2.294ArgLys: 2.294 ± 0.051
7.925ArgLeu: 7.925 ± 0.115
2.048ArgMet: 2.048 ± 0.041
1.794ArgAsn: 1.794 ± 0.033
3.592ArgPro: 3.592 ± 0.061
2.998ArgGln: 2.998 ± 0.051
5.807ArgArg: 5.807 ± 0.091
3.505ArgSer: 3.505 ± 0.061
3.521ArgThr: 3.521 ± 0.057
5.371ArgVal: 5.371 ± 0.076
1.352ArgTrp: 1.352 ± 0.038
1.748ArgTyr: 1.748 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.322SerAla: 6.322 ± 0.087
0.459SerCys: 0.459 ± 0.018
2.277SerAsp: 2.277 ± 0.053
2.297SerGlu: 2.297 ± 0.043
1.994SerPhe: 1.994 ± 0.048
5.281SerGly: 5.281 ± 0.111
1.124SerHis: 1.124 ± 0.028
2.207SerIle: 2.207 ± 0.067
1.394SerLys: 1.394 ± 0.035
5.62SerLeu: 5.62 ± 0.074
1.282SerMet: 1.282 ± 0.031
1.295SerAsn: 1.295 ± 0.047
2.997SerPro: 2.997 ± 0.056
1.885SerGln: 1.885 ± 0.038
3.817SerArg: 3.817 ± 0.065
3.057SerSer: 3.057 ± 0.07
2.805SerThr: 2.805 ± 0.075
3.933SerVal: 3.933 ± 0.075
0.778SerTrp: 0.778 ± 0.025
1.238SerTyr: 1.238 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
6.332ThrAla: 6.332 ± 0.151
0.41ThrCys: 0.41 ± 0.019
2.352ThrAsp: 2.352 ± 0.072
2.144ThrGlu: 2.144 ± 0.046
1.869ThrPhe: 1.869 ± 0.061
5.201ThrGly: 5.201 ± 0.18
1.027ThrHis: 1.027 ± 0.031
2.032ThrIle: 2.032 ± 0.062
1.268ThrLys: 1.268 ± 0.031
5.924ThrLeu: 5.924 ± 0.125
1.059ThrMet: 1.059 ± 0.03
1.276ThrAsn: 1.276 ± 0.064
3.403ThrPro: 3.403 ± 0.063
1.834ThrGln: 1.834 ± 0.05
3.217ThrArg: 3.217 ± 0.052
2.74ThrSer: 2.74 ± 0.084
2.963ThrThr: 2.963 ± 0.164
4.562ThrVal: 4.562 ± 0.223
0.72ThrTrp: 0.72 ± 0.026
1.254ThrTyr: 1.254 ± 0.136
0.0ThrXaa: 0.0 ± 0.0
Val
9.91ValAla: 9.91 ± 0.096
0.696ValCys: 0.696 ± 0.026
4.193ValAsp: 4.193 ± 0.057
4.13ValGlu: 4.13 ± 0.064
2.808ValPhe: 2.808 ± 0.048
5.761ValGly: 5.761 ± 0.072
1.655ValHis: 1.655 ± 0.041
3.204ValIle: 3.204 ± 0.064
2.612ValLys: 2.612 ± 0.043
8.751ValLeu: 8.751 ± 0.103
1.869ValMet: 1.869 ± 0.043
2.366ValAsn: 2.366 ± 0.118
4.437ValPro: 4.437 ± 0.068
3.098ValGln: 3.098 ± 0.048
5.744ValArg: 5.744 ± 0.081
4.124ValSer: 4.124 ± 0.107
4.506ValThr: 4.506 ± 0.159
6.786ValVal: 6.786 ± 0.079
0.999ValTrp: 0.999 ± 0.029
1.577ValTyr: 1.577 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.514TrpAla: 1.514 ± 0.041
0.191TrpCys: 0.191 ± 0.012
0.693TrpAsp: 0.693 ± 0.022
0.565TrpGlu: 0.565 ± 0.021
0.605TrpPhe: 0.605 ± 0.022
1.069TrpGly: 1.069 ± 0.031
0.354TrpHis: 0.354 ± 0.017
0.616TrpIle: 0.616 ± 0.022
0.533TrpLys: 0.533 ± 0.022
2.096TrpLeu: 2.096 ± 0.048
0.414TrpMet: 0.414 ± 0.018
0.457TrpAsn: 0.457 ± 0.019
0.777TrpPro: 0.777 ± 0.025
0.759TrpGln: 0.759 ± 0.023
1.383TrpArg: 1.383 ± 0.034
0.841TrpSer: 0.841 ± 0.027
0.798TrpThr: 0.798 ± 0.026
1.033TrpVal: 1.033 ± 0.033
0.316TrpTrp: 0.316 ± 0.017
0.333TrpTyr: 0.333 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.494TyrAla: 2.494 ± 0.048
0.234TyrCys: 0.234 ± 0.015
1.293TyrAsp: 1.293 ± 0.074
1.216TyrGlu: 1.216 ± 0.032
0.85TyrPhe: 0.85 ± 0.025
2.076TyrGly: 2.076 ± 0.045
0.412TyrHis: 0.412 ± 0.017
0.69TyrIle: 0.69 ± 0.021
0.631TyrLys: 0.631 ± 0.022
2.228TyrLeu: 2.228 ± 0.052
0.387TyrMet: 0.387 ± 0.019
0.59TyrAsn: 0.59 ± 0.029
1.043TyrPro: 1.043 ± 0.027
0.765TyrGln: 0.765 ± 0.028
1.641TyrArg: 1.641 ± 0.036
1.165TyrSer: 1.165 ± 0.037
1.327TyrThr: 1.327 ± 0.109
1.608TyrVal: 1.608 ± 0.035
0.363TyrTrp: 0.363 ± 0.017
0.555TyrTyr: 0.555 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4139 proteins (1368773 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski