Amino acid dipepetide frequency for Escherichia phage T7 (Bacteriophage T7)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.497AlaAla: 9.497 ± 1.484
0.804AlaCys: 0.804 ± 0.275
5.552AlaAsp: 5.552 ± 0.628
5.552AlaGlu: 5.552 ± 0.525
2.411AlaPhe: 2.411 ± 0.424
6.867AlaGly: 6.867 ± 0.844
1.388AlaHis: 1.388 ± 0.291
5.698AlaIle: 5.698 ± 0.692
6.721AlaLys: 6.721 ± 0.662
9.204AlaLeu: 9.204 ± 0.954
3.36AlaMet: 3.36 ± 0.444
4.091AlaAsn: 4.091 ± 0.468
1.972AlaPro: 1.972 ± 0.283
3.141AlaGln: 3.141 ± 0.655
5.698AlaArg: 5.698 ± 0.565
5.041AlaSer: 5.041 ± 0.626
3.433AlaThr: 3.433 ± 0.567
4.821AlaVal: 4.821 ± 0.674
1.534AlaTrp: 1.534 ± 0.351
2.557AlaTyr: 2.557 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
1.096CysAla: 1.096 ± 0.354
0.146CysCys: 0.146 ± 0.105
1.315CysAsp: 1.315 ± 0.417
0.365CysGlu: 0.365 ± 0.2
0.511CysPhe: 0.511 ± 0.217
0.877CysGly: 0.877 ± 0.285
0.731CysHis: 0.731 ± 0.294
0.657CysIle: 0.657 ± 0.218
0.731CysLys: 0.731 ± 0.299
0.804CysLeu: 0.804 ± 0.26
0.073CysMet: 0.073 ± 0.07
0.365CysAsn: 0.365 ± 0.16
0.438CysPro: 0.438 ± 0.151
0.438CysGln: 0.438 ± 0.152
0.511CysArg: 0.511 ± 0.214
1.096CysSer: 1.096 ± 0.295
0.584CysThr: 0.584 ± 0.214
0.877CysVal: 0.877 ± 0.242
0.219CysTrp: 0.219 ± 0.13
0.584CysTyr: 0.584 ± 0.227
0.0CysXaa: 0.0 ± 0.0
Asp
4.675AspAla: 4.675 ± 0.605
0.877AspCys: 0.877 ± 0.28
4.164AspAsp: 4.164 ± 0.742
4.456AspGlu: 4.456 ± 0.488
2.922AspPhe: 2.922 ± 0.285
5.844AspGly: 5.844 ± 0.609
0.731AspHis: 0.731 ± 0.201
3.36AspIle: 3.36 ± 0.463
4.164AspLys: 4.164 ± 0.507
4.164AspLeu: 4.164 ± 0.622
2.63AspMet: 2.63 ± 0.418
2.849AspAsn: 2.849 ± 0.427
2.849AspPro: 2.849 ± 0.563
2.118AspGln: 2.118 ± 0.366
2.703AspArg: 2.703 ± 0.425
3.799AspSer: 3.799 ± 0.55
3.433AspThr: 3.433 ± 0.507
4.383AspVal: 4.383 ± 0.559
1.096AspTrp: 1.096 ± 0.361
1.388AspTyr: 1.388 ± 0.294
0.0AspXaa: 0.0 ± 0.0
Glu
6.94GluAla: 6.94 ± 0.756
0.292GluCys: 0.292 ± 0.137
4.091GluAsp: 4.091 ± 0.601
6.502GluGlu: 6.502 ± 0.952
1.68GluPhe: 1.68 ± 0.342
5.114GluGly: 5.114 ± 0.608
1.461GluHis: 1.461 ± 0.337
3.287GluIle: 3.287 ± 0.441
3.506GluLys: 3.506 ± 0.591
6.209GluLeu: 6.209 ± 0.659
2.557GluMet: 2.557 ± 0.416
3.214GluAsn: 3.214 ± 0.406
1.534GluPro: 1.534 ± 0.34
3.433GluGln: 3.433 ± 0.736
4.091GluArg: 4.091 ± 0.578
4.967GluSer: 4.967 ± 0.764
3.945GluThr: 3.945 ± 0.39
4.529GluVal: 4.529 ± 0.647
1.169GluTrp: 1.169 ± 0.365
3.214GluTyr: 3.214 ± 0.489
0.0GluXaa: 0.0 ± 0.0
Phe
1.899PheAla: 1.899 ± 0.357
0.511PheCys: 0.511 ± 0.181
3.141PheAsp: 3.141 ± 0.504
2.484PheGlu: 2.484 ± 0.43
0.95PhePhe: 0.95 ± 0.276
3.36PheGly: 3.36 ± 0.41
0.657PheHis: 0.657 ± 0.258
1.242PheIle: 1.242 ± 0.372
2.849PheLys: 2.849 ± 0.399
3.068PheLeu: 3.068 ± 0.456
1.315PheMet: 1.315 ± 0.245
1.388PheAsn: 1.388 ± 0.387
1.388PhePro: 1.388 ± 0.356
1.169PheGln: 1.169 ± 0.275
2.045PheArg: 2.045 ± 0.431
1.972PheSer: 1.972 ± 0.474
2.338PheThr: 2.338 ± 0.44
2.045PheVal: 2.045 ± 0.381
0.219PheTrp: 0.219 ± 0.121
1.169PheTyr: 1.169 ± 0.241
0.0PheXaa: 0.0 ± 0.0
Gly
6.355GlyAla: 6.355 ± 0.847
1.315GlyCys: 1.315 ± 0.329
4.091GlyAsp: 4.091 ± 0.557
4.894GlyGlu: 4.894 ± 0.619
3.287GlyPhe: 3.287 ± 0.546
5.114GlyGly: 5.114 ± 0.771
1.534GlyHis: 1.534 ± 0.303
4.675GlyIle: 4.675 ± 0.599
5.917GlyLys: 5.917 ± 0.833
6.648GlyLeu: 6.648 ± 0.89
1.826GlyMet: 1.826 ± 0.361
3.068GlyAsn: 3.068 ± 0.479
0.95GlyPro: 0.95 ± 0.274
3.141GlyGln: 3.141 ± 0.523
4.602GlyArg: 4.602 ± 0.458
4.164GlySer: 4.164 ± 0.572
3.945GlyThr: 3.945 ± 0.485
4.091GlyVal: 4.091 ± 0.441
1.388GlyTrp: 1.388 ± 0.446
3.141GlyTyr: 3.141 ± 0.549
0.0GlyXaa: 0.0 ± 0.0
His
1.096HisAla: 1.096 ± 0.352
0.292HisCys: 0.292 ± 0.129
1.242HisAsp: 1.242 ± 0.277
1.826HisGlu: 1.826 ± 0.372
0.584HisPhe: 0.584 ± 0.176
1.388HisGly: 1.388 ± 0.303
0.511HisHis: 0.511 ± 0.153
1.899HisIle: 1.899 ± 0.441
1.315HisLys: 1.315 ± 0.316
2.63HisLeu: 2.63 ± 0.597
0.584HisMet: 0.584 ± 0.204
0.731HisAsn: 0.731 ± 0.214
0.292HisPro: 0.292 ± 0.139
0.219HisGln: 0.219 ± 0.136
0.804HisArg: 0.804 ± 0.218
1.096HisSer: 1.096 ± 0.273
0.877HisThr: 0.877 ± 0.254
1.242HisVal: 1.242 ± 0.314
0.292HisTrp: 0.292 ± 0.129
1.023HisTyr: 1.023 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
3.726IleAla: 3.726 ± 0.438
0.877IleCys: 0.877 ± 0.237
4.091IleAsp: 4.091 ± 0.604
3.653IleGlu: 3.653 ± 0.682
0.804IlePhe: 0.804 ± 0.233
3.58IleGly: 3.58 ± 0.438
1.315IleHis: 1.315 ± 0.392
3.36IleIle: 3.36 ± 0.582
4.237IleLys: 4.237 ± 0.517
4.529IleLeu: 4.529 ± 0.533
1.461IleMet: 1.461 ± 0.308
2.63IleAsn: 2.63 ± 0.549
2.484IlePro: 2.484 ± 0.363
1.461IleGln: 1.461 ± 0.346
3.433IleArg: 3.433 ± 0.524
2.63IleSer: 2.63 ± 0.382
2.849IleThr: 2.849 ± 0.362
3.214IleVal: 3.214 ± 0.479
0.511IleTrp: 0.511 ± 0.192
1.899IleTyr: 1.899 ± 0.314
0.0IleXaa: 0.0 ± 0.0
Lys
7.378LysAla: 7.378 ± 0.813
0.657LysCys: 0.657 ± 0.232
4.018LysAsp: 4.018 ± 0.479
5.041LysGlu: 5.041 ± 0.687
2.411LysPhe: 2.411 ± 0.376
5.333LysGly: 5.333 ± 0.782
1.68LysHis: 1.68 ± 0.359
2.265LysIle: 2.265 ± 0.45
4.31LysLys: 4.31 ± 0.758
4.894LysLeu: 4.894 ± 0.658
2.265LysMet: 2.265 ± 0.378
2.484LysAsn: 2.484 ± 0.344
2.557LysPro: 2.557 ± 0.603
2.849LysGln: 2.849 ± 0.527
4.31LysArg: 4.31 ± 0.604
4.018LysSer: 4.018 ± 0.518
3.068LysThr: 3.068 ± 0.428
4.748LysVal: 4.748 ± 0.639
0.804LysTrp: 0.804 ± 0.218
2.411LysTyr: 2.411 ± 0.341
0.0LysXaa: 0.0 ± 0.0
Leu
9.204LeuAla: 9.204 ± 1.191
1.023LeuCys: 1.023 ± 0.359
4.018LeuAsp: 4.018 ± 0.518
6.355LeuGlu: 6.355 ± 0.588
2.557LeuPhe: 2.557 ± 0.445
4.821LeuGly: 4.821 ± 0.714
1.607LeuHis: 1.607 ± 0.37
3.945LeuIle: 3.945 ± 0.56
5.114LeuLys: 5.114 ± 0.501
5.406LeuLeu: 5.406 ± 0.719
2.557LeuMet: 2.557 ± 0.372
3.726LeuAsn: 3.726 ± 0.543
2.922LeuPro: 2.922 ± 0.49
3.653LeuGln: 3.653 ± 0.54
6.282LeuArg: 6.282 ± 0.609
5.041LeuSer: 5.041 ± 0.67
5.771LeuThr: 5.771 ± 0.837
4.164LeuVal: 4.164 ± 0.51
1.242LeuTrp: 1.242 ± 0.349
2.265LeuTyr: 2.265 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
2.922MetAla: 2.922 ± 0.484
0.365MetCys: 0.365 ± 0.161
1.972MetAsp: 1.972 ± 0.412
2.045MetGlu: 2.045 ± 0.383
1.242MetPhe: 1.242 ± 0.408
2.265MetGly: 2.265 ± 0.39
0.657MetHis: 0.657 ± 0.218
1.388MetIle: 1.388 ± 0.276
1.096MetLys: 1.096 ± 0.254
3.506MetLeu: 3.506 ± 0.486
0.804MetMet: 0.804 ± 0.254
1.388MetAsn: 1.388 ± 0.355
1.461MetPro: 1.461 ± 0.289
0.95MetGln: 0.95 ± 0.301
1.607MetArg: 1.607 ± 0.246
1.753MetSer: 1.753 ± 0.405
2.265MetThr: 2.265 ± 0.387
2.484MetVal: 2.484 ± 0.376
0.146MetTrp: 0.146 ± 0.12
0.877MetTyr: 0.877 ± 0.265
0.0MetXaa: 0.0 ± 0.0
Asn
3.36AsnAla: 3.36 ± 0.434
0.584AsnCys: 0.584 ± 0.18
2.338AsnAsp: 2.338 ± 0.425
2.63AsnGlu: 2.63 ± 0.418
1.826AsnPhe: 1.826 ± 0.39
5.041AsnGly: 5.041 ± 0.676
0.804AsnHis: 0.804 ± 0.267
2.776AsnIle: 2.776 ± 0.507
2.849AsnLys: 2.849 ± 0.431
3.068AsnLeu: 3.068 ± 0.445
1.023AsnMet: 1.023 ± 0.256
1.826AsnAsn: 1.826 ± 0.459
2.776AsnPro: 2.776 ± 0.453
1.607AsnGln: 1.607 ± 0.266
1.972AsnArg: 1.972 ± 0.626
2.849AsnSer: 2.849 ± 0.599
2.484AsnThr: 2.484 ± 0.639
3.141AsnVal: 3.141 ± 0.499
0.511AsnTrp: 0.511 ± 0.18
1.753AsnTyr: 1.753 ± 0.387
0.0AsnXaa: 0.0 ± 0.0
Pro
2.922ProAla: 2.922 ± 0.438
0.584ProCys: 0.584 ± 0.217
2.703ProAsp: 2.703 ± 0.425
3.872ProGlu: 3.872 ± 0.751
1.388ProPhe: 1.388 ± 0.311
0.584ProGly: 0.584 ± 0.209
0.95ProHis: 0.95 ± 0.278
1.461ProIle: 1.461 ± 0.289
2.411ProLys: 2.411 ± 0.476
2.192ProLeu: 2.192 ± 0.412
1.169ProMet: 1.169 ± 0.326
2.484ProAsn: 2.484 ± 0.543
0.657ProPro: 0.657 ± 0.249
0.95ProGln: 0.95 ± 0.31
1.826ProArg: 1.826 ± 0.459
2.118ProSer: 2.118 ± 0.409
1.68ProThr: 1.68 ± 0.35
1.826ProVal: 1.826 ± 0.298
0.731ProTrp: 0.731 ± 0.174
1.242ProTyr: 1.242 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
3.726GlnAla: 3.726 ± 0.672
0.292GlnCys: 0.292 ± 0.145
1.534GlnAsp: 1.534 ± 0.33
2.484GlnGlu: 2.484 ± 0.518
2.192GlnPhe: 2.192 ± 0.297
2.192GlnGly: 2.192 ± 0.352
0.292GlnHis: 0.292 ± 0.197
1.607GlnIle: 1.607 ± 0.327
2.338GlnLys: 2.338 ± 0.361
3.799GlnLeu: 3.799 ± 0.545
1.534GlnMet: 1.534 ± 0.326
1.315GlnAsn: 1.315 ± 0.282
1.242GlnPro: 1.242 ± 0.405
1.972GlnGln: 1.972 ± 0.27
1.899GlnArg: 1.899 ± 0.452
2.192GlnSer: 2.192 ± 0.52
1.607GlnThr: 1.607 ± 0.384
2.63GlnVal: 2.63 ± 0.486
1.023GlnTrp: 1.023 ± 0.261
0.95GlnTyr: 0.95 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
4.821ArgAla: 4.821 ± 0.561
0.877ArgCys: 0.877 ± 0.25
3.945ArgAsp: 3.945 ± 0.653
4.967ArgGlu: 4.967 ± 0.674
2.265ArgPhe: 2.265 ± 0.416
3.287ArgGly: 3.287 ± 0.459
1.315ArgHis: 1.315 ± 0.313
2.995ArgIle: 2.995 ± 0.436
4.675ArgLys: 4.675 ± 0.685
4.675ArgLeu: 4.675 ± 0.476
1.534ArgMet: 1.534 ± 0.4
2.484ArgAsn: 2.484 ± 0.577
1.899ArgPro: 1.899 ± 0.254
1.899ArgGln: 1.899 ± 0.507
3.068ArgArg: 3.068 ± 0.497
4.237ArgSer: 4.237 ± 0.592
2.63ArgThr: 2.63 ± 0.366
2.922ArgVal: 2.922 ± 0.464
0.877ArgTrp: 0.877 ± 0.244
1.607ArgTyr: 1.607 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
5.406SerAla: 5.406 ± 0.663
0.731SerCys: 0.731 ± 0.277
5.041SerAsp: 5.041 ± 0.65
3.287SerGlu: 3.287 ± 0.404
2.63SerPhe: 2.63 ± 0.474
5.844SerGly: 5.844 ± 0.922
1.68SerHis: 1.68 ± 0.39
2.922SerIle: 2.922 ± 0.536
3.653SerLys: 3.653 ± 0.489
3.945SerLeu: 3.945 ± 0.512
1.534SerMet: 1.534 ± 0.385
3.068SerAsn: 3.068 ± 0.483
1.68SerPro: 1.68 ± 0.366
2.045SerGln: 2.045 ± 0.304
2.776SerArg: 2.776 ± 0.547
3.36SerSer: 3.36 ± 0.538
3.506SerThr: 3.506 ± 0.52
4.456SerVal: 4.456 ± 0.613
1.023SerTrp: 1.023 ± 0.25
2.045SerTyr: 2.045 ± 0.397
0.0SerXaa: 0.0 ± 0.0
Thr
4.675ThrAla: 4.675 ± 0.656
0.804ThrCys: 0.804 ± 0.302
3.506ThrAsp: 3.506 ± 0.442
3.214ThrGlu: 3.214 ± 0.537
1.972ThrPhe: 1.972 ± 0.502
5.479ThrGly: 5.479 ± 0.618
0.95ThrHis: 0.95 ± 0.235
3.287ThrIle: 3.287 ± 0.517
4.821ThrLys: 4.821 ± 0.511
4.529ThrLeu: 4.529 ± 0.548
1.096ThrMet: 1.096 ± 0.293
1.899ThrAsn: 1.899 ± 0.424
2.703ThrPro: 2.703 ± 0.435
2.118ThrGln: 2.118 ± 0.419
2.484ThrArg: 2.484 ± 0.401
3.58ThrSer: 3.58 ± 0.658
3.287ThrThr: 3.287 ± 0.601
3.945ThrVal: 3.945 ± 0.519
0.511ThrTrp: 0.511 ± 0.187
1.315ThrTyr: 1.315 ± 0.227
0.0ThrXaa: 0.0 ± 0.0
Val
5.552ValAla: 5.552 ± 0.482
0.657ValCys: 0.657 ± 0.278
2.849ValAsp: 2.849 ± 0.425
4.456ValGlu: 4.456 ± 0.747
2.338ValPhe: 2.338 ± 0.601
4.164ValGly: 4.164 ± 0.515
0.95ValHis: 0.95 ± 0.252
3.287ValIle: 3.287 ± 0.52
3.58ValLys: 3.58 ± 0.599
4.529ValLeu: 4.529 ± 0.511
2.338ValMet: 2.338 ± 0.41
2.922ValAsn: 2.922 ± 0.583
2.849ValPro: 2.849 ± 0.481
2.118ValGln: 2.118 ± 0.416
4.091ValArg: 4.091 ± 0.539
3.653ValSer: 3.653 ± 0.598
5.26ValThr: 5.26 ± 0.475
4.967ValVal: 4.967 ± 0.782
1.315ValTrp: 1.315 ± 0.273
2.118ValTyr: 2.118 ± 0.453
0.0ValXaa: 0.0 ± 0.0
Trp
0.804TrpAla: 0.804 ± 0.258
0.292TrpCys: 0.292 ± 0.161
0.511TrpAsp: 0.511 ± 0.209
1.315TrpGlu: 1.315 ± 0.318
0.438TrpPhe: 0.438 ± 0.193
0.804TrpGly: 0.804 ± 0.288
0.365TrpHis: 0.365 ± 0.192
0.877TrpIle: 0.877 ± 0.269
1.315TrpLys: 1.315 ± 0.344
1.388TrpLeu: 1.388 ± 0.345
0.292TrpMet: 0.292 ± 0.143
1.461TrpAsn: 1.461 ± 0.377
0.146TrpPro: 0.146 ± 0.104
0.438TrpGln: 0.438 ± 0.188
0.95TrpArg: 0.95 ± 0.274
1.096TrpSer: 1.096 ± 0.305
1.315TrpThr: 1.315 ± 0.285
1.096TrpVal: 1.096 ± 0.386
0.438TrpTrp: 0.438 ± 0.153
0.219TrpTyr: 0.219 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.36TyrAla: 3.36 ± 0.463
0.438TyrCys: 0.438 ± 0.167
2.484TyrAsp: 2.484 ± 0.451
2.338TyrGlu: 2.338 ± 0.454
0.804TyrPhe: 0.804 ± 0.287
2.192TyrGly: 2.192 ± 0.414
0.365TyrHis: 0.365 ± 0.171
1.753TyrIle: 1.753 ± 0.398
1.899TyrLys: 1.899 ± 0.379
2.484TyrLeu: 2.484 ± 0.466
1.169TyrMet: 1.169 ± 0.264
1.826TyrAsn: 1.826 ± 0.305
0.95TyrPro: 0.95 ± 0.272
1.096TyrGln: 1.096 ± 0.365
1.826TyrArg: 1.826 ± 0.374
1.972TyrSer: 1.972 ± 0.376
1.899TyrThr: 1.899 ± 0.375
2.411TyrVal: 2.411 ± 0.471
0.511TyrTrp: 0.511 ± 0.209
0.584TyrTyr: 0.584 ± 0.259
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13690 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski