Vibrio phage VP585

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vhmlvirus; Vibrio virus VP585

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4HTX1|D4HTX1_9CAUD Gp32 protein OS=Vibrio phage VP585 OX=631719 PE=4 SV=1
MM1 pKa = 8.34DD2 pKa = 4.53YY3 pKa = 11.55LDD5 pKa = 4.38LQMLAGAALGLTEE18 pKa = 4.81EE19 pKa = 4.21QTEE22 pKa = 4.73DD23 pKa = 4.62IINDD27 pKa = 3.85DD28 pKa = 4.41EE29 pKa = 6.33DD30 pKa = 4.51FDD32 pKa = 4.71SPLIEE37 pKa = 4.32KK38 pKa = 10.56FGVDD42 pKa = 3.77LEE44 pKa = 4.48QFGEE48 pKa = 4.03IAEE51 pKa = 4.34ALLPFTPTLYY61 pKa = 10.74SEE63 pKa = 4.29LTKK66 pKa = 10.25TVYY69 pKa = 10.17HH70 pKa = 6.23AFVRR74 pKa = 11.84QTGQGSCMAIVKK86 pKa = 9.72QKK88 pKa = 10.69AAEE91 pKa = 4.01QPEE94 pKa = 4.37KK95 pKa = 11.22EE96 pKa = 4.31EE97 pKa = 4.06AA98 pKa = 3.36

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4HTV9|D4HTV9_9CAUD Gp20 protein OS=Vibrio phage VP585 OX=631719 PE=4 SV=1
MM1 pKa = 7.23MMYY4 pKa = 10.06GYY6 pKa = 10.04QAIKK10 pKa = 10.15QFPKK14 pKa = 10.37HH15 pKa = 5.36EE16 pKa = 4.07RR17 pKa = 11.84HH18 pKa = 5.81VLGAEE23 pKa = 3.81IRR25 pKa = 11.84LSMLQLQRR33 pKa = 11.84LIITAFKK40 pKa = 10.24RR41 pKa = 11.84YY42 pKa = 9.03HH43 pKa = 6.55KK44 pKa = 10.04KK45 pKa = 7.24TTLTDD50 pKa = 3.81LDD52 pKa = 3.82IEE54 pKa = 4.19LAILKK59 pKa = 10.21RR60 pKa = 11.84RR61 pKa = 11.84VRR63 pKa = 11.84LAKK66 pKa = 9.82DD67 pKa = 3.24LRR69 pKa = 11.84YY70 pKa = 9.69IDD72 pKa = 3.34IKK74 pKa = 10.75RR75 pKa = 11.84YY76 pKa = 8.91EE77 pKa = 3.88IWVGQLVEE85 pKa = 4.34LGKK88 pKa = 10.28MIGGWIRR95 pKa = 11.84SVNAKK100 pKa = 8.75QQGTALL106 pKa = 3.8

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13153

53

1117

226.8

25.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.884 ± 0.565

1.285 ± 0.17

5.877 ± 0.33

7.071 ± 0.274

3.414 ± 0.187

7.192 ± 0.228

2.159 ± 0.182

4.866 ± 0.212

5.748 ± 0.276

9.063 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.174

3.893 ± 0.223

3.908 ± 0.249

4.79 ± 0.339

5.786 ± 0.248

5.626 ± 0.237

5.421 ± 0.191

7.002 ± 0.299

1.597 ± 0.146

2.897 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski