Amino acid dipepetide frequency for Gordonia phage WilliamBoone

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.61AlaAla: 9.61 ± 0.828
0.652AlaCys: 0.652 ± 0.19
6.864AlaAsp: 6.864 ± 0.394
6.761AlaGlu: 6.761 ± 0.554
2.952AlaPhe: 2.952 ± 0.442
7.31AlaGly: 7.31 ± 0.458
1.819AlaHis: 1.819 ± 0.305
4.53AlaIle: 4.53 ± 0.439
4.05AlaLys: 4.05 ± 0.446
8.649AlaLeu: 8.649 ± 0.542
2.746AlaMet: 2.746 ± 0.355
3.157AlaAsn: 3.157 ± 0.393
4.256AlaPro: 4.256 ± 0.4
4.393AlaGln: 4.393 ± 0.713
6.59AlaArg: 6.59 ± 0.637
4.771AlaSer: 4.771 ± 0.44
5.251AlaThr: 5.251 ± 0.53
6.349AlaVal: 6.349 ± 0.526
1.613AlaTrp: 1.613 ± 0.219
2.368AlaTyr: 2.368 ± 0.248
0.0AlaXaa: 0.0 ± 0.0
Cys
0.824CysAla: 0.824 ± 0.212
0.309CysCys: 0.309 ± 0.106
0.995CysAsp: 0.995 ± 0.226
0.721CysGlu: 0.721 ± 0.178
0.24CysPhe: 0.24 ± 0.091
1.27CysGly: 1.27 ± 0.28
0.446CysHis: 0.446 ± 0.125
0.446CysIle: 0.446 ± 0.136
0.515CysLys: 0.515 ± 0.111
0.549CysLeu: 0.549 ± 0.167
0.069CysMet: 0.069 ± 0.049
0.652CysAsn: 0.652 ± 0.174
0.515CysPro: 0.515 ± 0.13
0.343CysGln: 0.343 ± 0.104
0.686CysArg: 0.686 ± 0.194
0.549CysSer: 0.549 ± 0.181
0.583CysThr: 0.583 ± 0.176
0.789CysVal: 0.789 ± 0.205
0.24CysTrp: 0.24 ± 0.083
0.172CysTyr: 0.172 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
6.349AspAla: 6.349 ± 0.575
0.755AspCys: 0.755 ± 0.196
5.148AspAsp: 5.148 ± 0.659
5.182AspGlu: 5.182 ± 0.524
2.094AspPhe: 2.094 ± 0.282
6.109AspGly: 6.109 ± 0.55
2.094AspHis: 2.094 ± 0.242
3.466AspIle: 3.466 ± 0.361
2.368AspLys: 2.368 ± 0.31
5.869AspLeu: 5.869 ± 0.443
1.682AspMet: 1.682 ± 0.264
1.991AspAsn: 1.991 ± 0.272
4.084AspPro: 4.084 ± 0.422
1.922AspGln: 1.922 ± 0.278
4.771AspArg: 4.771 ± 0.441
2.814AspSer: 2.814 ± 0.3
3.466AspThr: 3.466 ± 0.383
5.697AspVal: 5.697 ± 0.394
1.579AspTrp: 1.579 ± 0.258
2.299AspTyr: 2.299 ± 0.3
0.0AspXaa: 0.0 ± 0.0
Glu
7.139GluAla: 7.139 ± 0.662
0.789GluCys: 0.789 ± 0.197
4.256GluAsp: 4.256 ± 0.394
4.427GluGlu: 4.427 ± 0.404
2.505GluPhe: 2.505 ± 0.301
4.736GluGly: 4.736 ± 0.425
1.373GluHis: 1.373 ± 0.261
4.221GluIle: 4.221 ± 0.441
3.157GluLys: 3.157 ± 0.355
5.937GluLeu: 5.937 ± 0.423
2.059GluMet: 2.059 ± 0.231
2.197GluAsn: 2.197 ± 0.248
3.192GluPro: 3.192 ± 0.712
2.746GluGln: 2.746 ± 0.29
4.324GluArg: 4.324 ± 0.409
3.604GluSer: 3.604 ± 0.394
3.26GluThr: 3.26 ± 0.335
5.457GluVal: 5.457 ± 0.639
2.265GluTrp: 2.265 ± 0.267
2.608GluTyr: 2.608 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
3.055PheAla: 3.055 ± 0.286
0.48PheCys: 0.48 ± 0.136
2.814PheAsp: 2.814 ± 0.285
2.128PheGlu: 2.128 ± 0.321
0.755PhePhe: 0.755 ± 0.192
2.265PheGly: 2.265 ± 0.269
0.686PheHis: 0.686 ± 0.121
1.682PheIle: 1.682 ± 0.256
1.098PheLys: 1.098 ± 0.2
2.162PheLeu: 2.162 ± 0.312
1.03PheMet: 1.03 ± 0.22
1.647PheAsn: 1.647 ± 0.265
1.339PhePro: 1.339 ± 0.203
0.755PheGln: 0.755 ± 0.176
1.613PheArg: 1.613 ± 0.211
1.853PheSer: 1.853 ± 0.229
1.922PheThr: 1.922 ± 0.29
2.059PheVal: 2.059 ± 0.287
0.721PheTrp: 0.721 ± 0.182
0.789PheTyr: 0.789 ± 0.13
0.0PheXaa: 0.0 ± 0.0
Gly
6.006GlyAla: 6.006 ± 0.579
0.858GlyCys: 0.858 ± 0.196
5.217GlyAsp: 5.217 ± 0.534
5.114GlyGlu: 5.114 ± 0.444
2.299GlyPhe: 2.299 ± 0.309
6.178GlyGly: 6.178 ± 0.8
2.059GlyHis: 2.059 ± 0.338
3.501GlyIle: 3.501 ± 0.367
3.741GlyLys: 3.741 ± 0.344
5.8GlyLeu: 5.8 ± 0.495
2.025GlyMet: 2.025 ± 0.25
2.952GlyAsn: 2.952 ± 0.388
3.981GlyPro: 3.981 ± 0.437
2.849GlyGln: 2.849 ± 0.289
4.839GlyArg: 4.839 ± 0.365
4.839GlySer: 4.839 ± 0.46
4.633GlyThr: 4.633 ± 0.452
6.246GlyVal: 6.246 ± 0.413
2.574GlyTrp: 2.574 ± 0.31
2.574GlyTyr: 2.574 ± 0.32
0.0GlyXaa: 0.0 ± 0.0
His
1.613HisAla: 1.613 ± 0.226
0.412HisCys: 0.412 ± 0.124
0.961HisAsp: 0.961 ± 0.206
1.75HisGlu: 1.75 ± 0.228
1.098HisPhe: 1.098 ± 0.208
2.025HisGly: 2.025 ± 0.361
0.412HisHis: 0.412 ± 0.128
1.167HisIle: 1.167 ± 0.236
0.789HisLys: 0.789 ± 0.152
1.922HisLeu: 1.922 ± 0.269
0.446HisMet: 0.446 ± 0.126
0.721HisAsn: 0.721 ± 0.175
1.304HisPro: 1.304 ± 0.262
0.858HisGln: 0.858 ± 0.197
2.162HisArg: 2.162 ± 0.362
1.27HisSer: 1.27 ± 0.206
1.339HisThr: 1.339 ± 0.216
1.75HisVal: 1.75 ± 0.289
0.618HisTrp: 0.618 ± 0.158
0.927HisTyr: 0.927 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.148IleAla: 5.148 ± 0.379
0.446IleCys: 0.446 ± 0.115
4.084IleAsp: 4.084 ± 0.46
4.084IleGlu: 4.084 ± 0.338
0.686IlePhe: 0.686 ± 0.115
3.089IleGly: 3.089 ± 0.291
1.304IleHis: 1.304 ± 0.227
2.128IleIle: 2.128 ± 0.349
2.128IleLys: 2.128 ± 0.3
3.123IleLeu: 3.123 ± 0.385
0.789IleMet: 0.789 ± 0.19
1.991IleAsn: 1.991 ± 0.277
2.574IlePro: 2.574 ± 0.303
1.716IleGln: 1.716 ± 0.245
3.672IleArg: 3.672 ± 0.345
3.157IleSer: 3.157 ± 0.317
2.746IleThr: 2.746 ± 0.3
3.329IleVal: 3.329 ± 0.389
0.961IleTrp: 0.961 ± 0.191
1.373IleTyr: 1.373 ± 0.238
0.0IleXaa: 0.0 ± 0.0
Lys
3.535LysAla: 3.535 ± 0.357
0.48LysCys: 0.48 ± 0.165
2.402LysAsp: 2.402 ± 0.324
2.162LysGlu: 2.162 ± 0.284
1.476LysPhe: 1.476 ± 0.274
3.226LysGly: 3.226 ± 0.364
0.858LysHis: 0.858 ± 0.171
1.544LysIle: 1.544 ± 0.221
2.094LysLys: 2.094 ± 0.286
3.638LysLeu: 3.638 ± 0.374
1.167LysMet: 1.167 ± 0.179
1.373LysAsn: 1.373 ± 0.21
2.54LysPro: 2.54 ± 0.254
1.544LysGln: 1.544 ± 0.249
3.295LysArg: 3.295 ± 0.363
2.643LysSer: 2.643 ± 0.281
2.059LysThr: 2.059 ± 0.278
3.535LysVal: 3.535 ± 0.368
0.755LysTrp: 0.755 ± 0.176
1.064LysTyr: 1.064 ± 0.19
0.0LysXaa: 0.0 ± 0.0
Leu
7.379LeuAla: 7.379 ± 0.691
1.03LeuCys: 1.03 ± 0.233
5.629LeuAsp: 5.629 ± 0.369
6.212LeuGlu: 6.212 ± 0.488
2.231LeuPhe: 2.231 ± 0.27
6.143LeuGly: 6.143 ± 0.554
1.75LeuHis: 1.75 ± 0.303
3.981LeuIle: 3.981 ± 0.361
2.814LeuLys: 2.814 ± 0.336
5.182LeuLeu: 5.182 ± 0.489
1.51LeuMet: 1.51 ± 0.235
3.535LeuAsn: 3.535 ± 0.356
3.604LeuPro: 3.604 ± 0.417
2.78LeuGln: 2.78 ± 0.342
5.354LeuArg: 5.354 ± 0.436
4.736LeuSer: 4.736 ± 0.389
4.736LeuThr: 4.736 ± 0.434
4.53LeuVal: 4.53 ± 0.459
1.441LeuTrp: 1.441 ± 0.21
2.197LeuTyr: 2.197 ± 0.23
0.0LeuXaa: 0.0 ± 0.0
Met
2.917MetAla: 2.917 ± 0.295
0.137MetCys: 0.137 ± 0.066
1.133MetAsp: 1.133 ± 0.212
1.304MetGlu: 1.304 ± 0.21
0.995MetPhe: 0.995 ± 0.166
1.613MetGly: 1.613 ± 0.237
0.652MetHis: 0.652 ± 0.164
1.304MetIle: 1.304 ± 0.208
0.824MetLys: 0.824 ± 0.165
1.201MetLeu: 1.201 ± 0.194
0.378MetMet: 0.378 ± 0.108
1.373MetAsn: 1.373 ± 0.189
1.339MetPro: 1.339 ± 0.213
1.098MetGln: 1.098 ± 0.202
1.819MetArg: 1.819 ± 0.224
2.437MetSer: 2.437 ± 0.257
2.094MetThr: 2.094 ± 0.287
1.441MetVal: 1.441 ± 0.222
0.343MetTrp: 0.343 ± 0.106
0.412MetTyr: 0.412 ± 0.119
0.0MetXaa: 0.0 ± 0.0
Asn
3.604AsnAla: 3.604 ± 0.377
0.686AsnCys: 0.686 ± 0.168
2.299AsnAsp: 2.299 ± 0.277
2.025AsnGlu: 2.025 ± 0.249
1.03AsnPhe: 1.03 ± 0.189
3.398AsnGly: 3.398 ± 0.312
0.995AsnHis: 0.995 ± 0.204
2.025AsnIle: 2.025 ± 0.279
1.476AsnLys: 1.476 ± 0.346
3.638AsnLeu: 3.638 ± 0.341
0.515AsnMet: 0.515 ± 0.12
1.51AsnAsn: 1.51 ± 0.321
2.299AsnPro: 2.299 ± 0.271
1.51AsnGln: 1.51 ± 0.285
2.299AsnArg: 2.299 ± 0.351
1.75AsnSer: 1.75 ± 0.242
1.991AsnThr: 1.991 ± 0.41
2.094AsnVal: 2.094 ± 0.247
0.48AsnTrp: 0.48 ± 0.117
1.098AsnTyr: 1.098 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
4.771ProAla: 4.771 ± 0.458
0.515ProCys: 0.515 ± 0.155
3.81ProAsp: 3.81 ± 0.407
4.256ProGlu: 4.256 ± 0.651
1.956ProPhe: 1.956 ± 0.27
4.221ProGly: 4.221 ± 0.424
0.995ProHis: 0.995 ± 0.204
1.956ProIle: 1.956 ± 0.263
2.368ProLys: 2.368 ± 0.372
2.952ProLeu: 2.952 ± 0.29
1.064ProMet: 1.064 ± 0.201
1.51ProAsn: 1.51 ± 0.225
2.094ProPro: 2.094 ± 0.301
1.441ProGln: 1.441 ± 0.225
3.157ProArg: 3.157 ± 0.415
3.02ProSer: 3.02 ± 0.486
3.02ProThr: 3.02 ± 0.425
2.917ProVal: 2.917 ± 0.336
0.927ProTrp: 0.927 ± 0.177
1.544ProTyr: 1.544 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
3.947GlnAla: 3.947 ± 0.54
0.378GlnCys: 0.378 ± 0.109
2.128GlnAsp: 2.128 ± 0.23
1.922GlnGlu: 1.922 ± 0.306
0.892GlnPhe: 0.892 ± 0.156
2.711GlnGly: 2.711 ± 0.316
0.652GlnHis: 0.652 ± 0.155
1.819GlnIle: 1.819 ± 0.305
1.579GlnLys: 1.579 ± 0.337
3.741GlnLeu: 3.741 ± 0.479
1.407GlnMet: 1.407 ± 0.214
1.544GlnAsn: 1.544 ± 0.297
1.579GlnPro: 1.579 ± 0.227
1.613GlnGln: 1.613 ± 0.396
2.437GlnArg: 2.437 ± 0.51
1.819GlnSer: 1.819 ± 0.316
1.579GlnThr: 1.579 ± 0.266
2.402GlnVal: 2.402 ± 0.266
0.789GlnTrp: 0.789 ± 0.138
0.961GlnTyr: 0.961 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
6.075ArgAla: 6.075 ± 0.504
0.824ArgCys: 0.824 ± 0.194
4.153ArgAsp: 4.153 ± 0.397
6.109ArgGlu: 6.109 ± 0.5
2.231ArgPhe: 2.231 ± 0.26
4.908ArgGly: 4.908 ± 0.39
2.334ArgHis: 2.334 ± 0.405
3.295ArgIle: 3.295 ± 0.314
3.398ArgLys: 3.398 ± 0.352
5.045ArgLeu: 5.045 ± 0.411
1.476ArgMet: 1.476 ± 0.224
2.54ArgAsn: 2.54 ± 0.265
2.814ArgPro: 2.814 ± 0.315
2.197ArgGln: 2.197 ± 0.268
5.732ArgArg: 5.732 ± 0.524
3.741ArgSer: 3.741 ± 0.361
3.604ArgThr: 3.604 ± 0.319
5.217ArgVal: 5.217 ± 0.44
1.785ArgTrp: 1.785 ± 0.27
2.368ArgTyr: 2.368 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
5.697SerAla: 5.697 ± 0.452
0.446SerCys: 0.446 ± 0.115
4.668SerAsp: 4.668 ± 0.416
3.535SerGlu: 3.535 ± 0.331
1.544SerPhe: 1.544 ± 0.235
4.976SerGly: 4.976 ± 0.571
0.961SerHis: 0.961 ± 0.213
2.814SerIle: 2.814 ± 0.276
2.162SerLys: 2.162 ± 0.292
4.29SerLeu: 4.29 ± 0.324
1.682SerMet: 1.682 ± 0.258
1.956SerAsn: 1.956 ± 0.256
2.471SerPro: 2.471 ± 0.277
1.75SerGln: 1.75 ± 0.242
3.81SerArg: 3.81 ± 0.465
3.707SerSer: 3.707 ± 0.418
3.295SerThr: 3.295 ± 0.327
4.324SerVal: 4.324 ± 0.418
1.75SerTrp: 1.75 ± 0.257
2.128SerTyr: 2.128 ± 0.271
0.0SerXaa: 0.0 ± 0.0
Thr
6.006ThrAla: 6.006 ± 0.543
0.515ThrCys: 0.515 ± 0.136
4.118ThrAsp: 4.118 ± 0.381
4.29ThrGlu: 4.29 ± 0.371
1.956ThrPhe: 1.956 ± 0.232
4.565ThrGly: 4.565 ± 0.457
1.133ThrHis: 1.133 ± 0.202
2.986ThrIle: 2.986 ± 0.327
2.643ThrLys: 2.643 ± 0.332
4.118ThrLeu: 4.118 ± 0.435
1.476ThrMet: 1.476 ± 0.271
1.853ThrAsn: 1.853 ± 0.266
3.466ThrPro: 3.466 ± 0.356
1.682ThrGln: 1.682 ± 0.322
3.878ThrArg: 3.878 ± 0.336
3.741ThrSer: 3.741 ± 0.448
3.157ThrThr: 3.157 ± 0.338
3.295ThrVal: 3.295 ± 0.327
1.098ThrTrp: 1.098 ± 0.196
1.888ThrTyr: 1.888 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
6.727ValAla: 6.727 ± 0.544
0.721ValCys: 0.721 ± 0.185
5.491ValAsp: 5.491 ± 0.405
4.805ValGlu: 4.805 ± 0.539
2.334ValPhe: 2.334 ± 0.301
5.594ValGly: 5.594 ± 0.372
1.373ValHis: 1.373 ± 0.232
3.432ValIle: 3.432 ± 0.372
2.574ValLys: 2.574 ± 0.281
4.976ValLeu: 4.976 ± 0.425
1.991ValMet: 1.991 ± 0.241
2.197ValAsn: 2.197 ± 0.309
2.986ValPro: 2.986 ± 0.381
2.402ValGln: 2.402 ± 0.322
5.32ValArg: 5.32 ± 0.401
4.221ValSer: 4.221 ± 0.469
5.182ValThr: 5.182 ± 0.544
5.114ValVal: 5.114 ± 0.458
1.304ValTrp: 1.304 ± 0.214
1.819ValTyr: 1.819 ± 0.287
0.0ValXaa: 0.0 ± 0.0
Trp
1.51TrpAla: 1.51 ± 0.207
0.24TrpCys: 0.24 ± 0.116
1.716TrpAsp: 1.716 ± 0.207
1.613TrpGlu: 1.613 ± 0.216
0.755TrpPhe: 0.755 ± 0.188
1.579TrpGly: 1.579 ± 0.256
0.927TrpHis: 0.927 ± 0.186
1.27TrpIle: 1.27 ± 0.199
0.549TrpLys: 0.549 ± 0.152
1.579TrpLeu: 1.579 ± 0.254
0.686TrpMet: 0.686 ± 0.139
0.892TrpAsn: 0.892 ± 0.196
0.858TrpPro: 0.858 ± 0.178
1.098TrpGln: 1.098 ± 0.18
1.579TrpArg: 1.579 ± 0.205
1.339TrpSer: 1.339 ± 0.244
1.888TrpThr: 1.888 ± 0.293
1.27TrpVal: 1.27 ± 0.209
0.618TrpTrp: 0.618 ± 0.17
0.755TrpTyr: 0.755 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.78TyrAla: 2.78 ± 0.276
0.24TyrCys: 0.24 ± 0.1
1.853TyrAsp: 1.853 ± 0.297
1.853TyrGlu: 1.853 ± 0.302
0.824TyrPhe: 0.824 ± 0.203
2.368TyrGly: 2.368 ± 0.276
0.721TyrHis: 0.721 ± 0.193
0.961TyrIle: 0.961 ± 0.2
1.064TyrLys: 1.064 ± 0.192
2.402TyrLeu: 2.402 ± 0.327
0.583TyrMet: 0.583 ± 0.14
1.133TyrAsn: 1.133 ± 0.227
1.236TyrPro: 1.236 ± 0.266
1.201TyrGln: 1.201 ± 0.186
2.505TyrArg: 2.505 ± 0.357
1.956TyrSer: 1.956 ± 0.268
2.059TyrThr: 2.059 ± 0.265
2.746TyrVal: 2.746 ± 0.341
0.858TyrTrp: 0.858 ± 0.208
0.789TyrTyr: 0.789 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 185 proteins (29138 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski