Proteus phage Saba

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NF04|A0A5B9NF04_9CAUD Uncharacterized protein OS=Proteus phage Saba OX=2596672 GN=CPT_Saba_020 PE=4 SV=1
MM1 pKa = 7.14YY2 pKa = 10.98NVMLSIAGTLAVLMVMFYY20 pKa = 10.94ALYY23 pKa = 9.04WVVEE27 pKa = 4.15AFDD30 pKa = 3.68MGIGLRR36 pKa = 11.84DD37 pKa = 3.51FFGRR41 pKa = 11.84TAAICFVLLVFDD53 pKa = 5.76IILSVAFLFIVKK65 pKa = 9.51IWEE68 pKa = 4.25SLVV71 pKa = 3.31

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NBN8|A0A5B9NBN8_9CAUD Uncharacterized protein OS=Proteus phage Saba OX=2596672 GN=CPT_Saba_037 PE=4 SV=1
MM1 pKa = 7.46SSEE4 pKa = 4.3RR5 pKa = 11.84EE6 pKa = 3.83KK7 pKa = 10.92QAANLLKK14 pKa = 10.53LKK16 pKa = 10.28PGDD19 pKa = 3.94SFFVACLPDD28 pKa = 3.91KK29 pKa = 9.67LTHH32 pKa = 6.13IRR34 pKa = 11.84RR35 pKa = 11.84LGYY38 pKa = 10.42KK39 pKa = 10.33LGIKK43 pKa = 10.41LSFRR47 pKa = 11.84FVAVDD52 pKa = 4.33PIYY55 pKa = 10.97GLAGTRR61 pKa = 11.84VTRR64 pKa = 11.84KK65 pKa = 9.33RR66 pKa = 3.36

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

18900

62

1436

248.7

27.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.635 ± 0.565

1.032 ± 0.138

6.529 ± 0.222

6.735 ± 0.198

4.053 ± 0.183

7.18 ± 0.253

1.566 ± 0.117

5.995 ± 0.138

6.561 ± 0.285

7.434 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.137

4.196 ± 0.171

4.392 ± 0.31

2.984 ± 0.239

5.582 ± 0.207

6.407 ± 0.274

5.624 ± 0.193

7.635 ± 0.323

1.429 ± 0.134

3.339 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski