Streptococcus phage phiARI0131-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141E0P8|A0A141E0P8_9CAUD IstB_IS21 domain-containing protein OS=Streptococcus phage phiARI0131-2 OX=1701814 GN=phiARI0131-2_28 PE=4 SV=1
MM1 pKa = 7.74VGVTYY6 pKa = 9.81QEE8 pKa = 3.08IHH10 pKa = 6.73LFVEE14 pKa = 5.07FLKK17 pKa = 10.63EE18 pKa = 3.59QYY20 pKa = 10.55GQGRR24 pKa = 11.84PDD26 pKa = 3.75YY27 pKa = 10.97IEE29 pKa = 4.84ALNDD33 pKa = 3.49LDD35 pKa = 4.31GLVEE39 pKa = 3.81VSYY42 pKa = 11.25RR43 pKa = 11.84EE44 pKa = 4.08AIEE47 pKa = 5.01RR48 pKa = 11.84FLEE51 pKa = 4.64DD52 pKa = 3.39EE53 pKa = 4.32LL54 pKa = 5.78

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141E0P3|A0A141E0P3_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0131-2 OX=1701814 GN=phiARI0131-2_23 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.08RR4 pKa = 11.84IKK6 pKa = 10.54KK7 pKa = 10.04KK8 pKa = 8.7IAKK11 pKa = 9.53RR12 pKa = 11.84QIQEE16 pKa = 4.14KK17 pKa = 10.06QEE19 pKa = 3.92EE20 pKa = 5.12LYY22 pKa = 10.8KK23 pKa = 10.38QLQKK27 pKa = 11.02LSPEE31 pKa = 4.19EE32 pKa = 3.89IEE34 pKa = 6.03AITKK38 pKa = 8.18MINQLVSNIRR48 pKa = 11.84KK49 pKa = 9.29ALSQIFDD56 pKa = 3.52NLFTFFKK63 pKa = 10.11NLEE66 pKa = 4.01VEE68 pKa = 3.81IEE70 pKa = 4.1KK71 pKa = 10.33IEE73 pKa = 4.03RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84TQNVRR81 pKa = 11.84QRR83 pKa = 11.84TFQISKK89 pKa = 10.03HH90 pKa = 4.36STHH93 pKa = 6.18NRR95 pKa = 11.84FKK97 pKa = 10.79KK98 pKa = 10.49ARR100 pKa = 11.84IRR102 pKa = 11.84NQKK105 pKa = 8.67PRR107 pKa = 11.84CSKK110 pKa = 9.46WAYY113 pKa = 9.82CRR115 pKa = 11.84NKK117 pKa = 8.32QTYY120 pKa = 9.62RR121 pKa = 11.84NYY123 pKa = 10.52RR124 pKa = 11.84NQNRR128 pKa = 11.84GG129 pKa = 2.93

Molecular weight: 15.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

9358

46

2123

252.9

28.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.074 ± 0.644

0.502 ± 0.106

6.07 ± 0.439

7.737 ± 0.416

4.467 ± 0.336

6.038 ± 0.315

1.411 ± 0.19

6.839 ± 0.295

8.271 ± 0.475

8.121 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.299

5.995 ± 0.336

2.607 ± 0.263

4.499 ± 0.406

4.467 ± 0.335

6.187 ± 0.613

6.348 ± 0.464

6.262 ± 0.348

1.304 ± 0.162

3.43 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski