Byssochlamys spectabilis (Paecilomyces variotii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta;

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A443HWV5|A0A443HWV5_BYSSP Dipeptidyl-peptidase IV OS=Byssochlamys spectabilis OX=264951 GN=C8Q69DRAFT_464790 PE=3 SV=1
GG1 pKa = 6.89QGYY4 pKa = 7.82NQPRR8 pKa = 11.84LSQICQEE15 pKa = 4.13TSLDD19 pKa = 3.61IINIGFVNVFPDD31 pKa = 3.76VGQGGWPGSNFGNQCNGQTYY51 pKa = 9.59TNADD55 pKa = 3.51GEE57 pKa = 4.56ATQLLSGCQQIADD70 pKa = 5.4DD71 pKa = 5.56IPVCQQAGKK80 pKa = 10.42KK81 pKa = 9.43VFISLGGAYY90 pKa = 9.13PADD93 pKa = 3.67GQIIDD98 pKa = 4.47NDD100 pKa = 3.42DD101 pKa = 3.53SARR104 pKa = 11.84DD105 pKa = 3.49FADD108 pKa = 3.97FLWGAFGPEE117 pKa = 3.22TDD119 pKa = 3.61AWVAQNGPRR128 pKa = 11.84PFGDD132 pKa = 3.11AVVDD136 pKa = 4.34GFDD139 pKa = 5.15FDD141 pKa = 4.57IEE143 pKa = 4.81HH144 pKa = 6.88NGPTGYY150 pKa = 10.77AALARR155 pKa = 11.84RR156 pKa = 11.84LRR158 pKa = 11.84DD159 pKa = 3.46NFSEE163 pKa = 4.79FPSKK167 pKa = 10.61QFYY170 pKa = 10.82LSAAPQCLSPDD181 pKa = 3.54AQLSNAIQFASFDD194 pKa = 4.54FIWVQFYY201 pKa = 8.58NTDD204 pKa = 3.59PCSARR209 pKa = 11.84AWVDD213 pKa = 2.96GDD215 pKa = 3.73VTSDD219 pKa = 3.32FTFDD223 pKa = 2.65AWIAAIKK230 pKa = 10.66LGGNPSAKK238 pKa = 10.28LFIGLPADD246 pKa = 3.53PTQAYY251 pKa = 8.85YY252 pKa = 10.93VSPDD256 pKa = 3.24EE257 pKa = 4.67VEE259 pKa = 4.41TLVEE263 pKa = 4.55EE264 pKa = 4.33YY265 pKa = 9.62MGRR268 pKa = 11.84YY269 pKa = 8.44PEE271 pKa = 4.15NFGGIMIWEE280 pKa = 4.19ATASDD285 pKa = 4.05EE286 pKa = 4.12NQIDD290 pKa = 3.79GRR292 pKa = 11.84SYY294 pKa = 9.32AANMKK299 pKa = 10.28SVLLL303 pKa = 4.11

Molecular weight: 32.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A443HL20|A0A443HL20_BYSSP Tyrosine--tRNA ligase OS=Byssochlamys spectabilis OX=264951 GN=C8Q69DRAFT_479466 PE=3 SV=1
SS1 pKa = 6.85HH2 pKa = 7.5KK3 pKa = 10.71SFRR6 pKa = 11.84TKK8 pKa = 10.45QKK10 pKa = 9.84LAKK13 pKa = 9.55AQKK16 pKa = 8.59QNRR19 pKa = 11.84PIPQWIRR26 pKa = 11.84LRR28 pKa = 11.84TGNTIRR34 pKa = 11.84YY35 pKa = 5.79NAKK38 pKa = 8.89RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 4.14WRR43 pKa = 11.84KK44 pKa = 7.41TRR46 pKa = 11.84LGII49 pKa = 4.46

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9264

0

9264

4547040

49

7905

490.8

54.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.322 ± 0.019

1.182 ± 0.01

5.693 ± 0.017

6.402 ± 0.024

3.714 ± 0.017

6.75 ± 0.02

2.333 ± 0.01

5.037 ± 0.017

4.883 ± 0.023

8.879 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.009

3.645 ± 0.012

6.09 ± 0.028

3.974 ± 0.018

6.365 ± 0.022

8.514 ± 0.034

5.824 ± 0.016

6.064 ± 0.017

1.414 ± 0.009

2.842 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski