Amino acid dipepetide frequency for Proteus phage PM135

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.415AlaAla: 3.415 ± 0.552
0.884AlaCys: 0.884 ± 0.198
3.375AlaAsp: 3.375 ± 0.401
4.62AlaGlu: 4.62 ± 0.532
3.174AlaPhe: 3.174 ± 0.313
4.74AlaGly: 4.74 ± 0.527
1.446AlaHis: 1.446 ± 0.229
5.022AlaIle: 5.022 ± 0.672
6.789AlaLys: 6.789 ± 0.895
7.151AlaLeu: 7.151 ± 0.643
1.808AlaMet: 1.808 ± 0.287
4.419AlaAsn: 4.419 ± 0.547
1.969AlaPro: 1.969 ± 0.318
3.013AlaGln: 3.013 ± 0.422
3.053AlaArg: 3.053 ± 0.283
4.781AlaSer: 4.781 ± 0.478
4.459AlaThr: 4.459 ± 0.568
4.058AlaVal: 4.058 ± 0.428
0.723AlaTrp: 0.723 ± 0.159
2.451AlaTyr: 2.451 ± 0.343
0.0AlaXaa: 0.0 ± 0.0
Cys
0.482CysAla: 0.482 ± 0.151
0.08CysCys: 0.08 ± 0.053
0.643CysAsp: 0.643 ± 0.143
0.482CysGlu: 0.482 ± 0.141
0.362CysPhe: 0.362 ± 0.107
0.924CysGly: 0.924 ± 0.23
0.321CysHis: 0.321 ± 0.099
0.723CysIle: 0.723 ± 0.178
1.045CysLys: 1.045 ± 0.232
0.562CysLeu: 0.562 ± 0.148
0.121CysMet: 0.121 ± 0.069
0.603CysAsn: 0.603 ± 0.149
0.562CysPro: 0.562 ± 0.149
0.201CysGln: 0.201 ± 0.104
0.482CysArg: 0.482 ± 0.12
1.045CysSer: 1.045 ± 0.236
0.603CysThr: 0.603 ± 0.157
0.362CysVal: 0.362 ± 0.118
0.08CysTrp: 0.08 ± 0.052
0.442CysTyr: 0.442 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
5.142AspAla: 5.142 ± 0.553
0.522AspCys: 0.522 ± 0.148
2.37AspAsp: 2.37 ± 0.356
3.254AspGlu: 3.254 ± 0.354
3.294AspPhe: 3.294 ± 0.381
4.138AspGly: 4.138 ± 0.571
0.884AspHis: 0.884 ± 0.199
5.263AspIle: 5.263 ± 0.413
4.138AspLys: 4.138 ± 0.394
5.946AspLeu: 5.946 ± 0.562
2.009AspMet: 2.009 ± 0.29
3.816AspAsn: 3.816 ± 0.428
2.692AspPro: 2.692 ± 0.378
1.366AspGln: 1.366 ± 0.254
2.571AspArg: 2.571 ± 0.333
4.138AspSer: 4.138 ± 0.428
3.696AspThr: 3.696 ± 0.416
3.495AspVal: 3.495 ± 0.35
0.723AspTrp: 0.723 ± 0.144
3.134AspTyr: 3.134 ± 0.423
0.0AspXaa: 0.0 ± 0.0
Glu
6.066GluAla: 6.066 ± 0.551
0.924GluCys: 0.924 ± 0.198
4.017GluAsp: 4.017 ± 0.416
5.664GluGlu: 5.664 ± 0.586
3.174GluPhe: 3.174 ± 0.387
3.656GluGly: 3.656 ± 0.432
1.406GluHis: 1.406 ± 0.246
5.182GluIle: 5.182 ± 0.455
4.299GluLys: 4.299 ± 0.49
7.432GluLeu: 7.432 ± 0.621
1.848GluMet: 1.848 ± 0.31
2.852GluAsn: 2.852 ± 0.312
1.768GluPro: 1.768 ± 0.467
3.174GluGln: 3.174 ± 0.412
3.334GluArg: 3.334 ± 0.322
4.459GluSer: 4.459 ± 0.406
3.013GluThr: 3.013 ± 0.364
4.941GluVal: 4.941 ± 0.511
0.844GluTrp: 0.844 ± 0.193
3.214GluTyr: 3.214 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
3.013PheAla: 3.013 ± 0.348
0.402PheCys: 0.402 ± 0.144
2.611PheAsp: 2.611 ± 0.389
2.772PheGlu: 2.772 ± 0.36
1.406PhePhe: 1.406 ± 0.264
3.093PheGly: 3.093 ± 0.37
0.964PheHis: 0.964 ± 0.213
2.451PheIle: 2.451 ± 0.264
2.812PheLys: 2.812 ± 0.34
3.415PheLeu: 3.415 ± 0.319
0.964PheMet: 0.964 ± 0.182
2.531PheAsn: 2.531 ± 0.272
1.727PhePro: 1.727 ± 0.246
0.924PheGln: 0.924 ± 0.252
1.486PheArg: 1.486 ± 0.263
2.451PheSer: 2.451 ± 0.348
3.294PheThr: 3.294 ± 0.299
2.25PheVal: 2.25 ± 0.33
0.362PheTrp: 0.362 ± 0.107
1.165PheTyr: 1.165 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
4.178GlyAla: 4.178 ± 0.439
1.125GlyCys: 1.125 ± 0.244
4.459GlyAsp: 4.459 ± 0.482
4.379GlyGlu: 4.379 ± 0.453
2.933GlyPhe: 2.933 ± 0.375
3.214GlyGly: 3.214 ± 0.397
1.004GlyHis: 1.004 ± 0.207
4.821GlyIle: 4.821 ± 0.395
5.745GlyLys: 5.745 ± 0.637
4.74GlyLeu: 4.74 ± 0.444
1.768GlyMet: 1.768 ± 0.341
4.138GlyAsn: 4.138 ± 0.363
0.683GlyPro: 0.683 ± 0.2
1.607GlyGln: 1.607 ± 0.251
2.692GlyArg: 2.692 ± 0.342
4.379GlySer: 4.379 ± 0.547
4.419GlyThr: 4.419 ± 0.461
4.058GlyVal: 4.058 ± 0.353
0.924GlyTrp: 0.924 ± 0.163
2.571GlyTyr: 2.571 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
1.245HisAla: 1.245 ± 0.216
0.362HisCys: 0.362 ± 0.142
1.045HisAsp: 1.045 ± 0.226
0.844HisGlu: 0.844 ± 0.169
0.924HisPhe: 0.924 ± 0.2
1.286HisGly: 1.286 ± 0.192
0.562HisHis: 0.562 ± 0.144
1.406HisIle: 1.406 ± 0.232
1.768HisLys: 1.768 ± 0.282
1.085HisLeu: 1.085 ± 0.205
0.643HisMet: 0.643 ± 0.164
0.924HisAsn: 0.924 ± 0.192
0.683HisPro: 0.683 ± 0.176
0.562HisGln: 0.562 ± 0.142
1.085HisArg: 1.085 ± 0.201
1.446HisSer: 1.446 ± 0.263
0.844HisThr: 0.844 ± 0.159
1.165HisVal: 1.165 ± 0.208
0.161HisTrp: 0.161 ± 0.085
0.723HisTyr: 0.723 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.419IleAla: 4.419 ± 0.564
0.482IleCys: 0.482 ± 0.134
4.62IleAsp: 4.62 ± 0.491
5.102IleGlu: 5.102 ± 0.411
2.571IlePhe: 2.571 ± 0.377
3.816IleGly: 3.816 ± 0.348
0.964IleHis: 0.964 ± 0.197
4.098IleIle: 4.098 ± 0.484
4.058IleLys: 4.058 ± 0.551
5.825IleLeu: 5.825 ± 0.477
1.567IleMet: 1.567 ± 0.293
3.575IleAsn: 3.575 ± 0.277
3.616IlePro: 3.616 ± 0.418
2.611IleGln: 2.611 ± 0.311
3.174IleArg: 3.174 ± 0.394
4.821IleSer: 4.821 ± 0.438
4.499IleThr: 4.499 ± 0.398
4.138IleVal: 4.138 ± 0.383
0.562IleTrp: 0.562 ± 0.142
2.611IleTyr: 2.611 ± 0.312
0.0IleXaa: 0.0 ± 0.0
Lys
5.584LysAla: 5.584 ± 0.591
0.482LysCys: 0.482 ± 0.137
4.459LysAsp: 4.459 ± 0.331
6.227LysGlu: 6.227 ± 0.525
2.973LysPhe: 2.973 ± 0.325
4.499LysGly: 4.499 ± 0.406
1.366LysHis: 1.366 ± 0.256
4.017LysIle: 4.017 ± 0.433
4.098LysLys: 4.098 ± 0.508
7.03LysLeu: 7.03 ± 0.64
2.129LysMet: 2.129 ± 0.306
1.527LysAsn: 1.527 ± 0.237
2.892LysPro: 2.892 ± 0.351
2.129LysGln: 2.129 ± 0.381
2.812LysArg: 2.812 ± 0.342
4.299LysSer: 4.299 ± 0.429
3.696LysThr: 3.696 ± 0.421
5.383LysVal: 5.383 ± 0.549
0.803LysTrp: 0.803 ± 0.217
2.772LysTyr: 2.772 ± 0.344
0.0LysXaa: 0.0 ± 0.0
Leu
6.026LeuAla: 6.026 ± 0.626
0.603LeuCys: 0.603 ± 0.164
7.713LeuAsp: 7.713 ± 0.645
7.392LeuGlu: 7.392 ± 0.564
2.37LeuPhe: 2.37 ± 0.305
5.705LeuGly: 5.705 ± 0.449
1.647LeuHis: 1.647 ± 0.271
5.062LeuIle: 5.062 ± 0.456
5.785LeuLys: 5.785 ± 0.416
7.071LeuLeu: 7.071 ± 0.695
1.928LeuMet: 1.928 ± 0.3
4.861LeuAsn: 4.861 ± 0.378
4.299LeuPro: 4.299 ± 0.462
2.852LeuGln: 2.852 ± 0.29
3.816LeuArg: 3.816 ± 0.412
6.468LeuSer: 6.468 ± 0.516
5.865LeuThr: 5.865 ± 0.54
5.946LeuVal: 5.946 ± 0.5
0.643LeuTrp: 0.643 ± 0.16
2.41LeuTyr: 2.41 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.009MetAla: 2.009 ± 0.365
0.362MetCys: 0.362 ± 0.117
1.486MetAsp: 1.486 ± 0.243
2.049MetGlu: 2.049 ± 0.275
0.763MetPhe: 0.763 ± 0.189
1.205MetGly: 1.205 ± 0.224
0.482MetHis: 0.482 ± 0.138
1.607MetIle: 1.607 ± 0.293
2.009MetLys: 2.009 ± 0.294
2.651MetLeu: 2.651 ± 0.294
0.562MetMet: 0.562 ± 0.135
0.924MetAsn: 0.924 ± 0.189
0.964MetPro: 0.964 ± 0.209
1.125MetGln: 1.125 ± 0.281
0.924MetArg: 0.924 ± 0.149
2.049MetSer: 2.049 ± 0.261
1.326MetThr: 1.326 ± 0.255
1.205MetVal: 1.205 ± 0.243
0.281MetTrp: 0.281 ± 0.112
0.964MetTyr: 0.964 ± 0.198
0.0MetXaa: 0.0 ± 0.0
Asn
3.897AsnAla: 3.897 ± 0.635
0.402AsnCys: 0.402 ± 0.126
2.651AsnAsp: 2.651 ± 0.331
3.013AsnGlu: 3.013 ± 0.3
1.808AsnPhe: 1.808 ± 0.278
3.415AsnGly: 3.415 ± 0.496
0.803AsnHis: 0.803 ± 0.162
3.937AsnIle: 3.937 ± 0.348
3.736AsnLys: 3.736 ± 0.371
4.339AsnLeu: 4.339 ± 0.412
1.326AsnMet: 1.326 ± 0.218
3.214AsnAsn: 3.214 ± 0.439
2.973AsnPro: 2.973 ± 0.309
1.406AsnGln: 1.406 ± 0.269
2.049AsnArg: 2.049 ± 0.257
3.977AsnSer: 3.977 ± 0.589
3.495AsnThr: 3.495 ± 0.438
2.973AsnVal: 2.973 ± 0.312
0.562AsnTrp: 0.562 ± 0.128
2.491AsnTyr: 2.491 ± 0.306
0.0AsnXaa: 0.0 ± 0.0
Pro
2.25ProAla: 2.25 ± 0.309
0.402ProCys: 0.402 ± 0.13
2.169ProAsp: 2.169 ± 0.307
4.138ProGlu: 4.138 ± 0.554
1.446ProPhe: 1.446 ± 0.215
2.451ProGly: 2.451 ± 0.395
0.643ProHis: 0.643 ± 0.188
2.611ProIle: 2.611 ± 0.312
2.491ProLys: 2.491 ± 0.343
3.053ProLeu: 3.053 ± 0.369
0.522ProMet: 0.522 ± 0.135
2.29ProAsn: 2.29 ± 0.293
1.125ProPro: 1.125 ± 0.26
1.205ProGln: 1.205 ± 0.246
1.326ProArg: 1.326 ± 0.218
2.41ProSer: 2.41 ± 0.35
2.009ProThr: 2.009 ± 0.318
3.415ProVal: 3.415 ± 0.405
0.402ProTrp: 0.402 ± 0.145
1.406ProTyr: 1.406 ± 0.211
0.0ProXaa: 0.0 ± 0.0
Gln
2.772GlnAla: 2.772 ± 0.427
0.281GlnCys: 0.281 ± 0.11
1.687GlnAsp: 1.687 ± 0.268
1.969GlnGlu: 1.969 ± 0.309
1.366GlnPhe: 1.366 ± 0.248
1.727GlnGly: 1.727 ± 0.256
0.522GlnHis: 0.522 ± 0.143
2.049GlnIle: 2.049 ± 0.329
2.049GlnLys: 2.049 ± 0.33
3.495GlnLeu: 3.495 ± 0.554
0.924GlnMet: 0.924 ± 0.2
1.165GlnAsn: 1.165 ± 0.212
0.924GlnPro: 0.924 ± 0.171
1.326GlnGln: 1.326 ± 0.281
1.567GlnArg: 1.567 ± 0.249
1.888GlnSer: 1.888 ± 0.281
1.848GlnThr: 1.848 ± 0.383
2.892GlnVal: 2.892 ± 0.603
0.321GlnTrp: 0.321 ± 0.122
1.647GlnTyr: 1.647 ± 0.262
0.0GlnXaa: 0.0 ± 0.0
Arg
3.134ArgAla: 3.134 ± 0.371
0.281ArgCys: 0.281 ± 0.095
3.174ArgAsp: 3.174 ± 0.296
3.415ArgGlu: 3.415 ± 0.431
1.928ArgPhe: 1.928 ± 0.242
2.772ArgGly: 2.772 ± 0.368
0.522ArgHis: 0.522 ± 0.159
3.093ArgIle: 3.093 ± 0.363
2.692ArgLys: 2.692 ± 0.328
3.495ArgLeu: 3.495 ± 0.332
1.446ArgMet: 1.446 ± 0.229
2.049ArgAsn: 2.049 ± 0.264
1.366ArgPro: 1.366 ± 0.247
1.326ArgGln: 1.326 ± 0.198
2.169ArgArg: 2.169 ± 0.467
3.254ArgSer: 3.254 ± 0.389
2.37ArgThr: 2.37 ± 0.312
3.375ArgVal: 3.375 ± 0.33
0.402ArgTrp: 0.402 ± 0.124
1.245ArgTyr: 1.245 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
5.986SerAla: 5.986 ± 0.625
0.683SerCys: 0.683 ± 0.178
4.258SerAsp: 4.258 ± 0.556
4.499SerGlu: 4.499 ± 0.408
2.611SerPhe: 2.611 ± 0.359
5.423SerGly: 5.423 ± 0.623
1.326SerHis: 1.326 ± 0.218
4.58SerIle: 4.58 ± 0.318
4.941SerLys: 4.941 ± 0.472
5.705SerLeu: 5.705 ± 0.466
1.326SerMet: 1.326 ± 0.209
3.897SerAsn: 3.897 ± 0.528
2.25SerPro: 2.25 ± 0.259
2.089SerGln: 2.089 ± 0.459
2.692SerArg: 2.692 ± 0.388
5.745SerSer: 5.745 ± 0.491
4.7SerThr: 4.7 ± 0.478
4.379SerVal: 4.379 ± 0.44
0.924SerTrp: 0.924 ± 0.194
2.049SerTyr: 2.049 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
3.696ThrAla: 3.696 ± 0.376
0.603ThrCys: 0.603 ± 0.145
3.535ThrAsp: 3.535 ± 0.383
3.375ThrGlu: 3.375 ± 0.377
2.21ThrPhe: 2.21 ± 0.325
5.022ThrGly: 5.022 ± 0.467
1.165ThrHis: 1.165 ± 0.195
4.459ThrIle: 4.459 ± 0.46
4.058ThrLys: 4.058 ± 0.406
5.906ThrLeu: 5.906 ± 0.648
1.085ThrMet: 1.085 ± 0.205
3.776ThrAsn: 3.776 ± 0.45
2.571ThrPro: 2.571 ± 0.299
2.089ThrGln: 2.089 ± 0.333
2.933ThrArg: 2.933 ± 0.373
3.535ThrSer: 3.535 ± 0.461
3.937ThrThr: 3.937 ± 0.429
3.857ThrVal: 3.857 ± 0.374
0.442ThrTrp: 0.442 ± 0.127
2.33ThrTyr: 2.33 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
4.821ValAla: 4.821 ± 0.419
0.562ValCys: 0.562 ± 0.141
5.062ValAsp: 5.062 ± 0.426
4.54ValGlu: 4.54 ± 0.424
3.013ValPhe: 3.013 ± 0.38
3.937ValGly: 3.937 ± 0.412
1.245ValHis: 1.245 ± 0.26
3.897ValIle: 3.897 ± 0.409
3.375ValLys: 3.375 ± 0.384
4.821ValLeu: 4.821 ± 0.444
1.768ValMet: 1.768 ± 0.279
3.334ValAsn: 3.334 ± 0.3
3.053ValPro: 3.053 ± 0.421
1.848ValGln: 1.848 ± 0.283
3.093ValArg: 3.093 ± 0.377
5.383ValSer: 5.383 ± 0.441
3.736ValThr: 3.736 ± 0.43
4.7ValVal: 4.7 ± 0.559
0.562ValTrp: 0.562 ± 0.139
3.053ValTyr: 3.053 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
0.643TrpAla: 0.643 ± 0.17
0.08TrpCys: 0.08 ± 0.053
0.482TrpAsp: 0.482 ± 0.159
1.125TrpGlu: 1.125 ± 0.193
0.281TrpPhe: 0.281 ± 0.103
0.803TrpGly: 0.803 ± 0.215
0.201TrpHis: 0.201 ± 0.082
0.482TrpIle: 0.482 ± 0.129
0.643TrpLys: 0.643 ± 0.195
1.366TrpLeu: 1.366 ± 0.273
0.241TrpMet: 0.241 ± 0.105
0.723TrpAsn: 0.723 ± 0.164
0.04TrpPro: 0.04 ± 0.037
0.321TrpGln: 0.321 ± 0.108
0.482TrpArg: 0.482 ± 0.132
0.723TrpSer: 0.723 ± 0.212
0.562TrpThr: 0.562 ± 0.172
0.763TrpVal: 0.763 ± 0.175
0.121TrpTrp: 0.121 ± 0.076
0.522TrpTyr: 0.522 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.571TyrAla: 2.571 ± 0.339
0.603TyrCys: 0.603 ± 0.148
2.772TyrAsp: 2.772 ± 0.4
2.41TyrGlu: 2.41 ± 0.278
1.486TyrPhe: 1.486 ± 0.271
2.009TyrGly: 2.009 ± 0.283
1.286TyrHis: 1.286 ± 0.243
2.37TyrIle: 2.37 ± 0.398
2.651TyrLys: 2.651 ± 0.288
3.375TyrLeu: 3.375 ± 0.337
0.924TyrMet: 0.924 ± 0.183
1.888TyrAsn: 1.888 ± 0.205
1.607TyrPro: 1.607 ± 0.249
1.245TyrGln: 1.245 ± 0.189
1.768TyrArg: 1.768 ± 0.298
2.812TyrSer: 2.812 ± 0.399
2.21TyrThr: 2.21 ± 0.288
2.451TyrVal: 2.451 ± 0.304
0.763TyrTrp: 0.763 ± 0.154
1.848TyrTyr: 1.848 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (24893 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski