Streptococcus satellite phage Javan209

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFT5|A0A4D5ZFT5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan209 OX=2558569 GN=JavanS209_0012 PE=4 SV=1
MM1 pKa = 7.27NRR3 pKa = 11.84LKK5 pKa = 10.53EE6 pKa = 3.96LRR8 pKa = 11.84KK9 pKa = 10.17EE10 pKa = 4.05KK11 pKa = 10.72GLSQQALANEE21 pKa = 4.52LGVHH25 pKa = 5.94YY26 pKa = 8.0RR27 pKa = 11.84TLQNWEE33 pKa = 4.02NGEE36 pKa = 4.17TSIKK40 pKa = 8.86PAKK43 pKa = 10.41AEE45 pKa = 3.85EE46 pKa = 4.04LAKK49 pKa = 10.82KK50 pKa = 10.23FGVNIGYY57 pKa = 9.89LLGYY61 pKa = 9.13DD62 pKa = 3.45EE63 pKa = 5.69KK64 pKa = 10.77PYY66 pKa = 10.96KK67 pKa = 10.05EE68 pKa = 3.72LSEE71 pKa = 4.26FVKK74 pKa = 10.38EE75 pKa = 4.07LQKK78 pKa = 11.43EE79 pKa = 4.05EE80 pKa = 4.86LEE82 pKa = 5.06DD83 pKa = 3.73IFDD86 pKa = 4.22SEE88 pKa = 4.19IDD90 pKa = 3.91SIFMDD95 pKa = 5.71FIDD98 pKa = 5.68FLYY101 pKa = 11.07QNDD104 pKa = 3.51FHH106 pKa = 9.26ISDD109 pKa = 3.99NKK111 pKa = 10.43ILLLFNTLLAVDD123 pKa = 4.48KK124 pKa = 11.44NKK126 pKa = 10.62GDD128 pKa = 3.41NFHH131 pKa = 5.88YY132 pKa = 10.33QRR134 pKa = 11.84YY135 pKa = 6.45TNPKK139 pKa = 8.77NSYY142 pKa = 10.71SLMDD146 pKa = 3.77EE147 pKa = 4.12AQEE150 pKa = 4.01KK151 pKa = 10.98HH152 pKa = 5.14EE153 pKa = 4.31TSSDD157 pKa = 3.28KK158 pKa = 11.38

Molecular weight: 18.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZIA2|A0A4D5ZIA2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan209 OX=2558569 GN=JavanS209_0004 PE=4 SV=1
MM1 pKa = 7.75QEE3 pKa = 3.57EE4 pKa = 4.72KK5 pKa = 10.36KK6 pKa = 10.44RR7 pKa = 11.84SRR9 pKa = 11.84GRR11 pKa = 11.84PATGLKK17 pKa = 9.92RR18 pKa = 11.84NKK20 pKa = 9.86KK21 pKa = 8.73IAINFSQEE29 pKa = 3.45EE30 pKa = 3.66VDD32 pKa = 4.15YY33 pKa = 10.84IKK35 pKa = 11.16SKK37 pKa = 11.25AKK39 pKa = 10.75DD40 pKa = 3.41NGLSIPNLILKK51 pKa = 9.57ALKK54 pKa = 10.46KK55 pKa = 10.54LL56 pKa = 3.65

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2118

47

380

162.9

18.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.43 ± 0.6

0.614 ± 0.147

5.194 ± 0.759

8.451 ± 0.898

4.438 ± 0.426

4.438 ± 0.661

1.416 ± 0.376

7.46 ± 0.558

11.851 ± 0.658

9.632 ± 0.646

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.305

6.799 ± 0.472

2.55 ± 0.271

3.919 ± 0.445

3.588 ± 0.419

5.194 ± 0.461

5.477 ± 0.478

5.807 ± 0.625

0.661 ± 0.136

4.816 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski