Candidatus Marinamargulisbacteria bacterium SCGC AG-414-C22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328RF38|A0A328RF38_9BACT Uncharacterized protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-414-C22 OX=2184344 GN=DID76_00170 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.32FKK4 pKa = 11.01LFFIIFTITLLVGCSSSEE22 pKa = 3.74VSTEE26 pKa = 3.38NDD28 pKa = 3.1TVYY31 pKa = 11.22EE32 pKa = 3.91NLTFSGHH39 pKa = 4.3VAIDD43 pKa = 3.67ASSGTSSLEE52 pKa = 3.54ASAYY56 pKa = 10.65ALLSNSNAPSTDD68 pKa = 2.74EE69 pKa = 4.11SNYY72 pKa = 10.14MIVIQNQISNRR83 pKa = 11.84LYY85 pKa = 10.89LSQLDD90 pKa = 3.74ADD92 pKa = 4.42GTFHH96 pKa = 7.62FSDD99 pKa = 3.98NGTDD103 pKa = 3.32AQAIQSVNHH112 pKa = 6.62DD113 pKa = 3.45GGYY116 pKa = 10.54GNHH119 pKa = 5.9FMVMLLQKK127 pKa = 10.92DD128 pKa = 3.75PLEE131 pKa = 4.63VKK133 pKa = 10.43AVAYY137 pKa = 10.55LPGTSEE143 pKa = 3.72EE144 pKa = 5.1GYY146 pKa = 10.76SGFQFSEE153 pKa = 4.37SFDD156 pKa = 3.56QEE158 pKa = 3.99LQFNYY163 pKa = 10.59NSDD166 pKa = 3.96SASMTVSEE174 pKa = 4.21THH176 pKa = 6.61INDD179 pKa = 3.28IAGLQVDD186 pKa = 3.69EE187 pKa = 5.15DD188 pKa = 4.27FVVRR192 pKa = 11.84LVNDD196 pKa = 4.34TPAGATNFGKK206 pKa = 8.6STDD209 pKa = 3.79SQTSTVSDD217 pKa = 3.76INQLDD222 pKa = 4.16PDD224 pKa = 3.81QDD226 pKa = 3.73GQPNIFDD233 pKa = 3.82GMNNGSDD240 pKa = 4.4LDD242 pKa = 4.15NLSADD247 pKa = 3.83NKK249 pKa = 10.2TEE251 pKa = 3.86GATVSDD257 pKa = 5.01SIASSIMFMNLKK269 pKa = 9.73IDD271 pKa = 4.47LEE273 pKa = 4.75DD274 pKa = 3.47STSFTVTDD282 pKa = 3.34NAIIVLEE289 pKa = 4.13VIPRR293 pKa = 11.84SSASIHH299 pKa = 5.91SIRR302 pKa = 11.84VAEE305 pKa = 4.29LVTPSSSSIRR315 pKa = 11.84LLHH318 pKa = 6.21TNYY321 pKa = 9.75QQSLMEE327 pKa = 4.37KK328 pKa = 9.41LPNGFTEE335 pKa = 4.09VDD337 pKa = 3.61SYY339 pKa = 10.23PTEE342 pKa = 4.35GSLWSADD349 pKa = 3.96DD350 pKa = 3.72YY351 pKa = 11.91QLYY354 pKa = 9.5QALNQDD360 pKa = 3.07GDD362 pKa = 4.19TVYY365 pKa = 10.24TALIKK370 pKa = 10.6PNNNQFEE377 pKa = 4.42PGNLILLEE385 pKa = 4.2VNLTDD390 pKa = 4.68GSKK393 pKa = 10.34EE394 pKa = 3.95YY395 pKa = 10.95YY396 pKa = 10.22FNSINFKK403 pKa = 10.38FQTIPTDD410 pKa = 3.45QTNWTHH416 pKa = 6.36GGNGSTASPYY426 pKa = 10.84NILDD430 pKa = 3.1TGGRR434 pKa = 11.84VFTWDD439 pKa = 3.37HH440 pKa = 6.51PLDD443 pKa = 3.87EE444 pKa = 5.61FGTEE448 pKa = 4.4LEE450 pKa = 4.08GLNYY454 pKa = 9.92QFEE457 pKa = 4.65LFYY460 pKa = 10.83YY461 pKa = 8.88TDD463 pKa = 3.41NSGTCDD469 pKa = 3.43PHH471 pKa = 8.01VDD473 pKa = 3.8YY474 pKa = 11.27QIGTMQIIDD483 pKa = 3.91FRR485 pKa = 11.84VSDD488 pKa = 4.02LANYY492 pKa = 10.37NDD494 pKa = 3.46ISEE497 pKa = 4.46TEE499 pKa = 3.56IDD501 pKa = 3.86FAGSTTQCLQLDD513 pKa = 3.9IAGSYY518 pKa = 10.22PYY520 pKa = 10.8GDD522 pKa = 3.33NAALKK527 pKa = 10.02IYY529 pKa = 10.37LKK531 pKa = 10.59RR532 pKa = 11.84NSWW535 pKa = 3.2

Molecular weight: 58.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328RGJ9|A0A328RGJ9_9BACT Uncharacterized protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-414-C22 OX=2184344 GN=DID76_03085 PE=4 SV=1
MM1 pKa = 7.38LRR3 pKa = 11.84SHH5 pKa = 7.07CFASLRR11 pKa = 11.84FALLCIASLRR21 pKa = 11.84IALHH25 pKa = 6.33RR26 pKa = 11.84FAALLCIALLCIALHH41 pKa = 6.45RR42 pKa = 11.84FAALLCIASRR52 pKa = 11.84CFALLRR58 pKa = 11.84FARR61 pKa = 11.84IALFDD66 pKa = 3.99WLSHH70 pKa = 5.55KK71 pKa = 10.71QSFVV75 pKa = 3.02

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

930

0

930

325261

46

5061

349.7

39.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.805 ± 0.087

1.301 ± 0.036

5.623 ± 0.083

5.659 ± 0.096

5.215 ± 0.08

5.278 ± 0.1

2.604 ± 0.045

8.32 ± 0.094

7.786 ± 0.105

10.298 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.034

5.612 ± 0.082

3.502 ± 0.042

4.26 ± 0.062

3.174 ± 0.058

7.072 ± 0.081

6.035 ± 0.07

5.784 ± 0.079

0.818 ± 0.027

3.803 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski