Amino acid dipepetide frequency for Mycobacterium phage Michley

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.514AlaAla: 13.514 ± 1.524
0.81AlaCys: 0.81 ± 0.221
7.162AlaAsp: 7.162 ± 0.739
6.539AlaGlu: 6.539 ± 0.723
3.363AlaPhe: 3.363 ± 0.48
7.66AlaGly: 7.66 ± 0.823
1.432AlaHis: 1.432 ± 0.329
4.359AlaIle: 4.359 ± 0.496
4.173AlaLys: 4.173 ± 0.559
8.906AlaLeu: 8.906 ± 0.696
2.803AlaMet: 2.803 ± 0.457
2.989AlaAsn: 2.989 ± 0.414
4.609AlaPro: 4.609 ± 0.792
2.865AlaGln: 2.865 ± 0.496
6.352AlaArg: 6.352 ± 0.518
4.795AlaSer: 4.795 ± 0.632
5.73AlaThr: 5.73 ± 0.666
9.404AlaVal: 9.404 ± 0.851
1.931AlaTrp: 1.931 ± 0.355
2.865AlaTyr: 2.865 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.934CysAla: 0.934 ± 0.27
0.0CysCys: 0.0 ± 0.0
0.685CysAsp: 0.685 ± 0.218
0.623CysGlu: 0.623 ± 0.199
0.125CysPhe: 0.125 ± 0.082
0.498CysGly: 0.498 ± 0.188
0.187CysHis: 0.187 ± 0.115
0.311CysIle: 0.311 ± 0.126
0.249CysLys: 0.249 ± 0.124
0.498CysLeu: 0.498 ± 0.205
0.125CysMet: 0.125 ± 0.097
0.249CysAsn: 0.249 ± 0.126
0.374CysPro: 0.374 ± 0.183
0.125CysGln: 0.125 ± 0.089
0.374CysArg: 0.374 ± 0.163
0.498CysSer: 0.498 ± 0.207
0.311CysThr: 0.311 ± 0.136
0.249CysVal: 0.249 ± 0.112
0.249CysTrp: 0.249 ± 0.109
0.062CysTyr: 0.062 ± 0.065
0.0CysXaa: 0.0 ± 0.0
Asp
7.1AspAla: 7.1 ± 0.706
0.498AspCys: 0.498 ± 0.187
4.484AspAsp: 4.484 ± 0.539
3.861AspGlu: 3.861 ± 0.532
2.429AspPhe: 2.429 ± 0.403
5.854AspGly: 5.854 ± 0.543
1.183AspHis: 1.183 ± 0.289
2.553AspIle: 2.553 ± 0.378
2.678AspLys: 2.678 ± 0.404
7.1AspLeu: 7.1 ± 0.772
1.059AspMet: 1.059 ± 0.184
1.931AspAsn: 1.931 ± 0.33
4.795AspPro: 4.795 ± 0.601
2.055AspGln: 2.055 ± 0.439
3.612AspArg: 3.612 ± 0.385
3.114AspSer: 3.114 ± 0.428
3.737AspThr: 3.737 ± 0.49
4.048AspVal: 4.048 ± 0.401
1.682AspTrp: 1.682 ± 0.337
2.055AspTyr: 2.055 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
5.605GluAla: 5.605 ± 0.813
0.311GluCys: 0.311 ± 0.163
4.546GluAsp: 4.546 ± 0.609
4.982GluGlu: 4.982 ± 0.553
2.242GluPhe: 2.242 ± 0.345
3.674GluGly: 3.674 ± 0.34
1.37GluHis: 1.37 ± 0.345
3.363GluIle: 3.363 ± 0.444
2.927GluLys: 2.927 ± 0.498
6.913GluLeu: 6.913 ± 0.658
1.557GluMet: 1.557 ± 0.271
1.432GluAsn: 1.432 ± 0.363
2.491GluPro: 2.491 ± 0.449
2.74GluGln: 2.74 ± 0.378
4.048GluArg: 4.048 ± 0.582
3.674GluSer: 3.674 ± 0.453
3.986GluThr: 3.986 ± 0.518
5.605GluVal: 5.605 ± 0.577
1.993GluTrp: 1.993 ± 0.41
2.927GluTyr: 2.927 ± 0.467
0.0GluXaa: 0.0 ± 0.0
Phe
2.553PheAla: 2.553 ± 0.242
0.311PheCys: 0.311 ± 0.163
2.803PheAsp: 2.803 ± 0.383
1.931PheGlu: 1.931 ± 0.349
0.561PhePhe: 0.561 ± 0.171
3.799PheGly: 3.799 ± 0.509
0.623PheHis: 0.623 ± 0.228
1.37PheIle: 1.37 ± 0.268
1.246PheLys: 1.246 ± 0.267
2.367PheLeu: 2.367 ± 0.408
0.498PheMet: 0.498 ± 0.183
0.872PheAsn: 0.872 ± 0.243
1.682PhePro: 1.682 ± 0.318
0.872PheGln: 0.872 ± 0.21
1.931PheArg: 1.931 ± 0.349
1.868PheSer: 1.868 ± 0.464
1.806PheThr: 1.806 ± 0.353
1.993PheVal: 1.993 ± 0.406
0.561PheTrp: 0.561 ± 0.175
0.872PheTyr: 0.872 ± 0.26
0.0PheXaa: 0.0 ± 0.0
Gly
6.975GlyAla: 6.975 ± 1.037
0.685GlyCys: 0.685 ± 0.246
5.48GlyAsp: 5.48 ± 0.472
4.422GlyGlu: 4.422 ± 0.477
2.927GlyPhe: 2.927 ± 0.456
9.093GlyGly: 9.093 ± 2.112
1.868GlyHis: 1.868 ± 0.39
3.986GlyIle: 3.986 ± 0.606
3.363GlyLys: 3.363 ± 0.522
7.722GlyLeu: 7.722 ± 0.901
2.055GlyMet: 2.055 ± 0.32
3.488GlyAsn: 3.488 ± 0.424
3.737GlyPro: 3.737 ± 0.596
2.678GlyGln: 2.678 ± 0.347
4.359GlyArg: 4.359 ± 0.484
6.477GlySer: 6.477 ± 0.93
5.667GlyThr: 5.667 ± 0.726
5.107GlyVal: 5.107 ± 0.557
2.616GlyTrp: 2.616 ± 0.351
2.74GlyTyr: 2.74 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
1.744HisAla: 1.744 ± 0.316
0.125HisCys: 0.125 ± 0.094
1.121HisAsp: 1.121 ± 0.249
1.432HisGlu: 1.432 ± 0.298
0.685HisPhe: 0.685 ± 0.201
1.682HisGly: 1.682 ± 0.387
0.561HisHis: 0.561 ± 0.195
1.059HisIle: 1.059 ± 0.228
1.121HisLys: 1.121 ± 0.284
1.432HisLeu: 1.432 ± 0.355
0.062HisMet: 0.062 ± 0.063
0.374HisAsn: 0.374 ± 0.135
1.37HisPro: 1.37 ± 0.242
0.747HisGln: 0.747 ± 0.224
1.495HisArg: 1.495 ± 0.292
0.436HisSer: 0.436 ± 0.145
1.121HisThr: 1.121 ± 0.335
1.495HisVal: 1.495 ± 0.337
0.436HisTrp: 0.436 ± 0.19
0.685HisTyr: 0.685 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
6.228IleAla: 6.228 ± 0.637
0.311IleCys: 0.311 ± 0.134
3.363IleAsp: 3.363 ± 0.439
3.986IleGlu: 3.986 ± 0.524
0.872IlePhe: 0.872 ± 0.205
3.488IleGly: 3.488 ± 0.478
0.747IleHis: 0.747 ± 0.21
1.619IleIle: 1.619 ± 0.372
1.682IleLys: 1.682 ± 0.298
3.924IleLeu: 3.924 ± 0.489
0.81IleMet: 0.81 ± 0.187
1.931IleAsn: 1.931 ± 0.333
2.927IlePro: 2.927 ± 0.418
1.37IleGln: 1.37 ± 0.391
3.799IleArg: 3.799 ± 0.446
3.612IleSer: 3.612 ± 0.493
3.238IleThr: 3.238 ± 0.411
2.74IleVal: 2.74 ± 0.479
0.623IleTrp: 0.623 ± 0.205
1.495IleTyr: 1.495 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
3.363LysAla: 3.363 ± 0.573
0.187LysCys: 0.187 ± 0.105
2.553LysAsp: 2.553 ± 0.405
2.18LysGlu: 2.18 ± 0.376
1.557LysPhe: 1.557 ± 0.295
2.429LysGly: 2.429 ± 0.365
1.37LysHis: 1.37 ± 0.296
2.803LysIle: 2.803 ± 0.501
1.931LysLys: 1.931 ± 0.442
3.425LysLeu: 3.425 ± 0.467
1.121LysMet: 1.121 ± 0.215
1.682LysAsn: 1.682 ± 0.289
2.553LysPro: 2.553 ± 0.439
1.495LysGln: 1.495 ± 0.311
2.491LysArg: 2.491 ± 0.411
2.304LysSer: 2.304 ± 0.384
2.367LysThr: 2.367 ± 0.42
3.176LysVal: 3.176 ± 0.529
0.934LysTrp: 0.934 ± 0.238
0.934LysTyr: 0.934 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
10.214LeuAla: 10.214 ± 0.875
0.436LeuCys: 0.436 ± 0.164
5.979LeuAsp: 5.979 ± 0.552
5.418LeuGlu: 5.418 ± 0.571
1.931LeuPhe: 1.931 ± 0.431
7.411LeuGly: 7.411 ± 0.838
1.619LeuHis: 1.619 ± 0.339
4.795LeuIle: 4.795 ± 0.586
3.861LeuLys: 3.861 ± 0.51
5.667LeuLeu: 5.667 ± 0.575
1.682LeuMet: 1.682 ± 0.278
3.114LeuAsn: 3.114 ± 0.448
5.169LeuPro: 5.169 ± 0.537
2.491LeuGln: 2.491 ± 0.405
6.352LeuArg: 6.352 ± 0.56
5.418LeuSer: 5.418 ± 0.545
6.415LeuThr: 6.415 ± 0.517
5.169LeuVal: 5.169 ± 0.755
1.183LeuTrp: 1.183 ± 0.301
2.242LeuTyr: 2.242 ± 0.416
0.0LeuXaa: 0.0 ± 0.0
Met
2.553MetAla: 2.553 ± 0.374
0.0MetCys: 0.0 ± 0.0
1.183MetAsp: 1.183 ± 0.229
1.682MetGlu: 1.682 ± 0.317
0.374MetPhe: 0.374 ± 0.153
1.557MetGly: 1.557 ± 0.313
0.249MetHis: 0.249 ± 0.122
0.872MetIle: 0.872 ± 0.223
0.996MetLys: 0.996 ± 0.242
1.308MetLeu: 1.308 ± 0.262
0.187MetMet: 0.187 ± 0.091
1.121MetAsn: 1.121 ± 0.221
1.121MetPro: 1.121 ± 0.254
0.561MetGln: 0.561 ± 0.156
1.183MetArg: 1.183 ± 0.257
2.117MetSer: 2.117 ± 0.365
2.055MetThr: 2.055 ± 0.242
1.495MetVal: 1.495 ± 0.342
0.374MetTrp: 0.374 ± 0.14
0.436MetTyr: 0.436 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
3.176AsnAla: 3.176 ± 0.533
0.062AsnCys: 0.062 ± 0.063
2.117AsnAsp: 2.117 ± 0.36
1.993AsnGlu: 1.993 ± 0.363
0.81AsnPhe: 0.81 ± 0.221
3.799AsnGly: 3.799 ± 0.521
0.623AsnHis: 0.623 ± 0.187
1.308AsnIle: 1.308 ± 0.295
0.747AsnLys: 0.747 ± 0.196
2.429AsnLeu: 2.429 ± 0.351
0.685AsnMet: 0.685 ± 0.172
1.121AsnAsn: 1.121 ± 0.28
2.553AsnPro: 2.553 ± 0.342
1.121AsnGln: 1.121 ± 0.299
1.682AsnArg: 1.682 ± 0.322
1.868AsnSer: 1.868 ± 0.368
2.055AsnThr: 2.055 ± 0.392
2.678AsnVal: 2.678 ± 0.409
0.685AsnTrp: 0.685 ± 0.193
1.308AsnTyr: 1.308 ± 0.271
0.0AsnXaa: 0.0 ± 0.0
Pro
5.231ProAla: 5.231 ± 0.547
0.374ProCys: 0.374 ± 0.15
4.235ProAsp: 4.235 ± 0.457
4.048ProGlu: 4.048 ± 0.551
1.931ProPhe: 1.931 ± 0.345
4.609ProGly: 4.609 ± 0.599
0.81ProHis: 0.81 ± 0.21
2.304ProIle: 2.304 ± 0.358
1.931ProLys: 1.931 ± 0.315
4.359ProLeu: 4.359 ± 0.586
0.934ProMet: 0.934 ± 0.227
1.619ProAsn: 1.619 ± 0.36
2.553ProPro: 2.553 ± 0.401
1.432ProGln: 1.432 ± 0.344
2.927ProArg: 2.927 ± 0.432
3.861ProSer: 3.861 ± 0.493
4.11ProThr: 4.11 ± 0.504
3.55ProVal: 3.55 ± 0.504
0.623ProTrp: 0.623 ± 0.269
1.495ProTyr: 1.495 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
3.114GlnAla: 3.114 ± 0.517
0.062GlnCys: 0.062 ± 0.063
1.682GlnAsp: 1.682 ± 0.416
1.432GlnGlu: 1.432 ± 0.302
1.183GlnPhe: 1.183 ± 0.247
2.429GlnGly: 2.429 ± 0.347
0.623GlnHis: 0.623 ± 0.192
2.616GlnIle: 2.616 ± 0.518
0.996GlnLys: 0.996 ± 0.224
3.612GlnLeu: 3.612 ± 0.423
0.934GlnMet: 0.934 ± 0.274
0.623GlnAsn: 0.623 ± 0.21
1.993GlnPro: 1.993 ± 0.369
1.868GlnGln: 1.868 ± 0.392
1.931GlnArg: 1.931 ± 0.417
1.557GlnSer: 1.557 ± 0.247
1.432GlnThr: 1.432 ± 0.252
2.616GlnVal: 2.616 ± 0.334
0.623GlnTrp: 0.623 ± 0.178
0.685GlnTyr: 0.685 ± 0.191
0.0GlnXaa: 0.0 ± 0.0
Arg
5.792ArgAla: 5.792 ± 0.661
0.81ArgCys: 0.81 ± 0.242
2.927ArgAsp: 2.927 ± 0.38
5.045ArgGlu: 5.045 ± 0.566
2.055ArgPhe: 2.055 ± 0.427
4.92ArgGly: 4.92 ± 0.552
0.934ArgHis: 0.934 ± 0.228
3.301ArgIle: 3.301 ± 0.551
3.114ArgLys: 3.114 ± 0.524
6.041ArgLeu: 6.041 ± 0.677
2.055ArgMet: 2.055 ± 0.367
2.367ArgAsn: 2.367 ± 0.466
2.055ArgPro: 2.055 ± 0.377
1.931ArgGln: 1.931 ± 0.354
5.294ArgArg: 5.294 ± 0.607
3.674ArgSer: 3.674 ± 0.49
2.989ArgThr: 2.989 ± 0.402
5.107ArgVal: 5.107 ± 0.614
1.183ArgTrp: 1.183 ± 0.291
1.868ArgTyr: 1.868 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
6.539SerAla: 6.539 ± 0.756
0.498SerCys: 0.498 ± 0.195
3.052SerAsp: 3.052 ± 0.449
3.924SerGlu: 3.924 ± 0.537
1.744SerPhe: 1.744 ± 0.372
6.788SerGly: 6.788 ± 1.076
1.557SerHis: 1.557 ± 0.322
3.176SerIle: 3.176 ± 0.489
2.18SerLys: 2.18 ± 0.406
5.231SerLeu: 5.231 ± 0.672
1.495SerMet: 1.495 ± 0.314
2.242SerAsn: 2.242 ± 0.466
2.989SerPro: 2.989 ± 0.415
1.495SerGln: 1.495 ± 0.277
3.176SerArg: 3.176 ± 0.41
3.301SerSer: 3.301 ± 0.691
3.674SerThr: 3.674 ± 0.548
3.737SerVal: 3.737 ± 0.434
1.246SerTrp: 1.246 ± 0.318
1.183SerTyr: 1.183 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
6.29ThrAla: 6.29 ± 0.816
0.311ThrCys: 0.311 ± 0.149
4.484ThrAsp: 4.484 ± 0.619
3.799ThrGlu: 3.799 ± 0.564
2.18ThrPhe: 2.18 ± 0.412
6.29ThrGly: 6.29 ± 0.6
0.996ThrHis: 0.996 ± 0.319
2.678ThrIle: 2.678 ± 0.535
2.553ThrLys: 2.553 ± 0.366
5.854ThrLeu: 5.854 ± 0.636
1.059ThrMet: 1.059 ± 0.196
1.619ThrAsn: 1.619 ± 0.382
3.861ThrPro: 3.861 ± 0.605
1.868ThrGln: 1.868 ± 0.401
3.674ThrArg: 3.674 ± 0.547
3.674ThrSer: 3.674 ± 0.589
3.924ThrThr: 3.924 ± 0.609
5.792ThrVal: 5.792 ± 0.64
1.183ThrTrp: 1.183 ± 0.286
1.806ThrTyr: 1.806 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
7.411ValAla: 7.411 ± 0.848
0.374ValCys: 0.374 ± 0.17
5.294ValAsp: 5.294 ± 0.514
5.667ValGlu: 5.667 ± 0.647
2.117ValPhe: 2.117 ± 0.345
4.795ValGly: 4.795 ± 0.693
1.308ValHis: 1.308 ± 0.256
3.363ValIle: 3.363 ± 0.479
3.488ValLys: 3.488 ± 0.413
5.356ValLeu: 5.356 ± 0.583
1.37ValMet: 1.37 ± 0.325
2.304ValAsn: 2.304 ± 0.371
4.11ValPro: 4.11 ± 0.467
2.18ValGln: 2.18 ± 0.373
4.982ValArg: 4.982 ± 0.716
4.795ValSer: 4.795 ± 0.432
5.667ValThr: 5.667 ± 0.593
4.982ValVal: 4.982 ± 0.795
1.059ValTrp: 1.059 ± 0.258
2.242ValTyr: 2.242 ± 0.37
0.0ValXaa: 0.0 ± 0.0
Trp
1.495TrpAla: 1.495 ± 0.302
0.311TrpCys: 0.311 ± 0.14
1.308TrpAsp: 1.308 ± 0.264
0.996TrpGlu: 0.996 ± 0.225
0.872TrpPhe: 0.872 ± 0.239
2.117TrpGly: 2.117 ± 0.368
0.374TrpHis: 0.374 ± 0.159
1.37TrpIle: 1.37 ± 0.27
0.374TrpLys: 0.374 ± 0.186
1.931TrpLeu: 1.931 ± 0.352
0.249TrpMet: 0.249 ± 0.113
0.498TrpAsn: 0.498 ± 0.155
0.747TrpPro: 0.747 ± 0.246
0.81TrpGln: 0.81 ± 0.218
1.183TrpArg: 1.183 ± 0.289
0.872TrpSer: 0.872 ± 0.202
1.682TrpThr: 1.682 ± 0.363
2.242TrpVal: 2.242 ± 0.286
0.561TrpTrp: 0.561 ± 0.214
0.187TrpTyr: 0.187 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.429TyrAla: 2.429 ± 0.34
0.249TyrCys: 0.249 ± 0.149
1.37TyrAsp: 1.37 ± 0.316
2.429TyrGlu: 2.429 ± 0.334
0.561TyrPhe: 0.561 ± 0.151
2.553TyrGly: 2.553 ± 0.356
0.747TyrHis: 0.747 ± 0.207
1.557TyrIle: 1.557 ± 0.336
1.246TyrLys: 1.246 ± 0.244
2.491TyrLeu: 2.491 ± 0.381
0.623TyrMet: 0.623 ± 0.165
1.246TyrAsn: 1.246 ± 0.267
1.246TyrPro: 1.246 ± 0.254
1.308TyrGln: 1.308 ± 0.318
2.616TyrArg: 2.616 ± 0.415
1.37TyrSer: 1.37 ± 0.258
1.931TyrThr: 1.931 ± 0.379
1.682TyrVal: 1.682 ± 0.354
0.436TyrTrp: 0.436 ± 0.169
0.561TyrTyr: 0.561 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16058 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski