Halovivax ruber (strain DSM 18193 / JCM 13892 / XH-70)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halovivax; Halovivax ruber

Average proteome isoelectric point is 4.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0II40|L0II40_HALRX Uroporphyrinogen-III synthase OS=Halovivax ruber (strain DSM 18193 / JCM 13892 / XH-70) OX=797302 GN=Halru_3086 PE=4 SV=1
MM1 pKa = 7.46CTRR4 pKa = 11.84DD5 pKa = 3.63SPSSGDD11 pKa = 3.28ARR13 pKa = 11.84PRR15 pKa = 11.84RR16 pKa = 11.84AVLTGAAVVGLSALAGCLDD35 pKa = 4.24GSDD38 pKa = 4.44GDD40 pKa = 5.04ADD42 pKa = 4.01APDD45 pKa = 5.86PITIEE50 pKa = 4.1PDD52 pKa = 3.35RR53 pKa = 11.84ACDD56 pKa = 3.36NCTMRR61 pKa = 11.84IGDD64 pKa = 3.73YY65 pKa = 10.78SGTAGQSFYY74 pKa = 11.18DD75 pKa = 4.0DD76 pKa = 4.17PEE78 pKa = 5.73AVLGPGDD85 pKa = 3.89DD86 pKa = 3.92TDD88 pKa = 5.63RR89 pKa = 11.84PAQFCSARR97 pKa = 11.84CTYY100 pKa = 10.48TFTFDD105 pKa = 3.47NEE107 pKa = 4.11AEE109 pKa = 4.15AEE111 pKa = 4.24PVVSYY116 pKa = 9.21LTDD119 pKa = 3.33YY120 pKa = 11.34SAVDD124 pKa = 3.24WSVDD128 pKa = 3.25TGGAAPTMSRR138 pKa = 11.84HH139 pKa = 5.92LDD141 pKa = 3.15ADD143 pKa = 3.78AFAPAADD150 pKa = 3.82LTLVVDD156 pKa = 4.41SDD158 pKa = 4.32VEE160 pKa = 4.3GAMGRR165 pKa = 11.84SVLGFSNADD174 pKa = 3.5DD175 pKa = 4.42AEE177 pKa = 4.46SFQAEE182 pKa = 4.58HH183 pKa = 6.61GGEE186 pKa = 4.29LYY188 pKa = 10.84DD189 pKa = 5.3HH190 pKa = 7.33EE191 pKa = 5.48DD192 pKa = 3.32VSPTLIQSLMGG203 pKa = 4.01

Molecular weight: 21.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0IG69|L0IG69_HALRX Putative oxidoreductase aryl-alcohol dehydrogenase like protein OS=Halovivax ruber (strain DSM 18193 / JCM 13892 / XH-70) OX=797302 GN=Halru_2644 PE=4 SV=1
MM1 pKa = 7.57TDD3 pKa = 3.01AFVPASALYY12 pKa = 10.29DD13 pKa = 3.46VEE15 pKa = 4.31TRR17 pKa = 11.84STFVHH22 pKa = 6.66PAKK25 pKa = 9.99QASEE29 pKa = 4.02IEE31 pKa = 4.34SPKK34 pKa = 9.94TSGDD38 pKa = 3.65AIEE41 pKa = 5.31ADD43 pKa = 3.88VIQAVDD49 pKa = 3.13ALGYY53 pKa = 10.81VGDD56 pKa = 4.66ATVTWHH62 pKa = 7.11DD63 pKa = 3.77AEE65 pKa = 4.64TTGLLKK71 pKa = 10.6PSTALPFYY79 pKa = 10.05GIVVVMPEE87 pKa = 3.96TPIEE91 pKa = 4.02IEE93 pKa = 3.91ACQVRR98 pKa = 11.84TSNGSRR104 pKa = 11.84STRR107 pKa = 11.84GRR109 pKa = 11.84FYY111 pKa = 10.03VKK113 pKa = 10.07RR114 pKa = 11.84RR115 pKa = 11.84TRR117 pKa = 11.84VAAQRR122 pKa = 11.84SRR124 pKa = 11.84DD125 pKa = 3.63QIRR128 pKa = 11.84GRR130 pKa = 11.84RR131 pKa = 11.84QSRR134 pKa = 11.84RR135 pKa = 11.84TGRR138 pKa = 11.84QSATHH143 pKa = 6.56RR144 pKa = 11.84VRR146 pKa = 11.84NDD148 pKa = 2.83SGPRR152 pKa = 11.84TRR154 pKa = 11.84LHH156 pKa = 5.8GNARR160 pKa = 11.84RR161 pKa = 11.84SS162 pKa = 3.37

Molecular weight: 17.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3099

0

3099

914088

29

3386

295.0

31.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.092 ± 0.056

0.747 ± 0.015

8.821 ± 0.056

8.23 ± 0.057

3.153 ± 0.026

8.523 ± 0.043

2.046 ± 0.02

4.274 ± 0.032

1.567 ± 0.025

8.72 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.02

2.131 ± 0.022

4.808 ± 0.03

2.438 ± 0.025

6.445 ± 0.044

5.858 ± 0.036

6.833 ± 0.036

8.912 ± 0.044

1.118 ± 0.019

2.602 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski