Virgibacillus phage Mimir87

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IJT7|A0A482IJT7_9CAUD Replication protein O OS=Virgibacillus phage Mimir87 OX=2530052 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 5.7EE3 pKa = 3.83IKK5 pKa = 10.39QLRR8 pKa = 11.84EE9 pKa = 3.42WMLDD13 pKa = 2.97SNYY16 pKa = 9.81PVEE19 pKa = 5.19AVALMSDD26 pKa = 3.75EE27 pKa = 4.31EE28 pKa = 4.7VVAEE32 pKa = 4.13YY33 pKa = 10.63EE34 pKa = 4.3AVTGDD39 pKa = 3.89YY40 pKa = 9.99STVSPIYY47 pKa = 10.54

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IFB4|A0A482IFB4_9CAUD Uncharacterized protein OS=Virgibacillus phage Mimir87 OX=2530052 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.47KK3 pKa = 10.32LFNRR7 pKa = 11.84SVQRR11 pKa = 11.84KK12 pKa = 5.45EE13 pKa = 4.01RR14 pKa = 11.84IMIFYY19 pKa = 9.92IDD21 pKa = 3.8SNNNVTQRR29 pKa = 11.84IIKK32 pKa = 9.25VISIEE37 pKa = 4.07EE38 pKa = 4.0SSVIAFCYY46 pKa = 8.92YY47 pKa = 9.63RR48 pKa = 11.84KK49 pKa = 9.84KK50 pKa = 10.96VRR52 pKa = 11.84TFKK55 pKa = 10.83LSNILSVGPITKK67 pKa = 10.1RR68 pKa = 11.84VGAA71 pKa = 4.46

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14519

31

1790

213.5

24.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.646 ± 0.488

0.641 ± 0.124

6.963 ± 0.312

8.093 ± 0.36

3.829 ± 0.245

6.433 ± 0.386

1.763 ± 0.123

6.509 ± 0.247

8.961 ± 0.436

8.079 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.194

5.682 ± 0.248

2.734 ± 0.205

3.891 ± 0.223

4.119 ± 0.238

6.206 ± 0.251

5.641 ± 0.229

6.006 ± 0.271

1.081 ± 0.111

3.919 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski