Amino acid dipepetide frequency for Pseudomonas pachastrellae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.189AlaAla: 13.189 ± 0.141	1.257AlaCys: 1.257 ± 0.036	6.662AlaAsp: 6.662 ± 0.083	7.652AlaGlu: 7.652 ± 0.102	3.536AlaPhe: 3.536 ± 0.056	9.136AlaGly: 9.136 ± 0.103	2.087AlaHis: 2.087 ± 0.051	4.985AlaIle: 4.985 ± 0.073	3.007AlaLys: 3.007 ± 0.061	14.118AlaLeu: 14.118 ± 0.148	2.934AlaMet: 2.934 ± 0.05	2.949AlaAsn: 2.949 ± 0.056	4.66AlaPro: 4.66 ± 0.08	5.089AlaGln: 5.089 ± 0.078	7.172AlaArg: 7.172 ± 0.087	6.184AlaSer: 6.184 ± 0.073	4.755AlaThr: 4.755 ± 0.069	7.415AlaVal: 7.415 ± 0.088	1.489AlaTrp: 1.489 ± 0.039	2.326AlaTyr: 2.326 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.08CysAla: 1.08 ± 0.031	0.175CysCys: 0.175 ± 0.012	0.589CysAsp: 0.589 ± 0.025	0.57CysGlu: 0.57 ± 0.022	0.38CysPhe: 0.38 ± 0.021	0.954CysGly: 0.954 ± 0.029	0.299CysHis: 0.299 ± 0.017	0.499CysIle: 0.499 ± 0.023	0.269CysLys: 0.269 ± 0.017	1.099CysLeu: 1.099 ± 0.031	0.211CysMet: 0.211 ± 0.011	0.288CysAsn: 0.288 ± 0.016	0.521CysPro: 0.521 ± 0.022	0.423CysGln: 0.423 ± 0.018	0.704CysArg: 0.704 ± 0.023	0.633CysSer: 0.633 ± 0.026	0.463CysThr: 0.463 ± 0.02	0.688CysVal: 0.688 ± 0.024	0.183CysTrp: 0.183 ± 0.013	0.251CysTyr: 0.251 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.377AspAla: 6.377 ± 0.071	0.585AspCys: 0.585 ± 0.024	3.207AspAsp: 3.207 ± 0.06	3.701AspGlu: 3.701 ± 0.066	2.111AspPhe: 2.111 ± 0.047	4.522AspGly: 4.522 ± 0.068	1.136AspHis: 1.136 ± 0.036	2.88AspIle: 2.88 ± 0.058	1.746AspLys: 1.746 ± 0.041	6.055AspLeu: 6.055 ± 0.072	1.446AspMet: 1.446 ± 0.032	1.79AspAsn: 1.79 ± 0.041	2.754AspPro: 2.754 ± 0.052	2.57AspGln: 2.57 ± 0.046	3.284AspArg: 3.284 ± 0.058	3.361AspSer: 3.361 ± 0.059	2.676AspThr: 2.676 ± 0.052	3.683AspVal: 3.683 ± 0.064	1.092AspTrp: 1.092 ± 0.031	1.814AspTyr: 1.814 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.73GluAla: 6.73 ± 0.09	0.469GluCys: 0.469 ± 0.021	2.803GluAsp: 2.803 ± 0.051	3.245GluGlu: 3.245 ± 0.063	1.765GluPhe: 1.765 ± 0.041	4.038GluGly: 4.038 ± 0.065	1.694GluHis: 1.694 ± 0.036	2.754GluIle: 2.754 ± 0.057	1.92GluLys: 1.92 ± 0.049	7.482GluLeu: 7.482 ± 0.081	1.418GluMet: 1.418 ± 0.034	1.551GluAsn: 1.551 ± 0.04	2.748GluPro: 2.748 ± 0.059	4.724GluGln: 4.724 ± 0.068	5.309GluArg: 5.309 ± 0.072	2.679GluSer: 2.679 ± 0.05	2.606GluThr: 2.606 ± 0.052	4.452GluVal: 4.452 ± 0.062	0.726GluTrp: 0.726 ± 0.025	1.344GluTyr: 1.344 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.84PheAla: 3.84 ± 0.063	0.463PheCys: 0.463 ± 0.019	2.436PheAsp: 2.436 ± 0.052	2.057PheGlu: 2.057 ± 0.052	1.396PhePhe: 1.396 ± 0.041	3.035PheGly: 3.035 ± 0.049	0.718PheHis: 0.718 ± 0.025	1.781PheIle: 1.781 ± 0.041	1.06PheLys: 1.06 ± 0.033	2.969PheLeu: 2.969 ± 0.046	0.874PheMet: 0.874 ± 0.03	1.315PheAsn: 1.315 ± 0.035	1.316PhePro: 1.316 ± 0.035	1.113PheGln: 1.113 ± 0.029	1.81PheArg: 1.81 ± 0.043	2.443PheSer: 2.443 ± 0.053	1.745PheThr: 1.745 ± 0.042	2.5PheVal: 2.5 ± 0.053	0.537PheTrp: 0.537 ± 0.024	1.015PheTyr: 1.015 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	7.415GlyAla: 7.415 ± 0.084	0.983GlyCys: 0.983 ± 0.032	4.176GlyAsp: 4.176 ± 0.064	4.97GlyGlu: 4.97 ± 0.073	3.164GlyPhe: 3.164 ± 0.052	5.917GlyGly: 5.917 ± 0.085	1.778GlyHis: 1.778 ± 0.042	4.036GlyIle: 4.036 ± 0.064	3.007GlyLys: 3.007 ± 0.06	8.922GlyLeu: 8.922 ± 0.099	2.359GlyMet: 2.359 ± 0.045	2.406GlyAsn: 2.406 ± 0.065	2.505GlyPro: 2.505 ± 0.046	3.636GlyGln: 3.636 ± 0.067	4.865GlyArg: 4.865 ± 0.069	4.68GlySer: 4.68 ± 0.064	3.608GlyThr: 3.608 ± 0.05	5.947GlyVal: 5.947 ± 0.075	1.276GlyTrp: 1.276 ± 0.034	2.537GlyTyr: 2.537 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.242HisAla: 2.242 ± 0.047	0.335HisCys: 0.335 ± 0.017	1.176HisAsp: 1.176 ± 0.031	1.218HisGlu: 1.218 ± 0.031	0.956HisPhe: 0.956 ± 0.029	1.824HisGly: 1.824 ± 0.043	0.647HisHis: 0.647 ± 0.028	1.06HisIle: 1.06 ± 0.028	0.565HisLys: 0.565 ± 0.023	2.458HisLeu: 2.458 ± 0.05	0.519HisMet: 0.519 ± 0.021	0.669HisAsn: 0.669 ± 0.025	1.363HisPro: 1.363 ± 0.037	1.086HisGln: 1.086 ± 0.031	1.39HisArg: 1.39 ± 0.036	1.353HisSer: 1.353 ± 0.036	0.99HisThr: 0.99 ± 0.033	1.188HisVal: 1.188 ± 0.033	0.439HisTrp: 0.439 ± 0.021	0.787HisTyr: 0.787 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.593IleAla: 5.593 ± 0.077	0.473IleCys: 0.473 ± 0.024	3.421IleAsp: 3.421 ± 0.062	3.424IleGlu: 3.424 ± 0.057	1.36IlePhe: 1.36 ± 0.036	4.159IleGly: 4.159 ± 0.079	0.993IleHis: 0.993 ± 0.029	2.088IleIle: 2.088 ± 0.048	1.6IleLys: 1.6 ± 0.043	3.97IleLeu: 3.97 ± 0.068	0.938IleMet: 0.938 ± 0.028	1.911IleAsn: 1.911 ± 0.039	2.183IlePro: 2.183 ± 0.048	1.627IleGln: 1.627 ± 0.04	2.935IleArg: 2.935 ± 0.048	3.031IleSer: 3.031 ± 0.059	2.607IleThr: 2.607 ± 0.049	2.988IleVal: 2.988 ± 0.054	0.535IleTrp: 0.535 ± 0.025	1.076IleTyr: 1.076 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.56LysAla: 3.56 ± 0.069	0.165LysCys: 0.165 ± 0.013	1.453LysAsp: 1.453 ± 0.039	1.504LysGlu: 1.504 ± 0.04	0.661LysPhe: 0.661 ± 0.022	2.307LysGly: 2.307 ± 0.053	0.662LysHis: 0.662 ± 0.024	1.293LysIle: 1.293 ± 0.043	1.099LysLys: 1.099 ± 0.045	3.284LysLeu: 3.284 ± 0.055	0.658LysMet: 0.658 ± 0.025	0.79LysAsn: 0.79 ± 0.028	1.866LysPro: 1.866 ± 0.044	1.523LysGln: 1.523 ± 0.043	2.301LysArg: 2.301 ± 0.04	1.573LysSer: 1.573 ± 0.045	1.562LysThr: 1.562 ± 0.042	2.46LysVal: 2.46 ± 0.045	0.312LysTrp: 0.312 ± 0.017	0.616LysTyr: 0.616 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.73LeuAla: 13.73 ± 0.146	1.293LeuCys: 1.293 ± 0.031	6.983LeuAsp: 6.983 ± 0.09	6.702LeuGlu: 6.702 ± 0.074	4.057LeuPhe: 4.057 ± 0.067	8.938LeuGly: 8.938 ± 0.111	2.55LeuHis: 2.55 ± 0.048	5.536LeuIle: 5.536 ± 0.089	3.685LeuLys: 3.685 ± 0.067	14.033LeuLeu: 14.033 ± 0.208	2.597LeuMet: 2.597 ± 0.048	3.522LeuAsn: 3.522 ± 0.055	6.297LeuPro: 6.297 ± 0.077	5.7LeuGln: 5.7 ± 0.098	7.795LeuArg: 7.795 ± 0.086	7.191LeuSer: 7.191 ± 0.088	5.794LeuThr: 5.794 ± 0.072	7.875LeuVal: 7.875 ± 0.092	1.426LeuTrp: 1.426 ± 0.037	2.563LeuTyr: 2.563 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.828MetAla: 2.828 ± 0.05	0.161MetCys: 0.161 ± 0.013	1.181MetAsp: 1.181 ± 0.034	1.088MetGlu: 1.088 ± 0.032	0.669MetPhe: 0.669 ± 0.025	1.807MetGly: 1.807 ± 0.039	0.569MetHis: 0.569 ± 0.02	1.067MetIle: 1.067 ± 0.029	0.835MetLys: 0.835 ± 0.027	2.9MetLeu: 2.9 ± 0.05	0.589MetMet: 0.589 ± 0.025	0.796MetAsn: 0.796 ± 0.028	1.416MetPro: 1.416 ± 0.036	1.315MetGln: 1.315 ± 0.035	1.558MetArg: 1.558 ± 0.035	1.748MetSer: 1.748 ± 0.037	1.488MetThr: 1.488 ± 0.038	1.632MetVal: 1.632 ± 0.039	0.182MetTrp: 0.182 ± 0.012	0.4MetTyr: 0.4 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.212AsnAla: 3.212 ± 0.055	0.305AsnCys: 0.305 ± 0.014	1.578AsnAsp: 1.578 ± 0.034	1.572AsnGlu: 1.572 ± 0.043	0.943AsnPhe: 0.943 ± 0.025	2.458AsnGly: 2.458 ± 0.049	0.611AsnHis: 0.611 ± 0.022	1.411AsnIle: 1.411 ± 0.035	0.854AsnLys: 0.854 ± 0.033	3.348AsnLeu: 3.348 ± 0.051	0.677AsnMet: 0.677 ± 0.027	0.947AsnAsn: 0.947 ± 0.033	1.973AsnPro: 1.973 ± 0.046	1.4AsnGln: 1.4 ± 0.034	2.027AsnArg: 2.027 ± 0.043	1.588AsnSer: 1.588 ± 0.042	1.542AsnThr: 1.542 ± 0.035	1.823AsnVal: 1.823 ± 0.044	0.475AsnTrp: 0.475 ± 0.021	0.786AsnTyr: 0.786 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	5.781ProAla: 5.781 ± 0.083	0.383ProCys: 0.383 ± 0.019	3.113ProAsp: 3.113 ± 0.059	3.767ProGlu: 3.767 ± 0.052	1.713ProPhe: 1.713 ± 0.04	3.942ProGly: 3.942 ± 0.066	0.967ProHis: 0.967 ± 0.031	1.868ProIle: 1.868 ± 0.038	1.215ProLys: 1.215 ± 0.032	5.588ProLeu: 5.588 ± 0.082	1.149ProMet: 1.149 ± 0.031	1.317ProAsn: 1.317 ± 0.032	1.904ProPro: 1.904 ± 0.05	2.181ProGln: 2.181 ± 0.047	2.514ProArg: 2.514 ± 0.044	2.433ProSer: 2.433 ± 0.048	2.08ProThr: 2.08 ± 0.041	3.819ProVal: 3.819 ± 0.059	0.731ProTrp: 0.731 ± 0.024	1.146ProTyr: 1.146 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	6.159GlnAla: 6.159 ± 0.081	0.358GlnCys: 0.358 ± 0.019	2.043GlnAsp: 2.043 ± 0.045	2.183GlnGlu: 2.183 ± 0.043	1.359GlnPhe: 1.359 ± 0.034	3.626GlnGly: 3.626 ± 0.06	1.35GlnHis: 1.35 ± 0.036	2.206GlnIle: 2.206 ± 0.041	1.036GlnLys: 1.036 ± 0.032	6.521GlnLeu: 6.521 ± 0.11	1.097GlnMet: 1.097 ± 0.029	1.05GlnAsn: 1.05 ± 0.03	2.684GlnPro: 2.684 ± 0.05	3.683GlnGln: 3.683 ± 0.081	4.195GlnArg: 4.195 ± 0.068	2.461GlnSer: 2.461 ± 0.053	2.101GlnThr: 2.101 ± 0.047	3.704GlnVal: 3.704 ± 0.064	0.722GlnTrp: 0.722 ± 0.028	1.081GlnTyr: 1.081 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	6.408ArgAla: 6.408 ± 0.085	0.645ArgCys: 0.645 ± 0.024	3.781ArgAsp: 3.781 ± 0.058	4.477ArgGlu: 4.477 ± 0.071	2.732ArgPhe: 2.732 ± 0.053	4.135ArgGly: 4.135 ± 0.059	1.63ArgHis: 1.63 ± 0.036	3.434ArgIle: 3.434 ± 0.06	2.018ArgLys: 2.018 ± 0.044	8.443ArgLeu: 8.443 ± 0.101	1.641ArgMet: 1.641 ± 0.036	1.934ArgAsn: 1.934 ± 0.042	2.798ArgPro: 2.798 ± 0.048	3.726ArgGln: 3.726 ± 0.06	4.696ArgArg: 4.696 ± 0.067	3.635ArgSer: 3.635 ± 0.061	2.735ArgThr: 2.735 ± 0.049	4.54ArgVal: 4.54 ± 0.067	1.23ArgTrp: 1.23 ± 0.036	2.201ArgTyr: 2.201 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.341SerAla: 6.341 ± 0.072	0.549SerCys: 0.549 ± 0.022	3.213SerAsp: 3.213 ± 0.053	3.465SerGlu: 3.465 ± 0.054	2.037SerPhe: 2.037 ± 0.044	5.166SerGly: 5.166 ± 0.077	1.245SerHis: 1.245 ± 0.035	2.604SerIle: 2.604 ± 0.056	1.605SerLys: 1.605 ± 0.042	6.889SerLeu: 6.889 ± 0.088	1.483SerMet: 1.483 ± 0.033	1.81SerAsn: 1.81 ± 0.045	2.571SerPro: 2.571 ± 0.052	2.531SerGln: 2.531 ± 0.049	3.675SerArg: 3.675 ± 0.054	3.52SerSer: 3.52 ± 0.06	2.724SerThr: 2.724 ± 0.056	3.952SerVal: 3.952 ± 0.065	0.935SerTrp: 0.935 ± 0.03	1.517SerTyr: 1.517 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	4.977ThrAla: 4.977 ± 0.067	0.443ThrCys: 0.443 ± 0.021	2.773ThrAsp: 2.773 ± 0.054	2.546ThrGlu: 2.546 ± 0.045	1.634ThrPhe: 1.634 ± 0.04	4.315ThrGly: 4.315 ± 0.056	0.98ThrHis: 0.98 ± 0.028	1.92ThrIle: 1.92 ± 0.048	0.99ThrLys: 0.99 ± 0.031	6.757ThrLeu: 6.757 ± 0.082	0.899ThrMet: 0.899 ± 0.031	1.185ThrAsn: 1.185 ± 0.036	2.942ThrPro: 2.942 ± 0.054	1.915ThrGln: 1.915 ± 0.045	3.062ThrArg: 3.062 ± 0.048	2.612ThrSer: 2.612 ± 0.053	2.294ThrThr: 2.294 ± 0.049	3.204ThrVal: 3.204 ± 0.056	0.628ThrTrp: 0.628 ± 0.026	1.104ThrTyr: 1.104 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	7.613ValAla: 7.613 ± 0.091	0.771ValCys: 0.771 ± 0.025	4.202ValAsp: 4.202 ± 0.057	4.486ValGlu: 4.486 ± 0.064	2.451ValPhe: 2.451 ± 0.047	5.032ValGly: 5.032 ± 0.066	1.369ValHis: 1.369 ± 0.034	3.79ValIle: 3.79 ± 0.066	2.122ValLys: 2.122 ± 0.046	7.978ValLeu: 7.978 ± 0.098	1.839ValMet: 1.839 ± 0.042	2.149ValAsn: 2.149 ± 0.042	3.125ValPro: 3.125 ± 0.056	2.697ValGln: 2.697 ± 0.046	4.332ValArg: 4.332 ± 0.07	4.373ValSer: 4.373 ± 0.066	3.623ValThr: 3.623 ± 0.057	5.379ValVal: 5.379 ± 0.086	0.825ValTrp: 0.825 ± 0.029	1.577ValTyr: 1.577 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.14TrpAla: 1.14 ± 0.033	0.16TrpCys: 0.16 ± 0.012	0.669TrpAsp: 0.669 ± 0.026	0.616TrpGlu: 0.616 ± 0.022	0.551TrpPhe: 0.551 ± 0.024	0.879TrpGly: 0.879 ± 0.032	0.405TrpHis: 0.405 ± 0.017	0.633TrpIle: 0.633 ± 0.026	0.362TrpLys: 0.362 ± 0.018	2.355TrpLeu: 2.355 ± 0.05	0.386TrpMet: 0.386 ± 0.02	0.399TrpAsn: 0.399 ± 0.018	0.682TrpPro: 0.682 ± 0.026	1.176TrpGln: 1.176 ± 0.039	1.106TrpArg: 1.106 ± 0.031	0.762TrpSer: 0.762 ± 0.026	0.589TrpThr: 0.589 ± 0.024	0.959TrpVal: 0.959 ± 0.029	0.244TrpTrp: 0.244 ± 0.017	0.336TrpTyr: 0.336 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.389TyrAla: 2.389 ± 0.049	0.305TyrCys: 0.305 ± 0.017	1.329TyrAsp: 1.329 ± 0.035	1.215TyrGlu: 1.215 ± 0.03	0.948TyrPhe: 0.948 ± 0.028	1.998TyrGly: 1.998 ± 0.044	0.576TyrHis: 0.576 ± 0.022	1.069TyrIle: 1.069 ± 0.036	0.653TyrLys: 0.653 ± 0.022	3.096TyrLeu: 3.096 ± 0.048	0.513TyrMet: 0.513 ± 0.022	0.755TyrAsn: 0.755 ± 0.025	1.303TyrPro: 1.303 ± 0.032	1.504TyrGln: 1.504 ± 0.033	2.105TyrArg: 2.105 ± 0.046	1.571TyrSer: 1.571 ± 0.04	1.201TyrThr: 1.201 ± 0.033	1.526TyrVal: 1.526 ± 0.037	0.419TyrTrp: 0.419 ± 0.02	0.705TyrTyr: 0.705 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3588 proteins (1158881 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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