Amino acid dipepetide frequency for Staphylococcus phage Sebago

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.712AlaAla: 0.712 ± 0.256
0.356AlaCys: 0.356 ± 0.141
2.278AlaAsp: 2.278 ± 0.467
3.987AlaGlu: 3.987 ± 0.549
2.848AlaPhe: 2.848 ± 0.691
3.418AlaGly: 3.418 ± 0.551
1.068AlaHis: 1.068 ± 0.271
4.414AlaIle: 4.414 ± 0.57
5.126AlaLys: 5.126 ± 0.591
4.699AlaLeu: 4.699 ± 0.799
1.78AlaMet: 1.78 ± 0.364
3.774AlaAsn: 3.774 ± 0.486
1.709AlaPro: 1.709 ± 0.319
2.706AlaGln: 2.706 ± 0.66
2.706AlaArg: 2.706 ± 0.403
4.058AlaSer: 4.058 ± 0.56
4.058AlaThr: 4.058 ± 0.546
2.919AlaVal: 2.919 ± 0.774
0.641AlaTrp: 0.641 ± 0.265
2.848AlaTyr: 2.848 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.285CysAla: 0.285 ± 0.151
0.071CysCys: 0.071 ± 0.064
0.214CysAsp: 0.214 ± 0.113
0.071CysGlu: 0.071 ± 0.068
0.356CysPhe: 0.356 ± 0.163
0.214CysGly: 0.214 ± 0.116
0.142CysHis: 0.142 ± 0.112
0.142CysIle: 0.142 ± 0.091
0.57CysLys: 0.57 ± 0.206
0.214CysLeu: 0.214 ± 0.122
0.214CysMet: 0.214 ± 0.136
0.427CysAsn: 0.427 ± 0.16
0.214CysPro: 0.214 ± 0.147
0.142CysGln: 0.142 ± 0.099
0.285CysArg: 0.285 ± 0.178
0.356CysSer: 0.356 ± 0.148
0.071CysThr: 0.071 ± 0.074
0.285CysVal: 0.285 ± 0.133
0.071CysTrp: 0.071 ± 0.065
0.356CysTyr: 0.356 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
4.058AspAla: 4.058 ± 0.623
0.285AspCys: 0.285 ± 0.139
5.696AspAsp: 5.696 ± 0.871
4.913AspGlu: 4.913 ± 0.669
2.99AspPhe: 2.99 ± 0.593
4.557AspGly: 4.557 ± 0.569
0.427AspHis: 0.427 ± 0.143
4.984AspIle: 4.984 ± 0.683
5.91AspLys: 5.91 ± 0.708
5.126AspLeu: 5.126 ± 0.653
1.709AspMet: 1.709 ± 0.364
3.702AspAsn: 3.702 ± 0.521
1.638AspPro: 1.638 ± 0.275
1.068AspGln: 1.068 ± 0.306
2.492AspArg: 2.492 ± 0.513
4.343AspSer: 4.343 ± 0.547
3.56AspThr: 3.56 ± 0.431
4.486AspVal: 4.486 ± 0.729
0.641AspTrp: 0.641 ± 0.243
4.272AspTyr: 4.272 ± 0.59
0.0AspXaa: 0.0 ± 0.0
Glu
3.418GluAla: 3.418 ± 0.562
0.57GluCys: 0.57 ± 0.216
4.201GluAsp: 4.201 ± 0.585
4.913GluGlu: 4.913 ± 0.835
3.204GluPhe: 3.204 ± 0.515
2.919GluGly: 2.919 ± 0.429
1.495GluHis: 1.495 ± 0.331
5.198GluIle: 5.198 ± 0.469
5.696GluLys: 5.696 ± 0.77
7.334GluLeu: 7.334 ± 0.768
2.421GluMet: 2.421 ± 0.52
4.699GluAsn: 4.699 ± 0.63
1.282GluPro: 1.282 ± 0.253
3.56GluGln: 3.56 ± 0.563
3.062GluArg: 3.062 ± 0.454
4.13GluSer: 4.13 ± 0.491
3.489GluThr: 3.489 ± 0.383
5.625GluVal: 5.625 ± 0.783
1.282GluTrp: 1.282 ± 0.284
4.058GluTyr: 4.058 ± 0.63
0.0GluXaa: 0.0 ± 0.0
Phe
2.065PheAla: 2.065 ± 0.346
0.214PheCys: 0.214 ± 0.133
4.414PheAsp: 4.414 ± 0.456
3.062PheGlu: 3.062 ± 0.543
1.353PhePhe: 1.353 ± 0.379
2.777PheGly: 2.777 ± 0.661
0.641PheHis: 0.641 ± 0.179
3.987PheIle: 3.987 ± 0.533
5.055PheLys: 5.055 ± 0.625
2.777PheLeu: 2.777 ± 0.428
1.068PheMet: 1.068 ± 0.31
3.346PheAsn: 3.346 ± 0.446
0.712PhePro: 0.712 ± 0.255
1.068PheGln: 1.068 ± 0.353
0.997PheArg: 0.997 ± 0.205
2.563PheSer: 2.563 ± 0.457
2.919PheThr: 2.919 ± 0.533
1.78PheVal: 1.78 ± 0.332
0.712PheTrp: 0.712 ± 0.264
2.278PheTyr: 2.278 ± 0.409
0.0PheXaa: 0.0 ± 0.0
Gly
4.058GlyAla: 4.058 ± 0.6
0.285GlyCys: 0.285 ± 0.125
4.272GlyAsp: 4.272 ± 0.568
3.062GlyGlu: 3.062 ± 0.529
2.848GlyPhe: 2.848 ± 0.429
2.777GlyGly: 2.777 ± 0.417
1.709GlyHis: 1.709 ± 0.375
4.557GlyIle: 4.557 ± 0.611
4.699GlyLys: 4.699 ± 0.507
4.414GlyLeu: 4.414 ± 0.618
1.709GlyMet: 1.709 ± 0.429
3.916GlyAsn: 3.916 ± 0.598
0.356GlyPro: 0.356 ± 0.152
2.492GlyGln: 2.492 ± 0.464
2.35GlyArg: 2.35 ± 0.389
2.421GlySer: 2.421 ± 0.485
4.058GlyThr: 4.058 ± 0.481
5.126GlyVal: 5.126 ± 0.628
0.997GlyTrp: 0.997 ± 0.364
3.275GlyTyr: 3.275 ± 0.53
0.0GlyXaa: 0.0 ± 0.0
His
1.139HisAla: 1.139 ± 0.313
0.0HisCys: 0.0 ± 0.0
0.641HisAsp: 0.641 ± 0.209
0.926HisGlu: 0.926 ± 0.253
0.641HisPhe: 0.641 ± 0.184
1.21HisGly: 1.21 ± 0.268
0.356HisHis: 0.356 ± 0.174
1.068HisIle: 1.068 ± 0.28
1.139HisLys: 1.139 ± 0.217
1.353HisLeu: 1.353 ± 0.334
0.214HisMet: 0.214 ± 0.107
0.854HisAsn: 0.854 ± 0.299
0.997HisPro: 0.997 ± 0.269
0.926HisGln: 0.926 ± 0.277
0.57HisArg: 0.57 ± 0.203
0.997HisSer: 0.997 ± 0.265
1.709HisThr: 1.709 ± 0.344
1.353HisVal: 1.353 ± 0.411
0.142HisTrp: 0.142 ± 0.096
0.641HisTyr: 0.641 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
4.272IleAla: 4.272 ± 0.714
0.142IleCys: 0.142 ± 0.101
6.194IleAsp: 6.194 ± 0.708
7.618IleGlu: 7.618 ± 0.806
2.919IlePhe: 2.919 ± 0.444
5.482IleGly: 5.482 ± 0.629
1.068IleHis: 1.068 ± 0.256
3.987IleIle: 3.987 ± 0.706
7.832IleLys: 7.832 ± 0.676
3.774IleLeu: 3.774 ± 0.559
2.492IleMet: 2.492 ± 0.469
4.343IleAsn: 4.343 ± 0.63
2.207IlePro: 2.207 ± 0.294
2.278IleGln: 2.278 ± 0.481
2.919IleArg: 2.919 ± 0.431
3.702IleSer: 3.702 ± 0.579
5.269IleThr: 5.269 ± 0.568
3.987IleVal: 3.987 ± 0.424
0.57IleTrp: 0.57 ± 0.27
2.421IleTyr: 2.421 ± 0.547
0.0IleXaa: 0.0 ± 0.0
Lys
5.91LysAla: 5.91 ± 0.622
0.57LysCys: 0.57 ± 0.188
5.554LysAsp: 5.554 ± 0.567
8.33LysGlu: 8.33 ± 0.894
3.631LysPhe: 3.631 ± 0.438
5.198LysGly: 5.198 ± 0.728
1.495LysHis: 1.495 ± 0.319
5.696LysIle: 5.696 ± 0.684
7.903LysLys: 7.903 ± 1.02
7.049LysLeu: 7.049 ± 0.656
2.919LysMet: 2.919 ± 0.524
5.198LysAsn: 5.198 ± 0.636
2.136LysPro: 2.136 ± 0.441
4.058LysGln: 4.058 ± 0.562
4.557LysArg: 4.557 ± 0.569
5.269LysSer: 5.269 ± 0.718
4.913LysThr: 4.913 ± 0.745
4.984LysVal: 4.984 ± 0.558
1.139LysTrp: 1.139 ± 0.287
4.058LysTyr: 4.058 ± 0.626
0.0LysXaa: 0.0 ± 0.0
Leu
4.628LeuAla: 4.628 ± 0.769
0.285LeuCys: 0.285 ± 0.148
5.198LeuAsp: 5.198 ± 0.635
5.554LeuGlu: 5.554 ± 0.652
3.204LeuPhe: 3.204 ± 0.416
3.346LeuGly: 3.346 ± 0.551
1.495LeuHis: 1.495 ± 0.303
5.126LeuIle: 5.126 ± 0.6
7.974LeuLys: 7.974 ± 0.683
6.55LeuLeu: 6.55 ± 0.822
1.922LeuMet: 1.922 ± 0.401
4.557LeuAsn: 4.557 ± 0.569
2.706LeuPro: 2.706 ± 0.401
2.99LeuGln: 2.99 ± 0.52
3.346LeuArg: 3.346 ± 0.571
4.628LeuSer: 4.628 ± 0.585
5.055LeuThr: 5.055 ± 0.616
3.631LeuVal: 3.631 ± 0.499
0.641LeuTrp: 0.641 ± 0.291
3.774LeuTyr: 3.774 ± 0.586
0.0LeuXaa: 0.0 ± 0.0
Met
2.065MetAla: 2.065 ± 0.484
0.142MetCys: 0.142 ± 0.09
1.068MetAsp: 1.068 ± 0.32
1.353MetGlu: 1.353 ± 0.291
0.997MetPhe: 0.997 ± 0.228
1.139MetGly: 1.139 ± 0.308
0.356MetHis: 0.356 ± 0.17
1.638MetIle: 1.638 ± 0.286
2.207MetLys: 2.207 ± 0.408
2.136MetLeu: 2.136 ± 0.356
0.712MetMet: 0.712 ± 0.233
1.78MetAsn: 1.78 ± 0.383
1.068MetPro: 1.068 ± 0.287
1.353MetGln: 1.353 ± 0.373
1.21MetArg: 1.21 ± 0.235
1.709MetSer: 1.709 ± 0.371
2.706MetThr: 2.706 ± 0.427
0.997MetVal: 0.997 ± 0.261
0.498MetTrp: 0.498 ± 0.166
0.712MetTyr: 0.712 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
3.631AsnAla: 3.631 ± 0.535
0.214AsnCys: 0.214 ± 0.115
4.13AsnAsp: 4.13 ± 0.476
4.842AsnGlu: 4.842 ± 0.495
3.275AsnPhe: 3.275 ± 0.498
4.984AsnGly: 4.984 ± 0.566
0.712AsnHis: 0.712 ± 0.193
4.272AsnIle: 4.272 ± 0.587
5.838AsnLys: 5.838 ± 0.704
4.13AsnLeu: 4.13 ± 0.614
0.997AsnMet: 0.997 ± 0.279
4.628AsnAsn: 4.628 ± 0.628
2.777AsnPro: 2.777 ± 0.513
2.278AsnGln: 2.278 ± 0.413
1.851AsnArg: 1.851 ± 0.329
3.631AsnSer: 3.631 ± 0.581
3.987AsnThr: 3.987 ± 0.68
4.486AsnVal: 4.486 ± 0.644
0.926AsnTrp: 0.926 ± 0.232
2.848AsnTyr: 2.848 ± 0.557
0.0AsnXaa: 0.0 ± 0.0
Pro
1.068ProAla: 1.068 ± 0.241
0.071ProCys: 0.071 ± 0.06
1.495ProAsp: 1.495 ± 0.268
1.78ProGlu: 1.78 ± 0.415
1.139ProPhe: 1.139 ± 0.249
1.638ProGly: 1.638 ± 0.389
0.427ProHis: 0.427 ± 0.197
2.563ProIle: 2.563 ± 0.331
2.99ProLys: 2.99 ± 0.509
1.78ProLeu: 1.78 ± 0.439
0.712ProMet: 0.712 ± 0.194
1.922ProAsn: 1.922 ± 0.441
0.57ProPro: 0.57 ± 0.192
0.783ProGln: 0.783 ± 0.206
1.068ProArg: 1.068 ± 0.309
1.851ProSer: 1.851 ± 0.414
2.35ProThr: 2.35 ± 0.329
1.638ProVal: 1.638 ± 0.351
0.071ProTrp: 0.071 ± 0.065
1.709ProTyr: 1.709 ± 0.384
0.0ProXaa: 0.0 ± 0.0
Gln
3.702GlnAla: 3.702 ± 0.576
0.356GlnCys: 0.356 ± 0.171
2.065GlnAsp: 2.065 ± 0.393
2.777GlnGlu: 2.777 ± 0.413
2.278GlnPhe: 2.278 ± 0.407
2.065GlnGly: 2.065 ± 0.358
0.712GlnHis: 0.712 ± 0.232
2.706GlnIle: 2.706 ± 0.438
2.634GlnLys: 2.634 ± 0.524
3.062GlnLeu: 3.062 ± 0.498
1.068GlnMet: 1.068 ± 0.259
2.563GlnAsn: 2.563 ± 0.472
1.353GlnPro: 1.353 ± 0.434
1.922GlnGln: 1.922 ± 0.502
2.207GlnArg: 2.207 ± 0.42
2.207GlnSer: 2.207 ± 0.368
1.78GlnThr: 1.78 ± 0.306
2.563GlnVal: 2.563 ± 0.404
0.285GlnTrp: 0.285 ± 0.129
1.709GlnTyr: 1.709 ± 0.371
0.0GlnXaa: 0.0 ± 0.0
Arg
1.638ArgAla: 1.638 ± 0.273
0.427ArgCys: 0.427 ± 0.18
3.275ArgAsp: 3.275 ± 0.543
2.634ArgGlu: 2.634 ± 0.441
2.35ArgPhe: 2.35 ± 0.422
2.278ArgGly: 2.278 ± 0.391
1.139ArgHis: 1.139 ± 0.247
3.062ArgIle: 3.062 ± 0.562
2.919ArgLys: 2.919 ± 0.508
3.774ArgLeu: 3.774 ± 0.564
0.854ArgMet: 0.854 ± 0.214
3.275ArgAsn: 3.275 ± 0.423
1.139ArgPro: 1.139 ± 0.27
1.922ArgGln: 1.922 ± 0.394
1.709ArgArg: 1.709 ± 0.39
1.495ArgSer: 1.495 ± 0.347
1.78ArgThr: 1.78 ± 0.382
2.136ArgVal: 2.136 ± 0.329
0.285ArgTrp: 0.285 ± 0.141
2.563ArgTyr: 2.563 ± 0.469
0.0ArgXaa: 0.0 ± 0.0
Ser
3.702SerAla: 3.702 ± 0.613
0.142SerCys: 0.142 ± 0.148
4.699SerAsp: 4.699 ± 0.668
3.702SerGlu: 3.702 ± 0.561
2.706SerPhe: 2.706 ± 0.446
3.774SerGly: 3.774 ± 0.47
0.641SerHis: 0.641 ± 0.197
5.126SerIle: 5.126 ± 0.665
6.266SerLys: 6.266 ± 0.795
3.987SerLeu: 3.987 ± 0.459
1.424SerMet: 1.424 ± 0.276
3.845SerAsn: 3.845 ± 0.598
0.997SerPro: 0.997 ± 0.259
3.133SerGln: 3.133 ± 0.456
2.136SerArg: 2.136 ± 0.331
3.418SerSer: 3.418 ± 0.465
3.489SerThr: 3.489 ± 0.54
3.987SerVal: 3.987 ± 0.685
0.854SerTrp: 0.854 ± 0.287
1.851SerTyr: 1.851 ± 0.317
0.0SerXaa: 0.0 ± 0.0
Thr
3.346ThrAla: 3.346 ± 0.452
0.071ThrCys: 0.071 ± 0.071
3.631ThrAsp: 3.631 ± 0.471
3.845ThrGlu: 3.845 ± 0.483
2.919ThrPhe: 2.919 ± 0.475
4.77ThrGly: 4.77 ± 0.689
1.282ThrHis: 1.282 ± 0.323
5.198ThrIle: 5.198 ± 0.747
4.699ThrLys: 4.699 ± 0.643
4.486ThrLeu: 4.486 ± 0.612
1.21ThrMet: 1.21 ± 0.291
4.272ThrAsn: 4.272 ± 0.723
1.851ThrPro: 1.851 ± 0.374
2.848ThrGln: 2.848 ± 0.38
2.634ThrArg: 2.634 ± 0.469
4.913ThrSer: 4.913 ± 0.818
3.845ThrThr: 3.845 ± 0.753
3.204ThrVal: 3.204 ± 0.523
0.783ThrTrp: 0.783 ± 0.295
2.278ThrTyr: 2.278 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
3.56ValAla: 3.56 ± 0.656
0.142ValCys: 0.142 ± 0.099
4.842ValAsp: 4.842 ± 0.603
4.272ValGlu: 4.272 ± 0.579
1.709ValPhe: 1.709 ± 0.391
3.204ValGly: 3.204 ± 0.567
0.641ValHis: 0.641 ± 0.232
5.269ValIle: 5.269 ± 0.6
5.838ValLys: 5.838 ± 0.6
5.34ValLeu: 5.34 ± 0.638
1.566ValMet: 1.566 ± 0.346
3.275ValAsn: 3.275 ± 0.492
2.563ValPro: 2.563 ± 0.447
1.851ValGln: 1.851 ± 0.397
2.35ValArg: 2.35 ± 0.346
4.343ValSer: 4.343 ± 0.775
3.774ValThr: 3.774 ± 0.552
3.204ValVal: 3.204 ± 0.449
0.783ValTrp: 0.783 ± 0.271
1.994ValTyr: 1.994 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
0.854TrpAla: 0.854 ± 0.26
0.071TrpCys: 0.071 ± 0.065
0.427TrpAsp: 0.427 ± 0.165
0.854TrpGlu: 0.854 ± 0.23
0.57TrpPhe: 0.57 ± 0.173
0.712TrpGly: 0.712 ± 0.302
0.427TrpHis: 0.427 ± 0.192
0.712TrpIle: 0.712 ± 0.254
0.854TrpLys: 0.854 ± 0.257
1.21TrpLeu: 1.21 ± 0.309
0.071TrpMet: 0.071 ± 0.113
0.783TrpAsn: 0.783 ± 0.242
0.071TrpPro: 0.071 ± 0.068
0.641TrpGln: 0.641 ± 0.255
0.498TrpArg: 0.498 ± 0.164
0.641TrpSer: 0.641 ± 0.249
0.712TrpThr: 0.712 ± 0.193
1.21TrpVal: 1.21 ± 0.27
0.0TrpTrp: 0.0 ± 0.0
0.641TrpTyr: 0.641 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.994TyrAla: 1.994 ± 0.358
0.285TyrCys: 0.285 ± 0.133
2.563TyrAsp: 2.563 ± 0.521
4.058TyrGlu: 4.058 ± 0.581
1.851TyrPhe: 1.851 ± 0.39
2.919TyrGly: 2.919 ± 0.602
0.641TyrHis: 0.641 ± 0.226
3.774TyrIle: 3.774 ± 0.574
4.414TyrLys: 4.414 ± 0.564
3.418TyrLeu: 3.418 ± 0.566
0.712TyrMet: 0.712 ± 0.227
3.275TyrAsn: 3.275 ± 0.477
1.282TyrPro: 1.282 ± 0.349
1.994TyrGln: 1.994 ± 0.359
1.709TyrArg: 1.709 ± 0.51
3.204TyrSer: 3.204 ± 0.457
2.563TyrThr: 2.563 ± 0.423
2.919TyrVal: 2.919 ± 0.426
0.712TyrTrp: 0.712 ± 0.2
1.78TyrTyr: 1.78 ± 0.334
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (14046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski