Amino acid dipepetide frequency for Mycobacterium phage Sagefire

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.394AlaAla: 13.394 ± 1.461
0.806AlaCys: 0.806 ± 0.221
6.697AlaAsp: 6.697 ± 0.6
6.635AlaGlu: 6.635 ± 0.806
3.348AlaPhe: 3.348 ± 0.518
7.627AlaGly: 7.627 ± 0.667
1.364AlaHis: 1.364 ± 0.329
4.279AlaIle: 4.279 ± 0.557
3.906AlaLys: 3.906 ± 0.527
9.549AlaLeu: 9.549 ± 0.777
2.542AlaMet: 2.542 ± 0.435
2.48AlaAsn: 2.48 ± 0.363
5.209AlaPro: 5.209 ± 0.737
2.852AlaGln: 2.852 ± 0.54
6.263AlaArg: 6.263 ± 0.597
5.395AlaSer: 5.395 ± 0.644
5.519AlaThr: 5.519 ± 0.704
8.681AlaVal: 8.681 ± 0.959
1.984AlaTrp: 1.984 ± 0.323
2.914AlaTyr: 2.914 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.558CysAla: 0.558 ± 0.164
0.062CysCys: 0.062 ± 0.064
0.434CysAsp: 0.434 ± 0.154
0.744CysGlu: 0.744 ± 0.206
0.186CysPhe: 0.186 ± 0.103
0.372CysGly: 0.372 ± 0.159
0.186CysHis: 0.186 ± 0.115
0.248CysIle: 0.248 ± 0.129
0.186CysLys: 0.186 ± 0.11
0.496CysLeu: 0.496 ± 0.221
0.124CysMet: 0.124 ± 0.087
0.31CysAsn: 0.31 ± 0.129
0.372CysPro: 0.372 ± 0.156
0.372CysGln: 0.372 ± 0.147
0.744CysArg: 0.744 ± 0.226
0.248CysSer: 0.248 ± 0.12
0.372CysThr: 0.372 ± 0.194
0.31CysVal: 0.31 ± 0.15
0.248CysTrp: 0.248 ± 0.123
0.124CysTyr: 0.124 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
6.573AspAla: 6.573 ± 0.746
0.496AspCys: 0.496 ± 0.172
4.713AspAsp: 4.713 ± 0.53
3.906AspGlu: 3.906 ± 0.452
2.356AspPhe: 2.356 ± 0.377
6.449AspGly: 6.449 ± 0.647
1.116AspHis: 1.116 ± 0.258
2.666AspIle: 2.666 ± 0.38
2.604AspLys: 2.604 ± 0.425
6.511AspLeu: 6.511 ± 0.709
0.868AspMet: 0.868 ± 0.181
1.984AspAsn: 1.984 ± 0.34
5.023AspPro: 5.023 ± 0.621
1.736AspGln: 1.736 ± 0.412
3.72AspArg: 3.72 ± 0.47
2.976AspSer: 2.976 ± 0.422
3.782AspThr: 3.782 ± 0.398
4.217AspVal: 4.217 ± 0.49
1.86AspTrp: 1.86 ± 0.349
2.046AspTyr: 2.046 ± 0.344
0.0AspXaa: 0.0 ± 0.0
Glu
5.953GluAla: 5.953 ± 0.633
0.248GluCys: 0.248 ± 0.154
4.899GluAsp: 4.899 ± 0.681
4.961GluGlu: 4.961 ± 0.57
1.86GluPhe: 1.86 ± 0.298
3.782GluGly: 3.782 ± 0.428
1.612GluHis: 1.612 ± 0.365
3.472GluIle: 3.472 ± 0.416
2.728GluLys: 2.728 ± 0.403
7.007GluLeu: 7.007 ± 0.609
1.736GluMet: 1.736 ± 0.334
1.798GluAsn: 1.798 ± 0.334
2.728GluPro: 2.728 ± 0.504
2.232GluGln: 2.232 ± 0.346
3.969GluArg: 3.969 ± 0.543
3.72GluSer: 3.72 ± 0.453
3.658GluThr: 3.658 ± 0.434
5.395GluVal: 5.395 ± 0.544
1.364GluTrp: 1.364 ± 0.314
2.232GluTyr: 2.232 ± 0.426
0.0GluXaa: 0.0 ± 0.0
Phe
2.356PheAla: 2.356 ± 0.316
0.372PheCys: 0.372 ± 0.173
2.79PheAsp: 2.79 ± 0.36
2.108PheGlu: 2.108 ± 0.39
0.558PhePhe: 0.558 ± 0.145
3.472PheGly: 3.472 ± 0.44
0.744PheHis: 0.744 ± 0.207
1.302PheIle: 1.302 ± 0.301
1.24PheLys: 1.24 ± 0.254
2.17PheLeu: 2.17 ± 0.401
0.62PheMet: 0.62 ± 0.196
1.054PheAsn: 1.054 ± 0.24
1.55PhePro: 1.55 ± 0.281
1.116PheGln: 1.116 ± 0.266
1.86PheArg: 1.86 ± 0.365
1.798PheSer: 1.798 ± 0.441
2.108PheThr: 2.108 ± 0.405
2.046PheVal: 2.046 ± 0.374
0.62PheTrp: 0.62 ± 0.161
0.744PheTyr: 0.744 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
7.937GlyAla: 7.937 ± 0.987
0.806GlyCys: 0.806 ± 0.284
5.705GlyAsp: 5.705 ± 0.542
4.837GlyGlu: 4.837 ± 0.468
2.852GlyPhe: 2.852 ± 0.504
8.123GlyGly: 8.123 ± 1.514
1.736GlyHis: 1.736 ± 0.318
4.403GlyIle: 4.403 ± 0.693
3.41GlyLys: 3.41 ± 0.508
7.441GlyLeu: 7.441 ± 0.897
1.922GlyMet: 1.922 ± 0.318
3.038GlyAsn: 3.038 ± 0.368
3.844GlyPro: 3.844 ± 0.558
1.922GlyGln: 1.922 ± 0.331
5.209GlyArg: 5.209 ± 0.544
6.449GlySer: 6.449 ± 0.705
5.147GlyThr: 5.147 ± 0.633
5.147GlyVal: 5.147 ± 0.609
2.542GlyTrp: 2.542 ± 0.367
2.976GlyTyr: 2.976 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
1.674HisAla: 1.674 ± 0.337
0.186HisCys: 0.186 ± 0.137
0.992HisAsp: 0.992 ± 0.205
1.736HisGlu: 1.736 ± 0.398
0.744HisPhe: 0.744 ± 0.205
1.488HisGly: 1.488 ± 0.334
0.744HisHis: 0.744 ± 0.229
1.116HisIle: 1.116 ± 0.222
0.992HisLys: 0.992 ± 0.291
1.488HisLeu: 1.488 ± 0.355
0.186HisMet: 0.186 ± 0.146
0.248HisAsn: 0.248 ± 0.115
1.24HisPro: 1.24 ± 0.248
0.992HisGln: 0.992 ± 0.211
1.302HisArg: 1.302 ± 0.256
0.806HisSer: 0.806 ± 0.183
0.992HisThr: 0.992 ± 0.241
1.426HisVal: 1.426 ± 0.287
0.62HisTrp: 0.62 ± 0.178
0.496HisTyr: 0.496 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
6.573IleAla: 6.573 ± 0.753
0.372IleCys: 0.372 ± 0.172
3.224IleAsp: 3.224 ± 0.391
3.348IleGlu: 3.348 ± 0.449
0.868IlePhe: 0.868 ± 0.238
3.906IleGly: 3.906 ± 0.504
1.054IleHis: 1.054 ± 0.294
1.488IleIle: 1.488 ± 0.289
1.426IleLys: 1.426 ± 0.298
3.782IleLeu: 3.782 ± 0.447
0.682IleMet: 0.682 ± 0.178
1.736IleAsn: 1.736 ± 0.295
3.162IlePro: 3.162 ± 0.394
1.612IleGln: 1.612 ± 0.358
3.72IleArg: 3.72 ± 0.504
3.534IleSer: 3.534 ± 0.44
3.1IleThr: 3.1 ± 0.489
2.976IleVal: 2.976 ± 0.468
0.744IleTrp: 0.744 ± 0.198
1.55IleTyr: 1.55 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
3.844LysAla: 3.844 ± 0.478
0.186LysCys: 0.186 ± 0.108
2.356LysAsp: 2.356 ± 0.367
2.17LysGlu: 2.17 ± 0.379
1.612LysPhe: 1.612 ± 0.314
2.48LysGly: 2.48 ± 0.43
1.116LysHis: 1.116 ± 0.297
2.542LysIle: 2.542 ± 0.444
1.612LysLys: 1.612 ± 0.376
3.534LysLeu: 3.534 ± 0.448
0.868LysMet: 0.868 ± 0.198
1.426LysAsn: 1.426 ± 0.318
2.914LysPro: 2.914 ± 0.421
1.488LysGln: 1.488 ± 0.282
2.914LysArg: 2.914 ± 0.411
2.108LysSer: 2.108 ± 0.363
2.294LysThr: 2.294 ± 0.393
2.914LysVal: 2.914 ± 0.457
0.868LysTrp: 0.868 ± 0.225
1.178LysTyr: 1.178 ± 0.311
0.0LysXaa: 0.0 ± 0.0
Leu
9.859LeuAla: 9.859 ± 0.851
0.31LeuCys: 0.31 ± 0.139
6.015LeuAsp: 6.015 ± 0.591
5.581LeuGlu: 5.581 ± 0.536
1.86LeuPhe: 1.86 ± 0.403
7.565LeuGly: 7.565 ± 0.866
1.488LeuHis: 1.488 ± 0.326
4.589LeuIle: 4.589 ± 0.553
3.969LeuLys: 3.969 ± 0.546
5.829LeuLeu: 5.829 ± 0.494
1.86LeuMet: 1.86 ± 0.284
3.1LeuAsn: 3.1 ± 0.377
5.643LeuPro: 5.643 ± 0.476
2.542LeuGln: 2.542 ± 0.41
6.077LeuArg: 6.077 ± 0.555
5.705LeuSer: 5.705 ± 0.612
6.325LeuThr: 6.325 ± 0.518
5.085LeuVal: 5.085 ± 0.562
1.178LeuTrp: 1.178 ± 0.322
2.294LeuTyr: 2.294 ± 0.421
0.0LeuXaa: 0.0 ± 0.0
Met
2.232MetAla: 2.232 ± 0.281
0.062MetCys: 0.062 ± 0.058
1.364MetAsp: 1.364 ± 0.244
1.302MetGlu: 1.302 ± 0.302
0.62MetPhe: 0.62 ± 0.192
1.426MetGly: 1.426 ± 0.281
0.558MetHis: 0.558 ± 0.261
0.62MetIle: 0.62 ± 0.182
1.054MetLys: 1.054 ± 0.267
1.116MetLeu: 1.116 ± 0.248
0.186MetMet: 0.186 ± 0.104
0.992MetAsn: 0.992 ± 0.247
1.054MetPro: 1.054 ± 0.291
0.558MetGln: 0.558 ± 0.18
1.24MetArg: 1.24 ± 0.301
2.418MetSer: 2.418 ± 0.428
1.922MetThr: 1.922 ± 0.296
1.178MetVal: 1.178 ± 0.285
0.31MetTrp: 0.31 ± 0.131
0.372MetTyr: 0.372 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
2.728AsnAla: 2.728 ± 0.518
0.124AsnCys: 0.124 ± 0.088
2.046AsnAsp: 2.046 ± 0.358
1.736AsnGlu: 1.736 ± 0.315
0.868AsnPhe: 0.868 ± 0.229
3.596AsnGly: 3.596 ± 0.535
0.682AsnHis: 0.682 ± 0.225
1.488AsnIle: 1.488 ± 0.269
0.682AsnLys: 0.682 ± 0.202
2.48AsnLeu: 2.48 ± 0.345
0.744AsnMet: 0.744 ± 0.183
0.744AsnAsn: 0.744 ± 0.163
2.79AsnPro: 2.79 ± 0.356
1.054AsnGln: 1.054 ± 0.232
1.488AsnArg: 1.488 ± 0.306
1.798AsnSer: 1.798 ± 0.367
1.984AsnThr: 1.984 ± 0.365
2.604AsnVal: 2.604 ± 0.421
0.744AsnTrp: 0.744 ± 0.201
1.116AsnTyr: 1.116 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
5.519ProAla: 5.519 ± 0.623
0.31ProCys: 0.31 ± 0.151
4.589ProAsp: 4.589 ± 0.526
4.155ProGlu: 4.155 ± 0.522
1.984ProPhe: 1.984 ± 0.36
5.023ProGly: 5.023 ± 0.584
0.806ProHis: 0.806 ± 0.202
2.48ProIle: 2.48 ± 0.401
2.232ProLys: 2.232 ± 0.328
4.465ProLeu: 4.465 ± 0.577
0.806ProMet: 0.806 ± 0.263
1.488ProAsn: 1.488 ± 0.319
2.79ProPro: 2.79 ± 0.393
1.612ProGln: 1.612 ± 0.353
2.666ProArg: 2.666 ± 0.511
3.906ProSer: 3.906 ± 0.466
4.837ProThr: 4.837 ± 0.558
4.031ProVal: 4.031 ± 0.506
0.93ProTrp: 0.93 ± 0.294
1.612ProTyr: 1.612 ± 0.34
0.0ProXaa: 0.0 ± 0.0
Gln
3.162GlnAla: 3.162 ± 0.463
0.124GlnCys: 0.124 ± 0.091
1.116GlnAsp: 1.116 ± 0.333
1.55GlnGlu: 1.55 ± 0.299
1.116GlnPhe: 1.116 ± 0.275
2.418GlnGly: 2.418 ± 0.339
0.434GlnHis: 0.434 ± 0.14
2.852GlnIle: 2.852 ± 0.523
1.178GlnLys: 1.178 ± 0.284
4.031GlnLeu: 4.031 ± 0.515
0.744GlnMet: 0.744 ± 0.24
0.682GlnAsn: 0.682 ± 0.207
1.674GlnPro: 1.674 ± 0.336
1.86GlnGln: 1.86 ± 0.387
1.984GlnArg: 1.984 ± 0.364
1.488GlnSer: 1.488 ± 0.29
1.798GlnThr: 1.798 ± 0.262
2.728GlnVal: 2.728 ± 0.401
0.682GlnTrp: 0.682 ± 0.186
0.496GlnTyr: 0.496 ± 0.141
0.0GlnXaa: 0.0 ± 0.0
Arg
5.333ArgAla: 5.333 ± 0.708
0.744ArgCys: 0.744 ± 0.239
3.1ArgAsp: 3.1 ± 0.507
4.527ArgGlu: 4.527 ± 0.595
1.984ArgPhe: 1.984 ± 0.364
5.767ArgGly: 5.767 ± 0.781
1.116ArgHis: 1.116 ± 0.22
3.1ArgIle: 3.1 ± 0.46
3.41ArgLys: 3.41 ± 0.546
5.767ArgLeu: 5.767 ± 0.658
1.86ArgMet: 1.86 ± 0.351
2.294ArgAsn: 2.294 ± 0.469
2.604ArgPro: 2.604 ± 0.366
1.736ArgGln: 1.736 ± 0.343
5.395ArgArg: 5.395 ± 0.745
3.348ArgSer: 3.348 ± 0.505
3.286ArgThr: 3.286 ± 0.479
5.209ArgVal: 5.209 ± 0.557
1.488ArgTrp: 1.488 ± 0.387
1.488ArgTyr: 1.488 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
6.201SerAla: 6.201 ± 0.855
0.496SerCys: 0.496 ± 0.181
3.472SerAsp: 3.472 ± 0.443
3.472SerGlu: 3.472 ± 0.547
2.108SerPhe: 2.108 ± 0.337
6.635SerGly: 6.635 ± 0.72
1.116SerHis: 1.116 ± 0.272
2.914SerIle: 2.914 ± 0.444
2.232SerLys: 2.232 ± 0.382
5.147SerLeu: 5.147 ± 0.594
1.364SerMet: 1.364 ± 0.251
2.294SerAsn: 2.294 ± 0.399
3.038SerPro: 3.038 ± 0.448
1.922SerGln: 1.922 ± 0.327
3.038SerArg: 3.038 ± 0.354
3.286SerSer: 3.286 ± 0.674
3.906SerThr: 3.906 ± 0.508
3.72SerVal: 3.72 ± 0.392
1.426SerTrp: 1.426 ± 0.341
1.426SerTyr: 1.426 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
6.139ThrAla: 6.139 ± 0.757
0.31ThrCys: 0.31 ± 0.172
4.155ThrAsp: 4.155 ± 0.533
4.217ThrGlu: 4.217 ± 0.525
2.232ThrPhe: 2.232 ± 0.432
6.759ThrGly: 6.759 ± 0.604
1.054ThrHis: 1.054 ± 0.274
3.038ThrIle: 3.038 ± 0.514
2.542ThrLys: 2.542 ± 0.35
6.201ThrLeu: 6.201 ± 0.678
1.054ThrMet: 1.054 ± 0.208
1.798ThrAsn: 1.798 ± 0.343
4.217ThrPro: 4.217 ± 0.564
1.984ThrGln: 1.984 ± 0.412
3.41ThrArg: 3.41 ± 0.574
3.348ThrSer: 3.348 ± 0.547
4.465ThrThr: 4.465 ± 0.553
5.085ThrVal: 5.085 ± 0.568
1.178ThrTrp: 1.178 ± 0.255
1.86ThrTyr: 1.86 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
6.945ValAla: 6.945 ± 0.712
0.31ValCys: 0.31 ± 0.116
5.457ValAsp: 5.457 ± 0.516
5.023ValGlu: 5.023 ± 0.578
2.046ValPhe: 2.046 ± 0.298
4.403ValGly: 4.403 ± 0.664
1.302ValHis: 1.302 ± 0.256
3.72ValIle: 3.72 ± 0.485
3.038ValLys: 3.038 ± 0.44
5.147ValLeu: 5.147 ± 0.534
1.302ValMet: 1.302 ± 0.319
2.48ValAsn: 2.48 ± 0.352
4.217ValPro: 4.217 ± 0.458
2.604ValGln: 2.604 ± 0.432
4.961ValArg: 4.961 ± 0.714
4.403ValSer: 4.403 ± 0.487
5.767ValThr: 5.767 ± 0.596
5.705ValVal: 5.705 ± 0.739
1.364ValTrp: 1.364 ± 0.326
1.922ValTyr: 1.922 ± 0.352
0.0ValXaa: 0.0 ± 0.0
Trp
1.798TrpAla: 1.798 ± 0.386
0.248TrpCys: 0.248 ± 0.133
1.426TrpAsp: 1.426 ± 0.262
1.178TrpGlu: 1.178 ± 0.266
0.93TrpPhe: 0.93 ± 0.24
1.736TrpGly: 1.736 ± 0.312
0.558TrpHis: 0.558 ± 0.191
1.054TrpIle: 1.054 ± 0.213
0.62TrpLys: 0.62 ± 0.243
2.232TrpLeu: 2.232 ± 0.321
0.496TrpMet: 0.496 ± 0.188
0.31TrpAsn: 0.31 ± 0.119
0.744TrpPro: 0.744 ± 0.222
0.868TrpGln: 0.868 ± 0.195
1.178TrpArg: 1.178 ± 0.297
0.93TrpSer: 0.93 ± 0.239
1.736TrpThr: 1.736 ± 0.371
2.17TrpVal: 2.17 ± 0.37
0.62TrpTrp: 0.62 ± 0.217
0.248TrpTyr: 0.248 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.666TyrAla: 2.666 ± 0.493
0.124TyrCys: 0.124 ± 0.083
1.054TyrAsp: 1.054 ± 0.296
2.17TyrGlu: 2.17 ± 0.357
0.496TyrPhe: 0.496 ± 0.149
2.418TyrGly: 2.418 ± 0.463
0.682TyrHis: 0.682 ± 0.206
1.488TyrIle: 1.488 ± 0.34
1.364TyrLys: 1.364 ± 0.287
2.48TyrLeu: 2.48 ± 0.428
0.558TyrMet: 0.558 ± 0.152
1.24TyrAsn: 1.24 ± 0.33
1.426TyrPro: 1.426 ± 0.284
1.054TyrGln: 1.054 ± 0.306
2.48TyrArg: 2.48 ± 0.401
1.55TyrSer: 1.55 ± 0.283
2.046TyrThr: 2.046 ± 0.342
1.488TyrVal: 1.488 ± 0.319
0.31TyrTrp: 0.31 ± 0.132
0.62TyrTyr: 0.62 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (16128 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski