Nocardioides sp. AX2bis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653VG68|A0A653VG68_9ACTN Uncharacterized protein OS=Nocardioides sp. AX2bis OX=2653157 GN=NOCARDAX2BIS_400155 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.43NPEE5 pKa = 3.81RR6 pKa = 11.84RR7 pKa = 11.84GTMKK11 pKa = 9.95TRR13 pKa = 11.84RR14 pKa = 11.84LLATAAVATLALTACSSKK32 pKa = 9.98STDD35 pKa = 3.41SAEE38 pKa = 4.36GGAAAPGVTDD48 pKa = 3.64DD49 pKa = 4.52TISLGVLTDD58 pKa = 3.15QSGPFIQLGTGTVDD72 pKa = 3.78GHH74 pKa = 6.09QIWADD79 pKa = 3.58EE80 pKa = 4.14VNAAGGICGRR90 pKa = 11.84QIEE93 pKa = 4.85LVVRR97 pKa = 11.84DD98 pKa = 4.07HH99 pKa = 7.34GYY101 pKa = 9.67QADD104 pKa = 3.96AGVLAYY110 pKa = 10.29QEE112 pKa = 4.76LLPEE116 pKa = 4.32VLGFMQILGSPVIAALDD133 pKa = 3.38QQLIDD138 pKa = 3.97NEE140 pKa = 4.59TTSVALSWSSFLLKK154 pKa = 10.5NPYY157 pKa = 9.71VVIPGTTYY165 pKa = 10.79DD166 pKa = 3.39VEE168 pKa = 4.55MVNGLSYY175 pKa = 11.11LLEE178 pKa = 4.13QGEE181 pKa = 4.31IAEE184 pKa = 4.49GDD186 pKa = 3.78TVGFVYY192 pKa = 10.92LEE194 pKa = 4.11GEE196 pKa = 4.09YY197 pKa = 11.26GEE199 pKa = 4.95NGLLGAEE206 pKa = 4.45YY207 pKa = 9.71FAEE210 pKa = 4.0QHH212 pKa = 6.97DD213 pKa = 4.12ITLEE217 pKa = 3.98AVKK220 pKa = 10.39VLPTDD225 pKa = 3.22TDD227 pKa = 3.32LRR229 pKa = 11.84NVVTGLRR236 pKa = 11.84GADD239 pKa = 3.3VSAIGLTTTPSQTLSVATVAAQLGLDD265 pKa = 3.68VPLVGNNPTFAPAILTEE282 pKa = 4.23DD283 pKa = 3.14TAAALEE289 pKa = 3.96NYY291 pKa = 9.45YY292 pKa = 10.73LVGSATPYY300 pKa = 10.72SSDD303 pKa = 3.35VPKK306 pKa = 10.56AAEE309 pKa = 3.71VATAYY314 pKa = 10.27EE315 pKa = 4.33EE316 pKa = 4.34LGKK319 pKa = 10.17SEE321 pKa = 4.45PANAGIPYY329 pKa = 9.42GYY331 pKa = 10.41AIGEE335 pKa = 3.72IWGQVLEE342 pKa = 4.65ASCDD346 pKa = 3.53DD347 pKa = 3.75LTRR350 pKa = 11.84SGIQDD355 pKa = 3.71ALASSTDD362 pKa = 3.23VTTDD366 pKa = 3.29EE367 pKa = 5.04LVADD371 pKa = 5.35LDD373 pKa = 4.11LTSPGSPATRR383 pKa = 11.84EE384 pKa = 4.17VYY386 pKa = 10.1IATADD391 pKa = 3.26IDD393 pKa = 4.19AEE395 pKa = 4.57GGLTQQGDD403 pKa = 4.32LFTSPDD409 pKa = 3.49AEE411 pKa = 4.17SYY413 pKa = 9.88VAPEE417 pKa = 4.06EE418 pKa = 4.15QQ419 pKa = 3.43

Molecular weight: 43.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653Q5E6|A0A653Q5E6_9ACTN Dipeptidyl aminopeptidase BI OS=Nocardioides sp. AX2bis OX=2653157 GN=dapb PE=4 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84GLRR6 pKa = 11.84RR7 pKa = 11.84PARR10 pKa = 11.84RR11 pKa = 11.84VGAAVRR17 pKa = 11.84HH18 pKa = 5.92RR19 pKa = 11.84SRR21 pKa = 11.84ALGGPRR27 pKa = 11.84QAAGRR32 pKa = 11.84GRR34 pKa = 11.84QGRR37 pKa = 11.84GRR39 pKa = 11.84GRR41 pKa = 11.84RR42 pKa = 11.84GHH44 pKa = 5.98VLLLRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84QHH53 pKa = 6.79RR54 pKa = 11.84LGLHH58 pKa = 5.62AAGVLGRR65 pKa = 11.84HH66 pKa = 5.4GEE68 pKa = 3.91HH69 pKa = 6.72RR70 pKa = 11.84RR71 pKa = 11.84LAGLRR76 pKa = 11.84RR77 pKa = 11.84GPPGPARR84 pKa = 11.84EE85 pKa = 3.8AHH87 pKa = 5.47RR88 pKa = 11.84RR89 pKa = 11.84GRR91 pKa = 11.84LTRR94 pKa = 11.84TQHH97 pKa = 5.54PRR99 pKa = 11.84RR100 pKa = 11.84PVATSVAAGRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84SRR114 pKa = 11.84TGRR117 pKa = 11.84PGPGAPVHH125 pKa = 6.19

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4362

0

4362

1328517

20

1851

304.6

32.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.489 ± 0.051

0.73 ± 0.011

6.686 ± 0.038

5.594 ± 0.033

2.423 ± 0.026

9.813 ± 0.041

2.252 ± 0.022

2.539 ± 0.029

1.479 ± 0.025

10.353 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.016

1.446 ± 0.018

6.134 ± 0.038

2.663 ± 0.018

8.59 ± 0.065

4.925 ± 0.031

6.097 ± 0.032

9.888 ± 0.044

1.468 ± 0.018

1.747 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski