Thalassotalea sp. ND16A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Thalassotalea; unclassified Thalassotalea

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A099KG70|A0A099KG70_9GAMM HTH lysR-type domain-containing protein OS=Thalassotalea sp. ND16A OX=1535422 GN=ND16A_2254 PE=3 SV=1
MM1 pKa = 7.05KK2 pKa = 10.49QEE4 pKa = 4.01DD5 pKa = 4.58QINKK9 pKa = 8.89DD10 pKa = 2.63TDD12 pKa = 4.21KK13 pKa = 10.81IVKK16 pKa = 5.8PTRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.76FLTRR25 pKa = 11.84TAAGIVVASIPAKK38 pKa = 10.46SVFANNVVNSIIASGHH54 pKa = 5.83ASDD57 pKa = 5.43FAGGDD62 pKa = 3.94PIEE65 pKa = 4.63LLGPCEE71 pKa = 3.99WLEE74 pKa = 3.94QDD76 pKa = 5.13SYY78 pKa = 10.7WADD81 pKa = 3.08ISEE84 pKa = 4.37MPFNSIFGEE93 pKa = 4.69GPGTSLISLLQCFCCDD109 pKa = 3.91DD110 pKa = 4.27PLNFGNDD117 pKa = 3.8DD118 pKa = 5.7DD119 pKa = 6.5DD120 pKa = 6.09DD121 pKa = 5.51HH122 pKa = 9.2DD123 pKa = 4.31NDD125 pKa = 5.05NDD127 pKa = 4.19DD128 pKa = 3.55GHH130 pKa = 8.14DD131 pKa = 4.74NEE133 pKa = 6.5DD134 pKa = 3.86EE135 pKa = 4.43DD136 pKa = 6.26NEE138 pKa = 5.68DD139 pKa = 4.91GDD141 pKa = 6.01DD142 pKa = 5.6DD143 pKa = 6.46GNDD146 pKa = 3.79DD147 pKa = 5.63DD148 pKa = 6.71DD149 pKa = 7.33DD150 pKa = 4.0ITATCADD157 pKa = 3.9DD158 pKa = 4.29NDD160 pKa = 4.31YY161 pKa = 11.51LFAPQMIAMYY171 pKa = 10.48LNAIVHH177 pKa = 6.12YY178 pKa = 7.97NTNGNGPGGLHH189 pKa = 6.16YY190 pKa = 10.19PILQNPEE197 pKa = 4.14DD198 pKa = 4.13LNGYY202 pKa = 7.93IQSLIEE208 pKa = 3.93NRR210 pKa = 11.84FNLAAIIEE218 pKa = 4.38EE219 pKa = 4.35HH220 pKa = 6.69GGNADD225 pKa = 3.39PSSFCNVSGNVFDD238 pKa = 6.71DD239 pKa = 4.52DD240 pKa = 4.71DD241 pKa = 3.8

Molecular weight:
26.32 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A099L9R2|A0A099L9R2_9GAMM Uncharacterized protein OS=Thalassotalea sp. ND16A OX=1535422 GN=ND16A_3934 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.46GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight:
5.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3946

0

3946

1311202

30

6331

332.3

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.622 ± 0.046

1.022 ± 0.017

5.694 ± 0.052

5.791 ± 0.033

4.344 ± 0.029

6.593 ± 0.042

2.191 ± 0.02

6.849 ± 0.034

5.655 ± 0.045

10.099 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.02

5.013 ± 0.032

3.665 ± 0.023

4.785 ± 0.037

3.924 ± 0.03

6.788 ± 0.036

5.49 ± 0.042

6.707 ± 0.035

1.188 ± 0.014

3.155 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski