Amino acid dipepetide frequency for Mycobacterium phage Myrna

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.118AlaAla: 11.118 ± 0.559
0.866AlaCys: 0.866 ± 0.139
6.336AlaAsp: 6.336 ± 0.398
6.612AlaGlu: 6.612 ± 0.404
3.227AlaPhe: 3.227 ± 0.228
7.891AlaGly: 7.891 ± 0.624
1.692AlaHis: 1.692 ± 0.224
3.857AlaIle: 3.857 ± 0.292
4.585AlaLys: 4.585 ± 0.316
7.104AlaLeu: 7.104 ± 0.425
2.44AlaMet: 2.44 ± 0.2
3.758AlaAsn: 3.758 ± 0.299
5.234AlaPro: 5.234 ± 0.473
5.156AlaGln: 5.156 ± 0.322
5.726AlaArg: 5.726 ± 0.381
4.841AlaSer: 4.841 ± 0.276
5.372AlaThr: 5.372 ± 0.356
6.513AlaVal: 6.513 ± 0.383
1.535AlaTrp: 1.535 ± 0.185
2.42AlaTyr: 2.42 ± 0.244
0.0AlaXaa: 0.0 ± 0.0
Cys
0.689CysAla: 0.689 ± 0.135
0.098CysCys: 0.098 ± 0.048
0.669CysAsp: 0.669 ± 0.123
0.492CysGlu: 0.492 ± 0.099
0.256CysPhe: 0.256 ± 0.063
1.004CysGly: 1.004 ± 0.173
0.275CysHis: 0.275 ± 0.072
0.433CysIle: 0.433 ± 0.105
0.413CysLys: 0.413 ± 0.106
0.689CysLeu: 0.689 ± 0.127
0.138CysMet: 0.138 ± 0.056
0.295CysAsn: 0.295 ± 0.089
0.571CysPro: 0.571 ± 0.114
0.256CysGln: 0.256 ± 0.066
0.61CysArg: 0.61 ± 0.132
0.63CysSer: 0.63 ± 0.119
0.472CysThr: 0.472 ± 0.109
0.531CysVal: 0.531 ± 0.121
0.236CysTrp: 0.236 ± 0.072
0.315CysTyr: 0.315 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
5.884AspAla: 5.884 ± 0.347
0.531AspCys: 0.531 ± 0.107
5.313AspAsp: 5.313 ± 0.494
5.175AspGlu: 5.175 ± 0.348
2.44AspPhe: 2.44 ± 0.237
5.352AspGly: 5.352 ± 0.374
1.712AspHis: 1.712 ± 0.177
2.873AspIle: 2.873 ± 0.252
2.479AspLys: 2.479 ± 0.266
4.86AspLeu: 4.86 ± 0.331
1.594AspMet: 1.594 ± 0.194
1.889AspAsn: 1.889 ± 0.217
5.215AspPro: 5.215 ± 0.367
2.971AspGln: 2.971 ± 0.243
4.093AspArg: 4.093 ± 0.305
3.424AspSer: 3.424 ± 0.356
3.64AspThr: 3.64 ± 0.26
4.565AspVal: 4.565 ± 0.27
1.633AspTrp: 1.633 ± 0.224
2.696AspTyr: 2.696 ± 0.267
0.0AspXaa: 0.0 ± 0.0
Glu
6.139GluAla: 6.139 ± 0.455
0.394GluCys: 0.394 ± 0.101
4.447GluAsp: 4.447 ± 0.353
4.585GluGlu: 4.585 ± 0.402
2.46GluPhe: 2.46 ± 0.243
4.447GluGly: 4.447 ± 0.341
1.594GluHis: 1.594 ± 0.196
3.444GluIle: 3.444 ± 0.281
2.952GluLys: 2.952 ± 0.244
4.664GluLeu: 4.664 ± 0.329
1.535GluMet: 1.535 ± 0.202
2.165GluAsn: 2.165 ± 0.185
2.971GluPro: 2.971 ± 0.298
3.326GluGln: 3.326 ± 0.242
4.742GluArg: 4.742 ± 0.329
3.227GluSer: 3.227 ± 0.247
3.444GluThr: 3.444 ± 0.259
4.447GluVal: 4.447 ± 0.337
1.377GluTrp: 1.377 ± 0.178
2.145GluTyr: 2.145 ± 0.217
0.0GluXaa: 0.0 ± 0.0
Phe
2.912PheAla: 2.912 ± 0.257
0.216PheCys: 0.216 ± 0.067
2.558PheAsp: 2.558 ± 0.212
2.027PheGlu: 2.027 ± 0.191
0.885PhePhe: 0.885 ± 0.146
3.03PheGly: 3.03 ± 0.264
0.866PheHis: 0.866 ± 0.146
1.515PheIle: 1.515 ± 0.175
1.633PheLys: 1.633 ± 0.211
2.224PheLeu: 2.224 ± 0.253
1.082PheMet: 1.082 ± 0.144
1.181PheAsn: 1.181 ± 0.156
1.397PhePro: 1.397 ± 0.162
1.338PheGln: 1.338 ± 0.156
1.692PheArg: 1.692 ± 0.19
1.535PheSer: 1.535 ± 0.147
2.086PheThr: 2.086 ± 0.242
1.614PheVal: 1.614 ± 0.19
0.512PheTrp: 0.512 ± 0.114
1.043PheTyr: 1.043 ± 0.14
0.0PheXaa: 0.0 ± 0.0
Gly
6.513GlyAla: 6.513 ± 0.636
0.846GlyCys: 0.846 ± 0.137
5.097GlyAsp: 5.097 ± 0.373
5.116GlyGlu: 5.116 ± 0.418
2.44GlyPhe: 2.44 ± 0.237
9.603GlyGly: 9.603 ± 1.431
1.968GlyHis: 1.968 ± 0.217
3.975GlyIle: 3.975 ± 0.33
3.877GlyLys: 3.877 ± 0.257
5.982GlyLeu: 5.982 ± 0.344
2.145GlyMet: 2.145 ± 0.215
2.893GlyAsn: 2.893 ± 0.289
4.014GlyPro: 4.014 ± 0.416
3.64GlyGln: 3.64 ± 0.276
5.057GlyArg: 5.057 ± 0.39
5.215GlySer: 5.215 ± 0.43
6.277GlyThr: 6.277 ± 0.457
5.588GlyVal: 5.588 ± 0.372
1.732GlyTrp: 1.732 ± 0.188
2.716GlyTyr: 2.716 ± 0.255
0.0GlyXaa: 0.0 ± 0.0
His
2.066HisAla: 2.066 ± 0.203
0.256HisCys: 0.256 ± 0.071
1.22HisAsp: 1.22 ± 0.18
1.476HisGlu: 1.476 ± 0.17
0.787HisPhe: 0.787 ± 0.136
1.712HisGly: 1.712 ± 0.209
0.807HisHis: 0.807 ± 0.158
1.161HisIle: 1.161 ± 0.149
0.728HisLys: 0.728 ± 0.132
1.653HisLeu: 1.653 ± 0.22
0.551HisMet: 0.551 ± 0.109
0.61HisAsn: 0.61 ± 0.115
1.555HisPro: 1.555 ± 0.197
1.043HisGln: 1.043 ± 0.156
1.712HisArg: 1.712 ± 0.183
1.023HisSer: 1.023 ± 0.168
1.004HisThr: 1.004 ± 0.15
1.476HisVal: 1.476 ± 0.183
0.374HisTrp: 0.374 ± 0.107
0.885HisTyr: 0.885 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
4.565IleAla: 4.565 ± 0.314
0.453IleCys: 0.453 ± 0.107
3.601IleAsp: 3.601 ± 0.28
3.345IleGlu: 3.345 ± 0.256
1.122IlePhe: 1.122 ± 0.146
3.601IleGly: 3.601 ± 0.284
0.826IleHis: 0.826 ± 0.121
2.007IleIle: 2.007 ± 0.209
1.81IleLys: 1.81 ± 0.253
2.912IleLeu: 2.912 ± 0.282
0.885IleMet: 0.885 ± 0.124
2.204IleAsn: 2.204 ± 0.207
2.46IlePro: 2.46 ± 0.21
1.81IleGln: 1.81 ± 0.201
3.148IleArg: 3.148 ± 0.271
2.44IleSer: 2.44 ± 0.244
3.266IleThr: 3.266 ± 0.248
3.227IleVal: 3.227 ± 0.25
0.669IleTrp: 0.669 ± 0.159
1.161IleTyr: 1.161 ± 0.143
0.0IleXaa: 0.0 ± 0.0
Lys
4.664LysAla: 4.664 ± 0.372
0.374LysCys: 0.374 ± 0.095
2.696LysAsp: 2.696 ± 0.212
2.401LysGlu: 2.401 ± 0.241
1.24LysPhe: 1.24 ± 0.176
3.444LysGly: 3.444 ± 0.264
0.767LysHis: 0.767 ± 0.13
2.046LysIle: 2.046 ± 0.209
2.44LysLys: 2.44 ± 0.258
3.66LysLeu: 3.66 ± 0.289
0.984LysMet: 0.984 ± 0.148
1.633LysAsn: 1.633 ± 0.187
2.42LysPro: 2.42 ± 0.22
1.515LysGln: 1.515 ± 0.165
3.247LysArg: 3.247 ± 0.317
1.869LysSer: 1.869 ± 0.21
2.637LysThr: 2.637 ± 0.244
3.266LysVal: 3.266 ± 0.279
0.866LysTrp: 0.866 ± 0.147
1.141LysTyr: 1.141 ± 0.165
0.0LysXaa: 0.0 ± 0.0
Leu
7.694LeuAla: 7.694 ± 0.391
0.708LeuCys: 0.708 ± 0.118
5.51LeuAsp: 5.51 ± 0.369
4.172LeuGlu: 4.172 ± 0.34
2.204LeuPhe: 2.204 ± 0.233
5.451LeuGly: 5.451 ± 0.331
1.574LeuHis: 1.574 ± 0.179
2.952LeuIle: 2.952 ± 0.286
2.716LeuLys: 2.716 ± 0.244
4.487LeuLeu: 4.487 ± 0.397
1.987LeuMet: 1.987 ± 0.2
2.322LeuAsn: 2.322 ± 0.219
3.896LeuPro: 3.896 ± 0.26
3.188LeuGln: 3.188 ± 0.305
4.9LeuArg: 4.9 ± 0.328
3.581LeuSer: 3.581 ± 0.324
4.919LeuThr: 4.919 ± 0.399
5.057LeuVal: 5.057 ± 0.347
1.259LeuTrp: 1.259 ± 0.153
2.086LeuTyr: 2.086 ± 0.202
0.0LeuXaa: 0.0 ± 0.0
Met
3.03MetAla: 3.03 ± 0.261
0.118MetCys: 0.118 ± 0.051
1.436MetAsp: 1.436 ± 0.168
1.555MetGlu: 1.555 ± 0.173
0.571MetPhe: 0.571 ± 0.115
2.125MetGly: 2.125 ± 0.213
0.413MetHis: 0.413 ± 0.091
0.925MetIle: 0.925 ± 0.174
0.964MetLys: 0.964 ± 0.144
1.496MetLeu: 1.496 ± 0.157
0.767MetMet: 0.767 ± 0.145
0.964MetAsn: 0.964 ± 0.148
1.555MetPro: 1.555 ± 0.204
0.925MetGln: 0.925 ± 0.147
1.614MetArg: 1.614 ± 0.193
2.145MetSer: 2.145 ± 0.22
2.263MetThr: 2.263 ± 0.221
1.358MetVal: 1.358 ± 0.192
0.453MetTrp: 0.453 ± 0.095
0.512MetTyr: 0.512 ± 0.1
0.0MetXaa: 0.0 ± 0.0
Asn
3.148AsnAla: 3.148 ± 0.283
0.413AsnCys: 0.413 ± 0.108
2.322AsnAsp: 2.322 ± 0.242
2.027AsnGlu: 2.027 ± 0.204
1.023AsnPhe: 1.023 ± 0.136
3.562AsnGly: 3.562 ± 0.307
0.866AsnHis: 0.866 ± 0.113
1.574AsnIle: 1.574 ± 0.179
1.338AsnLys: 1.338 ± 0.192
2.834AsnLeu: 2.834 ± 0.22
0.866AsnMet: 0.866 ± 0.122
1.22AsnAsn: 1.22 ± 0.171
2.893AsnPro: 2.893 ± 0.24
1.358AsnGln: 1.358 ± 0.171
2.42AsnArg: 2.42 ± 0.209
1.496AsnSer: 1.496 ± 0.178
2.007AsnThr: 2.007 ± 0.225
2.578AsnVal: 2.578 ± 0.223
0.866AsnTrp: 0.866 ± 0.15
0.984AsnTyr: 0.984 ± 0.148
0.0AsnXaa: 0.0 ± 0.0
Pro
5.333ProAla: 5.333 ± 0.454
0.512ProCys: 0.512 ± 0.114
4.526ProAsp: 4.526 ± 0.34
4.408ProGlu: 4.408 ± 0.347
1.81ProPhe: 1.81 ± 0.204
5.884ProGly: 5.884 ± 0.464
1.24ProHis: 1.24 ± 0.208
2.066ProIle: 2.066 ± 0.212
2.125ProLys: 2.125 ± 0.297
2.932ProLeu: 2.932 ± 0.238
1.161ProMet: 1.161 ± 0.154
1.909ProAsn: 1.909 ± 0.197
3.463ProPro: 3.463 ± 0.465
2.637ProGln: 2.637 ± 0.243
2.814ProArg: 2.814 ± 0.243
3.05ProSer: 3.05 ± 0.301
3.463ProThr: 3.463 ± 0.275
4.467ProVal: 4.467 ± 0.441
0.846ProTrp: 0.846 ± 0.156
1.653ProTyr: 1.653 ± 0.186
0.0ProXaa: 0.0 ± 0.0
Gln
5.077GlnAla: 5.077 ± 0.362
0.197GlnCys: 0.197 ± 0.06
2.558GlnAsp: 2.558 ± 0.205
2.538GlnGlu: 2.538 ± 0.213
1.515GlnPhe: 1.515 ± 0.189
3.089GlnGly: 3.089 ± 0.336
0.787GlnHis: 0.787 ± 0.141
2.401GlnIle: 2.401 ± 0.202
1.81GlnLys: 1.81 ± 0.169
3.404GlnLeu: 3.404 ± 0.315
1.259GlnMet: 1.259 ± 0.171
1.358GlnAsn: 1.358 ± 0.193
2.283GlnPro: 2.283 ± 0.248
2.145GlnGln: 2.145 ± 0.219
3.326GlnArg: 3.326 ± 0.245
2.381GlnSer: 2.381 ± 0.277
2.283GlnThr: 2.283 ± 0.252
2.952GlnVal: 2.952 ± 0.229
0.728GlnTrp: 0.728 ± 0.123
1.633GlnTyr: 1.633 ± 0.191
0.0GlnXaa: 0.0 ± 0.0
Arg
5.254ArgAla: 5.254 ± 0.369
0.807ArgCys: 0.807 ± 0.145
3.739ArgAsp: 3.739 ± 0.279
4.683ArgGlu: 4.683 ± 0.351
2.106ArgPhe: 2.106 ± 0.243
4.388ArgGly: 4.388 ± 0.365
1.456ArgHis: 1.456 ± 0.218
3.266ArgIle: 3.266 ± 0.278
3.207ArgLys: 3.207 ± 0.271
4.723ArgLeu: 4.723 ± 0.428
1.869ArgMet: 1.869 ± 0.199
2.538ArgAsn: 2.538 ± 0.219
3.109ArgPro: 3.109 ± 0.271
3.05ArgGln: 3.05 ± 0.239
5.49ArgArg: 5.49 ± 0.411
3.188ArgSer: 3.188 ± 0.28
3.955ArgThr: 3.955 ± 0.298
5.018ArgVal: 5.018 ± 0.373
1.299ArgTrp: 1.299 ± 0.154
2.086ArgTyr: 2.086 ± 0.233
0.0ArgXaa: 0.0 ± 0.0
Ser
4.762SerAla: 4.762 ± 0.35
0.374SerCys: 0.374 ± 0.082
3.385SerAsp: 3.385 ± 0.408
2.44SerGlu: 2.44 ± 0.259
1.928SerPhe: 1.928 ± 0.177
5.569SerGly: 5.569 ± 0.324
0.964SerHis: 0.964 ± 0.145
2.932SerIle: 2.932 ± 0.239
1.889SerLys: 1.889 ± 0.216
3.345SerLeu: 3.345 ± 0.261
1.338SerMet: 1.338 ± 0.176
2.046SerAsn: 2.046 ± 0.227
2.971SerPro: 2.971 ± 0.282
2.046SerGln: 2.046 ± 0.208
3.129SerArg: 3.129 ± 0.29
3.404SerSer: 3.404 ± 0.545
3.936SerThr: 3.936 ± 0.273
3.621SerVal: 3.621 ± 0.319
0.984SerTrp: 0.984 ± 0.125
1.968SerTyr: 1.968 ± 0.226
0.0SerXaa: 0.0 ± 0.0
Thr
6.277ThrAla: 6.277 ± 0.437
0.63ThrCys: 0.63 ± 0.117
4.25ThrAsp: 4.25 ± 0.36
3.581ThrGlu: 3.581 ± 0.271
2.027ThrPhe: 2.027 ± 0.223
5.549ThrGly: 5.549 ± 0.384
1.673ThrHis: 1.673 ± 0.17
3.148ThrIle: 3.148 ± 0.273
3.07ThrLys: 3.07 ± 0.246
4.9ThrLeu: 4.9 ± 0.401
1.515ThrMet: 1.515 ± 0.159
2.519ThrAsn: 2.519 ± 0.308
3.896ThrPro: 3.896 ± 0.284
2.538ThrGln: 2.538 ± 0.288
3.601ThrArg: 3.601 ± 0.267
3.05ThrSer: 3.05 ± 0.287
4.349ThrThr: 4.349 ± 0.404
4.467ThrVal: 4.467 ± 0.325
1.102ThrTrp: 1.102 ± 0.168
2.007ThrTyr: 2.007 ± 0.223
0.0ThrXaa: 0.0 ± 0.0
Val
7.241ValAla: 7.241 ± 0.371
0.826ValCys: 0.826 ± 0.148
5.077ValAsp: 5.077 ± 0.283
4.703ValGlu: 4.703 ± 0.299
1.968ValPhe: 1.968 ± 0.179
5.057ValGly: 5.057 ± 0.33
1.436ValHis: 1.436 ± 0.208
3.07ValIle: 3.07 ± 0.259
3.129ValLys: 3.129 ± 0.301
4.919ValLeu: 4.919 ± 0.411
1.751ValMet: 1.751 ± 0.189
2.381ValAsn: 2.381 ± 0.246
4.152ValPro: 4.152 ± 0.378
2.558ValGln: 2.558 ± 0.232
4.191ValArg: 4.191 ± 0.287
3.621ValSer: 3.621 ± 0.279
5.175ValThr: 5.175 ± 0.366
5.451ValVal: 5.451 ± 0.483
1.633ValTrp: 1.633 ± 0.17
2.066ValTyr: 2.066 ± 0.214
0.0ValXaa: 0.0 ± 0.0
Trp
1.594TrpAla: 1.594 ± 0.184
0.216TrpCys: 0.216 ± 0.064
1.358TrpAsp: 1.358 ± 0.168
1.279TrpGlu: 1.279 ± 0.174
0.433TrpPhe: 0.433 ± 0.113
1.24TrpGly: 1.24 ± 0.159
0.394TrpHis: 0.394 ± 0.081
0.689TrpIle: 0.689 ± 0.136
1.063TrpLys: 1.063 ± 0.137
1.476TrpLeu: 1.476 ± 0.178
0.571TrpMet: 0.571 ± 0.092
0.748TrpAsn: 0.748 ± 0.151
0.846TrpPro: 0.846 ± 0.157
0.728TrpGln: 0.728 ± 0.147
1.496TrpArg: 1.496 ± 0.199
1.122TrpSer: 1.122 ± 0.166
1.318TrpThr: 1.318 ± 0.186
1.417TrpVal: 1.417 ± 0.188
0.453TrpTrp: 0.453 ± 0.11
0.689TrpTyr: 0.689 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.322TyrAla: 2.322 ± 0.228
0.236TyrCys: 0.236 ± 0.071
2.243TyrAsp: 2.243 ± 0.253
1.889TyrGlu: 1.889 ± 0.183
0.905TyrPhe: 0.905 ± 0.153
2.696TyrGly: 2.696 ± 0.242
0.787TyrHis: 0.787 ± 0.127
1.181TyrIle: 1.181 ± 0.15
1.102TyrLys: 1.102 ± 0.139
2.401TyrLeu: 2.401 ± 0.236
0.728TyrMet: 0.728 ± 0.151
1.161TyrAsn: 1.161 ± 0.135
1.397TyrPro: 1.397 ± 0.178
1.496TyrGln: 1.496 ± 0.156
2.224TyrArg: 2.224 ± 0.229
1.791TyrSer: 1.791 ± 0.214
2.283TyrThr: 2.283 ± 0.256
2.794TyrVal: 2.794 ± 0.271
0.571TyrTrp: 0.571 ± 0.11
1.023TyrTyr: 1.023 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (50820 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski