Mycobacterium phage Beelzebub

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Marvinvirus; unclassified Marvinvirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FEU8|A0A3G8FEU8_9CAUD Uncharacterized protein OS=Mycobacterium phage Beelzebub OX=2488783 GN=77 PE=4 SV=1
MM1 pKa = 7.56SDD3 pKa = 3.32EE4 pKa = 4.07LRR6 pKa = 11.84AVLTEE11 pKa = 4.1ALAGHH16 pKa = 5.59QPEE19 pKa = 4.6NYY21 pKa = 9.94GFNCSGCNWEE31 pKa = 4.16PANPGVTYY39 pKa = 10.31AAEE42 pKa = 4.25FAAHH46 pKa = 6.25QLDD49 pKa = 3.58VLYY52 pKa = 10.17AVPGVAVIQLPEE64 pKa = 4.88PDD66 pKa = 5.07DD67 pKa = 3.77DD68 pKa = 5.7HH69 pKa = 9.06GDD71 pKa = 4.23GIAFWTAQEE80 pKa = 4.08VGVAGGMVVARR91 pKa = 11.84FDD93 pKa = 3.88PGNEE97 pKa = 3.83AIYY100 pKa = 10.33YY101 pKa = 7.43EE102 pKa = 4.59PSEE105 pKa = 4.06AWEE108 pKa = 4.11TAVALAAAAVVAEE121 pKa = 4.87GEE123 pKa = 4.39DD124 pKa = 3.55KK125 pKa = 11.29

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FHC4|A0A3G8FHC4_9CAUD Helix-turn-helix DNA binding protein OS=Mycobacterium phage Beelzebub OX=2488783 GN=27 PE=4 SV=1
MM1 pKa = 7.44SNRR4 pKa = 11.84RR5 pKa = 11.84TVRR8 pKa = 11.84IRR10 pKa = 11.84IEE12 pKa = 4.03SNKK15 pKa = 10.26AKK17 pKa = 10.11TSFWYY22 pKa = 9.43TLVAGNGNTIMRR34 pKa = 11.84STAKK38 pKa = 9.45YY39 pKa = 4.77TTEE42 pKa = 3.97WNCRR46 pKa = 11.84TAATRR51 pKa = 11.84LCDD54 pKa = 3.71ALRR57 pKa = 11.84FSPLAVEE64 pKa = 4.4VQDD67 pKa = 3.66PKK69 pKa = 10.58TRR71 pKa = 11.84EE72 pKa = 3.43ISTLEE77 pKa = 4.25RR78 pKa = 11.84YY79 pKa = 8.41PSSPLKK85 pKa = 10.56VLQKK89 pKa = 7.78TTQTRR94 pKa = 11.84SS95 pKa = 2.92

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

118

0

118

20738

25

1826

175.7

19.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.77 ± 0.323

1.398 ± 0.194

6.466 ± 0.231

6.77 ± 0.303

3.322 ± 0.161

8.381 ± 0.471

2.194 ± 0.164

4.552 ± 0.154

3.761 ± 0.213

8.193 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.132

3.491 ± 0.182

5.758 ± 0.255

3.699 ± 0.274

6.765 ± 0.383

5.56 ± 0.244

5.56 ± 0.252

6.77 ± 0.237

2.3 ± 0.139

2.657 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski