Microcystis phage vB_MweS-yong1

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LS72|A0A7G8LS72_9VIRU Uncharacterized protein OS=Microcystis phage vB_MweS-yong1 OX=2766836 PE=4 SV=1
MM1 pKa = 7.57IMSKK5 pKa = 10.04WIRR8 pKa = 11.84QPQEE12 pKa = 3.44QEE14 pKa = 3.52GTYY17 pKa = 9.56MFTGHH22 pKa = 6.81FLVTAMVRR30 pKa = 11.84ANLSDD35 pKa = 3.91QEE37 pKa = 4.14IQSIYY42 pKa = 10.96LEE44 pKa = 4.21TQQAVNEE51 pKa = 4.26NNGLDD56 pKa = 3.77YY57 pKa = 11.32LQVFVNRR64 pKa = 11.84EE65 pKa = 3.79TGEE68 pKa = 3.96KK69 pKa = 10.58LFFIASATRR78 pKa = 11.84EE79 pKa = 4.06EE80 pKa = 4.56VEE82 pKa = 4.33SGEE85 pKa = 5.29FDD87 pKa = 3.64PQDD90 pKa = 3.02IDD92 pKa = 3.32YY93 pKa = 9.96HH94 pKa = 7.19NCVLCYY100 pKa = 9.97PEE102 pKa = 4.25EE103 pKa = 4.09RR104 pKa = 4.08

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LS62|A0A7G8LS62_9VIRU Phage/plasmid-like protein OS=Microcystis phage vB_MweS-yong1 OX=2766836 PE=4 SV=1
MM1 pKa = 7.56GLFSDD6 pKa = 4.87IITRR10 pKa = 11.84PNVFLGVQAMSIKK23 pKa = 9.76VGNRR27 pKa = 11.84IRR29 pKa = 11.84LKK31 pKa = 10.02PLQGQHH37 pKa = 5.75VPHH40 pKa = 7.23TYY42 pKa = 10.58YY43 pKa = 10.09IEE45 pKa = 4.41CSRR48 pKa = 11.84KK49 pKa = 9.08EE50 pKa = 3.77RR51 pKa = 11.84GKK53 pKa = 10.97YY54 pKa = 8.67PVGTIFEE61 pKa = 4.83LDD63 pKa = 4.18CILVQPKK70 pKa = 9.64NRR72 pKa = 11.84SAYY75 pKa = 9.8LRR77 pKa = 11.84AIHH80 pKa = 6.27RR81 pKa = 11.84TGLQQSLDD89 pKa = 3.9FFDD92 pKa = 4.71HH93 pKa = 6.5NLRR96 pKa = 11.84LQSFTINQSKK106 pKa = 10.4

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

6024

37

901

261.9

30.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.129 ± 0.589

0.797 ± 0.121

6.109 ± 0.331

7.852 ± 0.421

4.382 ± 0.191

4.399 ± 0.23

1.942 ± 0.27

9.462 ± 0.763

8.831 ± 0.392

8.898 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.409

6.192 ± 0.651

2.855 ± 0.236

4.88 ± 0.358

4.266 ± 0.423

5.943 ± 0.396

5.644 ± 0.321

5.312 ± 0.279

0.664 ± 0.126

4.084 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski