Streptococcus phage Javan558

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BA70|A0A4D6BA70_9CAUD DNA primase/helicase OS=Streptococcus phage Javan558 OX=2548257 GN=Javan558_0036 PE=4 SV=1
MM1 pKa = 8.31DD2 pKa = 6.03DD3 pKa = 3.65PHH5 pKa = 6.88PVPKK9 pKa = 9.65GTLGTVIGVDD19 pKa = 4.24DD20 pKa = 4.44IGSLLVKK27 pKa = 9.7WEE29 pKa = 4.24NGSCLNVLYY38 pKa = 10.78GIDD41 pKa = 3.27IVEE44 pKa = 4.24KK45 pKa = 11.13VMM47 pKa = 4.89

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BBE0|A0A4D6BBE0_9CAUD Endopeptidase OS=Streptococcus phage Javan558 OX=2548257 GN=Javan558_0010 PE=4 SV=1
MM1 pKa = 7.72MYY3 pKa = 10.16TILTCTIMGLWVLIGLYY20 pKa = 9.77FGYY23 pKa = 8.1MTIRR27 pKa = 11.84DD28 pKa = 4.34DD29 pKa = 3.23IRR31 pKa = 11.84NEE33 pKa = 3.63IEE35 pKa = 4.98RR36 pKa = 11.84KK37 pKa = 9.56AKK39 pKa = 9.28QNKK42 pKa = 8.32EE43 pKa = 4.09KK44 pKa = 10.83LSQTPLSRR52 pKa = 11.84KK53 pKa = 9.46NKK55 pKa = 9.68

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

12035

41

1515

279.9

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.813 ± 0.461

0.806 ± 0.152

5.883 ± 0.303

7.179 ± 0.46

3.689 ± 0.221

6.689 ± 0.308

1.886 ± 0.121

6.456 ± 0.315

7.279 ± 0.274

9.007 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.215

4.612 ± 0.198

3.016 ± 0.211

4.678 ± 0.252

4.769 ± 0.269

6.722 ± 0.382

6.747 ± 0.541

6.489 ± 0.236

1.404 ± 0.091

3.606 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski