Rotavirus A human/Vanderbilt/VU08-09-12/2008/G3P[8]

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus G3

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5C726|F5C726_9REOV Non-structural protein 3 OS=Rotavirus A human/Vanderbilt/VU08-09-12/2008/G3P[8] OX=1004874 GN=NSP3 PE=3 SV=1
MM1 pKa = 7.26YY2 pKa = 10.17GIEE5 pKa = 4.26YY6 pKa = 7.25TTVLTFLISVILLNYY21 pKa = 8.65VLKK24 pKa = 10.73SLTRR28 pKa = 11.84IMDD31 pKa = 4.04FIIYY35 pKa = 9.89RR36 pKa = 11.84FLLIIVILSPLLNAQNYY53 pKa = 8.26GINLPITGSMDD64 pKa = 3.38TPYY67 pKa = 10.73TNSTRR72 pKa = 11.84EE73 pKa = 4.09EE74 pKa = 4.15VFLTSTLCLYY84 pKa = 10.75YY85 pKa = 8.86PTEE88 pKa = 4.27AATEE92 pKa = 4.11INDD95 pKa = 3.85NSWKK99 pKa = 9.38DD100 pKa = 3.5TLSQLFLIKK109 pKa = 10.23GWPTGSIYY117 pKa = 10.71FKK119 pKa = 10.4DD120 pKa = 3.75YY121 pKa = 10.94TDD123 pKa = 3.33IASFSVDD130 pKa = 3.05PQLYY134 pKa = 9.83CDD136 pKa = 3.92YY137 pKa = 11.01NLVLMKK143 pKa = 10.45YY144 pKa = 10.21DD145 pKa = 3.39ATLQLDD151 pKa = 3.72MSEE154 pKa = 4.89LADD157 pKa = 3.93LLLNEE162 pKa = 4.55WLCNPMDD169 pKa = 3.29ITLYY173 pKa = 10.67YY174 pKa = 9.25YY175 pKa = 10.55QQTDD179 pKa = 3.57EE180 pKa = 4.17ANKK183 pKa = 8.08WISMGSSCTIKK194 pKa = 10.46VCPLNTQTLGIGCLTTDD211 pKa = 2.98TNTFEE216 pKa = 5.0EE217 pKa = 4.55IATAEE222 pKa = 4.24KK223 pKa = 10.76LVITDD228 pKa = 3.22VVDD231 pKa = 4.33GVNHH235 pKa = 6.78KK236 pKa = 11.16LNVTTNTCTIRR247 pKa = 11.84NCKK250 pKa = 9.83KK251 pKa = 10.18LGPRR255 pKa = 11.84EE256 pKa = 4.01NVAVIQVGGSDD267 pKa = 3.8VLDD270 pKa = 3.44ITADD274 pKa = 3.7PTTMPQTEE282 pKa = 3.54RR283 pKa = 11.84MMRR286 pKa = 11.84VNWKK290 pKa = 9.44KK291 pKa = 7.9WWQVFYY297 pKa = 10.66TIVDD301 pKa = 3.57YY302 pKa = 11.59VNQIVQAMSKK312 pKa = 10.19RR313 pKa = 11.84SRR315 pKa = 11.84SLNSAAFYY323 pKa = 11.0YY324 pKa = 10.27RR325 pKa = 11.84VV326 pKa = 3.19

Molecular weight: 37.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5C724|F5C724_9REOV Non-structural glycoprotein 4 OS=Rotavirus A human/Vanderbilt/VU08-09-12/2008/G3P[8] OX=1004874 GN=NSP4 PE=3 SV=1
MM1 pKa = 7.27NRR3 pKa = 11.84LLQRR7 pKa = 11.84QLFLEE12 pKa = 4.14NLLVGVNSTFHH23 pKa = 6.05QMQKK27 pKa = 10.71HH28 pKa = 6.2SISTCCRR35 pKa = 11.84SLQRR39 pKa = 11.84ILDD42 pKa = 3.95HH43 pKa = 7.28LILLQTIHH51 pKa = 6.63SPAFRR56 pKa = 11.84LDD58 pKa = 3.35RR59 pKa = 11.84MQLRR63 pKa = 11.84QMQTLACLWIHH74 pKa = 6.26RR75 pKa = 11.84HH76 pKa = 4.7NHH78 pKa = 6.09DD79 pKa = 4.37LQATLDD85 pKa = 4.4AINWISPP92 pKa = 3.43

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5892

92

1088

491.0

56.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.82 ± 0.369

1.256 ± 0.383

6.127 ± 0.33

5.244 ± 0.255

4.294 ± 0.306

3.377 ± 0.291

1.612 ± 0.224

8.164 ± 0.298

6.5 ± 0.56

9.318 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.004 ± 0.264

7.451 ± 0.451

3.225 ± 0.228

4.345 ± 0.56

4.667 ± 0.357

7.824 ± 0.795

6.466 ± 0.49

6.195 ± 0.226

1.154 ± 0.138

4.956 ± 0.549
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski