Streptomyces autolyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8Y0G2|A0A1P8Y0G2_9ACTN Prepilin peptidase OS=Streptomyces autolyticus OX=75293 GN=BV401_28195 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84MYY4 pKa = 10.29RR5 pKa = 11.84TMAATATLALALGGAALAAPAAQAAATTSGSLVHH39 pKa = 7.09EE40 pKa = 5.54DD41 pKa = 3.54GEE43 pKa = 4.49LWYY46 pKa = 10.53KK47 pKa = 10.45AAAGQKK53 pKa = 10.0NDD55 pKa = 3.83LTVSEE60 pKa = 4.52QIVNRR65 pKa = 11.84GEE67 pKa = 3.89FEE69 pKa = 3.99SYY71 pKa = 10.72YY72 pKa = 11.25VLTFRR77 pKa = 11.84DD78 pKa = 3.77NGDD81 pKa = 2.93ITIDD85 pKa = 3.49PEE87 pKa = 3.82AANWDD92 pKa = 4.13EE93 pKa = 4.51CVHH96 pKa = 6.44PAAGDD101 pKa = 3.55HH102 pKa = 5.98TVAQCAVEE110 pKa = 4.41IPQNSDD116 pKa = 2.97DD117 pKa = 4.28SDD119 pKa = 4.06NFDD122 pKa = 3.96VDD124 pKa = 5.08LGDD127 pKa = 4.58GNDD130 pKa = 3.65TAKK133 pKa = 10.19IDD135 pKa = 4.28PNGSAYY141 pKa = 10.43AGIHH145 pKa = 6.08GGSGDD150 pKa = 4.63DD151 pKa = 3.7VLQGSAAPTFYY162 pKa = 11.4GEE164 pKa = 4.98DD165 pKa = 3.78GNDD168 pKa = 3.84KK169 pKa = 10.38IDD171 pKa = 3.74GGGGVMGFGAYY182 pKa = 10.05GGDD185 pKa = 3.69GDD187 pKa = 5.4DD188 pKa = 4.88TITNCAQEE196 pKa = 4.37CRR198 pKa = 11.84GGAGNDD204 pKa = 3.8TIVGGSEE211 pKa = 4.08DD212 pKa = 3.67NILRR216 pKa = 11.84GEE218 pKa = 4.2SGDD221 pKa = 4.26DD222 pKa = 3.05ILRR225 pKa = 11.84GGKK228 pKa = 8.19GTDD231 pKa = 3.41AIYY234 pKa = 10.54GGEE237 pKa = 4.91DD238 pKa = 3.41DD239 pKa = 5.41DD240 pKa = 4.4EE241 pKa = 6.37LYY243 pKa = 11.23GEE245 pKa = 4.82EE246 pKa = 5.47GDD248 pKa = 3.76DD249 pKa = 3.47TLYY252 pKa = 11.39GNSGDD257 pKa = 3.79DD258 pKa = 3.93VLWGGQGNDD267 pKa = 3.81TLSGGPGHH275 pKa = 6.62NEE277 pKa = 3.55VHH279 pKa = 6.44QDD281 pKa = 3.01

Molecular weight: 28.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8XYL7|A0A1P8XYL7_9ACTN AraC family transcriptional regulator OS=Streptomyces autolyticus OX=75293 GN=BV401_25590 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7686

0

7686

2766839

29

11273

360.0

38.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.567 ± 0.042

0.782 ± 0.007

5.988 ± 0.022

5.809 ± 0.029

2.699 ± 0.013

9.709 ± 0.028

2.363 ± 0.014

3.287 ± 0.02

2.009 ± 0.024

10.284 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.012

1.706 ± 0.014

6.183 ± 0.031

2.659 ± 0.015

8.226 ± 0.029

5.128 ± 0.024

6.076 ± 0.023

8.266 ± 0.03

1.508 ± 0.012

2.004 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski