Amino acid dipepetide frequency for Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.518AlaAla: 7.518 ± 0.089
0.681AlaCys: 0.681 ± 0.023
4.574AlaAsp: 4.574 ± 0.061
4.824AlaGlu: 4.824 ± 0.068
3.507AlaPhe: 3.507 ± 0.05
6.203AlaGly: 6.203 ± 0.081
1.242AlaHis: 1.242 ± 0.031
5.438AlaIle: 5.438 ± 0.066
4.883AlaLys: 4.883 ± 0.064
7.478AlaLeu: 7.478 ± 0.074
1.792AlaMet: 1.792 ± 0.038
3.91AlaAsn: 3.91 ± 0.059
2.427AlaPro: 2.427 ± 0.039
3.014AlaGln: 3.014 ± 0.045
2.799AlaArg: 2.799 ± 0.046
4.69AlaSer: 4.69 ± 0.085
4.208AlaThr: 4.208 ± 0.081
5.135AlaVal: 5.135 ± 0.068
0.849AlaTrp: 0.849 ± 0.022
3.223AlaTyr: 3.223 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.516CysAla: 0.516 ± 0.024
0.112CysCys: 0.112 ± 0.009
0.367CysAsp: 0.367 ± 0.015
0.354CysGlu: 0.354 ± 0.017
0.436CysPhe: 0.436 ± 0.015
0.569CysGly: 0.569 ± 0.02
0.171CysHis: 0.171 ± 0.013
0.609CysIle: 0.609 ± 0.021
0.538CysLys: 0.538 ± 0.022
0.77CysLeu: 0.77 ± 0.022
0.178CysMet: 0.178 ± 0.009
0.383CysAsn: 0.383 ± 0.016
0.279CysPro: 0.279 ± 0.015
0.213CysGln: 0.213 ± 0.013
0.311CysArg: 0.311 ± 0.014
0.531CysSer: 0.531 ± 0.023
0.432CysThr: 0.432 ± 0.019
0.417CysVal: 0.417 ± 0.017
0.09CysTrp: 0.09 ± 0.008
0.32CysTyr: 0.32 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.938AspAla: 3.938 ± 0.057
0.363AspCys: 0.363 ± 0.015
2.333AspAsp: 2.333 ± 0.05
3.242AspGlu: 3.242 ± 0.051
3.018AspPhe: 3.018 ± 0.046
3.871AspGly: 3.871 ± 0.062
1.009AspHis: 1.009 ± 0.025
3.74AspIle: 3.74 ± 0.046
3.898AspLys: 3.898 ± 0.055
4.998AspLeu: 4.998 ± 0.06
1.165AspMet: 1.165 ± 0.028
2.495AspAsn: 2.495 ± 0.039
2.251AspPro: 2.251 ± 0.042
2.001AspGln: 2.001 ± 0.038
2.116AspArg: 2.116 ± 0.034
2.569AspSer: 2.569 ± 0.047
2.41AspThr: 2.41 ± 0.043
3.425AspVal: 3.425 ± 0.042
0.789AspTrp: 0.789 ± 0.026
2.555AspTyr: 2.555 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
4.239GluAla: 4.239 ± 0.062
0.318GluCys: 0.318 ± 0.015
2.529GluAsp: 2.529 ± 0.045
3.622GluGlu: 3.622 ± 0.064
2.298GluPhe: 2.298 ± 0.039
3.487GluGly: 3.487 ± 0.046
1.045GluHis: 1.045 ± 0.028
4.35GluIle: 4.35 ± 0.067
4.593GluLys: 4.593 ± 0.064
5.802GluLeu: 5.802 ± 0.074
1.476GluMet: 1.476 ± 0.032
3.23GluAsn: 3.23 ± 0.048
1.631GluPro: 1.631 ± 0.033
2.417GluGln: 2.417 ± 0.045
2.491GluArg: 2.491 ± 0.045
2.713GluSer: 2.713 ± 0.046
2.944GluThr: 2.944 ± 0.046
3.761GluVal: 3.761 ± 0.048
0.662GluTrp: 0.662 ± 0.021
1.879GluTyr: 1.879 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.241PheAla: 3.241 ± 0.05
0.465PheCys: 0.465 ± 0.027
2.71PheAsp: 2.71 ± 0.043
2.653PheGlu: 2.653 ± 0.048
2.349PhePhe: 2.349 ± 0.045
3.404PheGly: 3.404 ± 0.051
0.712PheHis: 0.712 ± 0.022
3.496PheIle: 3.496 ± 0.056
3.464PheLys: 3.464 ± 0.056
4.219PheLeu: 4.219 ± 0.058
1.167PheMet: 1.167 ± 0.025
3.109PheAsn: 3.109 ± 0.047
1.764PhePro: 1.764 ± 0.034
1.31PheGln: 1.31 ± 0.029
1.752PheArg: 1.752 ± 0.037
3.743PheSer: 3.743 ± 0.052
3.006PheThr: 3.006 ± 0.052
2.733PheVal: 2.733 ± 0.048
0.595PheTrp: 0.595 ± 0.021
2.078PheTyr: 2.078 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.982GlyAla: 4.982 ± 0.07
0.598GlyCys: 0.598 ± 0.022
3.362GlyAsp: 3.362 ± 0.056
3.232GlyGlu: 3.232 ± 0.046
3.666GlyPhe: 3.666 ± 0.048
5.091GlyGly: 5.091 ± 0.09
1.165GlyHis: 1.165 ± 0.032
5.627GlyIle: 5.627 ± 0.065
5.569GlyLys: 5.569 ± 0.072
6.831GlyLeu: 6.831 ± 0.064
1.745GlyMet: 1.745 ± 0.039
4.113GlyAsn: 4.113 ± 0.079
1.769GlyPro: 1.769 ± 0.037
2.358GlyGln: 2.358 ± 0.039
2.752GlyArg: 2.752 ± 0.042
4.598GlySer: 4.598 ± 0.066
4.774GlyThr: 4.774 ± 0.099
4.371GlyVal: 4.371 ± 0.064
0.944GlyTrp: 0.944 ± 0.028
3.321GlyTyr: 3.321 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.161HisAla: 1.161 ± 0.029
0.196HisCys: 0.196 ± 0.012
0.769HisAsp: 0.769 ± 0.022
0.858HisGlu: 0.858 ± 0.022
1.036HisPhe: 1.036 ± 0.028
1.06HisGly: 1.06 ± 0.029
0.455HisHis: 0.455 ± 0.017
1.328HisIle: 1.328 ± 0.031
1.091HisLys: 1.091 ± 0.023
1.789HisLeu: 1.789 ± 0.036
0.323HisMet: 0.323 ± 0.014
0.895HisAsn: 0.895 ± 0.024
0.922HisPro: 0.922 ± 0.027
0.728HisGln: 0.728 ± 0.022
0.689HisArg: 0.689 ± 0.023
0.952HisSer: 0.952 ± 0.027
1.022HisThr: 1.022 ± 0.024
0.907HisVal: 0.907 ± 0.028
0.237HisTrp: 0.237 ± 0.013
0.808HisTyr: 0.808 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.989IleAla: 5.989 ± 0.063
0.661IleCys: 0.661 ± 0.022
4.095IleAsp: 4.095 ± 0.052
4.084IleGlu: 4.084 ± 0.061
2.986IlePhe: 2.986 ± 0.054
4.981IleGly: 4.981 ± 0.065
1.16IleHis: 1.16 ± 0.027
4.514IleIle: 4.514 ± 0.07
4.968IleLys: 4.968 ± 0.062
6.18IleLeu: 6.18 ± 0.078
1.346IleMet: 1.346 ± 0.03
4.168IleAsn: 4.168 ± 0.068
3.092IlePro: 3.092 ± 0.044
2.129IleGln: 2.129 ± 0.036
3.11IleArg: 3.11 ± 0.043
5.192IleSer: 5.192 ± 0.068
4.373IleThr: 4.373 ± 0.071
4.234IleVal: 4.234 ± 0.06
0.762IleTrp: 0.762 ± 0.023
2.647IleTyr: 2.647 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
5.476LysAla: 5.476 ± 0.059
0.306LysCys: 0.306 ± 0.015
4.309LysAsp: 4.309 ± 0.062
4.581LysGlu: 4.581 ± 0.064
2.58LysPhe: 2.58 ± 0.043
4.838LysGly: 4.838 ± 0.054
1.245LysHis: 1.245 ± 0.031
5.109LysIle: 5.109 ± 0.06
5.581LysLys: 5.581 ± 0.078
6.311LysLeu: 6.311 ± 0.07
1.914LysMet: 1.914 ± 0.037
4.39LysAsn: 4.39 ± 0.05
2.834LysPro: 2.834 ± 0.043
2.705LysGln: 2.705 ± 0.042
2.778LysArg: 2.778 ± 0.046
3.87LysSer: 3.87 ± 0.05
4.386LysThr: 4.386 ± 0.054
4.619LysVal: 4.619 ± 0.061
0.862LysTrp: 0.862 ± 0.023
2.92LysTyr: 2.92 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
7.096LeuAla: 7.096 ± 0.076
0.814LeuCys: 0.814 ± 0.021
4.611LeuAsp: 4.611 ± 0.055
4.798LeuGlu: 4.798 ± 0.069
4.643LeuPhe: 4.643 ± 0.071
6.001LeuGly: 6.001 ± 0.071
1.589LeuHis: 1.589 ± 0.036
6.543LeuIle: 6.543 ± 0.074
7.831LeuLys: 7.831 ± 0.08
9.612LeuLeu: 9.612 ± 0.107
2.272LeuMet: 2.272 ± 0.042
6.098LeuAsn: 6.098 ± 0.066
4.211LeuPro: 4.211 ± 0.059
3.574LeuGln: 3.574 ± 0.045
3.619LeuArg: 3.619 ± 0.055
7.136LeuSer: 7.136 ± 0.075
5.574LeuThr: 5.574 ± 0.068
5.501LeuVal: 5.501 ± 0.072
1.023LeuTrp: 1.023 ± 0.026
3.423LeuTyr: 3.423 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
1.957MetAla: 1.957 ± 0.039
0.137MetCys: 0.137 ± 0.01
1.215MetAsp: 1.215 ± 0.024
1.365MetGlu: 1.365 ± 0.032
0.851MetPhe: 0.851 ± 0.024
1.634MetGly: 1.634 ± 0.036
0.391MetHis: 0.391 ± 0.017
1.476MetIle: 1.476 ± 0.029
2.011MetLys: 2.011 ± 0.038
2.196MetLeu: 2.196 ± 0.037
0.659MetMet: 0.659 ± 0.024
1.311MetAsn: 1.311 ± 0.028
1.091MetPro: 1.091 ± 0.029
0.917MetGln: 0.917 ± 0.023
0.984MetArg: 0.984 ± 0.028
1.326MetSer: 1.326 ± 0.029
1.07MetThr: 1.07 ± 0.024
1.544MetVal: 1.544 ± 0.035
0.176MetTrp: 0.176 ± 0.01
0.725MetTyr: 0.725 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
4.329AsnAla: 4.329 ± 0.055
0.391AsnCys: 0.391 ± 0.019
2.686AsnAsp: 2.686 ± 0.041
2.951AsnGlu: 2.951 ± 0.048
2.689AsnPhe: 2.689 ± 0.042
4.326AsnGly: 4.326 ± 0.061
0.964AsnHis: 0.964 ± 0.026
4.196AsnIle: 4.196 ± 0.054
3.805AsnLys: 3.805 ± 0.054
5.21AsnLeu: 5.21 ± 0.06
1.296AsnMet: 1.296 ± 0.031
3.289AsnAsn: 3.289 ± 0.056
2.796AsnPro: 2.796 ± 0.053
2.007AsnGln: 2.007 ± 0.04
2.343AsnArg: 2.343 ± 0.048
3.323AsnSer: 3.323 ± 0.061
3.469AsnThr: 3.469 ± 0.074
3.328AsnVal: 3.328 ± 0.046
0.818AsnTrp: 0.818 ± 0.026
2.691AsnTyr: 2.691 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
3.616ProAla: 3.616 ± 0.066
0.225ProCys: 0.225 ± 0.012
2.641ProAsp: 2.641 ± 0.042
2.947ProGlu: 2.947 ± 0.048
1.876ProPhe: 1.876 ± 0.037
3.109ProGly: 3.109 ± 0.046
0.614ProHis: 0.614 ± 0.022
2.206ProIle: 2.206 ± 0.041
2.23ProLys: 2.23 ± 0.038
3.545ProLeu: 3.545 ± 0.053
0.753ProMet: 0.753 ± 0.023
1.924ProAsn: 1.924 ± 0.036
0.93ProPro: 0.93 ± 0.027
1.267ProGln: 1.267 ± 0.025
1.183ProArg: 1.183 ± 0.026
2.193ProSer: 2.193 ± 0.04
1.856ProThr: 1.856 ± 0.041
3.41ProVal: 3.41 ± 0.056
0.419ProTrp: 0.419 ± 0.017
1.609ProTyr: 1.609 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.607GlnAla: 2.607 ± 0.046
0.168GlnCys: 0.168 ± 0.011
1.617GlnAsp: 1.617 ± 0.03
2.045GlnGlu: 2.045 ± 0.037
1.562GlnPhe: 1.562 ± 0.028
2.11GlnGly: 2.11 ± 0.036
0.773GlnHis: 0.773 ± 0.023
2.313GlnIle: 2.313 ± 0.039
2.584GlnLys: 2.584 ± 0.045
4.016GlnLeu: 4.016 ± 0.056
0.88GlnMet: 0.88 ± 0.023
1.989GlnAsn: 1.989 ± 0.039
1.418GlnPro: 1.418 ± 0.037
1.972GlnGln: 1.972 ± 0.042
1.492GlnArg: 1.492 ± 0.033
2.078GlnSer: 2.078 ± 0.034
2.086GlnThr: 2.086 ± 0.042
2.385GlnVal: 2.385 ± 0.037
0.435GlnTrp: 0.435 ± 0.025
1.488GlnTyr: 1.488 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
2.632ArgAla: 2.632 ± 0.045
0.226ArgCys: 0.226 ± 0.012
1.899ArgAsp: 1.899 ± 0.035
2.184ArgGlu: 2.184 ± 0.038
2.157ArgPhe: 2.157 ± 0.038
2.349ArgGly: 2.349 ± 0.042
0.663ArgHis: 0.663 ± 0.022
3.154ArgIle: 3.154 ± 0.047
2.896ArgLys: 2.896 ± 0.053
3.91ArgLeu: 3.91 ± 0.049
1.085ArgMet: 1.085 ± 0.025
2.31ArgAsn: 2.31 ± 0.037
1.45ArgPro: 1.45 ± 0.032
1.42ArgGln: 1.42 ± 0.03
1.521ArgArg: 1.521 ± 0.035
2.359ArgSer: 2.359 ± 0.046
2.085ArgThr: 2.085 ± 0.042
2.349ArgVal: 2.349 ± 0.039
0.578ArgTrp: 0.578 ± 0.02
1.932ArgTyr: 1.932 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
4.999SerAla: 4.999 ± 0.068
0.577SerCys: 0.577 ± 0.027
3.023SerAsp: 3.023 ± 0.042
2.729SerGlu: 2.729 ± 0.045
3.612SerPhe: 3.612 ± 0.051
5.276SerGly: 5.276 ± 0.081
0.984SerHis: 0.984 ± 0.027
4.533SerIle: 4.533 ± 0.062
3.922SerLys: 3.922 ± 0.051
6.0SerLeu: 6.0 ± 0.066
1.257SerMet: 1.257 ± 0.029
3.343SerAsn: 3.343 ± 0.055
2.428SerPro: 2.428 ± 0.042
1.83SerGln: 1.83 ± 0.033
2.565SerArg: 2.565 ± 0.037
4.225SerSer: 4.225 ± 0.071
3.557SerThr: 3.557 ± 0.057
4.122SerVal: 4.122 ± 0.054
0.895SerTrp: 0.895 ± 0.024
2.866SerTyr: 2.866 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
5.317ThrAla: 5.317 ± 0.105
0.352ThrCys: 0.352 ± 0.015
3.461ThrAsp: 3.461 ± 0.05
3.142ThrGlu: 3.142 ± 0.043
2.676ThrPhe: 2.676 ± 0.044
5.084ThrGly: 5.084 ± 0.089
0.918ThrHis: 0.918 ± 0.024
4.119ThrIle: 4.119 ± 0.064
3.14ThrLys: 3.14 ± 0.047
5.616ThrLeu: 5.616 ± 0.069
1.018ThrMet: 1.018 ± 0.023
2.785ThrAsn: 2.785 ± 0.055
2.475ThrPro: 2.475 ± 0.052
1.774ThrGln: 1.774 ± 0.032
2.03ThrArg: 2.03 ± 0.035
3.478ThrSer: 3.478 ± 0.067
3.501ThrThr: 3.501 ± 0.077
4.0ThrVal: 4.0 ± 0.096
0.682ThrTrp: 0.682 ± 0.02
2.449ThrTyr: 2.449 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
4.791ValAla: 4.791 ± 0.068
0.568ValCys: 0.568 ± 0.018
3.137ValAsp: 3.137 ± 0.045
3.197ValGlu: 3.197 ± 0.048
3.215ValPhe: 3.215 ± 0.048
3.622ValGly: 3.622 ± 0.053
1.036ValHis: 1.036 ± 0.028
4.646ValIle: 4.646 ± 0.062
4.887ValLys: 4.887 ± 0.057
6.281ValLeu: 6.281 ± 0.069
1.543ValMet: 1.543 ± 0.033
3.827ValAsn: 3.827 ± 0.06
2.614ValPro: 2.614 ± 0.047
2.107ValGln: 2.107 ± 0.039
2.24ValArg: 2.24 ± 0.041
4.395ValSer: 4.395 ± 0.062
3.764ValThr: 3.764 ± 0.095
4.216ValVal: 4.216 ± 0.067
0.698ValTrp: 0.698 ± 0.025
2.622ValTyr: 2.622 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.838TrpAla: 0.838 ± 0.026
0.116TrpCys: 0.116 ± 0.008
0.659TrpAsp: 0.659 ± 0.024
0.722TrpGlu: 0.722 ± 0.019
0.563TrpPhe: 0.563 ± 0.021
0.864TrpGly: 0.864 ± 0.025
0.232TrpHis: 0.232 ± 0.012
0.752TrpIle: 0.752 ± 0.024
0.902TrpLys: 0.902 ± 0.025
1.215TrpLeu: 1.215 ± 0.031
0.375TrpMet: 0.375 ± 0.016
0.758TrpAsn: 0.758 ± 0.024
0.39TrpPro: 0.39 ± 0.017
0.533TrpGln: 0.533 ± 0.018
0.487TrpArg: 0.487 ± 0.019
0.698TrpSer: 0.698 ± 0.024
0.684TrpThr: 0.684 ± 0.027
0.73TrpVal: 0.73 ± 0.021
0.205TrpTrp: 0.205 ± 0.013
0.526TrpTyr: 0.526 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.152TyrAla: 3.152 ± 0.049
0.322TyrCys: 0.322 ± 0.017
2.189TyrAsp: 2.189 ± 0.041
1.957TyrGlu: 1.957 ± 0.035
2.253TyrPhe: 2.253 ± 0.043
2.988TyrGly: 2.988 ± 0.049
0.856TyrHis: 0.856 ± 0.023
2.523TyrIle: 2.523 ± 0.041
2.824TyrLys: 2.824 ± 0.047
3.989TyrLeu: 3.989 ± 0.057
0.792TyrMet: 0.792 ± 0.022
2.58TyrAsn: 2.58 ± 0.045
1.779TyrPro: 1.779 ± 0.032
1.742TyrGln: 1.742 ± 0.034
1.898TyrArg: 1.898 ± 0.044
2.675TyrSer: 2.675 ± 0.043
2.815TyrThr: 2.815 ± 0.06
2.217TyrVal: 2.217 ± 0.036
0.53TyrTrp: 0.53 ± 0.017
1.86TyrTyr: 1.86 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4249 proteins (1593950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski