Synechococcus phage S-RIM2 R1_1999

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Nerrivikvirus; Synechococcus virus SRIM2

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4PMB4|M4PMB4_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIM2 R1_1999 OX=869662 GN=SWTG_00081 PE=4 SV=1
MM1 pKa = 7.43PQFTLICTDD10 pKa = 3.25EE11 pKa = 5.05DD12 pKa = 4.05STVTTKK18 pKa = 10.68EE19 pKa = 3.89FEE21 pKa = 4.19ATVLDD26 pKa = 4.1DD27 pKa = 5.55VVDD30 pKa = 3.75KK31 pKa = 10.24TQDD34 pKa = 3.19FLKK37 pKa = 10.98GVGYY41 pKa = 10.38CFEE44 pKa = 4.24EE45 pKa = 4.61LHH47 pKa = 5.63TQVYY51 pKa = 8.4PLPEE55 pKa = 5.11SNDD58 pKa = 3.51DD59 pKa = 4.36DD60 pKa = 4.47YY61 pKa = 12.22SSIYY65 pKa = 10.19RR66 pKa = 11.84DD67 pKa = 2.86VDD69 pKa = 3.2

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4PMD6|M4PMD6_9CAUD Uncharacterized protein OS=Synechococcus phage S-RIM2 R1_1999 OX=869662 GN=SWTG_00111 PE=4 SV=1
MM1 pKa = 7.33FKK3 pKa = 10.77ALTKK7 pKa = 10.38SRR9 pKa = 11.84PAKK12 pKa = 10.16FYY14 pKa = 10.07SNQVKK19 pKa = 10.2VLLLIIIGAMLWSNNDD35 pKa = 2.9ARR37 pKa = 11.84HH38 pKa = 4.91FTADD42 pKa = 3.67RR43 pKa = 11.84LQDD46 pKa = 3.21ASNLVRR52 pKa = 11.84PNNTQFTISFF62 pKa = 3.98

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

210

0

210

56089

45

6378

267.1

29.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.228 ± 0.186

0.916 ± 0.101

6.67 ± 0.154

6.163 ± 0.295

4.265 ± 0.117

7.93 ± 0.348

1.471 ± 0.115

6.035 ± 0.167

5.73 ± 0.412

7.171 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.12 ± 0.216

5.841 ± 0.212

3.798 ± 0.137

3.755 ± 0.111

4.161 ± 0.174

6.882 ± 0.224

7.661 ± 0.461

6.85 ± 0.207

1.182 ± 0.088

4.172 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski