Vibrio phage vB_pir03

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8AMR8|A0A7G8AMR8_9CAUD Uncharacterized protein OS=Vibrio phage vB_pir03 OX=2762667 PE=4 SV=1
MM1 pKa = 7.46LTTIIYY7 pKa = 9.6TNIFQDD13 pKa = 3.21TDD15 pKa = 3.07KK16 pKa = 10.83GLEE19 pKa = 3.86VAAFYY24 pKa = 10.61AQEE27 pKa = 4.32GNQEE31 pKa = 4.77IGLEE35 pKa = 3.85WLGNINYY42 pKa = 8.43TLGCVMAQLRR52 pKa = 11.84VRR54 pKa = 11.84SNPTKK59 pKa = 10.62AVTVLIDD66 pKa = 5.55LIQQYY71 pKa = 10.35IDD73 pKa = 3.33SDD75 pKa = 4.31FNFADD80 pKa = 3.3VDD82 pKa = 3.96TSFVGQDD89 pKa = 3.42YY90 pKa = 9.68MSVEE94 pKa = 4.57VIHH97 pKa = 6.79ALQAVLEE104 pKa = 4.26LDD106 pKa = 4.49PYY108 pKa = 11.29AEE110 pKa = 4.03DD111 pKa = 2.91WFYY114 pKa = 11.77QHH116 pKa = 7.0LFDD119 pKa = 5.66AAAEE123 pKa = 4.21SEE125 pKa = 4.14EE126 pKa = 4.78GEE128 pKa = 4.41DD129 pKa = 4.52IEE131 pKa = 4.54YY132 pKa = 10.78LKK134 pKa = 10.89PLVV137 pKa = 3.66

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8AMM9|A0A7G8AMM9_9CAUD Uncharacterized protein OS=Vibrio phage vB_pir03 OX=2762667 PE=4 SV=1
MM1 pKa = 6.95GQALRR6 pKa = 11.84LIRR9 pKa = 11.84NLLPSGRR16 pKa = 11.84RR17 pKa = 11.84SLFLPFQKK25 pKa = 10.48KK26 pKa = 9.85HH27 pKa = 6.12ISILLL32 pKa = 3.8

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

334

0

334

89868

29

6417

269.1

30.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.586 ± 0.229

1.055 ± 0.055

6.362 ± 0.134

6.757 ± 0.209

4.603 ± 0.22

6.294 ± 0.167

2.18 ± 0.11

6.121 ± 0.188

5.853 ± 0.252

8.339 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.038 ± 0.086

5.036 ± 0.23

3.865 ± 0.094

3.294 ± 0.093

4.981 ± 0.177

6.299 ± 0.129

6.535 ± 0.207

6.891 ± 0.103

1.348 ± 0.107

3.565 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski