Podiceps cristatus (Great crested grebe)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7899 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094LC80|A0A094LC80_PODCR Calcium-binding mitochondrial carrier protein SCaMC-2 (Fragment) OS=Podiceps cristatus OX=345573 GN=N338_02319 PE=3 SV=1
QQ1 pKa = 7.33GSYY4 pKa = 10.62HH5 pKa = 7.07CGQCKK10 pKa = 9.99AGYY13 pKa = 8.56TGDD16 pKa = 3.59QVRR19 pKa = 11.84GCQAEE24 pKa = 4.04RR25 pKa = 11.84SCRR28 pKa = 11.84NRR30 pKa = 11.84ALNPCSVHH38 pKa = 5.15AHH40 pKa = 6.53CIEE43 pKa = 3.83EE44 pKa = 4.03RR45 pKa = 11.84RR46 pKa = 11.84GEE48 pKa = 4.25VTCICGIGWAGDD60 pKa = 3.82GYY62 pKa = 10.67ICGKK66 pKa = 10.36DD67 pKa = 3.5VDD69 pKa = 3.63IDD71 pKa = 4.16GYY73 pKa = 10.1PNEE76 pKa = 4.55EE77 pKa = 4.13LSCSAEE83 pKa = 3.8NCRR86 pKa = 11.84KK87 pKa = 10.08DD88 pKa = 3.11NCRR91 pKa = 11.84FVPNSGQEE99 pKa = 4.02DD100 pKa = 4.12ADD102 pKa = 3.79GDD104 pKa = 4.61GIGDD108 pKa = 4.09ACDD111 pKa = 4.46DD112 pKa = 4.56DD113 pKa = 6.96ADD115 pKa = 4.36GDD117 pKa = 4.52GIPNEE122 pKa = 4.01QDD124 pKa = 3.11NCVLAPNVNQRR135 pKa = 11.84NSDD138 pKa = 3.11QDD140 pKa = 3.43IFGDD144 pKa = 3.72ACDD147 pKa = 3.38NCRR150 pKa = 11.84NVLNNDD156 pKa = 3.07QRR158 pKa = 11.84DD159 pKa = 3.45TDD161 pKa = 3.87GDD163 pKa = 4.16GKK165 pKa = 11.42GDD167 pKa = 3.74ACDD170 pKa = 5.17DD171 pKa = 4.26DD172 pKa = 4.15MDD174 pKa = 6.26GDD176 pKa = 4.5GIKK179 pKa = 10.77NLLDD183 pKa = 3.4NCQRR187 pKa = 11.84FPNQDD192 pKa = 3.3QEE194 pKa = 5.61DD195 pKa = 3.97KK196 pKa = 11.46DD197 pKa = 3.9NDD199 pKa = 4.31GVGDD203 pKa = 4.14ACDD206 pKa = 3.44SCPTVSNPNQSDD218 pKa = 3.32VDD220 pKa = 3.63NDD222 pKa = 4.07LVGDD226 pKa = 4.11SCDD229 pKa = 3.63TNQDD233 pKa = 3.04SDD235 pKa = 4.07GDD237 pKa = 4.03GHH239 pKa = 7.17QDD241 pKa = 3.22STDD244 pKa = 3.33NCPTIINSSQLDD256 pKa = 3.76TDD258 pKa = 4.01KK259 pKa = 11.41DD260 pKa = 4.0GPDD263 pKa = 3.37NCRR266 pKa = 11.84LVPNPGQEE274 pKa = 4.18DD275 pKa = 4.25DD276 pKa = 4.42NGDD279 pKa = 3.69GVGDD283 pKa = 3.48ICEE286 pKa = 4.29SDD288 pKa = 3.45FDD290 pKa = 3.86QDD292 pKa = 3.88TVIDD296 pKa = 4.99RR297 pKa = 11.84IDD299 pKa = 3.38VCPEE303 pKa = 3.33NAEE306 pKa = 3.78ITLTDD311 pKa = 3.83FRR313 pKa = 11.84AYY315 pKa = 8.19QTVVLDD321 pKa = 4.22PEE323 pKa = 5.32GDD325 pKa = 3.65AQIDD329 pKa = 4.13PNWVVLNQGMEE340 pKa = 4.11IVQTMNSDD348 pKa = 3.19PGLAVGYY355 pKa = 7.31TAFNGVDD362 pKa = 3.99FEE364 pKa = 4.97GTFHH368 pKa = 6.81VNTVTDD374 pKa = 3.57DD375 pKa = 4.08DD376 pKa = 4.35YY377 pKa = 12.0AGFIFGYY384 pKa = 9.58QDD386 pKa = 2.83SSSFYY391 pKa = 10.47VVMWKK396 pKa = 7.78QTEE399 pKa = 3.86QTYY402 pKa = 8.13WQATPFRR409 pKa = 11.84AVAEE413 pKa = 4.19PGIQLKK419 pKa = 10.18VLL421 pKa = 3.67

Molecular weight: 45.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094NFK6|A0A094NFK6_PODCR LYR motif-containing protein 9 (Fragment) OS=Podiceps cristatus OX=345573 GN=N338_06851 PE=4 SV=1
KKK2 pKa = 10.12RR3 pKa = 11.84QGKKK7 pKa = 8.85RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.73RR48 pKa = 11.84QGKKK52 pKa = 8.73RR53 pKa = 11.84QGKKK57 pKa = 9.07RR58 pKa = 11.84

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7899

0

7899

3087637

31

5432

390.9

43.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.36 ± 0.023

2.242 ± 0.023

5.031 ± 0.019

7.17 ± 0.04

3.984 ± 0.02

5.803 ± 0.032

2.54 ± 0.013

5.024 ± 0.022

6.505 ± 0.035

9.705 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.014

4.187 ± 0.021

5.232 ± 0.031

4.637 ± 0.025

5.179 ± 0.024

8.232 ± 0.044

5.401 ± 0.024

6.297 ± 0.022

1.191 ± 0.011

3.005 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski