Hubei sobemo-like virus 28

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEJ3|A0A1L3KEJ3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 28 OX=1923214 PE=4 SV=1
MM1 pKa = 7.58GEE3 pKa = 4.35AIIDD7 pKa = 3.59RR8 pKa = 11.84AVQQHH13 pKa = 6.25RR14 pKa = 11.84SARR17 pKa = 11.84WVIPEE22 pKa = 4.11DD23 pKa = 4.39FISRR27 pKa = 11.84AHH29 pKa = 7.11FDD31 pKa = 3.29RR32 pKa = 11.84VLQKK36 pKa = 10.99LDD38 pKa = 3.5MQSSPGYY45 pKa = 8.33PYY47 pKa = 10.58CVRR50 pKa = 11.84IPVNAHH56 pKa = 6.14LFGVNADD63 pKa = 3.95GEE65 pKa = 4.35KK66 pKa = 11.05DD67 pKa = 3.18PDD69 pKa = 3.41AVEE72 pKa = 4.4RR73 pKa = 11.84VWEE76 pKa = 4.21AVQHH80 pKa = 6.04RR81 pKa = 11.84LGKK84 pKa = 10.55LLDD87 pKa = 4.04GEE89 pKa = 4.55EE90 pKa = 4.37DD91 pKa = 3.75CDD93 pKa = 5.25PIRR96 pKa = 11.84LFIKK100 pKa = 10.27PEE102 pKa = 3.73PLKK105 pKa = 10.56EE106 pKa = 4.15KK107 pKa = 10.61KK108 pKa = 9.26LTEE111 pKa = 3.48HH112 pKa = 7.77RR113 pKa = 11.84YY114 pKa = 10.01RR115 pKa = 11.84IISSVSIVDD124 pKa = 3.61QIIDD128 pKa = 3.66HH129 pKa = 7.01LLFDD133 pKa = 5.29DD134 pKa = 4.59MNEE137 pKa = 3.65TMYY140 pKa = 11.44ANWHH144 pKa = 5.8NVPSMVGWSPFVGGWKK160 pKa = 10.17VMPKK164 pKa = 10.32LKK166 pKa = 9.92WLAIDD171 pKa = 3.63KK172 pKa = 10.74SSWDD176 pKa = 3.36WTVHH180 pKa = 5.71KK181 pKa = 10.13WILDD185 pKa = 3.32QCLEE189 pKa = 4.07LRR191 pKa = 11.84TRR193 pKa = 11.84LCDD196 pKa = 3.55NMNAAWLKK204 pKa = 9.66LARR207 pKa = 11.84ARR209 pKa = 11.84YY210 pKa = 8.46HH211 pKa = 6.82LLYY214 pKa = 10.63DD215 pKa = 3.58NPVLVTSGGVLLRR228 pKa = 11.84QKK230 pKa = 9.53TPGVMKK236 pKa = 10.13SGCVNTIADD245 pKa = 3.82NSIAQYY251 pKa = 11.38LLDD254 pKa = 4.13LRR256 pKa = 11.84VCFEE260 pKa = 4.14IGLTPGYY267 pKa = 9.86LMSMGDD273 pKa = 3.76DD274 pKa = 3.56TLQQKK279 pKa = 9.57PRR281 pKa = 11.84DD282 pKa = 3.66LQKK285 pKa = 11.29YY286 pKa = 9.68LDD288 pKa = 4.48ALSSFCIVKK297 pKa = 10.09QATEE301 pKa = 3.97ANEE304 pKa = 3.82FAGMLFNGRR313 pKa = 11.84NVEE316 pKa = 3.89PLYY319 pKa = 10.58HH320 pKa = 6.71GKK322 pKa = 8.97HH323 pKa = 5.84ACTILHH329 pKa = 7.07AKK331 pKa = 10.15PEE333 pKa = 4.17ILEE336 pKa = 4.2EE337 pKa = 4.02LADD340 pKa = 4.62AYY342 pKa = 9.88TLLYY346 pKa = 10.22HH347 pKa = 7.14RR348 pKa = 11.84SKK350 pKa = 10.48FRR352 pKa = 11.84NWFEE356 pKa = 4.12NLFTAMGIDD365 pKa = 3.79FHH367 pKa = 6.69PRR369 pKa = 11.84WKK371 pKa = 10.23RR372 pKa = 11.84DD373 pKa = 3.53AIYY376 pKa = 10.74DD377 pKa = 3.93GEE379 pKa = 4.33DD380 pKa = 2.86

Molecular weight: 43.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEJ3|A0A1L3KEJ3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 28 OX=1923214 PE=4 SV=1
MM1 pKa = 6.58TTNSEE6 pKa = 4.1QIYY9 pKa = 10.16NPLSEE14 pKa = 4.53EE15 pKa = 4.06VEE17 pKa = 4.04AQPRR21 pKa = 11.84CGSWLSRR28 pKa = 11.84ALKK31 pKa = 9.24WVLAVLVAAVTVPLIWSAFCGLMIGLSGWVLGWYY65 pKa = 7.71RR66 pKa = 11.84TQHH69 pKa = 5.5LALYY73 pKa = 8.82EE74 pKa = 3.97LAKK77 pKa = 10.73NAFGPPPSPPEE88 pKa = 3.93PDD90 pKa = 3.4VVEE93 pKa = 4.85QYY95 pKa = 10.66AQKK98 pKa = 11.03LLDD101 pKa = 3.52FAEE104 pKa = 4.72RR105 pKa = 11.84VDD107 pKa = 3.87STLLATLLVAIIMVISIWITFGLLMRR133 pKa = 11.84SLRR136 pKa = 11.84RR137 pKa = 11.84ISYY140 pKa = 9.35KK141 pKa = 10.42LRR143 pKa = 11.84GIHH146 pKa = 6.51LEE148 pKa = 3.94SMQPGSDD155 pKa = 3.5FTASDD160 pKa = 3.39IPSYY164 pKa = 8.08QVKK167 pKa = 10.08VLSPGLLGDD176 pKa = 3.56SHH178 pKa = 7.76QGYY181 pKa = 9.68GIRR184 pKa = 11.84VGSWLVVPLHH194 pKa = 5.82VLNSVSEE201 pKa = 4.23LMLQGPTGQKK211 pKa = 9.03MVMRR215 pKa = 11.84KK216 pKa = 8.3QYY218 pKa = 10.21EE219 pKa = 4.23VSRR222 pKa = 11.84LHH224 pKa = 7.25ADD226 pKa = 3.13VAYY229 pKa = 10.55LYY231 pKa = 10.72LEE233 pKa = 4.29KK234 pKa = 10.68EE235 pKa = 4.13QWSKK239 pKa = 11.56LGAKK243 pKa = 8.55NAKK246 pKa = 6.94TASMCRR252 pKa = 11.84GFARR256 pKa = 11.84CYY258 pKa = 10.19GPRR261 pKa = 11.84GYY263 pKa = 11.65SMGSMDD269 pKa = 4.12RR270 pKa = 11.84SGLFGMLVYY279 pKa = 10.57SGSTIPGMSGAAYY292 pKa = 9.6EE293 pKa = 4.34LMGKK297 pKa = 9.41VVGLHH302 pKa = 5.78TGCTNTTNMGVSAALFVEE320 pKa = 4.36EE321 pKa = 5.76LMTKK325 pKa = 9.43TPLKK329 pKa = 10.27EE330 pKa = 3.99EE331 pKa = 4.17SASALGISSGVGEE344 pKa = 4.23QSAAVYY350 pKa = 10.8SLGSKK355 pKa = 9.58IGKK358 pKa = 9.37QIEE361 pKa = 4.25AWDD364 pKa = 3.5KK365 pKa = 10.36AQIKK369 pKa = 9.61EE370 pKa = 4.12RR371 pKa = 11.84LANPDD376 pKa = 3.08SRR378 pKa = 11.84PWHH381 pKa = 6.65EE382 pKa = 5.42DD383 pKa = 3.11DD384 pKa = 5.97EE385 pKa = 5.67IDD387 pKa = 3.88YY388 pKa = 11.0DD389 pKa = 3.7QDD391 pKa = 3.9LGLEE395 pKa = 4.37SASKK399 pKa = 10.07PKK401 pKa = 10.52KK402 pKa = 10.02LVPLGIVQQPLLLQSDD418 pKa = 4.24SPKK421 pKa = 11.11EE422 pKa = 4.0EE423 pKa = 4.12VLTLADD429 pKa = 3.7VTGLAARR436 pKa = 11.84VTALEE441 pKa = 3.95EE442 pKa = 4.38CVRR445 pKa = 11.84EE446 pKa = 4.03LQQLAIKK453 pKa = 10.18KK454 pKa = 9.55KK455 pKa = 10.36AEE457 pKa = 3.94KK458 pKa = 10.5QEE460 pKa = 4.59FKK462 pKa = 11.29CPDD465 pKa = 3.51CDD467 pKa = 3.35IACRR471 pKa = 11.84TEE473 pKa = 4.35LRR475 pKa = 11.84LSNHH479 pKa = 6.66LKK481 pKa = 8.28FTCAKK486 pKa = 9.54RR487 pKa = 11.84VSFDD491 pKa = 4.43CEE493 pKa = 3.93LCEE496 pKa = 4.46TKK498 pKa = 10.59CRR500 pKa = 11.84TAEE503 pKa = 3.81TLKK506 pKa = 10.06RR507 pKa = 11.84HH508 pKa = 5.4MVNSHH513 pKa = 5.17TKK515 pKa = 9.54VKK517 pKa = 10.5IEE519 pKa = 4.35GEE521 pKa = 4.0SAYY524 pKa = 10.01PGDD527 pKa = 3.9SKK529 pKa = 11.6VVVQTGPFLAKK540 pKa = 10.04RR541 pKa = 11.84RR542 pKa = 11.84ASQKK546 pKa = 9.73GRR548 pKa = 11.84KK549 pKa = 7.32KK550 pKa = 8.65TSSRR554 pKa = 11.84SSSSSGDD561 pKa = 3.22RR562 pKa = 11.84SRR564 pKa = 11.84SPSLEE569 pKa = 3.87EE570 pKa = 3.87TLSLMTSSLLRR581 pKa = 11.84IEE583 pKa = 4.69KK584 pKa = 10.34SLNKK588 pKa = 10.13NQGDD592 pKa = 3.8TAGQSLATMLNN603 pKa = 3.57

Molecular weight: 66.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

983

380

603

491.5

55.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.325 ± 0.279

2.136 ± 0.018

5.392 ± 1.45

6.205 ± 0.393

2.645 ± 0.602

6.714 ± 0.688

2.442 ± 0.72

4.578 ± 0.702

6.307 ± 0.3

11.495 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.154 ± 0.002

2.848 ± 0.637

4.578 ± 0.092

3.967 ± 0.317

5.493 ± 0.324

8.24 ± 2.029

4.578 ± 0.67

6.612 ± 0.172

2.238 ± 0.533

3.052 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski