Streptococcus phage Javan263

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AA73|A0A4D6AA73_9CAUD Cys_rich_CPCC domain-containing protein OS=Streptococcus phage Javan263 OX=2548080 GN=Javan263_0031 PE=4 SV=1
MM1 pKa = 6.75QHH3 pKa = 6.51GLFGDD8 pKa = 4.1FDD10 pKa = 4.73YY11 pKa = 11.58DD12 pKa = 3.43NWLSTYY18 pKa = 9.72EE19 pKa = 3.77DD20 pKa = 3.72HH21 pKa = 7.85EE22 pKa = 4.83EE23 pKa = 4.21VFHH26 pKa = 7.75CDD28 pKa = 2.6EE29 pKa = 5.27DD30 pKa = 3.7EE31 pKa = 6.18AYY33 pKa = 10.47DD34 pKa = 3.79RR35 pKa = 11.84WKK37 pKa = 10.85DD38 pKa = 3.68DD39 pKa = 4.57QLEE42 pKa = 4.15DD43 pKa = 3.19WW44 pKa = 4.52

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A6M4|A0A4D6A6M4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan263 OX=2548080 GN=Javan263_0038 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 8.63YY4 pKa = 8.24TRR6 pKa = 11.84PDD8 pKa = 2.48RR9 pKa = 11.84HH10 pKa = 5.97GPHH13 pKa = 6.21RR14 pKa = 11.84VAFEE18 pKa = 4.11KK19 pKa = 10.65NKK21 pKa = 10.5KK22 pKa = 10.13VILKK26 pKa = 8.01TRR28 pKa = 11.84NVCGICGKK36 pKa = 9.69PVDD39 pKa = 4.28KK40 pKa = 10.64SLKK43 pKa = 8.99YY44 pKa = 8.55PHH46 pKa = 7.28PLSPVIDD53 pKa = 4.59HH54 pKa = 7.44IIPVAKK60 pKa = 10.01GGHH63 pKa = 6.66PSDD66 pKa = 4.31IDD68 pKa = 3.76NLQLAHH74 pKa = 5.98WQCNRR79 pKa = 11.84QKK81 pKa = 10.99SDD83 pKa = 2.92KK84 pKa = 10.43LYY86 pKa = 10.84NDD88 pKa = 4.12EE89 pKa = 4.14PSKK92 pKa = 11.45GKK94 pKa = 8.32GTNVIGNRR102 pKa = 11.84NLPQTINWLNYY113 pKa = 8.8VSKK116 pKa = 11.14

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10304

39

1565

206.1

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.046 ± 0.507

0.66 ± 0.111

6.396 ± 0.292

7.288 ± 0.611

3.746 ± 0.218

6.192 ± 0.492

1.475 ± 0.151

6.716 ± 0.351

8.084 ± 0.563

8.142 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.219

6.405 ± 0.254

2.824 ± 0.222

4.047 ± 0.256

3.61 ± 0.337

6.891 ± 0.489

6.619 ± 0.673

6.357 ± 0.197

1.339 ± 0.136

3.892 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski