Plasmodium berghei (strain Anka)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Vinckeia); Plasmodium berghei

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509AEL7|A0A509AEL7_PLABA Cleavage and polyadenylation specificity factor subunit 5 OS=Plasmodium berghei (strain Anka) OX=5823 GN=PBANKA_0204400 PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.54NNIMKK7 pKa = 10.15ISFALLFLSSYY18 pKa = 10.39FSEE21 pKa = 4.22INGNVTNKK29 pKa = 9.93NKK31 pKa = 9.88YY32 pKa = 8.92RR33 pKa = 11.84LRR35 pKa = 11.84SYY37 pKa = 8.98LTKK40 pKa = 10.85NEE42 pKa = 4.16DD43 pKa = 4.5LNNTSYY49 pKa = 11.27NGNIHH54 pKa = 6.63GEE56 pKa = 4.21SEE58 pKa = 4.2DD59 pKa = 4.2AISVGDD65 pKa = 3.62TTQTLKK71 pKa = 11.03NGEE74 pKa = 4.51SGTQGSSNLPQGKK87 pKa = 8.98SLQSTDD93 pKa = 3.59TNHH96 pKa = 6.11SQDD99 pKa = 2.57GHH101 pKa = 6.33TEE103 pKa = 4.01DD104 pKa = 4.56SCAEE108 pKa = 4.14CGRR111 pKa = 11.84GCTEE115 pKa = 3.92GCEE118 pKa = 4.48GCHH121 pKa = 6.66DD122 pKa = 4.57ASCLEE127 pKa = 4.1CHH129 pKa = 6.64EE130 pKa = 4.78GCEE133 pKa = 4.53DD134 pKa = 5.23CPDD137 pKa = 3.84CHH139 pKa = 7.71DD140 pKa = 4.79EE141 pKa = 4.81SCLDD145 pKa = 3.87CQTEE149 pKa = 4.26CTDD152 pKa = 3.24ACRR155 pKa = 11.84EE156 pKa = 3.85EE157 pKa = 4.29CAKK160 pKa = 10.55IKK162 pKa = 10.06TDD164 pKa = 3.25TVHH167 pKa = 7.51RR168 pKa = 11.84DD169 pKa = 3.28GEE171 pKa = 4.44NVQACTQCTTNDD183 pKa = 4.0PNCEE187 pKa = 3.97EE188 pKa = 4.25CKK190 pKa = 10.68KK191 pKa = 10.6IITTRR196 pKa = 11.84QPEE199 pKa = 4.43TPNSGIEE206 pKa = 3.98AEE208 pKa = 4.27PASVVQNAIEE218 pKa = 5.86DD219 pKa = 3.73ITNDD223 pKa = 3.58TKK225 pKa = 11.12TMPDD229 pKa = 3.39EE230 pKa = 4.44NEE232 pKa = 4.14AVCDD236 pKa = 3.78EE237 pKa = 4.13QLDD240 pKa = 3.81EE241 pKa = 5.56GGDD244 pKa = 3.67NEE246 pKa = 4.25EE247 pKa = 4.88EE248 pKa = 3.9EE249 pKa = 5.58DD250 pKa = 3.92IVDD253 pKa = 4.16SEE255 pKa = 4.68DD256 pKa = 3.48IEE258 pKa = 5.03IIEE261 pKa = 4.22HH262 pKa = 6.07TGDD265 pKa = 4.38IEE267 pKa = 5.59DD268 pKa = 4.41SDD270 pKa = 4.43DD271 pKa = 4.14SEE273 pKa = 4.34EE274 pKa = 5.09LEE276 pKa = 5.12LEE278 pKa = 4.31LEE280 pKa = 4.49LEE282 pKa = 4.2LEE284 pKa = 4.38EE285 pKa = 6.48AIDD288 pKa = 3.77MSRR291 pKa = 11.84DD292 pKa = 3.47GQEE295 pKa = 3.95EE296 pKa = 4.49NEE298 pKa = 4.59GEE300 pKa = 4.37TGTTTTNEE308 pKa = 3.48QRR310 pKa = 11.84NNHH313 pKa = 5.05NTNTNEE319 pKa = 3.94VTDD322 pKa = 3.97NNQTNNSNNTNNNNNGNHH340 pKa = 6.97FIIKK344 pKa = 9.34EE345 pKa = 3.96YY346 pKa = 10.57KK347 pKa = 9.8GNHH350 pKa = 5.6DD351 pKa = 4.05AKK353 pKa = 10.39TEE355 pKa = 3.97NEE357 pKa = 4.26NILNNALSDD366 pKa = 3.39IDD368 pKa = 5.29DD369 pKa = 4.02NQFSSDD375 pKa = 3.4IQDD378 pKa = 3.8FAQDD382 pKa = 3.0INEE385 pKa = 4.11YY386 pKa = 11.12VLMEE390 pKa = 4.23EE391 pKa = 5.59DD392 pKa = 3.1II393 pKa = 4.75

Molecular weight: 43.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509AIL1|A0A509AIL1_PLABA AP2 domain transcription factor putative OS=Plasmodium berghei (strain Anka) OX=5823 GN=PBANKA_0932300 PE=4 SV=1
MM1 pKa = 7.28AHH3 pKa = 6.31GASRR7 pKa = 11.84YY8 pKa = 8.75KK9 pKa = 10.48KK10 pKa = 10.3SRR12 pKa = 11.84AKK14 pKa = 9.88MRR16 pKa = 11.84WKK18 pKa = 9.16WKK20 pKa = 9.29KK21 pKa = 9.72KK22 pKa = 6.67RR23 pKa = 11.84TRR25 pKa = 11.84RR26 pKa = 11.84LQKK29 pKa = 9.69KK30 pKa = 7.67RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 8.24MRR35 pKa = 11.84QRR37 pKa = 11.84SRR39 pKa = 3.22

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4927

1

4928

3405970

29

9556

691.1

81.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.251 ± 0.018

1.702 ± 0.012

5.616 ± 0.023

7.093 ± 0.04

4.897 ± 0.03

3.007 ± 0.025

1.936 ± 0.011

10.203 ± 0.038

11.926 ± 0.039

7.976 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.884 ± 0.01

13.28 ± 0.07

2.086 ± 0.021

2.729 ± 0.017

2.557 ± 0.017

7.038 ± 0.03

4.203 ± 0.021

3.539 ± 0.018

0.497 ± 0.008

5.58 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski