Solemya velum gill symbiont

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2657 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B0H5F3|A0A0B0H5F3_SOVGS 50S ribosomal protein L15 OS=Solemya velum gill symbiont OX=2340 GN=rplO PE=3 SV=1
MM1 pKa = 6.88NHH3 pKa = 6.88KK4 pKa = 10.7ASLNQNKK11 pKa = 9.62SVEE14 pKa = 4.26PNSLQGMRR22 pKa = 11.84RR23 pKa = 11.84TATAIAVASVLSVATLPQATAGFSYY48 pKa = 11.02GSGDD52 pKa = 3.69TIAITGPVTAHH63 pKa = 6.29NNDD66 pKa = 2.9GSDD69 pKa = 3.46LVNVNAYY76 pKa = 9.68FADD79 pKa = 5.4DD80 pKa = 3.21ISGSYY85 pKa = 10.48GVLLRR90 pKa = 11.84GDD92 pKa = 4.08SGASSNTITNNGYY105 pKa = 9.93DD106 pKa = 3.72ADD108 pKa = 4.16VSGSAAAIEE117 pKa = 4.41VGYY120 pKa = 10.46IPGPDD125 pKa = 3.48DD126 pKa = 4.1VRR128 pKa = 11.84AHH130 pKa = 6.54NNTITNSGRR139 pKa = 11.84MEE141 pKa = 4.4GSGGRR146 pKa = 11.84AVPTGRR152 pKa = 11.84TGVAPRR158 pKa = 11.84IVITPPGTYY167 pKa = 9.45LQYY170 pKa = 10.85EE171 pKa = 4.63GTVHH175 pKa = 5.58ITAFAAEE182 pKa = 4.41NNTDD186 pKa = 3.39ADD188 pKa = 3.97AEE190 pKa = 4.22VDD192 pKa = 3.54NNLIEE197 pKa = 4.22NSGTISGDD205 pKa = 2.8GTLAVHH211 pKa = 6.82LGSFAYY217 pKa = 10.59VSGSSAHH224 pKa = 6.86KK225 pKa = 9.48GTAEE229 pKa = 3.84VGNNSVNNSGGIVSDD244 pKa = 3.54STGVQFLSIAGGFSGTHH261 pKa = 6.41AEE263 pKa = 4.03ATVNDD268 pKa = 3.85NVVSNAYY275 pKa = 10.38DD276 pKa = 3.5GDD278 pKa = 4.1INGGMLSGIDD288 pKa = 3.5MTAIAATSSSCCGPQGNADD307 pKa = 3.44ASVEE311 pKa = 4.38GNSNTNDD318 pKa = 3.3GYY320 pKa = 11.53AGDD323 pKa = 4.48SLLSGASMNAIAASGYY339 pKa = 9.48MPFARR344 pKa = 11.84ADD346 pKa = 3.49AEE348 pKa = 4.57VNSNEE353 pKa = 3.65ITNAEE358 pKa = 3.98NANIEE363 pKa = 4.15GKK365 pKa = 10.87YY366 pKa = 10.64NGIALRR372 pKa = 11.84AAGEE376 pKa = 4.29DD377 pKa = 2.8IKK379 pKa = 11.43YY380 pKa = 10.78AGDD383 pKa = 3.46VAVNSNMISNDD394 pKa = 2.81GTVFASEE401 pKa = 4.36DD402 pKa = 3.46QFADD406 pKa = 3.64SAVKK410 pKa = 9.98LASYY414 pKa = 11.08GNLANMFSKK423 pKa = 10.77DD424 pKa = 4.13DD425 pKa = 3.6GHH427 pKa = 6.74GLPFANDD434 pKa = 3.05TDD436 pKa = 4.35VNSNTITNSSTGDD449 pKa = 3.52MVNSAWMVRR458 pKa = 11.84DD459 pKa = 3.39SAVSLRR465 pKa = 11.84ADD467 pKa = 3.07SSTGDD472 pKa = 3.24RR473 pKa = 11.84YY474 pKa = 9.82DD475 pKa = 3.54TPFSGSSSAEE485 pKa = 3.88VNSNSIEE492 pKa = 3.77NHH494 pKa = 4.91GFIGSFAQYY503 pKa = 10.77NFGGGIEE510 pKa = 4.11LTADD514 pKa = 3.38AGGDD518 pKa = 3.36AYY520 pKa = 10.12EE521 pKa = 4.12YY522 pKa = 10.67DD523 pKa = 4.41YY524 pKa = 11.21ISSGGFIDD532 pKa = 3.4HH533 pKa = 6.75EE534 pKa = 4.25EE535 pKa = 4.41RR536 pKa = 11.84IGVSAGTADD545 pKa = 3.6VNKK548 pKa = 8.84NTVSNTSSDD557 pKa = 3.75TIAVGGYY564 pKa = 8.99VLFDD568 pKa = 3.82ADD570 pKa = 5.01GIEE573 pKa = 4.33LNADD577 pKa = 3.29ASASAEE583 pKa = 4.19TYY585 pKa = 10.33IDD587 pKa = 3.2ISGGFSDD594 pKa = 5.07ASGSADD600 pKa = 3.08SLAFAGDD607 pKa = 3.31ADD609 pKa = 4.05VNNNQVEE616 pKa = 4.27NSGSIEE622 pKa = 3.76VTGYY626 pKa = 9.55MGEE629 pKa = 4.15DD630 pKa = 3.14SQGIVLAADD639 pKa = 3.44AWAYY643 pKa = 10.87ANSDD647 pKa = 3.12VTAQSSGSSSADD659 pKa = 3.23SFGMASGGTTFVNNNDD675 pKa = 3.92VINSGDD681 pKa = 3.41ILTSGEE687 pKa = 4.56DD688 pKa = 3.66FPSQYY693 pKa = 9.34MQGILLTSSASAFAYY708 pKa = 10.53SEE710 pKa = 4.04ATAVSGGGNGDD721 pKa = 3.43ASGGATAMAGTAEE734 pKa = 4.03VHH736 pKa = 6.16NNNVVNSGTISTDD749 pKa = 3.33GEE751 pKa = 4.19DD752 pKa = 3.23SSGIEE757 pKa = 3.68LSAFAWADD765 pKa = 3.04ADD767 pKa = 3.72VDD769 pKa = 4.3LSGSSTTDD777 pKa = 2.58SDD779 pKa = 3.61GTTASGGFASVANNTITNSGDD800 pKa = 2.55ITTAGGRR807 pKa = 11.84GASSTGILLYY817 pKa = 10.85ADD819 pKa = 3.66VSAWAEE825 pKa = 4.03RR826 pKa = 11.84FSDD829 pKa = 3.84DD830 pKa = 3.95TDD832 pKa = 4.16GSDD835 pKa = 3.19VARR838 pKa = 11.84AGIAEE843 pKa = 4.06ITGNSVDD850 pKa = 3.44NSGVIDD856 pKa = 3.44AWEE859 pKa = 4.97GIVLEE864 pKa = 4.47ARR866 pKa = 11.84ASAFADD872 pKa = 3.22NYY874 pKa = 10.31ARR876 pKa = 11.84SGEE879 pKa = 4.23AYY881 pKa = 9.24LTDD884 pKa = 3.46NTITNSGTIIADD896 pKa = 3.4NVGIAIRR903 pKa = 11.84GEE905 pKa = 3.92ASGSVINTIFTHH917 pKa = 6.34GNSVDD922 pKa = 3.41NSGSMEE928 pKa = 4.14VYY930 pKa = 9.6NGSGIIVGEE939 pKa = 4.11EE940 pKa = 4.03APSGSGSYY948 pKa = 8.53TWDD951 pKa = 3.08NTINNTGTILAGEE964 pKa = 4.21DD965 pKa = 3.53TAIGIYY971 pKa = 10.53GDD973 pKa = 3.45AEE975 pKa = 4.66YY976 pKa = 10.72YY977 pKa = 10.89GSGGSGSSGSNTLILGAPGFIAGDD1001 pKa = 3.92IILDD1005 pKa = 3.53GDD1007 pKa = 4.2ANVDD1011 pKa = 3.47VTLTSGAGNSVAWTFDD1027 pKa = 3.5DD1028 pKa = 4.23SGSNGPSLPVMTDD1041 pKa = 3.25GPLPWFTDD1049 pKa = 3.76SNSGGSTWATIDD1061 pKa = 3.28PTAFAAAHH1069 pKa = 5.54NQVADD1074 pKa = 4.04LAGMASGMQFGSMGQDD1090 pKa = 3.28DD1091 pKa = 4.58SGMASGDD1098 pKa = 3.63PMTSMDD1104 pKa = 6.52DD1105 pKa = 3.34GGLWLAIEE1113 pKa = 4.87AGEE1116 pKa = 4.54SDD1118 pKa = 3.69YY1119 pKa = 11.8DD1120 pKa = 4.01GDD1122 pKa = 4.88GVTTLDD1128 pKa = 4.54QEE1130 pKa = 4.57TTLTGIAAGYY1140 pKa = 8.01SAKK1143 pKa = 10.47LNPEE1147 pKa = 3.94TRR1149 pKa = 11.84VGAMLGYY1156 pKa = 9.93GKK1158 pKa = 10.17SEE1160 pKa = 4.62LEE1162 pKa = 3.78VDD1164 pKa = 4.03SNIGIATVKK1173 pKa = 10.33SYY1175 pKa = 10.96EE1176 pKa = 4.14SEE1178 pKa = 3.7QDD1180 pKa = 3.7GIFGGIYY1187 pKa = 10.23GRR1189 pKa = 11.84AKK1191 pKa = 10.43LGSIYY1196 pKa = 10.73LDD1198 pKa = 3.46LALNAGWQSHH1208 pKa = 4.87DD1209 pKa = 3.52QARR1212 pKa = 11.84LVNDD1216 pKa = 3.31NLQNDD1221 pKa = 4.18GTDD1224 pKa = 3.8FANASYY1230 pKa = 11.27DD1231 pKa = 3.9SFWFSPEE1238 pKa = 3.58IGVMLPIMVSDD1249 pKa = 4.23TLTVTPNLRR1258 pKa = 11.84ARR1260 pKa = 11.84YY1261 pKa = 8.93VSQSIDD1267 pKa = 3.05GYY1269 pKa = 7.85TEE1271 pKa = 3.99KK1272 pKa = 10.89DD1273 pKa = 3.59SNSNATVDD1281 pKa = 4.84DD1282 pKa = 3.57IDD1284 pKa = 4.42IGVTEE1289 pKa = 4.69LRR1291 pKa = 11.84GGLDD1295 pKa = 3.17VTHH1298 pKa = 6.55RR1299 pKa = 11.84TSVMALTGTIGYY1311 pKa = 7.4MSRR1314 pKa = 11.84SGSGDD1319 pKa = 3.25DD1320 pKa = 3.75SVNITMILDD1329 pKa = 3.83NNDD1332 pKa = 2.68VGFNYY1337 pKa = 10.38SDD1339 pKa = 2.91VDD1341 pKa = 3.37AAYY1344 pKa = 10.41GGISFAYY1351 pKa = 9.29KK1352 pKa = 9.8MNEE1355 pKa = 3.48ATTLKK1360 pKa = 10.5LNASVTVGDD1369 pKa = 5.45DD1370 pKa = 2.76IDD1372 pKa = 4.02AASASASVNMKK1383 pKa = 9.87FF1384 pKa = 4.46

Molecular weight: 140.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B0HBU6|A0A0B0HBU6_SOVGS EAL/GGDEF domain-containing protein OS=Solemya velum gill symbiont OX=2340 GN=JV46_11790 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.5RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.95GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42GGRR28 pKa = 11.84KK29 pKa = 9.17VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84ARR41 pKa = 11.84LAGG44 pKa = 3.63

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2657

0

2657

796070

19

2861

299.6

33.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.036 ± 0.052

1.018 ± 0.02

5.898 ± 0.042

6.902 ± 0.054

3.767 ± 0.034

7.485 ± 0.038

2.377 ± 0.026

6.168 ± 0.034

4.515 ± 0.045

10.287 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.023

3.496 ± 0.031

4.328 ± 0.029

3.82 ± 0.031

5.614 ± 0.036

6.293 ± 0.043

5.144 ± 0.026

6.986 ± 0.043

1.276 ± 0.024

2.765 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski